##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290310_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84987041
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.05195288538167	37.0	37.0	37.0	37.0	37.0
2	36.17930493661969	37.0	37.0	37.0	37.0	37.0
3	36.29719067404641	37.0	37.0	37.0	37.0	37.0
4	36.329664601453764	37.0	37.0	37.0	37.0	37.0
5	36.34609869521166	37.0	37.0	37.0	37.0	37.0
6	36.31334965527274	37.0	37.0	37.0	37.0	37.0
7	36.27765724894458	37.0	37.0	37.0	37.0	37.0
8	36.337917377309324	37.0	37.0	37.0	37.0	37.0
9	36.31807395200405	37.0	37.0	37.0	37.0	37.0
10-11	36.33967980483048	37.0	37.0	37.0	37.0	37.0
12-13	36.32098106580743	37.0	37.0	37.0	37.0	37.0
14-15	36.31593846172383	37.0	37.0	37.0	37.0	37.0
16-17	36.31464826502196	37.0	37.0	37.0	37.0	37.0
18-19	36.311015064049585	37.0	37.0	37.0	37.0	37.0
20-21	36.30412646088008	37.0	37.0	37.0	37.0	37.0
22-23	36.291659701389065	37.0	37.0	37.0	37.0	37.0
24-25	36.260995691096014	37.0	37.0	37.0	37.0	37.0
26-27	36.215981904817696	37.0	37.0	37.0	37.0	37.0
28-29	36.20180406681061	37.0	37.0	37.0	37.0	37.0
30-31	36.1923663573603	37.0	37.0	37.0	37.0	37.0
32-33	36.18832792990169	37.0	37.0	37.0	37.0	37.0
34-35	36.17442941683309	37.0	37.0	37.0	37.0	37.0
36-37	36.18213422199444	37.0	37.0	37.0	37.0	37.0
38-39	36.17652657499837	37.0	37.0	37.0	37.0	37.0
40-41	36.17636784883341	37.0	37.0	37.0	37.0	37.0
42-43	36.15812135857819	37.0	37.0	37.0	37.0	37.0
44-45	36.156194988899344	37.0	37.0	37.0	37.0	37.0
46-47	36.15361906920047	37.0	37.0	37.0	37.0	37.0
48-49	36.12827221313594	37.0	37.0	37.0	37.0	37.0
50-51	36.12149985225625	37.0	37.0	37.0	37.0	37.0
52-53	36.12239925509332	37.0	37.0	37.0	37.0	37.0
54-55	36.11161574559136	37.0	37.0	37.0	37.0	37.0
56-57	36.09636360927898	37.0	37.0	37.0	37.0	37.0
58-59	36.08379422008913	37.0	37.0	37.0	37.0	37.0
60-61	36.08087110877143	37.0	37.0	37.0	37.0	37.0
62-63	36.07009006251333	37.0	37.0	37.0	37.0	37.0
64-65	36.054631981202476	37.0	37.0	37.0	37.0	37.0
66-67	36.053405044054955	37.0	37.0	37.0	37.0	37.0
68-69	36.03823027380116	37.0	37.0	37.0	37.0	37.0
70-71	36.02050637063026	37.0	37.0	37.0	37.0	37.0
72-73	35.99724672671216	37.0	37.0	37.0	37.0	37.0
74-75	35.993463804162275	37.0	37.0	37.0	37.0	37.0
76-77	35.980393079265376	37.0	37.0	37.0	37.0	37.0
78-79	35.96683234342247	37.0	37.0	37.0	37.0	37.0
80-81	35.940008627661555	37.0	37.0	37.0	37.0	37.0
82-83	35.952450230553715	37.0	37.0	37.0	37.0	37.0
84-85	35.934901986506475	37.0	37.0	37.0	37.0	37.0
86-87	35.947910170366114	37.0	37.0	37.0	37.0	37.0
88-89	35.92313769821692	37.0	37.0	37.0	37.0	37.0
90-91	35.90484201486337	37.0	37.0	37.0	37.0	37.0
92-93	35.89548853964024	37.0	37.0	37.0	37.0	37.0
94-95	35.86771283316381	37.0	37.0	37.0	37.0	37.0
96-97	35.8690184998526	37.0	37.0	37.0	37.0	37.0
98-99	35.847328264752505	37.0	37.0	37.0	37.0	37.0
100-101	35.8228522470603	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53679.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	6.0
15	76.0
16	387.0
17	1143.0
18	1958.0
19	2814.0
20	4198.0
21	7471.0
22	14080.0
23	28689.0
24	54784.0
25	98142.0
26	158947.0
27	257390.0
28	389231.0
29	571453.0
30	778282.0
31	1061416.0
32	1401390.0
33	2008677.0
34	3177436.0
35	7387365.0
36	5.0370563E7
37	1.7157463E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.941667299941077	16.69141580009365	11.841310792753243	46.52560610721203
2	21.749213928494235	19.653698745681535	32.09126412996414	26.505823195860096
3	23.35010057590393	19.863738082595468	23.521753446768408	33.264407894732194
4	24.57944233666774	25.012918657791456	21.02684764887257	29.380791356668233
5	25.875783900074666	29.08335593733523	23.263184357595836	21.777675804994264
6	21.38974317300662	33.60432500011009	22.958748530406698	22.047183296476593
7	18.82353367808518	22.09230219804557	38.07942984760217	21.004734276267083
8	19.74265071480392	24.20877440363187	29.273247184068847	26.77532769749536
9	20.70119395485604	22.28740338808206	31.58540103475475	25.426001622307144
10-11	22.631804700206054	30.474777570177768	23.995730464524236	22.89768726509194
12-13	22.014263704082843	25.735822369303325	27.48546571900895	24.764448207604886
14-15	22.067965064625668	26.248838470648057	26.96093387748751	24.72226258723877
16-17	22.85917085059422	26.253221437599176	26.387733963738203	24.499873748068396
18-19	22.65406610543267	26.669940298140887	25.900531524768507	24.775462071657937
20-21	22.749494112070508	26.476203919787118	26.193191373135384	24.581110595007
22-23	22.59815119098421	26.566027120594	26.352979646956324	24.482842041465464
24-25	22.59710048822266	26.358881408733776	26.42427741877239	24.619740684271168
26-27	22.75733921869639	26.565521408738135	26.281748451124194	24.395390921441283
28-29	22.58491559414913	26.41979111738111	26.272147633781213	24.72314565468855
30-31	22.617816697905834	26.188442955434628	26.367838047315328	24.825902299344207
32-33	22.863733886912325	26.369097843716656	26.2240986372114	24.54306963215962
34-35	22.80709413482165	26.45499208554034	26.064098144598663	24.673815635039347
36-37	22.558961122502446	26.326581944759038	26.307180727257272	24.807276205481244
38-39	22.91514912438572	26.435329090246995	26.315288732240578	24.33423305312671
40-41	23.052228585075692	26.357069690431267	25.90627101369696	24.684430710796086
42-43	22.805551897146	26.387695784183858	26.17310180122815	24.633650517441993
44-45	22.97698926438093	26.312929447137524	26.297009844399234	24.413071444082316
46-47	22.938203046296636	26.10832637090217	26.277582737326206	24.675887845474993
48-49	22.75793004344913	25.97670917790957	26.397584991336814	24.867775787304492
50-51	22.976021405018923	26.006053748823966	26.26088053396711	24.757044312189997
52-53	23.204646953411974	26.235447113227366	25.938670644146722	24.62123528921394
54-55	22.7730573351964	26.17485983874618	26.26498972709238	24.787093098965045
56-57	22.923366230307423	26.295998828529534	26.205753039084506	24.57488190207854
58-59	22.93746116930494	26.1973516076214	26.148700762867104	24.716486460206557
60-61	23.006996130173963	26.07196138731725	26.125398527515937	24.79564395499286
62-63	23.088631473703508	25.97641896399072	26.206088945806798	24.728860616498974
64-65	23.120384459987584	26.188918561529167	25.947655823007686	24.743041155475566
66-67	23.185082047417644	26.07332504771775	26.02208873484629	24.71950417001832
68-69	23.150875526745768	26.222853620096053	26.13330878299966	24.492962070158512
70-71	23.516208172374796	26.113559308537752	25.93898246843699	24.431250050650466
72-73	23.25143805729966	26.001538992975583	26.008579606075514	24.738443343649237
74-75	23.330697455118944	26.132455745720833	26.03211795330505	24.50472884585517
76-77	23.370538994992597	26.087894276896588	25.94179564449596	24.599771083614854
78-79	23.118351032420126	26.16166282342865	25.885230299707533	24.834755844443688
80-81	23.329381654357896	26.196670454472027	25.862067269460436	24.611880621709645
82-83	23.39117068504427	26.224759704665697	25.6542991789351	24.729770431354932
84-85	23.260343247261485	26.24037374734483	25.802287253529954	24.69699575186373
86-87	23.311093583907127	26.189623426827175	25.796890250999887	24.702392738265814
88-89	23.448642652663057	25.965244970853114	25.761535100637374	24.82457727584646
90-91	23.34732904329475	25.958766602349105	26.009447683097946	24.684456671258204
92-93	23.579511341314323	25.944222456586047	25.970822185365332	24.505444016734298
94-95	23.46075509808456	25.98083928928798	25.948193754291278	24.610211858336175
96-97	23.410358755774592	25.800184815792637	26.057797692975278	24.731658735457497
98-99	23.586159916470432	25.010466410231412	26.4651761627597	24.93819751053845
100-101	24.447199843234042	11.655065082875952	32.33828806946213	31.559447004427877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53908.0
1	27032.0
2	169.5
3	244.0
4	303.5
5	325.5
6	333.0
7	371.5
8	440.5
9	456.5
10	457.5
11	503.5
12	588.0
13	693.0
14	863.0
15	1133.0
16	1614.5
17	2217.5
18	3060.5
19	4186.5
20	5288.0
21	7139.0
22	10815.5
23	16196.0
24	22280.0
25	29747.5
26	39749.0
27	56121.5
28	76855.5
29	99864.5
30	137561.0
31	188044.5
32	267130.5
33	380974.5
34	510451.0
35	666052.0
36	897176.0
37	1211004.0
38	1601623.5
39	2117228.0
40	2622722.5
41	3084910.5
42	3611856.0
43	4150323.0
44	4585115.0
45	4924260.0
46	5104353.0
47	5115137.0
48	4974346.0
49	4747182.0
50	4479245.0
51	4162983.0
52	3729335.0
53	3142713.5
54	2643665.5
55	2311024.0
56	2031524.0
57	1731247.5
58	1447200.5
59	1177019.0
60	986088.0
61	884629.5
62	821772.0
63	752038.5
64	646522.0
65	546258.0
66	479359.5
67	420783.5
68	342722.0
69	261381.5
70	196801.0
71	147265.0
72	111170.5
73	86753.0
74	73624.0
75	56073.0
76	35621.0
77	30568.0
78	22666.5
79	14291.5
80	12558.5
81	8966.0
82	3741.0
83	588.5
84	362.5
85	273.5
86	206.5
87	164.0
88	142.5
89	131.0
90	104.5
91	72.5
92	79.0
93	81.5
94	74.0
95	98.0
96	201.5
97	555.5
98	1680.5
99	1919.5
100	694.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09931514146962711
2	0.07318409873806525
3	0.06491224938635055
4	0.06316492416767398
5	0.06318963381723103
6	0.06316139421773727
7	0.06316139421773727
8	0.06316139421773727
9	0.06316139421773727
10-11	0.06316198254272672
12-13	0.06318963381723103
14-15	0.06325552621604981
16-17	0.06317080741756852
18-19	0.06335083486434126
20-21	0.06319081046720994
22-23	0.06364852730900468
24-25	0.06591416684338969
26-27	0.06789388043290034
28-29	0.06999478897023842
30-31	0.07154561364243756
32-33	0.0730623154652484
34-35	0.07455960256340728
36-37	1.1893181823146231E-4
38-39	2.531751072911946E-5
40-41	3.591611874076109E-5
42-43	3.603441922937922E-4
44-45	7.654498545851358E-5
46-47	1.7252342070205916E-4
48-49	1.4072963132163465E-4
50-51	6.18278270020186E-5
52-53	9.009349054510036E-5
54-55	3.0031706238847305E-5
56-57	1.177736010155335E-6
58-59	0.0
60-61	0.0
62-63	4.711227212500657E-6
64-65	1.177833660488411E-6
66-67	4.71143902412623E-6
68-69	0.0
70-71	1.7668766566198723E-6
72-73	2.944877401956123E-6
74-75	0.0
76-77	1.7670300049469773E-6
78-79	0.0
80-81	1.1780993244112848E-6
82-83	5.5961718179084624E-5
84-85	8.247309137193204E-6
86-87	9.425901673211836E-6
88-89	2.6923935216982114E-4
90-91	4.6545571508208965E-5
92-93	3.5357089663664214E-6
94-95	5.846364346097909E-4
96-97	2.301183906015401E-5
98-99	1.740999448589454E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	64185.0
36-37	1092.0
38-39	1363.0
40-41	1234.0
42-43	1443.0
44-45	1367.0
46-47	1429.0
48-49	1331.0
50-51	1441.0
52-53	1520.0
54-55	1586.0
56-57	1570.0
58-59	1686.0
60-61	1738.0
62-63	1947.0
64-65	1853.0
66-67	2043.0
68-69	2071.0
70-71	2383.0
72-73	2396.0
74-75	2524.0
76-77	2669.0
78-79	2921.0
80-81	3059.0
82-83	3152.0
84-85	3630.0
86-87	3697.0
88-89	4530.0
90-91	13805.0
92-93	9026.0
94-95	31239.0
96-97	611900.0
98-99	7623774.0
100-101	7.6575437E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.02649268180238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.28882856793902	10.329259994515157
2	16.812914225823853	6.397815789548174
3	8.541672245384255	4.875541934014741
4	4.913318023544912	3.7393283767338006
5	3.1776864561959455	3.023011405193735
6	2.098133314035598	2.3952070886966452
7	1.5346186500290768	2.0438887359894973
8	1.106731197768738	1.6845770428055424
9	0.8607711599683601	1.4739710558260093
>10	5.6563402744454905	20.48666434942871
>50	0.5445469876036036	7.187524334732041
>100	0.38589753285371664	14.440013573383306
>500	0.04327116564210072	5.68259233282972
>1k	0.03273573951088306	12.077335313246358
>5k	0.0020486878972147786	2.63476531081555
>10k+	4.857713570715455E-4	1.5285033622410151
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1766499789067843E-6	0.0	0.0	0.0	4.706599915627137E-6
2	1.1766499789067843E-6	0.0	2.3532999578135685E-6	0.0	5.883249894533921E-6
3	3.5299499367203526E-6	0.0	2.3532999578135685E-6	0.0	7.059899873440705E-6
4	1.2943149767974626E-5	0.0	1.0589849810161058E-5	0.0	9.413199831254274E-6
5	2.7062949514856036E-5	0.0	1.411979974688141E-5	0.0	9.413199831254274E-6
6	3.294619940938995E-5	0.0	2.0003049641415332E-5	0.0	9.413199831254274E-6
7	3.294619940938995E-5	0.0	2.470964955704247E-5	0.0	9.413199831254274E-6
8	4.1182749261737445E-5	0.0	2.9416249472669605E-5	0.0	1.0589849810161058E-5
9	4.353604921955101E-5	0.0	3.294619940938995E-5	0.0	1.5296449725788193E-5
10-11	5.471422401916546E-5	0.0	4.412437420900441E-5	0.0	2.1768024609775507E-5
12-13	6.412742385041973E-5	0.0	5.765584896643242E-5	0.0	2.5886299535949252E-5
14-15	8.413047349183506E-5	2.3532999578135685E-6	8.94253983969156E-5	0.0	3.0004574462122995E-5
16-17	9.589697328090291E-5	2.3532999578135685E-6	1.1060509801723772E-4	1.7649749683601765E-6	3.118122444102978E-5
18-19	1.0295687315434361E-4	2.3532999578135685E-6	1.1884164786958519E-4	2.3532999578135685E-6	3.176954943048317E-5
20-21	1.1060509801723771E-4	2.3532999578135685E-6	1.317847976375598E-4	3.5299499367203526E-6	3.412284938829674E-5
22-23	1.1472337294341146E-4	2.3532999578135685E-6	1.5061119730006838E-4	3.5299499367203526E-6	3.412284938829674E-5
24-25	1.329614476164666E-4	4.706599915627137E-6	1.5296449725788193E-4	3.5299499367203526E-6	3.412284938829674E-5
26-27	1.5355282224733532E-4	5.883249894533921E-6	1.5649444719460228E-4	3.5299499367203526E-6	3.941777429337727E-5
28-29	1.9061729658289902E-4	5.883249894533921E-6	1.6473099704694978E-4	4.706599915627137E-6	4.47126991984578E-5
30-31	2.200335460555686E-4	7.059899873440704E-6	1.6767262199421674E-4	4.706599915627137E-6	4.765432414572476E-5
32-33	2.4003659569698396E-4	8.236549852347488E-6	1.7237922190984386E-4	4.706599915627137E-6	5.118427408244511E-5
34-35	2.612162953173061E-4	8.236549852347488E-6	1.8532237167781849E-4	4.706599915627137E-6	5.412589902971207E-5
36-37	2.9886909464232317E-4	1.0589849810161058E-5	2.000304964141533E-4	7.059899873440705E-6	6.118579890315277E-5
38-39	3.253437191677258E-4	1.0589849810161058E-5	2.0473709632978044E-4	7.059899873440705E-6	7.41289486711274E-5
40-41	3.4475844381968773E-4	1.0589849810161058E-5	2.1709192110830168E-4	7.648224862894096E-6	8.530712347074185E-5
42-43	3.6181986851383614E-4	1.2943149767974624E-5	2.2650512093955595E-4	9.413199831254274E-6	9.001372338636899E-5
44-45	3.924127679654125E-4	1.5296449725788193E-5	2.3532999578135684E-4	1.2943149767974626E-5	9.119037336527576E-5
46-47	4.2535896737480247E-4	1.706142469414837E-5	2.523914204755052E-4	1.2943149767974626E-5	9.354367332308934E-5
48-49	4.736016165099806E-4	1.8826399662508548E-5	2.5886299535949253E-4	1.411979974688141E-5	1.0119189818598343E-4
50-51	5.083127908877308E-4	1.8826399662508548E-5	2.6768787020129336E-4	1.411979974688141E-5	1.0707514808051736E-4
52-53	5.459655902127479E-4	1.8826399662508548E-5	2.841609699059884E-4	1.411979974688141E-5	1.0825179805942415E-4
54-55	5.795001146115912E-4	1.8826399662508548E-5	3.012223946001367E-4	1.411979974688141E-5	1.117817479961445E-4
56-57	6.218595138522354E-4	1.8826399662508548E-5	3.2122544424155203E-4	1.411979974688141E-5	1.264898727324793E-4
58-59	6.701021629874136E-4	1.8826399662508548E-5	3.2946199409389956E-4	1.411979974688141E-5	1.50022872310615E-4
60-61	6.995184124600831E-4	1.941472465196194E-5	3.482883937564081E-4	1.411979974688141E-5	1.5767109717350906E-4
62-63	7.389361867534605E-4	2.0003049641415332E-5	3.7299804331345057E-4	1.5296449725788193E-5	1.6120104711022943E-4
64-65	7.865905108991853E-4	2.0591374630868726E-5	3.9064779299705234E-4	1.5296449725788193E-5	1.6649597201530997E-4
66-67	8.254199602031091E-4	2.1179699620322116E-5	3.918244429759591E-4	1.5296449725788193E-5	1.7296754689929728E-4
68-69	8.813108342011814E-4	2.1179699620322116E-5	4.012376428072134E-4	1.5884774715241583E-5	1.8002744677273798E-4
70-71	9.183753085367451E-4	2.23563495992289E-5	4.0535591773338715E-4	1.6473099704694977E-5	1.912056215723524E-4
72-73	9.636763327246561E-4	2.2944674588682294E-5	4.1065084263846766E-4	1.6473099704694977E-5	2.0297212136142026E-4
74-75	0.0010095656819020208	2.3532999578135684E-5	4.1065084263846766E-4	1.6473099704694977E-5	2.082670462665008E-4
76-77	0.0010489834561953982	2.3532999578135684E-5	4.135924675857346E-4	1.6473099704694977E-5	2.0885537125595418E-4
78-79	0.0010854596055415084	2.3532999578135684E-5	4.17122417522455E-4	1.6473099704694977E-5	2.1179699620322114E-4
80-81	0.001128407329771606	2.3532999578135684E-5	4.1947571748026855E-4	1.6473099704694977E-5	2.1768024609775507E-4
82-83	0.001164295154128263	2.3532999578135684E-5	4.241823173958957E-4	1.6473099704694977E-5	2.2415182098174238E-4
84-85	0.001199006328506013	2.3532999578135684E-5	4.3065389227988304E-4	1.6473099704694977E-5	2.2709344592900934E-4
86-87	0.0012137144532423478	2.3532999578135684E-5	4.406554171005907E-4	1.6473099704694977E-5	2.3415334580245006E-4
88-89	0.0012166560781896147	3.0004574462122998E-5	4.418320670794974E-4	1.6473099704694977E-5	2.453315206020645E-4
90	0.0012166560781896147	3.176954943048317E-5	4.435970420478576E-4	1.6473099704694977E-5	2.470964955704247E-4
>>END_MODULE
