##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290301_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88528711
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04659967318399	37.0	37.0	37.0	37.0	37.0
2	36.11709586509172	37.0	37.0	37.0	37.0	37.0
3	36.18291066047488	37.0	37.0	37.0	37.0	37.0
4	36.164362835916585	37.0	37.0	37.0	37.0	37.0
5	36.134597407613896	37.0	37.0	37.0	37.0	37.0
6	36.059359748274204	37.0	37.0	37.0	37.0	37.0
7	36.01853990622319	37.0	37.0	37.0	37.0	37.0
8	36.090532302000874	37.0	37.0	37.0	37.0	37.0
9	36.03513928944476	37.0	37.0	37.0	37.0	37.0
10-11	36.065948322685955	37.0	37.0	37.0	37.0	37.0
12-13	36.04811624332811	37.0	37.0	37.0	37.0	37.0
14-15	36.03713309459572	37.0	37.0	37.0	37.0	37.0
16-17	36.018851799389694	37.0	37.0	37.0	37.0	37.0
18-19	36.024615759965144	37.0	37.0	37.0	37.0	37.0
20-21	36.01655743073002	37.0	37.0	37.0	37.0	37.0
22-23	35.98446175840061	37.0	37.0	37.0	37.0	37.0
24-25	35.972888174097555	37.0	37.0	37.0	37.0	37.0
26-27	35.9450591345445	37.0	37.0	37.0	37.0	37.0
28-29	35.9274704056179	37.0	37.0	37.0	37.0	37.0
30-31	35.90685523479496	37.0	37.0	37.0	37.0	37.0
32-33	35.883121352574534	37.0	37.0	37.0	37.0	37.0
34-35	35.86589917139989	37.0	37.0	37.0	37.0	37.0
36-37	35.84694933483465	37.0	37.0	37.0	37.0	37.0
38-39	35.83159703077115	37.0	37.0	37.0	37.0	37.0
40-41	35.83261468739034	37.0	37.0	37.0	37.0	37.0
42-43	35.794519673658456	37.0	37.0	37.0	37.0	37.0
44-45	35.78203770942809	37.0	37.0	37.0	37.0	37.0
46-47	35.78423032768025	37.0	37.0	37.0	37.0	37.0
48-49	35.75801591267074	37.0	37.0	37.0	37.0	37.0
50-51	35.73789163815596	37.0	37.0	37.0	37.0	37.0
52-53	35.72454116281282	37.0	37.0	37.0	37.0	37.0
54-55	35.71903605712811	37.0	37.0	37.0	37.0	37.0
56-57	35.68517115663299	37.0	37.0	37.0	37.0	37.0
58-59	35.706494946609666	37.0	37.0	37.0	37.0	37.0
60-61	35.66935503000909	37.0	37.0	37.0	37.0	37.0
62-63	35.65851067272702	37.0	37.0	37.0	37.0	37.0
64-65	35.64614886713272	37.0	37.0	37.0	37.0	37.0
66-67	35.625710688846425	37.0	37.0	37.0	37.0	37.0
68-69	35.59076258905844	37.0	37.0	37.0	37.0	37.0
70-71	35.59268943438564	37.0	37.0	37.0	37.0	37.0
72-73	35.569588500267145	37.0	37.0	37.0	37.0	37.0
74-75	35.584135037742286	37.0	37.0	37.0	37.0	37.0
76-77	35.558005910884546	37.0	37.0	37.0	37.0	37.0
78-79	35.51067588159493	37.0	37.0	37.0	37.0	37.0
80-81	35.50413630140589	37.0	37.0	37.0	37.0	37.0
82-83	35.469405882173916	37.0	37.0	37.0	37.0	37.0
84-85	35.42493811230369	37.0	37.0	37.0	37.0	37.0
86-87	35.38560298869817	37.0	37.0	37.0	37.0	37.0
88-89	35.409948783987986	37.0	37.0	37.0	37.0	37.0
90-91	35.347904320192875	37.0	37.0	37.0	37.0	37.0
92-93	35.38776291553559	37.0	37.0	37.0	37.0	37.0
94-95	35.33612232186309	37.0	37.0	37.0	37.0	37.0
96-97	35.30940157882454	37.0	37.0	37.0	37.0	37.0
98-99	35.303447193030806	37.0	37.0	37.0	37.0	37.0
100-101	35.28215993102739	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8027.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	42.0
12	1208.0
13	9188.0
14	20939.0
15	25691.0
16	25996.0
17	28586.0
18	31285.0
19	37279.0
20	49169.0
21	73937.0
22	105817.0
23	146140.0
24	182223.0
25	222757.0
26	270096.0
27	347962.0
28	447921.0
29	598346.0
30	801553.0
31	1146073.0
32	1676925.0
33	2794681.0
34	5224506.0
35	1.3622398E7
36	5.1290255E7
37	9339711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.359622478098075	16.042003363392972	12.929300087291795	45.66907407121715
2	22.50261188770119	19.591035579295383	32.04195746707476	25.86439506592867
3	24.33019504013698	20.319331278693735	23.142571702875912	32.20790197829337
4	25.232042067345656	25.359699998721197	20.543251189028634	28.865006744904516
5	26.29897588207489	29.33127099373062	22.881417193732002	21.488335930462497
6	21.823430140699383	33.77667662011949	22.624022182897242	21.775871056283886
7	19.134737250977942	22.10205769789005	38.08283787090998	20.68036718022204
8	20.03850356802547	24.17948812263085	29.506180674790862	26.27582763455282
9	21.019391829809898	22.482500756150976	31.47572902793496	25.022378386104165
10-11	22.858221991983406	30.898375607218505	23.750928413345193	22.492473987452893
12-13	22.188898112849632	26.067475636366115	27.35317546540716	24.39045078537709
14-15	22.298512374601042	26.668087201145514	26.87853126555569	24.154869158697757
16-17	23.01960277880716	26.587953653304346	26.275199206705153	24.11724436118334
18-19	22.91514273976101	26.91933453134042	25.938476087874324	24.22704664102424
20-21	23.01909318700873	26.79218301568178	26.219491110055664	23.96923268725383
22-23	22.88298968323011	26.890305367380446	26.289196887219763	23.93750806216968
24-25	22.752253059071606	26.82776411123498	26.35849474059806	24.061488089095352
26-27	22.79435358313125	27.005050200540857	26.26955126383462	23.931044952493266
28-29	22.69874394207408	26.921835326023135	26.198334134661565	24.181086597241215
30-31	22.69759419967566	26.70664964844619	26.31218688614444	24.28356926573371
32-33	22.946593029513686	26.81449749695109	26.182654287047157	24.05625518648807
34-35	22.901258083815588	26.844845077711714	26.04410157080149	24.209795267671204
36-37	22.713796685328298	26.761945167374652	26.239622087704156	24.2846360595929
38-39	22.899167075376212	26.872085414737267	26.234055066966743	23.994692442919778
40-41	23.05874213118918	26.73043561496265	26.067720003425247	24.14310225042292
42-43	22.897108535517408	26.680756630755187	26.30758793613939	24.114546897588006
44-45	23.00475355294775	26.707499317220208	26.302434057493922	23.98531307233812
46-47	23.022821863766428	26.668444850590912	26.196639496783142	24.11209378885952
48-49	22.88073764452183	26.55296087251593	26.279690041269692	24.286611441692546
50-51	23.0273189729656	26.615850417105502	26.184978479652273	24.171852130276623
52-53	23.27619136447249	26.72574035833023	25.941284056544212	24.056784220653075
54-55	22.95800695215741	26.658374589608997	26.180127519782744	24.20349093845085
56-57	22.995834040438915	26.72312380322351	26.201824144529844	24.07921801180773
58-59	23.106785968808406	26.64470845995006	26.153039779571653	24.09546579166988
60-61	23.075881198296557	26.51427826198789	26.173953324689464	24.235887215026093
62-63	23.176151924026346	26.4395896859614	26.227957363241668	24.15630102677059
64-65	23.231828108113344	26.616157072300194	26.009172600835377	24.14284221875108
66-67	23.247441824939198	26.56907772557097	26.062990431534033	24.120490017955802
68-69	23.253570181478807	26.618887773964417	26.102371428448066	24.02517061610871
70-71	23.453854712362414	26.567976272972	25.93280695347062	24.045362061194965
72-73	23.259442105714296	26.55931829437882	26.029103765777073	24.15213583412981
74-75	23.288459424135883	26.567500955716067	26.069404395758085	24.07463522438997
76-77	23.40206899865974	26.54505628214959	25.937493291319342	24.115381427871323
78-79	23.196982114986454	26.532120370925643	26.034743046455628	24.23615446763227
80-81	23.33133718801764	26.62893861006046	25.971591128499565	24.068133073422334
82-83	23.4116400450147	26.6027964339774	25.852067301473735	24.133496219534162
84-85	23.35746699738191	26.537919303366497	25.963688434399963	24.140925264851635
86-87	23.38867915084457	26.532970389139194	25.95715035894119	24.12120010107504
88-89	23.534166340519995	26.397706322055072	25.881955859452322	24.186171477972614
90-91	23.396335066393767	26.398540745395078	26.048617689658705	24.156506498552453
92-93	23.53627061402516	26.473158157528438	25.997655727266526	23.992915501179883
94-95	23.514115724937135	26.443229245805718	25.978900982938985	24.063754046318163
96-97	23.38704266356789	26.3169347363586	26.045664451549754	24.25035814852376
98-99	23.607772629962717	25.517009487429494	26.507552968771392	24.3676649138364
100-101	24.467848382375443	12.0466261329187	32.716780981523904	30.768744503181956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8441.0
1	4402.0
2	526.5
3	1022.5
4	1686.5
5	2269.0
6	2810.0
7	3325.5
8	3737.0
9	4074.0
10	4429.0
11	4833.5
12	5305.0
13	5834.0
14	6412.0
15	7040.5
16	7817.5
17	8802.0
18	9899.5
19	11504.5
20	13412.0
21	16122.5
22	20050.0
23	25809.5
24	33824.5
25	43727.5
26	57445.0
27	78220.0
28	106281.5
29	143165.5
30	195147.0
31	264993.5
32	362512.0
33	488050.5
34	650049.0
35	870098.5
36	1149489.0
37	1486413.5
38	1907647.0
39	2410033.5
40	2895737.5
41	3377308.5
42	3907837.0
43	4379263.5
44	4768132.5
45	5041778.0
46	5173518.0
47	5199209.5
48	5049234.5
49	4750029.5
50	4405545.5
51	4039678.5
52	3633669.0
53	3188344.0
54	2741000.0
55	2374796.0
56	2062271.0
57	1739926.0
58	1443586.5
59	1196529.0
60	1005590.0
61	872089.5
62	780916.5
63	692046.0
64	604155.5
65	533970.5
66	476181.5
67	415472.5
68	334948.0
69	258243.0
70	205086.0
71	161092.5
72	123834.5
73	96643.0
74	81938.0
75	64617.0
76	42141.0
77	32897.5
78	26113.5
79	20919.5
80	18069.0
81	12623.5
82	6646.0
83	2959.5
84	2477.0
85	2171.5
86	1932.5
87	1757.5
88	1623.5
89	1494.5
90	1361.0
91	1256.0
92	1231.0
93	1176.0
94	1109.0
95	1095.5
96	1104.5
97	1155.5
98	1299.0
99	1834.0
100	9513.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05603379902368623
2	0.02467335145092082
3	0.009856689317435108
4	0.009663531642293988
5	0.009639810524294202
6	0.010555897509905007
7	0.009080669885727807
8	0.009717751340579216
9	0.009069374115251718
10-11	0.00910890931191803
12-13	0.009091965656203896
14-15	0.009080669885727807
16-17	0.009925028728815446
18-19	0.009136583949584446
20-21	0.009124723390584552
22-23	0.010821912904616898
24-25	0.010862012889807014
26-27	0.012244615196080286
28-29	0.013704593530114767
30-31	0.015119388782244893
32-33	0.01701030075994216
34-35	0.018395727008834458
36-37	1.8698180900866832E-4
38-39	5.072889255658405E-4
40-41	6.485284262945919E-4
42-43	0.0010824062974398386
44-45	8.683146929274858E-4
46-47	5.886819267032924E-4
48-49	5.920848961154282E-4
50-51	9.887145522103884E-4
52-53	3.5368651579876424E-4
54-55	2.655543187629969E-5
56-57	8.814386430193329E-5
58-59	1.5821143867119477E-5
60-61	4.237927535903086E-5
62-63	7.345969900894164E-6
64-65	1.5822601423171795E-5
66-67	9.04181337997824E-6
68-69	1.6388927376181273E-5
70-71	2.9388290974689686E-5
72-73	5.651859728618494E-7
74-75	4.5216915150571765E-6
76-77	2.882718187555716E-5
78-79	5.935320472598027E-5
80-81	2.775635438205282E-4
82-83	0.0015592092674288178
84-85	1.7470309971469457E-4
86-87	0.0012727797897361002
88-89	5.128898841026065E-4
90-91	0.002247479833675634
92-93	9.582544970080286E-4
94-95	0.0010959117958135162
96-97	0.0017956456567118282
98-99	0.001663692302656621
100-101	3.257201838300879E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	17069.0
36-37	1538.0
38-39	1650.0
40-41	1514.0
42-43	1690.0
44-45	1960.0
46-47	1982.0
48-49	1957.0
50-51	2281.0
52-53	2368.0
54-55	2408.0
56-57	2505.0
58-59	2410.0
60-61	2782.0
62-63	2833.0
64-65	3087.0
66-67	3397.0
68-69	3659.0
70-71	4207.0
72-73	3994.0
74-75	4165.0
76-77	4545.0
78-79	5096.0
80-81	5894.0
82-83	6154.0
84-85	7235.0
86-87	7748.0
88-89	8976.0
90-91	19505.0
92-93	15141.0
94-95	36843.0
96-97	639570.0
98-99	8015788.0
100-101	7.968676E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.221980826055518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.6279778957685	7.888421250350082
2	8.236376790163703	2.6722069273240856
3	7.0650477932053635	3.4382720950962975
4	6.0928991968267345	3.953555757840494
5	5.048311013095487	4.094680222919995
6	4.069590125142384	3.9610087787978783
7	3.1809531109759046	3.612095226238294
8	2.6166779262054165	3.3958159317493584
9	2.076955863735633	3.032310437827472
>10	11.910507083265447	33.632197181025255
>50	0.6379149275030962	7.118899072578497
>100	0.37906600876485763	11.880823923176225
>500	0.035844938403854346	4.013534425205506
>1k	0.021128537122298424	6.260414747157704
>5k	5.735411398264559E-4	0.6275982316719496
>10k+	1.7524868161363928E-4	0.4181657910407946
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.259154095217765E-6	0.0
2	5.647885238044412E-6	7.907039333262178E-6	3.3887311428266473E-6	2.259154095217765E-6	5.647885238044412E-6
3	5.647885238044412E-6	7.907039333262178E-6	3.3887311428266473E-6	2.259154095217765E-6	2.9369003237830945E-5
4	1.2425347523697708E-5	7.907039333262178E-6	1.9202809809351002E-5	2.259154095217765E-6	3.388731142826647E-5
5	2.0332386856959886E-5	7.907039333262178E-6	2.146196390456877E-5	2.259154095217765E-6	3.388731142826647E-5
6	4.51830819043553E-5	7.907039333262178E-6	4.066477371391977E-5	2.259154095217765E-6	3.388731142826647E-5
7	4.631265895196418E-5	9.03661638087106E-6	4.857181304718195E-5	2.259154095217765E-6	3.388731142826647E-5
8	4.7442235999573065E-5	9.03661638087106E-6	4.857181304718195E-5	2.259154095217765E-6	3.388731142826647E-5
9	4.857181304718195E-5	9.03661638087106E-6	5.19605441900086E-5	2.259154095217765E-6	3.388731142826647E-5
10-11	5.7043640904248566E-5	9.03661638087106E-6	5.873800647566189E-5	2.259154095217765E-6	4.7442235999573065E-5
12-13	6.099716057087966E-5	9.03661638087106E-6	7.51168736659907E-5	9.03661638087106E-6	5.139575566620415E-5
14-15	7.172814252316405E-5	9.03661638087106E-6	1.1465207033230157E-4	9.03661638087106E-6	6.551546876131518E-5
16-17	8.923658676110173E-5	9.03661638087106E-6	1.3441966866545703E-4	9.03661638087106E-6	7.455208514218624E-5
18-19	9.940278018958166E-5	9.03661638087106E-6	1.3893797685589254E-4	9.03661638087106E-6	7.624645071359957E-5
20-21	1.1295770476088825E-4	1.0166193428479943E-5	1.558816325700258E-4	9.601404904675501E-6	8.019997038023066E-5
22-23	1.2877178342741261E-4	1.0166193428479943E-5	1.7282528828415904E-4	1.0166193428479943E-5	8.471827857066619E-5
24-25	1.6209430633187463E-4	1.0166193428479943E-5	1.8299148171263899E-4	1.0730981952284385E-5	8.471827857066619E-5
26-27	2.067125997124255E-4	1.0166193428479943E-5	2.0219429152198997E-4	1.5814078666524356E-5	8.471827857066619E-5
28-29	2.614970865214563E-4	1.0166193428479943E-5	2.078421767600344E-4	2.0897175380764327E-5	8.471827857066619E-5
30-31	3.145872077590738E-4	1.0166193428479943E-5	2.123604849504699E-4	2.146196390456877E-5	8.697743266588395E-5
32-33	3.834914076632156E-4	1.0166193428479943E-5	2.2026752428373208E-4	2.202675242837321E-5	9.31901064277328E-5
34-35	4.394054715198553E-4	1.0166193428479943E-5	2.451182193311275E-4	2.315632947598209E-5	1.0053235723719055E-4
36-37	5.049209402811705E-4	1.0730981952284385E-5	2.451182193311275E-4	2.3721117999786532E-5	1.1578164737991046E-4
38-39	6.25785684375321E-4	1.1295770476088824E-5	2.5584920128341186E-4	2.4850695047395416E-5	1.423267079987192E-4
40-41	7.246236760410981E-4	1.1295770476088824E-5	2.6206187504526076E-4	2.59802720950043E-5	1.4684501618915472E-4
42-43	8.104715316593732E-4	1.4684501618915473E-5	2.733576455213496E-4	2.710984914261318E-5	1.491041702843725E-4
44-45	9.070503692299326E-4	1.4684501618915473E-5	2.8239426190222066E-4	2.710984914261318E-5	1.6604782599850574E-4
46-47	0.0010363869411811498	1.4684501618915473E-5	3.010322831877672E-4	2.710984914261318E-5	1.6604782599850574E-4
48-49	0.0012086474409415044	1.6943655714133236E-5	3.027266487591805E-4	2.710984914261318E-5	1.8016753909361676E-4
50-51	0.0013425023210831568	1.7508444237937678E-5	3.038562258067894E-4	2.8239426190222062E-5	1.846858472840523E-4
52-53	0.0014791811438438316	1.807323276174212E-5	3.095041110448338E-4	2.8804214714026504E-5	1.8581542433166115E-4
54-55	0.0016429698157471198	1.807323276174212E-5	3.281421323303804E-4	3.1628157333048705E-5	1.9654640628394556E-4
56-57	0.001803369756507581	1.807323276174212E-5	3.693716945681046E-4	3.388731142826647E-5	2.1687879314090543E-4
58-59	0.0019462612530301046	1.863802128554656E-5	3.7389000275854013E-4	3.388731142826647E-5	2.2535062099797204E-4
60-61	0.0021111795019810014	2.315632947598209E-5	4.1681393056767764E-4	3.4452099952070913E-5	2.3043371771221203E-4
62-63	0.0022664963460272227	2.767463766641762E-5	4.908012271860595E-4	3.55816769996798E-5	2.394703340930831E-4
64-65	0.0024636075408349728	2.8239426190222062E-5	5.105688255192149E-4	3.8405619618702005E-5	2.394703340930831E-4
66-67	0.0026759680257854427	2.8239426190222062E-5	5.122631910906282E-4	4.292392780913754E-5	2.422942767121053E-4
68-69	0.002846534159974384	3.1628157333048705E-5	5.14522345185846E-4	4.292392780913754E-5	2.479421619501497E-4
70-71	0.003010322831877672	3.5016888475875355E-5	5.150871337096505E-4	4.348871633294198E-5	2.5584920128341186E-4
72-73	0.0032384973954946664	3.727604257109312E-5	5.241237500905215E-4	4.51830819043553E-5	2.620618750452607E-4
74-75	0.0034768381525401406	4.009998519011533E-5	5.241237500905215E-4	4.51830819043553E-5	2.631914520928696E-4
76-77	0.003654181749014735	4.574787042815974E-5	5.263829041857392E-4	4.51830819043553E-5	2.756167996165673E-4
78-79	0.0037778704357279078	4.687744747576862E-5	5.263829041857392E-4	4.51830819043553E-5	2.823942619022206E-4
80-81	0.0038738844847746624	4.7442235999573065E-5	5.320307894237837E-4	4.51830819043553E-5	2.8691257009265614E-4
82-83	0.003949001358440654	4.8007024523377506E-5	5.331603664713925E-4	4.51830819043553E-5	3.128928421876605E-4
84-85	0.004058005543534911	4.857181304718195E-5	5.342899435190014E-4	4.51830819043553E-5	3.2079988152092264E-4
86-87	0.004111660453296332	4.857181304718195E-5	5.47844868090308E-4	4.51830819043553E-5	3.337900175684248E-4
88-89	0.004120697069677204	4.913660157098639E-5	5.540575418521568E-4	4.51830819043553E-5	3.6372380933006015E-4
90	0.004120697069677204	4.970139009479083E-5	5.625293697092235E-4	4.51830819043553E-5	3.7163084866332236E-4
>>END_MODULE
