##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290297_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74837690
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.986380846335585	37.0	37.0	37.0	37.0	37.0
2	36.127768962403835	37.0	37.0	37.0	37.0	37.0
3	36.25817992244282	37.0	37.0	37.0	37.0	37.0
4	36.291246282454736	37.0	37.0	37.0	37.0	37.0
5	36.310640520839165	37.0	37.0	37.0	37.0	37.0
6	36.27443240164147	37.0	37.0	37.0	37.0	37.0
7	36.24271255566547	37.0	37.0	37.0	37.0	37.0
8	36.29900673043222	37.0	37.0	37.0	37.0	37.0
9	36.28172137595375	37.0	37.0	37.0	37.0	37.0
10-11	36.30303429996303	37.0	37.0	37.0	37.0	37.0
12-13	36.2856189641877	37.0	37.0	37.0	37.0	37.0
14-15	36.279197948253085	37.0	37.0	37.0	37.0	37.0
16-17	36.27808387057377	37.0	37.0	37.0	37.0	37.0
18-19	36.272032454502536	37.0	37.0	37.0	37.0	37.0
20-21	36.266803064071055	37.0	37.0	37.0	37.0	37.0
22-23	36.25354221916791	37.0	37.0	37.0	37.0	37.0
24-25	36.22234815104528	37.0	37.0	37.0	37.0	37.0
26-27	36.175179698892364	37.0	37.0	37.0	37.0	37.0
28-29	36.160029926097394	37.0	37.0	37.0	37.0	37.0
30-31	36.149146031899164	37.0	37.0	37.0	37.0	37.0
32-33	36.14242464592373	37.0	37.0	37.0	37.0	37.0
34-35	36.129112630280275	37.0	37.0	37.0	37.0	37.0
36-37	36.16603226968345	37.0	37.0	37.0	37.0	37.0
38-39	36.15713145526108	37.0	37.0	37.0	37.0	37.0
40-41	36.153634920728564	37.0	37.0	37.0	37.0	37.0
42-43	36.13641628428292	37.0	37.0	37.0	37.0	37.0
44-45	36.13664787980728	37.0	37.0	37.0	37.0	37.0
46-47	36.13091411480539	37.0	37.0	37.0	37.0	37.0
48-49	36.10244311065098	37.0	37.0	37.0	37.0	37.0
50-51	36.09488259045288	37.0	37.0	37.0	37.0	37.0
52-53	36.09263468714868	37.0	37.0	37.0	37.0	37.0
54-55	36.08177397501248	37.0	37.0	37.0	37.0	37.0
56-57	36.067713824037824	37.0	37.0	37.0	37.0	37.0
58-59	36.057310163369465	37.0	37.0	37.0	37.0	37.0
60-61	36.05323647782936	37.0	37.0	37.0	37.0	37.0
62-63	36.041979583848246	37.0	37.0	37.0	37.0	37.0
64-65	36.02442747612272	37.0	37.0	37.0	37.0	37.0
66-67	36.020156439063314	37.0	37.0	37.0	37.0	37.0
68-69	36.00642243520521	37.0	37.0	37.0	37.0	37.0
70-71	35.981792317148404	37.0	37.0	37.0	37.0	37.0
72-73	35.957656207569244	37.0	37.0	37.0	37.0	37.0
74-75	35.952909134358066	37.0	37.0	37.0	37.0	37.0
76-77	35.93816031199425	37.0	37.0	37.0	37.0	37.0
78-79	35.92579516403462	37.0	37.0	37.0	37.0	37.0
80-81	35.896846337757445	37.0	37.0	37.0	37.0	37.0
82-83	35.90914818660747	37.0	37.0	37.0	37.0	37.0
84-85	35.88856025084887	37.0	37.0	37.0	37.0	37.0
86-87	35.90306992513156	37.0	37.0	37.0	37.0	37.0
88-89	35.878461276975045	37.0	37.0	37.0	37.0	37.0
90-91	35.86169963100214	37.0	37.0	37.0	37.0	37.0
92-93	35.84816985015493	37.0	37.0	37.0	37.0	37.0
94-95	35.81566207581032	37.0	37.0	37.0	37.0	37.0
96-97	35.81382943024922	37.0	37.0	37.0	37.0	37.0
98-99	35.79160201780387	37.0	37.0	37.0	37.0	37.0
100-101	35.768578762292194	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107121.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	8.0
15	63.0
16	412.0
17	1034.0
18	1694.0
19	2603.0
20	4111.0
21	7675.0
22	14784.0
23	31358.0
24	59887.0
25	103228.0
26	157932.0
27	240117.0
28	351538.0
29	509995.0
30	692883.0
31	948312.0
32	1257424.0
33	1813729.0
34	2891630.0
35	6740045.0
36	4.4322171E7
37	1.4577936E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.878224435381874	16.907768908415356	10.935562976442956	46.27844367975982
2	21.700293860722205	19.402841320108088	32.14847970683893	26.74838511233078
3	23.10290374898997	19.52555078822513	23.23927744409379	34.13226801869111
4	24.536710004175134	24.77366792162388	20.967146657213327	29.72247541698766
5	25.78977440671552	28.613199402562934	23.111446698993205	22.48557949172834
6	21.812389786567795	32.90238697366268	23.22995158781676	22.05527165195276
7	19.00235498006177	22.705840765109123	37.14844992013911	21.14335433469
8	19.478236543334763	24.42391814252077	29.704916337516448	26.392928976628028
9	20.353464476014597	22.744985268076054	31.69202855618715	25.209521699722192
10-11	22.248806241526143	30.727036214591113	24.105961912319977	22.918195631562767
12-13	21.77917831158577	26.17349475405665	27.26708360322796	24.78024333112962
14-15	21.852958274577478	26.683422699907027	26.87634929437876	24.587269731136736
16-17	22.59788429672574	26.594057107651047	26.174477111035337	24.63358148458788
18-19	22.49963610868359	26.86798049803482	25.927996454723406	24.704386938558184
20-21	22.63753535752923	26.73254887322048	26.236886102278902	24.39302966697139
22-23	22.531645566232527	26.73861650623507	26.23583635691909	24.493901570613314
24-25	22.358391462476703	26.722998098971228	26.321900419087058	24.596710019465007
26-27	22.45018124760581	26.838516496292243	26.3415384049207	24.369763851181244
28-29	22.385366828250692	26.75964672642226	26.20422023282569	24.650766212501356
30-31	22.379726410135337	26.57686857814459	26.28075430180229	24.762650709917782
32-33	22.605360097836684	26.66751267841554	26.189405403880418	24.537721819867365
34-35	22.591026471009393	26.657558685009935	26.009337549352225	24.742077294628448
36-37	22.470997038479954	26.557450753887245	26.141319654800217	24.830232552832584
38-39	22.59740574881527	26.74143340907821	26.144470067497956	24.51669077460857
40-41	22.769970473749247	26.5859784192295	26.01479362911213	24.62925747790912
42-43	22.68843901259473	26.455371789890748	26.239490651955883	24.61669854555864
44-45	22.777965593356463	26.44260228345209	26.275037978192888	24.50439414499856
46-47	22.77687659826473	26.48898294715341	26.106158382948998	24.627982071632857
48-49	22.651233644325497	26.38358201575912	26.181398390304278	24.783785949611108
50-51	22.779798749176738	26.477097913331228	26.09838854768496	24.644714789807075
52-53	23.050851761799358	26.527896133154965	25.861015037936706	24.560237067108975
54-55	22.834815363456208	26.460541325326194	26.055176455577406	24.649466855640195
56-57	22.781221616759957	26.544314500890305	26.128623334612573	24.54584054773717
58-59	22.90578834653845	26.46182797019353	26.038379725197323	24.594003958070694
60-61	22.847935287683615	26.323411432839027	26.077308171567992	24.751345107909366
62-63	22.95543961629382	26.272917605937046	26.177234198721955	24.594408579047176
64-65	23.058812422765016	26.377704904810507	25.953224452511936	24.610258219912538
66-67	23.039165944831595	26.356772495604968	25.99252643369132	24.611535125872113
68-69	23.05057006275198	26.413933403597667	25.994265502540525	24.541231031109824
70-71	23.288426545872685	26.353456628385025	25.80130149532876	24.556815330413528
72-73	23.05259578916616	26.399906172290017	25.92111542544465	24.626382613099178
74-75	23.106300044503865	26.384045436502884	25.927580491463946	24.582074027529295
76-77	23.21875656166138	26.33662034164143	25.77886631820594	24.66575677849125
78-79	22.981582978247427	26.28601333395546	25.969101807519003	24.76330188027811
80-81	23.111610989813403	26.382523995918827	25.887718412884897	24.618146601382872
82-83	23.21975588913816	26.360251199014673	25.752545326404174	24.66744758544299
84-85	23.2082515523839	26.28829980285106	25.851682674867476	24.651765969897564
86-87	23.263588631360765	26.279247319496356	25.882374475958986	24.57478957318389
88-89	23.391917934221663	26.22129823272306	25.764177553277623	24.622606279777653
90-91	23.23424617804328	26.188360699253217	25.90712082938974	24.670272293313765
92-93	23.35603373807411	26.26674654097562	25.889693645435223	24.487526075515042
94-95	23.40616540686431	26.20130498804889	25.82754455103487	24.564985054051927
96-97	23.212170465970484	26.12414688434565	25.885288990902865	24.778393658781
98-99	23.432145382896326	25.327436932619364	26.34745354496496	24.89296413951935
100-101	24.20016887179532	11.954337792404255	32.48387173845999	31.361621597340438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107177.0
1	53613.5
2	42.5
3	46.0
4	71.5
5	98.5
6	110.5
7	121.0
8	155.0
9	196.5
10	245.5
11	341.0
12	521.0
13	793.0
14	1121.0
15	1594.5
16	2306.0
17	3275.0
18	4542.5
19	6075.5
20	8016.5
21	10416.0
22	13490.0
23	17978.0
24	24106.0
25	31627.5
26	42089.0
27	58013.0
28	81247.5
29	109887.0
30	147915.0
31	202131.5
32	280778.0
33	383239.0
34	516781.5
35	705759.5
36	943092.5
37	1219059.0
38	1569901.5
39	1986498.0
40	2410642.0
41	2838384.0
42	3288459.0
43	3680512.5
44	4004393.5
45	4233750.5
46	4360103.5
47	4398818.5
48	4271791.5
49	3996510.0
50	3678130.0
51	3366127.5
52	3042361.0
53	2696586.0
54	2320583.5
55	2003914.0
56	1727930.0
57	1457874.5
58	1234759.0
59	1060796.5
60	917910.5
61	806448.5
62	717672.5
63	632471.5
64	572856.5
65	511617.5
66	442853.0
67	388247.0
68	321611.0
69	250020.5
70	202329.0
71	157642.5
72	119692.0
73	95796.0
74	84262.5
75	65526.5
76	40445.0
77	34349.5
78	25082.5
79	15559.0
80	14425.0
81	10649.5
82	4305.0
83	582.5
84	390.0
85	299.5
86	229.5
87	174.5
88	144.5
89	100.5
90	84.0
91	79.5
92	69.5
93	65.0
94	72.0
95	115.0
96	252.5
97	689.0
98	2095.5
99	2447.5
100	918.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17883502283408267
2	0.15304587835354083
3	0.1449390006559529
4	0.14313776921762283
5	0.1431644937196752
6	0.14313776921762283
7	0.14313776921762283
8	0.14313776921762283
9	0.1431431141180333
10-11	0.14313776921762283
12-13	0.14315848070671344
14-15	0.1432359817626653
16-17	0.1431437822305846
18-19	0.14334020732066957
20-21	0.14316716616988046
22-23	0.1439261420281679
24-25	0.14732817113943522
26-27	0.1504121786762793
28-29	0.15333316675060388
30-31	0.15572033289643225
32-33	0.1583099371453074
34-35	0.16093628758450454
36-37	1.224643718351325E-4
38-39	1.8738125883853975E-5
40-41	2.6100161806949273E-5
42-43	3.5804940093918164E-4
44-45	8.365860244141769E-5
46-47	1.7401420917040694E-4
48-49	1.492542767742127E-4
50-51	6.626253259589514E-5
52-53	1.0106966714083959E-4
54-55	2.744346597481109E-5
56-57	6.693716331359896E-7
58-59	6.693913124387297E-7
60-61	0.0
62-63	2.6777321986103964E-6
64-65	2.0083639656257796E-6
66-67	8.703192922981269E-6
68-69	0.0
70-71	4.017134309752282E-6
72-73	3.3477374423623393E-6
74-75	0.0
76-77	2.6783998794505785E-6
78-79	0.0
80-81	1.339319538083156E-6
82-83	7.567512558755875E-5
84-85	1.1385322663526853E-5
86-87	4.688326013974574E-6
88-89	2.5519367009315183E-4
90-91	5.291875184411803E-5
92-93	5.359852238521518E-6
94-95	5.648710058426827E-4
96-97	2.6832908340314582E-5
98-99	1.8429035942660716E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	121710.0
36-37	1569.0
38-39	1792.0
40-41	1777.0
42-43	1950.0
44-45	1898.0
46-47	1846.0
48-49	1772.0
50-51	1979.0
52-53	1897.0
54-55	2055.0
56-57	2191.0
58-59	2208.0
60-61	2411.0
62-63	2353.0
64-65	2435.0
66-67	2486.0
68-69	2559.0
70-71	2796.0
72-73	2827.0
74-75	2985.0
76-77	3014.0
78-79	3499.0
80-81	3583.0
82-83	3571.0
84-85	3999.0
86-87	4164.0
88-89	5282.0
90-91	13404.0
92-93	9333.0
94-95	29348.0
96-97	522598.0
98-99	6741468.0
100-101	6.7328931E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.667403623615755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.069670766186675	15.789696157549512
2	17.08407939006218	9.453442400454911
3	8.206078295332064	6.811226410918348
4	4.594405652053329	5.084611023439239
5	2.863727158775214	3.96159475848721
6	1.931089213153842	3.205693481612274
7	1.3710492871285966	2.6553361810400267
8	1.0228257839471984	2.2639147040886676
9	0.7892330030811288	1.9652425244391571
>10	4.498741824477279	22.81310158671774
>50	0.3377666542802518	6.437064820279738
>100	0.20507030761516545	10.806235058936474
>500	0.016793646779180098	3.185090283494359
>1k	0.009090745512946094	4.558437182289951
>5k	3.0595498309049745E-4	0.565743856580908
>10k+	7.231663236684485E-5	0.44356956967165984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	107121	0.14313776921762283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.34490041047499E-6	0.0	4.008675307856242E-6
2	0.0	1.3362251026187474E-6	5.34490041047499E-6	4.008675307856242E-6	1.6034701231424966E-5
3	1.3362251026187474E-6	4.008675307856242E-6	5.34490041047499E-6	4.008675307856242E-6	2.2715826744518706E-5
4	6.681125513093737E-6	4.008675307856242E-6	2.2715826744518706E-5	8.017350615712483E-6	2.4052051847137453E-5
5	8.017350615712483E-6	4.008675307856242E-6	2.4052051847137453E-5	8.017350615712483E-6	2.9396952257612442E-5
6	2.4052051847137453E-5	4.008675307856242E-6	4.275920328379992E-5	9.35357571833123E-6	3.875052797594367E-5
7	2.6724502052374948E-5	4.008675307856242E-6	4.6767878591656156E-5	9.35357571833123E-6	4.0086753078562416E-5
8	3.073317736023119E-5	4.008675307856242E-6	4.6767878591656156E-5	9.35357571833123E-6	4.142297818118117E-5
9	4.0086753078562416E-5	4.008675307856242E-6	5.07765538995124E-5	1.068980082094998E-5	4.6767878591656156E-5
10-11	4.7435991142965534E-5	8.017350615712483E-6	6.146635472046238E-5	1.068980082094998E-5	5.278089155344052E-5
12-13	5.545334175867801E-5	8.017350615712483E-6	8.819085677283732E-5	1.2026025923568727E-5	6.480691747700924E-5
14-15	7.950539360581545E-5	8.017350615712483E-6	1.0756612076080916E-4	1.6034701231424966E-5	8.217784381105295E-5
16-17	1.0222122035033417E-4	1.2026025923568727E-5	1.2894572240270912E-4	2.4052051847137453E-5	9.019519442676544E-5
18-19	1.1758780903044975E-4	1.3362251026187474E-5	1.5901078721163093E-4	2.4052051847137453E-5	9.086330697807482E-5
20-21	1.2360082199223412E-4	1.3362251026187474E-5	1.8373095161007774E-4	2.6056389501065572E-5	9.353575718331231E-5
22-23	1.316181726079466E-4	1.3362251026187474E-5	2.0644677835459648E-4	2.9396952257612442E-5	9.954877014509668E-5
24-25	1.476528738393716E-4	1.3362251026187474E-5	2.1179167876507146E-4	3.206940246284993E-5	1.0088499524771542E-4
26-27	1.7036870058389028E-4	1.7370926334043714E-5	2.224814795860214E-4	3.3405627565468684E-5	1.0422555800426229E-4
28-29	1.9976565284150272E-4	1.7370926334043714E-5	2.3918429336875575E-4	4.0754865629871795E-5	1.1224290861997478E-4
30-31	2.3116694275304326E-4	1.870715143666246E-5	2.532146569462526E-4	4.409542838641866E-5	1.1892403413306851E-4
32-33	2.5521899460018073E-4	2.2715826744518706E-5	2.705855832802963E-4	4.476354093772803E-5	1.242689345435435E-4
34-35	2.912970723708869E-4	3.073317736023119E-5	3.1534912421802435E-4	4.543165348903741E-5	1.369630730184216E-4
36-37	3.2136213717980875E-4	3.206940246284993E-5	3.4073740116778055E-4	4.543165348903741E-5	1.5099343659591844E-4
38-39	3.561039898478961E-4	3.3405627565468684E-5	3.547677647452774E-4	4.8104103694274906E-5	1.6435568762210594E-4
40-41	3.9953130568300545E-4	3.54099652193968E-5	3.7080246597670237E-4	5.07765538995124E-5	1.697005880325809E-4
42-43	4.3427315835109287E-4	4.142297818118117E-5	3.855009421055086E-4	5.3449004104749895E-5	1.8172661395614965E-4
44-45	4.730236863270366E-4	5.144466645082177E-5	3.935182927212211E-4	5.612145430998739E-5	2.0377432814935897E-4
46-47	5.137785519569083E-4	5.2112779002131144E-5	4.2959637049192726E-4	5.745767941260613E-5	2.1446412897030895E-4
48-49	5.819260321904645E-4	5.278089155344052E-5	4.3560938345371165E-4	5.745767941260613E-5	2.2983071765042453E-4
50-51	6.327025860899769E-4	5.478522920736864E-5	4.569889850956116E-4	5.745767941260613E-5	2.5789144480541825E-4
52-53	6.968413910156768E-4	5.545334175867801E-5	4.997481883794115E-4	5.745767941260613E-5	2.732580334855338E-4
54-55	7.516266202230454E-4	5.612145430998739E-5	5.18455339816074E-4	5.812579196391551E-5	2.792710464473182E-4
56-57	8.144292000461265E-4	6.013012961784363E-5	5.39834941457974E-4	6.0798242169153E-5	2.866202845117213E-4
58-59	8.799042300744451E-4	6.013012961784363E-5	5.52529079932852E-4	6.280257982308112E-5	2.959738602300525E-4
60-61	9.433749224488357E-4	6.146635472046238E-5	5.886071577035582E-4	6.34706923743905E-5	2.9797819788398064E-4
62-63	0.001015531077990248	6.34706923743905E-5	6.413880492569988E-4	6.413880492569987E-5	3.0065064808921816E-4
64-65	0.0010883553460829696	6.547503002831862E-5	6.641038760015174E-4	6.681125513093735E-5	3.1134044891016816E-4
66-67	0.0011645201769322384	6.547503002831862E-5	6.647719885528268E-4	6.948370533617485E-5	3.166853493206431E-4
68-69	0.001219305406139607	6.614314257962799E-5	6.8281102743818E-4	7.683294340057797E-5	3.3071571289813997E-4
70-71	0.0012827760985139975	6.948370533617485E-5	6.854834776434174E-4	7.750105595188734E-5	3.460823015782556E-4
72-73	0.0013676263925302878	6.948370533617485E-5	6.961732784643674E-4	7.750105595188734E-5	3.547677647452774E-4
74-75	0.0014477998986874127	7.015181788748423E-5	6.975095035669861E-4	7.816916850319672E-5	3.5810832750182427E-4
76-77	0.0015172836040235876	7.61648308492686E-5	6.995138412209142E-4	8.017350615712483E-5	3.6946624087408363E-4
78-79	0.0015787499587440498	7.883728105450609E-5	7.021862914261517E-4	8.017350615712483E-5	3.955226303751492E-4
80-81	0.0016335351879514185	8.418218146498108E-5	7.048587416313892E-4	8.418218146498109E-5	4.035399809908617E-4
82-83	0.0016729538284786715	8.418218146498108E-5	7.155485424523392E-4	8.618651911890921E-5	4.135616692605023E-4
84-85	0.0017297433953399684	8.485029401629046E-5	7.208934428628141E-4	8.819085677283732E-5	4.2157901987621477E-4
86-87	0.0017544635597384153	8.88589693241467E-5	7.309151311324548E-4	8.952708187545607E-5	4.289282579406179E-4
88-89	0.0017598084601488902	9.086330697807482E-5	7.38264369196858E-4	9.62082073885498E-5	4.389499462102585E-4
90	0.0017598084601488902	9.086330697807482E-5	7.416049319534047E-4	9.754443249116856E-5	4.4229050896680536E-4
>>END_MODULE
