##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290295_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88251117
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01316826392124	37.0	37.0	37.0	37.0	37.0
2	36.1612577776211	37.0	37.0	37.0	37.0	37.0
3	36.288920886973024	37.0	37.0	37.0	37.0	37.0
4	36.32388444443145	37.0	37.0	37.0	37.0	37.0
5	36.34405711827987	37.0	37.0	37.0	37.0	37.0
6	36.308064316058456	37.0	37.0	37.0	37.0	37.0
7	36.27375535654693	37.0	37.0	37.0	37.0	37.0
8	36.330726261515764	37.0	37.0	37.0	37.0	37.0
9	36.3147910637777	37.0	37.0	37.0	37.0	37.0
10-11	36.33512272711517	37.0	37.0	37.0	37.0	37.0
12-13	36.31843717626826	37.0	37.0	37.0	37.0	37.0
14-15	36.3133738862478	37.0	37.0	37.0	37.0	37.0
16-17	36.313335280504155	37.0	37.0	37.0	37.0	37.0
18-19	36.308290539823986	37.0	37.0	37.0	37.0	37.0
20-21	36.302501734907224	37.0	37.0	37.0	37.0	37.0
22-23	36.287663084196424	37.0	37.0	37.0	37.0	37.0
24-25	36.2562772434937	37.0	37.0	37.0	37.0	37.0
26-27	36.208883832031276	37.0	37.0	37.0	37.0	37.0
28-29	36.195148283505574	37.0	37.0	37.0	37.0	37.0
30-31	36.18545542602027	37.0	37.0	37.0	37.0	37.0
32-33	36.179409236259296	37.0	37.0	37.0	37.0	37.0
34-35	36.16574839500332	37.0	37.0	37.0	37.0	37.0
36-37	36.163823821446314	37.0	37.0	37.0	37.0	37.0
38-39	36.155358567685184	37.0	37.0	37.0	37.0	37.0
40-41	36.155929762392844	37.0	37.0	37.0	37.0	37.0
42-43	36.13898727529442	37.0	37.0	37.0	37.0	37.0
44-45	36.13755287120514	37.0	37.0	37.0	37.0	37.0
46-47	36.13284562067446	37.0	37.0	37.0	37.0	37.0
48-49	36.104530421750425	37.0	37.0	37.0	37.0	37.0
50-51	36.09828665079284	37.0	37.0	37.0	37.0	37.0
52-53	36.0980725287254	37.0	37.0	37.0	37.0	37.0
54-55	36.08811370814161	37.0	37.0	37.0	37.0	37.0
56-57	36.07254263579208	37.0	37.0	37.0	37.0	37.0
58-59	36.06090927183655	37.0	37.0	37.0	37.0	37.0
60-61	36.057374010043326	37.0	37.0	37.0	37.0	37.0
62-63	36.045567063289084	37.0	37.0	37.0	37.0	37.0
64-65	36.03044324851652	37.0	37.0	37.0	37.0	37.0
66-67	36.02671372478005	37.0	37.0	37.0	37.0	37.0
68-69	36.01004815148447	37.0	37.0	37.0	37.0	37.0
70-71	35.987832669149384	37.0	37.0	37.0	37.0	37.0
72-73	35.96508846229338	37.0	37.0	37.0	37.0	37.0
74-75	35.96024394543395	37.0	37.0	37.0	37.0	37.0
76-77	35.94678709244569	37.0	37.0	37.0	37.0	37.0
78-79	35.933606138698224	37.0	37.0	37.0	37.0	37.0
80-81	35.905060460437255	37.0	37.0	37.0	37.0	37.0
82-83	35.91812642542107	37.0	37.0	37.0	37.0	37.0
84-85	35.90042429065461	37.0	37.0	37.0	37.0	37.0
86-87	35.91346571772591	37.0	37.0	37.0	37.0	37.0
88-89	35.887014943978656	37.0	37.0	37.0	37.0	37.0
90-91	35.86924225651032	37.0	37.0	37.0	37.0	37.0
92-93	35.856908805721034	37.0	37.0	37.0	37.0	37.0
94-95	35.827971358776125	37.0	37.0	37.0	37.0	37.0
96-97	35.82713001841972	37.0	37.0	37.0	37.0	37.0
98-99	35.80480549941386	37.0	37.0	37.0	37.0	37.0
100-101	35.7770602367342	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27427.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	14.0
15	112.0
16	577.0
17	1630.0
18	2888.0
19	4513.0
20	6176.0
21	10253.0
22	17990.0
23	35529.0
24	65837.0
25	114471.0
26	177867.0
27	276630.0
28	410015.0
29	599855.0
30	817472.0
31	1120254.0
32	1483445.0
33	2138684.0
34	3406150.0
35	7956300.0
36	5.2503248E7
37	1.7073779E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.005416578790413	16.796798356317225	11.216368340964394	46.981416723927964
2	21.36834754661881	19.14331758083088	33.44857443198824	26.039760440562066
3	22.862302630246333	19.595224508578305	23.522154741353127	34.020318119822235
4	24.04446356755198	24.743055975101473	21.369024578319042	29.843455879027502
5	25.624570813841636	28.712151750035588	23.375119892720843	22.288157543401933
6	21.57346739860915	33.26203880159626	23.40446313229474	21.76003066749985
7	18.789504270338274	22.935400911025145	37.63783174337868	20.637263075257902
8	19.27625901841104	24.59414359113748	30.258115478960356	25.871481911491117
9	20.247110727822772	22.71006260008012	32.19990948236136	24.84291718973574
10-11	22.209222375532015	30.793218918864085	24.533274452700855	22.464284252903045
12-13	21.766094115278083	26.269643007076304	27.499123747037608	24.46513913060801
14-15	21.85393096905575	26.69654744398717	27.035121217015284	24.414400369941795
16-17	22.65509269225771	26.667468189905396	26.406566028196206	24.270873089640684
18-19	22.4782180945648	26.959443448605757	26.07270139980651	24.489637057022932
20-21	22.630433061932603	26.800224221023335	26.312580106767953	24.256762610276112
22-23	22.434518576132632	26.858534933730443	26.457717022102127	24.249229468034795
24-25	22.311637935641883	26.764690316346023	26.572105038283834	24.35156670972826
26-27	22.416958479255214	26.911501562642375	26.51981042153758	24.15172953656483
28-29	22.34096519304406	26.839984394095147	26.43990935951527	24.379141053345528
30-31	22.33300303367411	26.613890554626657	26.528291814939557	24.524814596759676
32-33	22.59540289807824	26.73184701608662	26.379199246907838	24.293550838927306
34-35	22.61921262196982	26.75310111938997	26.210106220767475	24.417580037872728
36-37	22.357372535161637	26.66591642538481	26.46850165375146	24.508209385702095
38-39	22.614657824189734	26.81637924353867	26.49129417077965	24.077668761491946
40-41	22.740385527828348	26.710603639930923	26.22867174653825	24.32033908570248
42-43	22.582791316631642	26.62603975940994	26.440028755380162	24.351140168578254
44-45	22.66986518667967	26.641115150830473	26.501435523886357	24.187584138603498
46-47	22.627404916962014	26.61222024402945	26.369090142791794	24.391284696216744
48-49	22.5098391708743	26.42761087784569	26.50046225529276	24.562087695987245
50-51	22.74424663482414	26.44432572805772	26.39695426136552	24.414473375752614
52-53	22.9619361678054	26.60210749595849	26.126666275358417	24.309290060877686
54-55	22.646331085582496	26.518609534884728	26.384534830159463	24.450524549373313
56-57	22.702007017109533	26.675119748576115	26.376957880565964	24.245915353748387
58-59	22.723903516117936	26.554476555324207	26.327251212979412	24.394368715578445
60-61	22.712997988283057	26.418366985849122	26.331983764898748	24.536651260969077
62-63	22.80848487337189	26.39440658836466	26.401828885854687	24.395279652408764
64-65	22.888669559456307	26.544241944633267	26.140167157606232	24.426921338304194
66-67	22.88615118345478	26.476195223221072	26.214618931936506	24.423034661387643
68-69	22.92259809986969	26.59538021319476	26.295658303174786	24.18636338376077
70-71	23.170593158598727	26.538468627032785	26.11005919864326	24.18087901572523
72-73	22.957354284721593	26.455466783003196	26.192194286935795	24.394984645339413
74-75	23.002211099802675	26.52803170717571	26.23200021959009	24.237756973431527
76-77	23.063215664410812	26.480725284377176	26.14560326110006	24.310455790111956
78-79	22.841829730280676	26.50483687936714	26.209133632909932	24.444199757442256
80-81	23.03356032965969	26.554935082708642	26.170199164332914	24.241305423298755
82-83	23.080568193222348	26.574235959781173	25.98424515750523	24.360950689491247
84-85	23.019137201372622	26.519700013522918	26.0746376719521	24.38652511315236
86-87	23.049675064827806	26.547619543299906	26.075055565513562	24.327649826358726
88-89	23.175462588894863	26.38950204036768	26.025443231650673	24.409592139086786
90-91	23.001779117233063	26.36765637579945	26.263575929872307	24.366988577095178
92-93	23.193824999375114	26.350376947962623	26.237736718301573	24.21806133436069
94-95	23.24297821758669	26.36573239826001	26.105173019900107	24.28611636425319
96-97	23.105805659861506	26.232855489410795	26.203821037099228	24.457517813628474
98-99	23.31373510798545	25.428692330312426	26.648645653353846	24.608926908348273
100-101	24.080936694347756	11.991681395671682	32.82536537344345	31.102016536537114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27560.0
1	13812.0
2	75.0
3	90.0
4	108.5
5	143.0
6	156.0
7	160.0
8	169.0
9	184.0
10	250.5
11	374.5
12	520.5
13	747.0
14	1081.0
15	1565.0
16	2439.5
17	3515.5
18	4788.5
19	6555.0
20	8759.0
21	11732.0
22	16226.5
23	22790.0
24	31456.0
25	42468.0
26	56596.0
27	77384.0
28	106272.5
29	141893.5
30	191056.5
31	256713.0
32	355081.5
33	487508.5
34	644655.5
35	847280.5
36	1121035.5
37	1464139.0
38	1892210.5
39	2414896.5
40	2950622.0
41	3474594.0
42	4026894.5
43	4501773.0
44	4867570.0
45	5148488.0
46	5271414.0
47	5245594.5
48	5062276.0
49	4776486.0
50	4431381.0
51	4064066.0
52	3640088.5
53	3122767.5
54	2653021.0
55	2308500.0
56	2001045.5
57	1679270.5
58	1390299.5
59	1133100.5
60	947236.0
61	832781.0
62	755053.0
63	683707.0
64	597951.5
65	518980.5
66	460426.0
67	400620.5
68	318981.5
69	240153.5
70	180970.0
71	134546.5
72	100469.5
73	76180.0
74	60710.5
75	42718.5
76	24656.5
77	16753.0
78	11135.5
79	7060.5
80	5558.0
81	3877.0
82	1842.5
83	609.0
84	452.5
85	365.0
86	282.0
87	220.0
88	207.0
89	176.5
90	149.0
91	133.0
92	125.0
93	124.5
94	145.5
95	210.0
96	434.0
97	1207.5
98	3490.0
99	4085.0
100	1599.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06676062808360828
2	0.041189280357777226
3	0.03294689176568723
4	0.03107836017531653
5	0.031108954688924786
6	0.03107836017531653
7	0.03107836017531653
8	0.03107836017531653
9	0.031079493305450175
10-11	0.03107836017531653
12-13	0.031103855603323412
14-15	0.03117071028120811
16-17	0.03108459239105155
18-19	0.03127722451377018
20-21	0.031108954688924786
22-23	0.031884582265400675
24-25	0.03510210527986858
26-27	0.03784144737794084
28-29	0.040554727482939396
30-31	0.042815888664616
32-33	0.045176198732986006
34-35	0.04759259874297115
36-37	1.2527309392764582E-4
38-39	1.9840022754125298E-5
40-41	2.6076043524998112E-5
42-43	3.594041127428933E-4
44-45	9.013665431083992E-5
46-47	1.7574218390322007E-4
48-49	1.4569914107011496E-4
50-51	6.916613735673737E-5
52-53	1.0431851968210677E-4
54-55	3.4584695299422834E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	8.505305796872817E-6
64-65	3.969250173542705E-6
66-67	6.8046167759763135E-6
68-69	0.0
70-71	5.670856963061813E-7
72-73	7.372355239863491E-6
74-75	0.0
76-77	1.7014292771571294E-6
78-79	0.0
80-81	1.7015606657707761E-6
82-83	5.16161779713111E-5
84-85	9.643021197794038E-6
86-87	7.374434022824951E-6
88-89	2.705990414360824E-4
90-91	4.935812229856827E-5
92-93	6.809210026264429E-6
94-95	5.765835428608229E-4
96-97	2.897535689643066E-5
98-99	1.9656129996255103E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	43430.0
36-37	1698.0
38-39	1937.0
40-41	1947.0
42-43	2173.0
44-45	2084.0
46-47	1894.0
48-49	1980.0
50-51	1997.0
52-53	2177.0
54-55	2108.0
56-57	2212.0
58-59	2264.0
60-61	2354.0
62-63	2378.0
64-65	2457.0
66-67	2495.0
68-69	2691.0
70-71	2895.0
72-73	2970.0
74-75	3048.0
76-77	3088.0
78-79	3531.0
80-81	3707.0
82-83	3891.0
84-85	4257.0
86-87	4445.0
88-89	5271.0
90-91	14789.0
92-93	9843.0
94-95	33745.0
96-97	629082.0
98-99	7954611.0
100-101	7.9491668E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.202964680146955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.04030565945785	12.074893741604523
2	17.930797123215072	8.320953046736799
3	9.134977479290228	6.358756794177271
4	5.4472557109061	5.055699274555322
5	3.331427965939357	3.8649502714072357
6	2.2759579890643558	3.1685383700255123
7	1.6024512220209854	2.602713337434788
8	1.188371657884211	2.205899648382003
9	0.9160840464378678	1.9130279196189555
>10	5.379183900229579	23.026241438663934
>50	0.4298989670908751	6.90808610404633
>100	0.28172367313127106	12.690348028534732
>500	0.026025859373954154	4.136658275734836
>1k	0.014812771801555467	6.255852763018861
>5k	5.584416592847767E-4	0.8115737075490655
>10k+	1.6753249778543304E-4	0.6058072785094953
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1331301336389885E-6	0.0	1.1331301336389885E-6	2.266260267277977E-6	4.532520534555954E-6
2	3.399390400916965E-6	0.0	7.93191093547292E-6	3.399390400916965E-6	4.532520534555954E-6
3	6.79878080183393E-6	0.0	1.1331301336389884E-5	3.399390400916965E-6	1.0198171202750896E-5
4	1.1331301336389884E-5	0.0	1.1331301336389884E-5	3.399390400916965E-6	1.359756160366786E-5
5	2.266260267277977E-5	0.0	1.6996952004584825E-5	3.399390400916965E-6	1.6996952004584825E-5
6	4.1925814944642574E-5	0.0	2.4928862940057745E-5	4.532520534555954E-6	1.6996952004584825E-5
7	4.1925814944642574E-5	0.0	3.512703414280864E-5	4.532520534555954E-6	1.9263212271862802E-5
8	4.985772588011549E-5	0.0	3.512703414280864E-5	5.665650668194942E-6	2.0396342405501792E-5
9	5.099085601375448E-5	0.0	4.1925814944642574E-5	9.065041069111908E-6	2.4928862940057745E-5
10-11	6.402185255060284E-5	3.399390400916965E-6	5.325711628103246E-5	1.0198171202750896E-5	3.3427338942350156E-5
12-13	7.195376348607576E-5	5.665650668194942E-6	7.308689361971475E-5	1.0198171202750896E-5	5.608994161512993E-5
14-15	8.668445522338262E-5	5.665650668194942E-6	9.80157565597725E-5	1.359756160366786E-5	6.96875032187978E-5
16-17	9.80157565597725E-5	6.232215735014436E-6	1.2181148936619125E-4	1.6996952004584825E-5	7.931910935472919E-5
18-19	1.0991362296298188E-4	6.79878080183393E-6	1.3370935576940062E-4	1.6996952004584825E-5	9.178354082475806E-5
20-21	1.286102701680252E-4	7.93191093547292E-6	1.5467226324172192E-4	1.8130082138223812E-5	1.0198171202750896E-4
22-23	1.4334096190533202E-4	7.93191093547292E-6	1.8016769124859914E-4	2.0396342405501792E-5	1.1104675309662087E-4
24-25	1.648704344444728E-4	7.93191093547292E-6	1.8300051658269663E-4	2.2662602672779765E-5	1.1217988323025986E-4
26-27	1.852667768499746E-4	9.065041069111908E-6	1.8753303711725259E-4	2.436229787323825E-5	1.2011179416573277E-4
28-29	2.0396342405501792E-4	1.6996952004584825E-5	2.0113059872092044E-4	2.4928862940057745E-5	1.2747714003438619E-4
30-31	2.3115854726235362E-4	1.6996952004584825E-5	2.158612904582273E-4	2.4928862940057745E-5	1.4220783177169304E-4
32-33	2.5835367046968933E-4	1.8130082138223812E-5	2.232266363268807E-4	2.662855814051623E-5	1.529725680412634E-4
34-35	2.787500128751912E-4	2.0962907472321287E-5	2.640193211378843E-4	2.9461383474613698E-5	1.6147104404355586E-4
36-37	2.9801322514705396E-4	2.266260267277977E-5	2.725177971401767E-4	2.9461383474613698E-5	1.665701296449313E-4
38-39	3.45038125693072E-4	2.3795732806418755E-5	2.8781505394430304E-4	3.172764374189168E-5	1.8583334191679408E-4
40-41	3.784654646354221E-4	2.719512320733572E-5	3.121773518175413E-4	3.399390400916965E-5	1.9036586245135004E-4
42-43	4.090599782436748E-4	3.059451360825269E-5	3.342733894235016E-4	3.399390400916965E-5	1.96031513119545E-4
44-45	4.37954796651469E-4	3.059451360825269E-5	3.4560469075989146E-4	3.5693599209628136E-5	2.124619000573103E-4
46-47	4.736483958610972E-4	3.059451360825269E-5	3.6883385849949073E-4	3.6826729343267126E-5	2.141615952577688E-4
48-49	5.201067313402956E-4	3.172764374189168E-5	3.773323345017831E-4	4.305894507828156E-5	2.243597664605197E-4
50-51	5.659985017526747E-4	3.286077387553067E-5	3.8866363583817305E-4	4.305894507828156E-5	2.368241979305486E-4
52-53	6.096240118977757E-4	3.399390400916965E-5	4.101931083773138E-4	4.419207521192055E-5	2.4588923899966045E-4
54-55	6.674136487133642E-4	3.7393294410086614E-5	4.37954796651469E-4	4.532520534555954E-5	2.4872206433375793E-4
56-57	7.082063335243677E-4	3.8526424543725604E-5	4.6628304999244373E-4	4.759146561283751E-5	2.623196259374258E-4
58-59	7.682622306072341E-4	3.9659554677364594E-5	4.7988061159611156E-4	4.985772588011549E-5	2.8214940327610813E-4
60-61	8.543801207637973E-4	4.0792684811003584E-5	5.161407758725591E-4	5.099085601375448E-5	2.844156635433861E-4
62-63	9.053709767775517E-4	4.0792684811003584E-5	5.665650668194942E-4	5.325711628103245E-5	2.8781505394430304E-4
64-65	9.535290074572087E-4	4.0792684811003584E-5	5.914939297595519E-4	5.495681148149094E-5	2.9574696487977596E-4
66-67	0.001009052384005519	4.0792684811003584E-5	5.92627059893191E-4	5.552337654831043E-5	3.0934452648344383E-4
68-69	0.0010583435448188153	4.0792684811003584E-5	6.005589708286638E-4	5.552337654831043E-5	3.1897613261937523E-4
70-71	0.0011099009658993892	4.0792684811003584E-5	6.005589708286638E-4	5.608994161512993E-5	3.3653964969077954E-4
72-73	0.0011654243424476995	4.0792684811003584E-5	6.152896625659707E-4	5.7789636815588406E-5	3.6316820783129577E-4
74-75	0.001234545280599678	4.0792684811003584E-5	6.152896625659707E-4	6.0055897082866386E-5	3.7393294410086617E-4
76-77	0.0013025330886180173	5.042429094693498E-5	6.164227926996097E-4	6.0055897082866386E-5	3.835645502367976E-4
78-79	0.0013501245542308546	5.325711628103245E-5	6.209553132341657E-4	6.0055897082866386E-5	3.988618070409239E-4
80-81	0.0013999822801109701	5.948933201604689E-5	6.26054398835541E-4	6.0055897082866386E-5	4.0452745770911883E-4
82-83	0.0014345427491869595	6.118902721650538E-5	6.322866145705556E-4	6.0055897082866386E-5	4.209578446468842E-4
84-85	0.001483267344933436	6.458841761742233E-5	6.345528748378335E-4	6.0055897082866386E-5	4.328557110500936E-4
86-87	0.0014996977318712012	6.96875032187978E-5	6.396519604392089E-4	6.0055897082866386E-5	4.4022105691874703E-4
88-89	0.0015025305572052986	7.082063335243677E-5	6.441844809737648E-4	6.175559228332488E-5	4.594842691906098E-4
90	0.0015025305572052986	7.138719841925627E-5	6.458841761742234E-4	6.345528748378336E-5	4.657164849256242E-4
>>END_MODULE
