##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290299_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76940795
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.061157686244336	37.0	37.0	37.0	37.0	37.0
2	36.17732837306919	37.0	37.0	37.0	37.0	37.0
3	36.30071694736193	37.0	37.0	37.0	37.0	37.0
4	36.333300364260595	37.0	37.0	37.0	37.0	37.0
5	36.352306276533795	37.0	37.0	37.0	37.0	37.0
6	36.3203717741674	37.0	37.0	37.0	37.0	37.0
7	36.285293607896826	37.0	37.0	37.0	37.0	37.0
8	36.343719024478496	37.0	37.0	37.0	37.0	37.0
9	36.32720422241543	37.0	37.0	37.0	37.0	37.0
10-11	36.34559969129511	37.0	37.0	37.0	37.0	37.0
12-13	36.3266682388712	37.0	37.0	37.0	37.0	37.0
14-15	36.32116823071558	37.0	37.0	37.0	37.0	37.0
16-17	36.3201202236083	37.0	37.0	37.0	37.0	37.0
18-19	36.315607071645154	37.0	37.0	37.0	37.0	37.0
20-21	36.309731722943596	37.0	37.0	37.0	37.0	37.0
22-23	36.29634734733375	37.0	37.0	37.0	37.0	37.0
24-25	36.26510758044546	37.0	37.0	37.0	37.0	37.0
26-27	36.219759439709456	37.0	37.0	37.0	37.0	37.0
28-29	36.20575441805612	37.0	37.0	37.0	37.0	37.0
30-31	36.19573227700597	37.0	37.0	37.0	37.0	37.0
32-33	36.189480684466545	37.0	37.0	37.0	37.0	37.0
34-35	36.17703662276949	37.0	37.0	37.0	37.0	37.0
36-37	36.1799892662993	37.0	37.0	37.0	37.0	37.0
38-39	36.171274784849984	37.0	37.0	37.0	37.0	37.0
40-41	36.16852221257084	37.0	37.0	37.0	37.0	37.0
42-43	36.15340875204507	37.0	37.0	37.0	37.0	37.0
44-45	36.15323415560944	37.0	37.0	37.0	37.0	37.0
46-47	36.14851575028958	37.0	37.0	37.0	37.0	37.0
48-49	36.11914286108032	37.0	37.0	37.0	37.0	37.0
50-51	36.11253771404342	37.0	37.0	37.0	37.0	37.0
52-53	36.111677362179975	37.0	37.0	37.0	37.0	37.0
54-55	36.102011753416065	37.0	37.0	37.0	37.0	37.0
56-57	36.088434280625364	37.0	37.0	37.0	37.0	37.0
58-59	36.077891065906286	37.0	37.0	37.0	37.0	37.0
60-61	36.0730111578615	37.0	37.0	37.0	37.0	37.0
62-63	36.061236247548976	37.0	37.0	37.0	37.0	37.0
64-65	36.0448962181626	37.0	37.0	37.0	37.0	37.0
66-67	36.04100033278462	37.0	37.0	37.0	37.0	37.0
68-69	36.026187606561024	37.0	37.0	37.0	37.0	37.0
70-71	36.000757742900085	37.0	37.0	37.0	37.0	37.0
72-73	35.977473477612605	37.0	37.0	37.0	37.0	37.0
74-75	35.9737550130792	37.0	37.0	37.0	37.0	37.0
76-77	35.96055277430192	37.0	37.0	37.0	37.0	37.0
78-79	35.94792180288094	37.0	37.0	37.0	37.0	37.0
80-81	35.91985587700696	37.0	37.0	37.0	37.0	37.0
82-83	35.93166542088153	37.0	37.0	37.0	37.0	37.0
84-85	35.913839676903926	37.0	37.0	37.0	37.0	37.0
86-87	35.928819622549675	37.0	37.0	37.0	37.0	37.0
88-89	35.90375700429952	37.0	37.0	37.0	37.0	37.0
90-91	35.886473036829436	37.0	37.0	37.0	37.0	37.0
92-93	35.870565609809645	37.0	37.0	37.0	37.0	37.0
94-95	35.840333314646074	37.0	37.0	37.0	37.0	37.0
96-97	35.8398296782573	37.0	37.0	37.0	37.0	37.0
98-99	35.81827443952648	37.0	37.0	37.0	37.0	37.0
100-101	35.78988357034358	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37490.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	72.0
16	337.0
17	906.0
18	1685.0
19	2506.0
20	3983.0
21	6887.0
22	13183.0
23	27764.0
24	52387.0
25	93177.0
26	148892.0
27	236215.0
28	353687.0
29	518918.0
30	708851.0
31	968127.0
32	1278673.0
33	1833323.0
34	2906588.0
35	6790742.0
36	4.5795162E7
37	1.5161237E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.858570619412546	16.059638341565957	12.454291689198303	45.62749934982319
2	21.558722033420615	19.298171966610493	32.29896244166696	26.84414355830193
3	23.73812951395302	19.654559169999924	23.000291448974473	33.60701986707259
4	24.89543264481111	24.6723524996059	20.823015885286488	29.609198970296504
5	25.982114494753862	28.67553280790537	23.078402157598507	22.263950539742265
6	21.827726389652565	33.2194162526565	22.91370702468509	22.039150333005843
7	18.9536275456562	22.27115856724233	37.578122292663494	21.197091594437975
8	19.65903806084797	24.421955597356966	29.460235811711865	26.458770530083196
9	20.508438486486373	22.771134478550696	31.454746970887665	25.26568006407527
10-11	22.438578550037608	30.73159794627812	23.95155188713931	22.87827161654496
12-13	21.900576932479343	26.08496867753885	27.246209784550153	24.768244605431654
14-15	21.966495868905795	26.556345166919055	26.89773718615965	24.579421778015497
16-17	22.657175335223442	26.51327731462431	26.236867183409586	24.59268016674266
18-19	22.51230203248122	26.810240636969517	26.01052888005388	24.66692845049538
20-21	22.68133060838699	26.707035595149065	26.210925467842156	24.400708328621786
22-23	22.564214573497917	26.719336729213538	26.251434284533616	24.465014412754932
24-25	22.412583062707554	26.69485023014044	26.34476350119732	24.547803205954683
26-27	22.504247206280862	26.824484641766844	26.312904397803088	24.358363754149202
28-29	22.45362635973035	26.717247149004063	26.21977808150186	24.60934840976373
30-31	22.449694789046614	26.553640345026857	26.2839883849075	24.712676481019034
32-33	22.632907434001897	26.65877443285843	26.20674191995549	24.50157621318418
34-35	22.66656360561001	26.627657272081727	26.04615778039569	24.659621341912572
36-37	22.479143361748754	26.56788571034918	26.196690783644332	24.756280144257733
38-39	22.6327348223048	26.763547145784848	26.182968653648924	24.420749378261426
40-41	22.787457855338342	26.63378287855867	26.064375472803487	24.514383793299498
42-43	22.66569491482261	26.512391393500895	26.27401751519142	24.547896176485075
44-45	22.780243516683925	26.548091000170267	26.267242976747653	24.40442250639816
46-47	22.771161023849672	26.547107475402854	26.116389196646484	24.565342304100994
48-49	22.633202251562977	26.419632638149864	26.254219040160326	24.692946070126833
50-51	22.787339932260295	26.50329170028676	26.163239759774314	24.54612860767863
52-53	23.041090488499602	26.552948309093665	25.930614167426736	24.475347034979993
54-55	22.806881530653552	26.48105609662948	26.147786589090575	24.564275783626393
56-57	22.826965062100985	26.571626714511005	26.175893404224986	24.425514819163023
58-59	22.919994293860857	26.48153104465431	26.08097735246592	24.517497309018914
60-61	22.829338715058675	26.37761004554271	26.117776716683423	24.675274522715196
62-63	22.917603241924628	26.363444556366577	26.205905421072355	24.513046780636436
64-65	23.008995706077386	26.44748580130346	26.0189066597107	24.52461183290845
66-67	22.94552515099297	26.397232718398055	26.111911088902147	24.545331041706824
68-69	23.010906583655277	26.467621503031264	26.080110828150598	24.44136108516286
70-71	23.200637761060015	26.42261467722194	25.913202770310384	24.46354479140766
72-73	23.000230601551262	26.421221041882465	26.028894579308638	24.54965377725764
74-75	23.059815163993505	26.470723984530004	26.033834950909284	24.435625900567203
76-77	23.161005003342485	26.422663346199933	25.891329850778355	24.525001799679227
78-79	22.932603307483866	26.368627015361056	26.06237660551826	24.636393071636817
80-81	23.090634249335814	26.443373842503348	25.999131125537957	24.466860782622884
82-83	23.181575091608224	26.43606935449026	25.863008624276752	24.519346929624763
84-85	23.091881430638175	26.374303653430044	26.010286476542372	24.523528439389413
86-87	23.189973055452068	26.411406928796634	25.97981529175661	24.41880472399469
88-89	23.290498390537042	26.309260114356757	25.873406487180517	24.526835007925683
90-91	23.15244573711894	26.27182878811714	26.021955678407537	24.55376979635638
92-93	23.24894221136132	26.35377762562305	26.018432176626433	24.3788479863892
94-95	23.330955698551662	26.2742289330924	25.915800213016265	24.479015155339678
96-97	23.166570623914044	26.21459835411084	25.986107521658642	24.632723500316473
98-99	23.3463617833407	25.39260113659263	26.472331227225826	24.78870585284084
100-101	24.10707103411016	11.988919751417166	32.69898559591373	31.205023618558947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37576.0
1	18815.5
2	52.0
3	61.0
4	73.5
5	83.5
6	99.0
7	101.5
8	111.0
9	133.5
10	187.5
11	247.0
12	341.0
13	506.5
14	766.0
15	1125.0
16	1627.5
17	2325.5
18	3239.0
19	4487.5
20	6130.5
21	8341.5
22	11199.0
23	15366.5
24	21482.0
25	29152.5
26	39483.5
27	55300.5
28	77652.0
29	106177.0
30	146866.5
31	202150.5
32	278112.0
33	379887.0
34	513929.0
35	699416.0
36	936228.0
37	1222991.5
38	1591559.0
39	2036117.5
40	2500191.5
41	2959549.5
42	3443997.0
43	3883231.5
44	4228047.5
45	4464124.5
46	4582288.5
47	4589934.0
48	4446983.5
49	4162129.5
50	3815914.0
51	3476487.0
52	3132571.0
53	2755934.0
54	2366336.0
55	2049014.0
56	1760224.0
57	1468447.0
58	1223927.5
59	1035886.5
60	890617.0
61	777785.0
62	692289.5
63	621356.5
64	571282.0
65	518930.0
66	456817.5
67	397211.5
68	325109.0
69	255069.5
70	203868.5
71	156585.5
72	118274.0
73	92466.0
74	76115.0
75	55719.0
76	33743.0
77	25610.0
78	17084.5
79	9723.5
80	8476.5
81	6383.0
82	2621.0
83	541.5
84	365.5
85	284.5
86	210.0
87	170.5
88	137.0
89	105.0
90	97.5
91	85.0
92	69.0
93	69.0
94	75.0
95	93.5
96	200.5
97	521.5
98	1572.5
99	1838.5
100	699.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08519797592421549
2	0.05882834977205526
3	0.05046217679450804
4	0.048728376149479606
5	0.048759568964682524
6	0.0487257767482127
7	0.0487257767482127
8	0.0487257767482127
9	0.0487257767482127
10-11	0.0487257767482127
12-13	0.04875826926404907
14-15	0.04881935519382143
16-17	0.04873812390423052
18-19	0.048932429148931984
20-21	0.04874787165898143
22-23	0.049265802361413084
24-25	0.051608512753214986
26-27	0.05383619963895616
28-29	0.05608143248324897
30-31	0.05801343747487923
32-33	0.06006631462542075
34-35	0.06220952096998738
36-37	1.333047059851563E-4
38-39	2.7962003486913856E-5
40-41	3.251462800599362E-5
42-43	3.498648165919214E-4
44-45	9.039466486265796E-5
46-47	1.9184908420059253E-4
48-49	1.424267289107458E-4
50-51	6.763794319518392E-5
52-53	9.755718372858561E-5
54-55	3.707271332073386E-5
56-57	6.504171518697317E-7
58-59	1.9513059788463995E-6
60-61	0.0
62-63	7.80569664424515E-6
64-65	2.601983033717589E-6
66-67	5.854659216842663E-6
68-69	0.0
70-71	1.3011283085432176E-6
72-73	5.204718053494261E-6
74-75	0.0
76-77	1.951929709918065E-6
78-79	0.0
80-81	1.3014116151507217E-6
82-83	5.0757770895963996E-5
84-85	7.8093202949182E-6
86-87	5.206524743917731E-6
88-89	2.5969247828528297E-4
90-91	4.491344057165827E-5
92-93	5.208399896412738E-6
94-95	5.710562416427678E-4
96-97	2.3468516333435466E-5
98-99	1.7914264836489637E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	48952.0
36-37	1397.0
38-39	1573.0
40-41	1622.0
42-43	1748.0
44-45	1726.0
46-47	1704.0
48-49	1712.0
50-51	1873.0
52-53	2004.0
54-55	2246.0
56-57	2066.0
58-59	2346.0
60-61	2294.0
62-63	2437.0
64-65	2547.0
66-67	2690.0
68-69	2761.0
70-71	2953.0
72-73	3171.0
74-75	3129.0
76-77	3405.0
78-79	3800.0
80-81	4102.0
82-83	4159.0
84-85	4523.0
86-87	4823.0
88-89	6031.0
90-91	14793.0
92-93	10728.0
94-95	31575.0
96-97	553571.0
98-99	6948512.0
100-101	6.9257822E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.08344198505229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.0388421662671	15.73763572885864
2	17.21733934493894	9.670443012611017
3	8.406734021264993	7.082700815098823
4	4.667395298869326	5.243061003884099
5	2.984011442114291	4.190065610867445
6	2.065020577951494	3.479573135930395
7	1.4742694336852014	2.8981792077863973
8	1.070729498606573	2.4055815804641454
9	0.810406366698354	2.0483100165141215
>10	4.70917113459959	23.986420876219473
>50	0.3385404724445515	6.5408227529478875
>100	0.1963128477299897	10.438037761340919
>500	0.014629944668281882	2.7965671433348516
>1k	0.006405529574278948	3.0572420690525317
>5k	1.759272046183505E-4	0.3235730883482911
>10k+	1.5993382238031863E-5	0.10178619674097703
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.198802533818373E-6	0.0	0.0	1.2997006334545932E-6	1.0397605067636745E-5
2	5.198802533818373E-6	1.2997006334545932E-6	5.198802533818373E-6	1.2997006334545932E-6	1.2997006334545933E-5
3	7.79820380072756E-6	7.79820380072756E-6	9.097904434182152E-6	1.2997006334545932E-6	2.859341393600105E-5
4	1.689610823490971E-5	7.79820380072756E-6	2.4694312035637273E-5	1.2997006334545932E-6	3.119281520291024E-5
5	2.2094910768728083E-5	7.79820380072756E-6	3.509191710327402E-5	1.2997006334545932E-6	3.379221646981942E-5
6	5.328772597163832E-5	7.79820380072756E-6	4.159042027054698E-5	3.89910190036378E-6	3.509191710327402E-5
7	5.5887127238547507E-5	7.79820380072756E-6	5.5887127238547507E-5	3.89910190036378E-6	4.289012090400158E-5
8	6.758443293963885E-5	7.79820380072756E-6	5.71868278720021E-5	3.89910190036378E-6	4.289012090400158E-5
9	7.018383420654804E-5	7.79820380072756E-6	5.71868278720021E-5	5.198802533818373E-6	4.9388624071274545E-5
10-11	7.538263674036641E-5	8.448054117454856E-6	6.0436079455638584E-5	7.79820380072756E-6	5.588712723854751E-5
12-13	9.162889465854882E-5	1.559640760145512E-5	8.318084054109396E-5	9.097904434182152E-6	7.018383420654803E-5
14-15	1.0917485321018583E-4	1.559640760145512E-5	1.0722530226000394E-4	9.097904434182152E-6	7.278323547345722E-5
16-17	1.2672081176182283E-4	1.8845659185091603E-5	1.4101751872982337E-4	9.097904434182152E-6	7.60324870570937E-5
18-19	1.351688658792777E-4	2.4694312035637273E-5	1.6116287854836957E-4	9.097904434182152E-6	7.73321876905483E-5
20-21	1.4426677031345985E-4	2.4694312035637273E-5	1.715604836160063E-4	1.169730570109134E-5	8.643009212473045E-5
22-23	1.501154231640055E-4	2.4694312035637273E-5	1.8195808868364305E-4	1.2997006334545933E-5	9.61778468756399E-5
24-25	1.5856347728146036E-4	2.859341393600105E-5	1.8845659185091603E-4	1.559640760145512E-5	9.61778468756399E-5
26-27	1.8585719058400685E-4	3.7691318370183205E-5	2.0015389755200736E-4	2.079521013527349E-5	1.0007694877600368E-4
28-29	2.12501053569826E-4	4.0940569953819686E-5	2.2159895800400814E-4	2.339461140218268E-5	1.0787515257673124E-4
30-31	2.4044461718909974E-4	4.548952217091076E-5	2.2549805990437194E-4	2.339461140218268E-5	1.156733563774588E-4
32-33	2.6513892922473703E-4	5.2637875654911026E-5	2.462932700396454E-4	2.339461140218268E-5	1.2217185954473174E-4
34-35	2.859341393600105E-4	5.71868278720021E-5	2.690380311251008E-4	2.339461140218268E-5	1.3126976397891392E-4
36-37	3.275245596305575E-4	5.78366781887294E-5	2.904830915771016E-4	2.339461140218268E-5	1.371184168294596E-4
38-39	3.8731078876946877E-4	5.848652850545669E-5	2.976314450611018E-4	2.4694312035637273E-5	1.5271482443091468E-4
40-41	4.3669941284074334E-4	5.978622913891129E-5	3.262248589971029E-4	2.729371330254646E-5	1.6376227981527874E-4
42-43	4.893372884956544E-4	7.6682337373821E-5	3.5026932071601283E-4	3.054296488618294E-5	1.7091063329927901E-4
44-45	5.354766609832924E-4	8.123128959091208E-5	3.5676782388328583E-4	3.379221646981942E-5	1.8195808868364305E-4
46-47	5.952628901222036E-4	8.967934370836694E-5	3.886104894029234E-4	3.379221646981942E-5	1.8975629248437062E-4
48-49	6.472509154603874E-4	8.967934370836694E-5	4.016074957374693E-4	3.509191710327402E-5	1.9625479565164356E-4
50-51	7.03138042698935E-4	9.292859529200342E-5	4.1330480143856067E-4	3.509191710327402E-5	2.0795210135273492E-4
52-53	7.551260680371186E-4	9.357844560873072E-5	4.412483650578344E-4	3.5741767420001314E-5	2.1834970642037167E-4
54-55	8.227105009767575E-4	9.357844560873072E-5	4.613937248763806E-4	4.678922280436536E-5	2.313467127549176E-4
56-57	8.824967301156689E-4	9.812739782582178E-5	4.834886356451087E-4	4.8738773754547244E-5	2.4564341972291813E-4
58-59	9.37734007037489E-4	1.0267635004291287E-4	4.919366897625636E-4	5.0688324704729135E-5	2.6383922859128244E-4
60-61	0.0010066181406105826	1.0722530226000394E-4	5.380760622502016E-4	5.3937576288365615E-5	2.7163743239201E-4
62-63	0.0010768019748171305	1.1177425447709501E-4	6.030610939229313E-4	5.458742660509292E-5	2.7228728270873724E-4
64-65	0.0011534843121909517	1.1307395511054962E-4	6.407524122931144E-4	5.523727692182021E-5	2.7943563619273757E-4
66-67	0.0012184693438636813	1.143736557440042E-4	6.453013645102055E-4	5.913637882218399E-5	2.885335406269197E-4
68-69	0.0013029498850382296	1.143736557440042E-4	6.543992689443878E-4	6.693458262291155E-5	3.060794991785567E-4
70-71	0.0013828814739956872	1.143736557440042E-4	6.55049119261115E-4	6.758443293963885E-5	3.281744099472848E-4
72-73	0.001474510368654236	1.143736557440042E-4	6.751944790796612E-4	6.758443293963885E-5	3.4377081754873994E-4
74-75	0.0015635398620458757	1.1762290732764068E-4	6.758443293963885E-4	6.823428325636614E-5	3.59367225150195E-4
76-77	0.0016558186070211518	1.1957245827782258E-4	6.777938803465704E-4	7.018383420654804E-5	3.691149799011045E-4
78-79	0.0017318510940782456	1.2802051239527742E-4	6.816929822469342E-4	7.018383420654804E-5	3.7691318370183205E-4
80-81	0.0018046343295517026	1.351688658792777E-4	6.868917847807526E-4	7.148353484000263E-5	3.879606390861961E-4
82-83	0.00185337310330625	1.3776826714618687E-4	6.946899885814801E-4	7.473278642363912E-5	4.00957645420742E-4
84-85	0.0019034115776942518	1.4101751872982337E-4	7.018383420654803E-4	7.6682337373821E-5	4.1265495112183335E-4
86-87	0.001928105889729889	1.4231721936327796E-4	7.09636545866208E-4	7.6682337373821E-5	4.2175285555601553E-4
88-89	0.0019326548419469802	1.4491662063018714E-4	7.109362464996625E-4	8.253099022436667E-5	4.470970179083801E-4
90	0.0019326548419469802	1.4556647094691444E-4	7.135356477665717E-4	8.318084054109396E-5	4.5619492234256223E-4
>>END_MODULE
