##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290291_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82754272
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08111515983126	37.0	37.0	37.0	37.0	37.0
2	36.1453962280038	37.0	37.0	37.0	37.0	37.0
3	36.243856595101214	37.0	37.0	37.0	37.0	37.0
4	36.22109053173714	37.0	37.0	37.0	37.0	37.0
5	36.184051779224156	37.0	37.0	37.0	37.0	37.0
6	36.107998170777215	37.0	37.0	37.0	37.0	37.0
7	36.068742034248096	37.0	37.0	37.0	37.0	37.0
8	36.136807825461865	37.0	37.0	37.0	37.0	37.0
9	36.08797733124883	37.0	37.0	37.0	37.0	37.0
10-11	36.113772356066406	37.0	37.0	37.0	37.0	37.0
12-13	36.09512325236817	37.0	37.0	37.0	37.0	37.0
14-15	36.08694845385142	37.0	37.0	37.0	37.0	37.0
16-17	36.071630193302894	37.0	37.0	37.0	37.0	37.0
18-19	36.07882764046308	37.0	37.0	37.0	37.0	37.0
20-21	36.068741798610716	37.0	37.0	37.0	37.0	37.0
22-23	36.04115151904182	37.0	37.0	37.0	37.0	37.0
24-25	36.02876604968502	37.0	37.0	37.0	37.0	37.0
26-27	35.99888276462634	37.0	37.0	37.0	37.0	37.0
28-29	35.9825568642547	37.0	37.0	37.0	37.0	37.0
30-31	35.96339555739189	37.0	37.0	37.0	37.0	37.0
32-33	35.94328108523509	37.0	37.0	37.0	37.0	37.0
34-35	35.92610163376218	37.0	37.0	37.0	37.0	37.0
36-37	35.920150220447425	37.0	37.0	37.0	37.0	37.0
38-39	35.90844809644936	37.0	37.0	37.0	37.0	37.0
40-41	35.912695508275476	37.0	37.0	37.0	37.0	37.0
42-43	35.87439356167886	37.0	37.0	37.0	37.0	37.0
44-45	35.862311513430484	37.0	37.0	37.0	37.0	37.0
46-47	35.86608345038749	37.0	37.0	37.0	37.0	37.0
48-49	35.84640443722503	37.0	37.0	37.0	37.0	37.0
50-51	35.828803099483	37.0	37.0	37.0	37.0	37.0
52-53	35.81591474487921	37.0	37.0	37.0	37.0	37.0
54-55	35.81150242646564	37.0	37.0	37.0	37.0	37.0
56-57	35.780280891156366	37.0	37.0	37.0	37.0	37.0
58-59	35.80091286302964	37.0	37.0	37.0	37.0	37.0
60-61	35.76865602206733	37.0	37.0	37.0	37.0	37.0
62-63	35.75646925941559	37.0	37.0	37.0	37.0	37.0
64-65	35.74240934179806	37.0	37.0	37.0	37.0	37.0
66-67	35.72618502633845	37.0	37.0	37.0	37.0	37.0
68-69	35.690699126061205	37.0	37.0	37.0	37.0	37.0
70-71	35.69940224565393	37.0	37.0	37.0	37.0	37.0
72-73	35.678235347493086	37.0	37.0	37.0	37.0	37.0
74-75	35.69091733578467	37.0	37.0	37.0	37.0	37.0
76-77	35.66686137073826	37.0	37.0	37.0	37.0	37.0
78-79	35.620585684558975	37.0	37.0	37.0	37.0	37.0
80-81	35.617069053946054	37.0	37.0	37.0	37.0	37.0
82-83	35.58531698980741	37.0	37.0	37.0	37.0	37.0
84-85	35.54479251164001	37.0	37.0	37.0	37.0	37.0
86-87	35.5089047066618	37.0	37.0	37.0	37.0	37.0
88-89	35.53768662620644	37.0	37.0	37.0	37.0	37.0
90-91	35.47962024193078	37.0	37.0	37.0	37.0	37.0
92-93	35.52496423146893	37.0	37.0	37.0	37.0	37.0
94-95	35.47903738384531	37.0	37.0	37.0	37.0	37.0
96-97	35.45716555563659	37.0	37.0	37.0	37.0	37.0
98-99	35.452159283081826	37.0	37.0	37.0	37.0	37.0
100-101	35.43285320198803	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25117.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	34.0
12	1162.0
13	10464.0
14	23751.0
15	27821.0
16	28164.0
17	31756.0
18	32534.0
19	31529.0
20	36054.0
21	51297.0
22	74646.0
23	105277.0
24	135497.0
25	173027.0
26	214607.0
27	285109.0
28	372896.0
29	507593.0
30	685382.0
31	990214.0
32	1453874.0
33	2435151.0
34	4543732.0
35	1.1874636E7
36	4.8912854E7
37	9690094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.230150270621017	16.238334761066042	11.021018992400437	46.510495975912505
2	22.47717344208967	19.35688749900397	32.388128924110376	25.77781013479598
3	23.649712131467822	19.97299530030517	23.35076217871692	33.026530389510086
4	25.090970281087333	25.236362447812226	20.78972480953579	28.882942461564653
5	26.259110547818732	29.07073829532212	23.004246035689405	21.665905121169743
6	21.752398016225808	33.60203207708581	23.155413325535246	21.490156581153137
7	19.163346383832533	22.067345865417902	37.82041412804607	20.948893622703498
8	19.957638368412116	24.062110730315922	29.595976899703754	26.384274001568215
9	20.948825368140476	22.141027817110277	31.73128423385062	25.178862580898624
10-11	22.898128419272375	30.529231042039513	24.084781742392863	22.487858796295246
12-13	22.292694973488693	25.8411387905363	27.494310846715752	24.37185538925925
14-15	22.320553491080737	26.452270661879734	26.888662251019706	24.33851359601982
16-17	23.147557197816706	26.415939888081237	26.335028821481327	24.101474092620734
18-19	22.936951902313833	26.775778542915024	25.844877498598013	24.44239205617313
20-21	22.96682719882337	26.602163418512554	26.165821270021915	24.265188112642157
22-23	22.821446926463793	26.696393578054924	26.347166465300486	24.134993030180798
24-25	22.81274502139197	26.483579757078097	26.436318667299403	24.267356554230535
26-27	22.8698396816771	26.759454070392913	26.274559570739207	24.09614667719078
28-29	22.74073502761979	26.562597221138418	26.256796601909087	24.439871149332696
30-31	22.789917859209766	26.301792185116007	26.422617687871963	24.485672267802265
32-33	23.003383718414558	26.505407785620072	26.281663963963165	24.20954453200221
34-35	22.97893121463563	26.60331233373574	26.073466434790415	24.344290016838215
36-37	22.717023432010762	26.541686238357393	26.29054253669713	24.450747792934713
38-39	23.028369381819136	26.643369760456224	26.35408884624596	23.97417201147867
40-41	23.21575713363931	26.520860011121368	25.953890215141094	24.309492640098227
42-43	22.938375955410823	26.572631595670927	26.19219353768981	24.296798911228436
44-45	23.057384382081395	26.505849970077872	26.361793664565887	24.07497198327485
46-47	23.026728437878464	26.257647493229403	26.3698435350653	24.345780533826833
48-49	22.838599918433676	26.125263717093166	26.471140376980735	24.564995987492424
50-51	23.083164970531577	26.113773999596784	26.361736272915227	24.441324756956416
52-53	23.34834999421816	26.404557519113176	26.045688034653168	24.2014044520155
54-55	22.874251685994235	26.357082276189743	26.350523989399267	24.41814204841676
56-57	23.014522799135108	26.50745551688708	26.234482608838583	24.243539075139232
58-59	23.10154950354236	26.394760672667378	26.163664064264857	24.340025759525403
60-61	23.132156222163623	26.255368160536165	26.142725644383034	24.46974997291718
62-63	23.224900086531193	26.160648489760263	26.232592439667357	24.381858984041184
64-65	23.264416629515765	26.345883007078097	25.98186068357966	24.407839679826477
66-67	23.316317865952907	26.231496628621652	26.070316082729732	24.381869422695708
68-69	23.264197595249296	26.385619482335965	26.23488625061504	24.115296671799697
70-71	23.5570769177663	26.323643058270175	26.044866618688328	24.0744134052752
72-73	23.33560446108023	26.229688794497687	26.05455567722357	24.380151067198515
74-75	23.366660113172482	26.399453444874823	26.051482619801998	24.1824038221507
76-77	23.384289827605684	26.36040756461965	26.01482288378373	24.240479723990944
78-79	23.20692657374504	26.371223446201437	25.904713522749734	24.51713645730379
80-81	23.409499392129156	26.364122582008005	25.976474119755867	24.24990390610697
82-83	23.4620634103531	26.394253493988053	25.746768293253147	24.396914802405696
84-85	23.365401725632342	26.424323758700137	25.87166828275614	24.338606232911385
86-87	23.349141557511818	26.4245448854934	25.88523303896934	24.34108051802544
88-89	23.546177883560603	26.174878404268643	25.807272174632917	24.47167153753784
90-91	23.430575827593138	26.174752149723364	26.055716108358812	24.33895591432469
92-93	23.71105378202512	26.133381672400617	25.97619841515359	24.17936613042067
94-95	23.544202231716344	26.206349412404183	26.008579083182465	24.240869272697008
96-97	23.551711310556136	25.992237822386965	26.045337133336144	24.410713733720755
98-99	23.70613318562092	25.21685907819538	26.520996282456306	24.556011453727393
100-101	24.69244402381749	11.725428220159438	32.47060420381172	31.111523552211352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25312.0
1	12750.0
2	304.5
3	615.0
4	1027.5
5	1400.0
6	1747.5
7	2085.0
8	2403.5
9	2670.5
10	3019.5
11	3419.5
12	3699.5
13	4049.0
14	4388.5
15	4754.0
16	5302.0
17	5961.5
18	6779.0
19	7891.0
20	9245.5
21	11295.0
22	14716.0
23	19551.5
24	25503.5
25	33589.0
26	43650.5
27	59951.0
28	82338.5
29	108578.5
30	148360.5
31	201509.5
32	285934.0
33	398229.5
34	528347.5
35	694905.0
36	934301.5
37	1246761.5
38	1628844.5
39	2142601.0
40	2641300.5
41	3084942.0
42	3589193.5
43	4096636.5
44	4493885.0
45	4776080.5
46	4920821.5
47	4941238.0
48	4821712.0
49	4601021.0
50	4311057.5
51	3992227.5
52	3570195.0
53	3007487.5
54	2537645.5
55	2207445.5
56	1937174.0
57	1647629.0
58	1375635.5
59	1112884.0
60	921344.5
61	818138.5
62	763769.5
63	710516.5
64	604227.5
65	500519.5
66	440787.0
67	394565.0
68	326432.0
69	253384.0
70	188949.5
71	138880.5
72	105046.5
73	82918.5
74	71285.0
75	53486.5
76	33300.0
77	25673.5
78	20072.0
79	15957.0
80	13675.0
81	10141.0
82	6520.0
83	4214.0
84	3620.5
85	3139.0
86	2782.0
87	2422.5
88	2067.5
89	1768.5
90	1498.0
91	1315.0
92	1156.5
93	998.5
94	949.5
95	916.0
96	920.0
97	1012.0
98	1170.5
99	1568.0
100	8643.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07700387963052832
2	0.04619821922909309
3	0.031066674116835925
4	0.030942209243288374
5	0.03090474894154105
6	0.03179050381834064
7	0.030360970367789595
8	0.030978461148205138
9	0.03035251158997568
10-11	0.030378492121832697
12-13	0.030371241740849344
14-15	0.030371845939264622
16-17	0.03116032487120423
18-19	0.03042501539980921
20-21	0.030399034867952195
22-23	0.03247566482126748
24-25	0.03469851079108037
26-27	0.03773339943102877
28-29	0.04073143196764512
30-31	0.043270273708649144
32-33	0.045945060093091024
34-35	0.04803437821312717
36-37	1.922305884977461E-4
38-39	5.192744834745278E-4
40-41	6.728343145500421E-4
42-43	0.0011014644974022638
44-45	8.892919862253688E-4
46-47	6.245132174897387E-4
48-49	5.912758624923776E-4
50-51	0.0010102693852784065
52-53	3.7908604651676E-4
54-55	3.567242395203019E-5
56-57	7.55790269482039E-5
58-59	2.0557986688643157E-5
60-61	3.688437893039715E-5
62-63	3.0233884858651567E-6
64-65	2.055960101651263E-5
66-67	9.070679441722902E-6
68-69	2.056082206230391E-5
70-71	2.4189941159661427E-5
72-73	0.0
74-75	1.0886201779160987E-5
76-77	3.5078955077037346E-5
78-79	5.141075776517461E-5
80-81	2.6008854115575146E-4
82-83	0.0015587831931524305
84-85	1.5304216921510914E-4
86-87	0.0012606945569203984
88-89	5.535497091994662E-4
90-91	0.002234326224225182
92-93	0.0010359647382291986
94-95	0.0010941831920353291
96-97	0.0018381847313857138
98-99	0.0016554383346477715
100-101	3.3600081854134894E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	40763.0
36-37	1571.0
38-39	1699.0
40-41	1721.0
42-43	1831.0
44-45	1829.0
46-47	1858.0
48-49	1820.0
50-51	1811.0
52-53	1967.0
54-55	2034.0
56-57	1936.0
58-59	2053.0
60-61	2159.0
62-63	2183.0
64-65	2412.0
66-67	2489.0
68-69	2508.0
70-71	2705.0
72-73	2762.0
74-75	2827.0
76-77	2979.0
78-79	3347.0
80-81	3493.0
82-83	3593.0
84-85	4053.0
86-87	4289.0
88-89	5048.0
90-91	13471.0
92-93	9046.0
94-95	29096.0
96-97	585637.0
98-99	7468434.0
100-101	7.4538848E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.983692490232475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.794704728638806	14.820651957363875
2	15.208683199186634	7.295207620613146
3	7.098087337670858	5.107150319265323
4	3.962172013576961	3.801100606681402
5	2.4525250543975123	2.9410303364630304
6	1.7234293840812567	2.480052022586137
7	1.2229108479297808	2.0530942403802115
8	0.9149776601266757	1.7555634268728508
9	0.6956146171938921	1.501506636344018
>10	4.257018218332543	18.869760566113502
>50	0.359952254979546	5.964271969374182
>100	0.2526456348468355	12.055279928767119
>500	0.03024121193635063	5.001738420462763
>1k	0.024744997945725516	11.621910206226778
>5k	0.001867188323060558	3.033668130932662
>10k+	4.2565083352444333E-4	1.6980136115528919
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.833587322235159E-6	0.0	1.2083968305587898E-6	0.0	7.250380983352739E-6
2	6.0419841527939486E-6	2.4167936611175797E-6	4.833587322235159E-6	2.4167936611175797E-6	1.0875571475029108E-5
3	1.0875571475029108E-5	2.4167936611175797E-6	4.833587322235159E-6	2.4167936611175797E-6	1.5709158797264266E-5
4	1.6917555627823056E-5	4.833587322235159E-6	1.2083968305587897E-5	2.4167936611175797E-6	1.8125952458381845E-5
5	2.0542746119499427E-5	4.833587322235159E-6	2.4167936611175794E-5	3.6251904916763693E-6	1.9334349288940637E-5
6	3.0209920763969744E-5	6.0419841527939486E-6	3.383511125564611E-5	3.6251904916763693E-6	1.9334349288940637E-5
7	3.141831759452853E-5	6.0419841527939486E-6	4.350228590011643E-5	4.833587322235159E-6	2.1751142950058216E-5
8	4.471068273067522E-5	6.0419841527939486E-6	4.350228590011643E-5	4.833587322235159E-6	2.1751142950058216E-5
9	4.954427005291038E-5	7.250380983352739E-6	4.471068273067522E-5	9.667174644470319E-6	2.4167936611175794E-5
10-11	6.827442092657162E-5	7.250380983352739E-6	5.196106371402796E-5	9.667174644470319E-6	3.141831759452853E-5
12-13	8.458777813911529E-5	8.458777813911528E-6	6.767022251129222E-5	9.667174644470319E-6	4.168969065427825E-5
14-15	1.1419350048780564E-4	8.458777813911528E-6	9.183815912246802E-5	1.3292365136146688E-5	4.8335873222351595E-5
16-17	1.329236513614669E-4	1.0875571475029108E-5	1.1298510365724684E-4	1.8125952458381845E-5	5.196106371402796E-5
18-19	1.3836143709898143E-4	1.2083968305587897E-5	1.1963128622532019E-4	1.8125952458381845E-5	5.6190452620983725E-5
20-21	1.4682021491289296E-4	1.2083968305587897E-5	1.3654884185314325E-4	1.873015087366124E-5	6.223243677377766E-5
22-23	1.5588319114208388E-4	1.2083968305587897E-5	1.5588319114208388E-4	1.9334349288940637E-5	6.767022251129222E-5
24-25	1.752175404310245E-4	1.2083968305587897E-5	1.6132097687959843E-4	1.9334349288940637E-5	6.887861934185101E-5
26-27	2.108652469325088E-4	1.2083968305587897E-5	1.7159234993934815E-4	2.4772135026455192E-5	6.887861934185101E-5
28-29	2.664515011382132E-4	1.2083968305587897E-5	1.7944692933798029E-4	2.6584730272293376E-5	7.492060349464497E-5
30-31	3.07536993377212E-4	1.2083968305587897E-5	1.800511277532597E-4	2.7793127102852165E-5	7.914999240160074E-5
32-33	3.5587286659956356E-4	1.2083968305587897E-5	1.8246792141437727E-4	2.7793127102852165E-5	8.21709844779977E-5
34-35	4.036045414066358E-4	1.2083968305587897E-5	2.1690723108530276E-4	2.960572234869035E-5	8.640037338495348E-5
36-37	4.591907956123401E-4	1.2688166720867293E-5	2.2899119939089066E-4	3.0209920763969744E-5	9.062976229190923E-5
38-39	5.298820102000294E-4	1.3292365136146688E-5	2.362415803742434E-4	3.081411917924914E-5	1.0694311950445289E-4
40-41	6.060110105252331E-4	1.9334349288940637E-5	2.5013814392566946E-4	3.141831759452853E-5	1.2083968305587897E-4
42-43	6.924113839101865E-4	2.2959539780617005E-5	2.628263106465368E-4	3.141831759452853E-5	1.280900640392317E-4
44-45	7.776033604645812E-4	2.35637381958964E-5	2.664515011382132E-4	3.323091284036672E-5	1.413824291753784E-4
46-47	8.585659481120201E-4	2.4167936611175794E-5	2.7732707261324225E-4	3.383511125564611E-5	1.4621601649761356E-4
48-49	9.600712818789584E-4	2.5376333441734584E-5	2.839732551813156E-4	3.383511125564611E-5	1.5527899272680447E-4
50-51	0.0010519094410014265	2.5376333441734584E-5	2.8820264408827137E-4	3.383511125564611E-5	1.6252937371015724E-4
52-53	0.0011582483620905999	2.5376333441734584E-5	3.0209920763969744E-4	3.5647706501484295E-5	1.6434196895599539E-4
54-55	0.0012573369021964208	2.5376333441734584E-5	3.1478737436056476E-4	3.746030174732248E-5	1.7159234993934815E-4
56-57	0.001354008648641124	2.7793127102852165E-5	3.329133268189466E-4	3.9877095408440064E-5	1.9032250081300937E-4
58-59	0.0014530971887469447	2.7793127102852165E-5	3.3835111255646114E-4	4.048129382371946E-5	2.0724005644083245E-4
60-61	0.0015558109193444416	2.7793127102852165E-5	3.7097782698154844E-4	4.591907956123401E-5	2.0905265168667063E-4
62-63	0.0017008185390114967	3.081411917924914E-5	4.138759144663855E-4	4.833587322235159E-5	2.12677842178347E-4
64-65	0.0018548891349077423	3.262671442508732E-5	4.4348163681507584E-4	4.833587322235159E-5	2.2174081840753792E-4
66-67	0.001990833778345606	3.262671442508732E-5	4.4469003364563464E-4	4.833587322235159E-5	2.2778280256033188E-4
68-69	0.00215638414413216	3.262671442508732E-5	4.48315224137311E-4	4.833587322235159E-5	2.338247867131258E-4
70-71	0.00229655817647698	3.262671442508732E-5	4.5012781938314915E-4	5.014846846818977E-5	2.4107516769647855E-4
72-73	0.0024306902246690057	3.262671442508732E-5	4.670453750109722E-4	5.075266688346917E-5	2.5315913600206647E-4
74-75	0.002603490971438913	3.262671442508732E-5	4.736915575790456E-4	5.075266688346917E-5	2.628263106465368E-4
76-77	0.0027666245435643494	3.262671442508732E-5	4.77316748070722E-4	5.075266688346917E-5	2.676598979687719E-4
78-79	0.00287538025831464	3.625190491676369E-5	4.7973354173183955E-4	5.316946054458675E-5	2.755144773674041E-4
80-81	0.0029666142190218287	3.8668698577881275E-5	4.833587322235159E-4	5.679465103626312E-5	2.797438662743598E-4
82-83	0.003036097036778959	3.9877095408440064E-5	4.857755258846335E-4	5.679465103626312E-5	2.851816520118744E-4
84-85	0.0031146428307652803	4.108549223899885E-5	4.906091132068687E-4	5.679465103626312E-5	2.8880684250355077E-4
86-87	0.0031527073309278826	4.108549223899885E-5	5.020888830971771E-4	5.7398849451542514E-5	2.9726562031746233E-4
88-89	0.003161166108741794	4.350228590011643E-5	5.045056767582947E-4	5.800304786682191E-5	3.0632859654665325E-4
90	0.003161166108741794	4.471068273067522E-5	5.063182720041329E-4	5.800304786682191E-5	3.081411917924914E-4
>>END_MODULE
