##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290292_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83758105
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.112306611998925	37.0	37.0	37.0	37.0	37.0
2	36.116838889800576	37.0	37.0	37.0	37.0	37.0
3	36.21538202183538	37.0	37.0	37.0	37.0	37.0
4	36.19111195268804	37.0	37.0	37.0	37.0	37.0
5	36.152380763628784	37.0	37.0	37.0	37.0	37.0
6	36.070278165916	37.0	37.0	37.0	37.0	37.0
7	36.03423374967712	37.0	37.0	37.0	37.0	37.0
8	36.11252310448046	37.0	37.0	37.0	37.0	37.0
9	36.05986350813453	37.0	37.0	37.0	37.0	37.0
10-11	36.09096407446181	37.0	37.0	37.0	37.0	37.0
12-13	36.07326092800213	37.0	37.0	37.0	37.0	37.0
14-15	36.06456670073899	37.0	37.0	37.0	37.0	37.0
16-17	36.045924325771225	37.0	37.0	37.0	37.0	37.0
18-19	36.053436625625665	37.0	37.0	37.0	37.0	37.0
20-21	36.04371513061333	37.0	37.0	37.0	37.0	37.0
22-23	36.01337113584411	37.0	37.0	37.0	37.0	37.0
24-25	36.00042502155463	37.0	37.0	37.0	37.0	37.0
26-27	35.97080137498335	37.0	37.0	37.0	37.0	37.0
28-29	35.95339019429821	37.0	37.0	37.0	37.0	37.0
30-31	35.93233221429735	37.0	37.0	37.0	37.0	37.0
32-33	35.908969048428204	37.0	37.0	37.0	37.0	37.0
34-35	35.89102902340018	37.0	37.0	37.0	37.0	37.0
36-37	35.88478906789515	37.0	37.0	37.0	37.0	37.0
38-39	35.87140750968233	37.0	37.0	37.0	37.0	37.0
40-41	35.87473745049574	37.0	37.0	37.0	37.0	37.0
42-43	35.834976857058585	37.0	37.0	37.0	37.0	37.0
44-45	35.821553047245956	37.0	37.0	37.0	37.0	37.0
46-47	35.82531042533597	37.0	37.0	37.0	37.0	37.0
48-49	35.80107130733629	37.0	37.0	37.0	37.0	37.0
50-51	35.7810783629063	37.0	37.0	37.0	37.0	37.0
52-53	35.76731726711116	37.0	37.0	37.0	37.0	37.0
54-55	35.76144659537079	37.0	37.0	37.0	37.0	37.0
56-57	35.72694719939168	37.0	37.0	37.0	37.0	37.0
58-59	35.75126481780283	37.0	37.0	37.0	37.0	37.0
60-61	35.715687818092874	37.0	37.0	37.0	37.0	37.0
62-63	35.702477723844964	37.0	37.0	37.0	37.0	37.0
64-65	35.68793822343153	37.0	37.0	37.0	37.0	37.0
66-67	35.67008039220665	37.0	37.0	37.0	37.0	37.0
68-69	35.63495221468452	37.0	37.0	37.0	37.0	37.0
70-71	35.64146032604445	37.0	37.0	37.0	37.0	37.0
72-73	35.61758430075864	37.0	37.0	37.0	37.0	37.0
74-75	35.62894064427674	37.0	37.0	37.0	37.0	37.0
76-77	35.60148423307495	37.0	37.0	37.0	37.0	37.0
78-79	35.5557444646147	37.0	37.0	37.0	37.0	37.0
80-81	35.54971757286876	37.0	37.0	37.0	37.0	37.0
82-83	35.51654007007109	37.0	37.0	37.0	37.0	37.0
84-85	35.47113681684385	37.0	37.0	37.0	37.0	37.0
86-87	35.43143091786398	37.0	37.0	37.0	37.0	37.0
88-89	35.461142451511094	37.0	37.0	37.0	37.0	37.0
90-91	35.39885376458136	37.0	37.0	37.0	37.0	37.0
92-93	35.444870984660575	37.0	37.0	37.0	37.0	37.0
94-95	35.39581505648661	37.0	37.0	37.0	37.0	37.0
96-97	35.36965665033858	37.0	37.0	37.0	37.0	37.0
98-99	35.36481585984306	37.0	37.0	37.0	37.0	37.0
100-101	35.34668321384122	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26977.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	31.0
12	1277.0
13	8932.0
14	17960.0
15	19961.0
16	19737.0
17	22773.0
18	26090.0
19	29100.0
20	35956.0
21	53444.0
22	78985.0
23	116336.0
24	154524.0
25	197818.0
26	244333.0
27	320136.0
28	412673.0
29	556176.0
30	744455.0
31	1065605.0
32	1563778.0
33	2604552.0
34	4855775.0
35	1.2560026E7
36	4.8864592E7
37	9156103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.188052553976885	15.844490720116768	11.824704542526879	47.142752183379464
2	21.76576908535461	19.580527869146497	33.04607630467612	25.60762674082277
3	23.5944685964023	20.209742681346874	23.229509599739412	32.96627912251141
4	25.14242971759606	25.329661159101995	20.683984151790632	28.84392497151131
5	26.11851777256381	29.294969372738045	23.114977801672392	21.471535053025757
6	21.69450398441752	33.556200273904444	23.129794805752258	21.619500935925778
7	19.0892458882491	21.626755300221838	38.20558922908446	21.078409582444603
8	19.9062459438348	23.80147708069899	29.63536827483928	26.65690870062693
9	20.970983956432164	22.027865718657598	31.66036486837643	25.34078545653381
10-11	22.90754666039755	30.398057667858204	23.883608761769885	22.81078690997436
12-13	22.102312591419498	25.672881459360543	27.528741213473563	24.6960647357464
14-15	22.181525747892987	26.287560955462514	26.94217245779253	24.588740838851976
16-17	23.12796689197849	26.181655747066834	26.319085515932095	24.37129184502258
18-19	22.93497278211644	26.57427778346961	25.853544125576946	24.637205308837
20-21	22.964177415399107	26.316925504162736	26.220855436412677	24.49804164402548
22-23	22.828185923620246	26.421649895052578	26.38458033291687	24.365583848410306
24-25	22.76883313744638	26.272364059219132	26.48446683693938	24.47433596639511
26-27	22.838921842151482	26.53202263624411	26.333255372857415	24.295800148746988
28-29	22.702215453216482	26.387774247155477	26.331097029232765	24.578913270395276
30-31	22.785062303886676	26.14122799605005	26.39695915052089	24.676750549542387
32-33	22.998089033418868	26.303605273971048	26.284319895607393	24.413985797002695
34-35	23.038396679761632	26.28090154875162	26.155791397975044	24.524910373511705
36-37	22.876086930942378	26.194459481082777	26.333007395159093	24.596446192815755
38-39	23.13108278714348	26.342614312519043	26.377319915036264	24.148982985301217
40-41	23.247588842560628	26.19088795662121	26.032811057278533	24.528712143539636
42-43	23.011912566701355	26.26102789940021	26.277385417301463	24.449674116596967
44-45	23.141430754363135	26.218621023964346	26.39261883691315	24.24732938475937
46-47	23.066068884259895	26.133164888931148	26.293315983755843	24.507450243053107
48-49	22.917295730621433	25.947301242677817	26.396768402894434	24.73863462380632
50-51	23.146698519345566	26.020029660738192	26.27074273645628	24.562529083459964
52-53	23.440056864285328	26.253566593616007	25.942878439841078	24.36349810225759
54-55	22.996078009931388	26.18003678103137	26.260265795532323	24.563619413504924
56-57	23.068840452143743	26.295183230244675	26.230888750834435	24.40508756677715
58-59	23.12609731812676	26.206402197870705	26.14467070145209	24.522829782550446
60-61	23.179838972653965	25.99203314292084	26.133706070577702	24.694421813847498
62-63	23.268756291766564	25.958336016444118	26.19091145485854	24.58199623693078
64-65	23.316871770196407	26.191422624613107	25.87706993876248	24.614635666427997
66-67	23.418124333451637	26.092597026623704	25.934988483967246	24.554290155957414
68-69	23.387346011143183	26.166189634267912	26.126031222927278	24.320433131661627
70-71	23.72712449921282	26.120413696138872	25.847489143478153	24.304972661170154
72-73	23.452767409782314	26.04103029075197	25.937117623506012	24.5690846759597
74-75	23.427636894242458	26.20101178242521	25.95158550613138	24.41976581720095
76-77	23.515416611295826	26.17169175716435	25.937354899952293	24.375536731587534
78-79	23.30811491548023	26.209197981175734	25.8805575497854	24.602129553558637
80-81	23.5274410302483	26.24910495707497	25.858099363119823	24.365354649556902
82-83	23.613453460792012	26.23804088530835	25.667700075571837	24.4808055783278
84-85	23.485126722972975	26.25448404874052	25.761066593762976	24.499322634523523
86-87	23.47931498842995	26.238173246043107	25.77950492625738	24.50300683926956
88-89	23.701891027231532	25.980484077621256	25.742431317309816	24.575193577837393
90-91	23.57185883530641	25.94383861926195	26.016406852035516	24.46789569339612
92-93	23.813717253336648	25.956368092974518	25.942705219620105	24.28720943406873
94-95	23.72077511064112	26.039833943740465	25.868266599756893	24.37112434586152
96-97	23.665324668078817	25.84454490475857	25.93945964314849	24.550670784014123
98-99	23.850895439597146	25.06525794026764	26.365121045849836	24.718725574285376
100-101	24.754780179978408	11.661613855062456	32.265278574054854	31.318327390904276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27132.0
1	13629.5
2	199.0
3	416.5
4	702.0
5	1026.5
6	1296.5
7	1436.5
8	1631.5
9	1861.0
10	2104.5
11	2361.0
12	2612.0
13	2836.0
14	3118.0
15	3491.5
16	4064.0
17	4835.5
18	5691.5
19	6852.0
20	8356.5
21	10468.0
22	13713.5
23	18479.5
24	24639.5
25	32138.5
26	42320.0
27	58943.5
28	80077.5
29	104681.5
30	142360.5
31	192866.5
32	273238.0
33	386049.0
34	515536.5
35	682454.0
36	920164.5
37	1223951.5
38	1606938.5
39	2104837.0
40	2592905.5
41	3049382.0
42	3573829.0
43	4038941.5
44	4425709.5
45	4725696.5
46	4859391.5
47	4865439.0
48	4754909.0
49	4597068.5
50	4385596.5
51	4122535.0
52	3722005.5
53	3173846.5
54	2701259.5
55	2381555.0
56	2104963.5
57	1795807.5
58	1499982.5
59	1212356.0
60	995101.0
61	874576.0
62	812454.5
63	748264.0
64	635592.0
65	529208.5
66	464194.0
67	410776.5
68	333483.0
69	252434.5
70	184943.0
71	134401.5
72	101919.0
73	80180.5
74	67444.0
75	50331.5
76	32101.5
77	25652.0
78	19678.0
79	15161.5
80	13068.5
81	9470.0
82	5832.5
83	3705.5
84	3264.5
85	2971.5
86	2696.0
87	2388.0
88	2091.0
89	1841.0
90	1650.5
91	1455.0
92	1306.0
93	1212.5
94	1098.0
95	1007.0
96	999.0
97	1064.5
98	1173.5
99	1617.0
100	9352.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07872909732138758
2	0.04767658007544464
3	0.032955616653457
4	0.032844582622780207
5	0.032800407793371164
6	0.03363853563783469
7	0.0322201654395118
8	0.032881593966339134
9	0.03221061412504497
10-11	0.03224583459714138
12-13	0.032227925882516086
14-15	0.032225538053899376
16-17	0.033073217212829735
18-19	0.032278070283466895
20-21	0.03227210071192513
22-23	0.034664108028709575
24-25	0.03730146473586049
26-27	0.04066054264241055
28-29	0.04373905068649774
30-31	0.04671846384299167
32-33	0.049943823347006235
34-35	0.05238239332181644
36-37	2.0725730630832647E-4
38-39	5.769898559867365E-4
40-41	7.048314962627115E-4
42-43	0.001162407238588231
44-45	9.342558261379057E-4
46-47	6.385859649402207E-4
48-49	6.254632579942627E-4
50-51	0.0010878735570956815
52-53	3.948959489500502E-4
54-55	2.6884790017871036E-5
56-57	9.499607403332026E-5
58-59	1.7924356955180163E-5
60-61	4.959237132143432E-5
62-63	5.975192994401704E-6
64-65	1.912131498573846E-5
66-67	4.7805069951157025E-6
68-69	1.4342074283805663E-5
70-71	2.4502026344471157E-5
72-73	5.976354386531763E-7
74-75	7.76959948407947E-6
76-77	3.884972607386873E-5
78-79	5.79784247816667E-5
80-81	2.8572192489043655E-4
82-83	0.0015948619334958357
84-85	1.7874379720166363E-4
86-87	0.0013289965122181412
88-89	5.637954489606293E-4
90-91	0.0022978256351072262
92-93	0.001042974814388907
94-95	0.0011543596920802343
96-97	0.0018767016253942712
98-99	0.0017416475269208206
100-101	3.5536679829860466E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	45250.0
36-37	1994.0
38-39	2366.0
40-41	2256.0
42-43	2640.0
44-45	2479.0
46-47	2518.0
48-49	2523.0
50-51	2506.0
52-53	2496.0
54-55	2746.0
56-57	2648.0
58-59	2746.0
60-61	2856.0
62-63	3036.0
64-65	3153.0
66-67	3180.0
68-69	3311.0
70-71	3471.0
72-73	3623.0
74-75	3689.0
76-77	3831.0
78-79	3988.0
80-81	4144.0
82-83	4257.0
84-85	4662.0
86-87	4771.0
88-89	5878.0
90-91	15111.0
92-93	10052.0
94-95	31525.0
96-97	599160.0
98-99	7541852.0
100-101	7.5427387E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.933672825811524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.65474109013086	14.995580742371217
2	14.724438185654257	7.048197721586696
3	6.9709260685939025	5.005195914559411
4	3.7952359428224463	3.6333574140909097
5	2.4034460076136197	2.8761645200363644
6	1.6714821851951551	2.400282465278001
7	1.1759012300970693	1.9700544721628794
8	0.8700311226430671	1.6658432190090136
9	0.735520326932427	1.584333257538117
>10	4.2612227845172965	19.28019958620401
>50	0.39476510092203976	6.543936239091337
>100	0.28541593634015344	13.489151706044122
>500	0.031908373504902214	5.292925071608086
>1k	0.023165410382338093	10.428817989994611
>5k	0.0014322621591780322	2.2589336588543687
>10k+	3.6797249148842726E-4	1.5270260215706657
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7756572334104265E-6
2	2.3878286167052132E-6	7.16348585011564E-6	5.969571541763033E-6	1.1939143083526066E-6	5.969571541763033E-6
3	8.357400158468245E-6	7.16348585011564E-6	7.16348585011564E-6	1.1939143083526066E-6	1.0745228775173459E-5
4	1.9102628933641706E-5	7.16348585011564E-6	1.5520886008583887E-5	1.1939143083526066E-6	1.5520886008583887E-5
5	3.1041772017167774E-5	7.16348585011564E-6	2.6266114783757344E-5	2.3878286167052132E-6	2.2684371858699525E-5
6	3.462351494222559E-5	7.16348585011564E-6	3.58174292505782E-5	2.3878286167052132E-6	2.3878286167052132E-5
7	3.7011343558930804E-5	7.16348585011564E-6	3.7011343558930804E-5	2.3878286167052132E-6	2.746002909210995E-5
8	4.178700079234123E-5	7.16348585011564E-6	3.7011343558930804E-5	2.3878286167052132E-6	2.746002909210995E-5
9	4.895048664245687E-5	7.16348585011564E-6	4.178700079234123E-5	2.3878286167052132E-6	3.1041772017167774E-5
10-11	5.8501801109277725E-5	7.16348585011564E-6	4.5965700871575354E-5	2.9847857708815165E-6	3.4026557788049286E-5
12-13	7.16348585011564E-5	8.357400158468245E-6	5.3726143875867295E-5	5.37261438758673E-6	3.760830071310711E-5
14-15	9.01405302806218E-5	8.95435731264455E-6	6.447137265104075E-5	5.969571541763033E-6	4.5965700871575354E-5
16-17	1.092431592142635E-4	9.551314466820853E-6	7.820138719709572E-5	5.969571541763033E-6	5.014440095080948E-5
18-19	1.1879447368108435E-4	9.551314466820853E-6	9.730401613073744E-5	5.969571541763033E-6	5.1935272413338384E-5
20-21	1.289427453020815E-4	1.0745228775173459E-5	1.1163098783096872E-4	8.357400158468245E-6	5.43231010300436E-5
22-23	1.3968797407725497E-4	1.1939143083526066E-5	1.229731737603185E-4	1.3133057391878672E-5	5.730788680092512E-5
24-25	1.6476017455265972E-4	1.1939143083526066E-5	1.253610023770237E-4	1.3133057391878672E-5	5.790484395510142E-5
26-27	2.0714413249917725E-4	1.2536100237702368E-5	1.313305739187867E-4	1.4923928854407583E-5	5.8501801109277725E-5
28-29	2.6743680507098386E-4	1.432697170023128E-5	1.3610623115219714E-4	1.5520886008583887E-5	6.865007273027488E-5
30-31	3.265355633344379E-4	1.6117843162760187E-5	1.4207580269396018E-4	1.5520886008583887E-5	7.34257299636853E-5
32-33	3.814556215186578E-4	1.7311757471112795E-5	1.4923928854407582E-4	1.5520886008583887E-5	7.760443004291943E-5
34-35	4.4473307986134594E-4	1.9102628933641706E-5	1.7311757471112795E-4	1.6117843162760187E-5	8.297704443050615E-5
36-37	5.020409666622711E-4	2.0296543241994313E-5	1.7908714625289098E-4	1.671480031693649E-5	8.954357312644549E-5
38-39	5.766606109343089E-4	2.208741470452322E-5	1.8445976064047773E-4	1.7311757471112795E-5	1.0267663051832416E-4
40-41	6.590406982106389E-4	2.3878286167052132E-5	1.9520498941565117E-4	1.9102628933641706E-5	1.1282490213932132E-4
42-43	7.390329568702635E-4	2.6266114783757344E-5	2.1251674688676397E-4	1.9102628933641706E-5	1.1819751652690806E-4
44-45	8.279795728425327E-4	2.865394340046256E-5	2.1490457550346918E-4	2.0296543241994313E-5	1.2177925945196588E-4
46-47	9.282683747441516E-4	3.044481486299147E-5	2.2684371858699526E-4	2.0296543241994313E-5	1.2655491668537628E-4
48-49	0.0010404963197292966	3.1041772017167774E-5	2.3161937582040569E-4	2.0296543241994313E-5	1.31927531072963E-4
50-51	0.0011342185929349763	3.1041772017167774E-5	2.4176764744140284E-4	2.2684371858699525E-5	1.4386667415648908E-4
52-53	0.0012398800092241818	3.1041772017167774E-5	2.572885334499867E-4	2.566915762958104E-5	1.45657545619018E-4
54-55	0.0013419596825883298	3.1041772017167774E-5	2.728094194585706E-4	2.865394340046256E-5	1.5580581724001515E-4
56-57	0.0014583663276527088	3.1041772017167774E-5	2.925090055463886E-4	2.865394340046256E-5	1.7490844617365687E-4
58-59	0.0015896969015714957	3.2235686325520374E-5	3.0444814862991466E-4	2.9250900554638863E-5	1.9281716079894596E-4
60-61	0.0017096852895609328	3.342960063387298E-5	3.3310209203037724E-4	2.9847857708815166E-5	1.958019465698275E-4
62-63	0.001828479763242017	3.342960063387298E-5	3.6891952128095546E-4	2.9847857708815166E-5	1.993836894948853E-4
64-65	0.0019454833654605724	3.342960063387298E-5	3.9040997883130234E-4	2.9847857708815166E-5	2.202771898910559E-4
66-67	0.0020481599959788965	3.462351494222559E-5	3.9279780744800756E-4	2.9847857708815166E-5	2.2982850435787676E-4
68-69	0.0021681483839683336	3.462351494222559E-5	3.999612932981232E-4	2.9847857708815166E-5	2.4117069028722655E-4
70-71	0.002297091129270415	3.58174292505782E-5	4.0294607906900476E-4	2.9847857708815166E-5	2.626611478375734E-4
72-73	0.0024373760605018464	3.760830071310711E-5	4.190639222317649E-4	2.9847857708815166E-5	2.901211769296834E-4
74-75	0.0026325810499174974	3.820525786728341E-5	4.2145175084847014E-4	2.9847857708815166E-5	2.966877056256227E-4
76-77	0.002793162524390923	3.8802215021459716E-5	4.2921219385276207E-4	3.044481486299147E-5	2.9907553424232794E-4
78-79	0.0029280748412347677	3.939917217563602E-5	4.333908939319962E-4	3.1041772017167774E-5	3.104177201716777E-4
80-81	0.00305045105784091	3.999612932981232E-5	4.333908939319962E-4	3.1041772017167774E-5	3.139994630967355E-4
82-83	0.003145964202509118	4.059308648398863E-5	4.345848082403488E-4	3.1041772017167774E-5	3.241477347177327E-4
84-85	0.003271325204886142	4.059308648398863E-5	4.357787225487014E-4	3.163872917134408E-5	3.313112205678483E-4
86-87	0.0033393783204622406	4.119004363816493E-5	4.3876350831958293E-4	3.223568632552038E-5	3.3548992064708245E-4
88-89	0.003351317463545767	4.2980915100693835E-5	4.4294220839881707E-4	3.223568632552038E-5	3.4384732080555073E-4
90	0.003351317463545767	4.417482940904644E-5	4.4413612270716965E-4	3.223568632552038E-5	3.510108066556663E-4
>>END_MODULE
