##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290293_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95191489
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.94298554359203	37.0	37.0	37.0	37.0	37.0
2	36.122066280526404	37.0	37.0	37.0	37.0	37.0
3	36.2581496125142	37.0	37.0	37.0	37.0	37.0
4	36.292182823193365	37.0	37.0	37.0	37.0	37.0
5	36.31115308008261	37.0	37.0	37.0	37.0	37.0
6	36.27322468923666	37.0	37.0	37.0	37.0	37.0
7	36.239405090091616	37.0	37.0	37.0	37.0	37.0
8	36.30271077070766	37.0	37.0	37.0	37.0	37.0
9	36.28596453617823	37.0	37.0	37.0	37.0	37.0
10-11	36.30760652877275	37.0	37.0	37.0	37.0	37.0
12-13	36.28898899774538	37.0	37.0	37.0	37.0	37.0
14-15	36.28394346263457	37.0	37.0	37.0	37.0	37.0
16-17	36.28352223800176	37.0	37.0	37.0	37.0	37.0
18-19	36.27924040562071	37.0	37.0	37.0	37.0	37.0
20-21	36.27261928847442	37.0	37.0	37.0	37.0	37.0
22-23	36.25961109296232	37.0	37.0	37.0	37.0	37.0
24-25	36.22835362413545	37.0	37.0	37.0	37.0	37.0
26-27	36.1818740223719	37.0	37.0	37.0	37.0	37.0
28-29	36.16917036564057	37.0	37.0	37.0	37.0	37.0
30-31	36.15789429452039	37.0	37.0	37.0	37.0	37.0
32-33	36.15155463110783	37.0	37.0	37.0	37.0	37.0
34-35	36.13859574672689	37.0	37.0	37.0	37.0	37.0
36-37	36.162031286931	37.0	37.0	37.0	37.0	37.0
38-39	36.1550447563383	37.0	37.0	37.0	37.0	37.0
40-41	36.154143178936465	37.0	37.0	37.0	37.0	37.0
42-43	36.137860126302506	37.0	37.0	37.0	37.0	37.0
44-45	36.13668114814952	37.0	37.0	37.0	37.0	37.0
46-47	36.13244230004264	37.0	37.0	37.0	37.0	37.0
48-49	36.10458774850234	37.0	37.0	37.0	37.0	37.0
50-51	36.09761604131906	37.0	37.0	37.0	37.0	37.0
52-53	36.09769942364145	37.0	37.0	37.0	37.0	37.0
54-55	36.08578202228182	37.0	37.0	37.0	37.0	37.0
56-57	36.070844645281426	37.0	37.0	37.0	37.0	37.0
58-59	36.059614218739256	37.0	37.0	37.0	37.0	37.0
60-61	36.05587540799914	37.0	37.0	37.0	37.0	37.0
62-63	36.04496244711538	37.0	37.0	37.0	37.0	37.0
64-65	36.02937618570239	37.0	37.0	37.0	37.0	37.0
66-67	36.02579561615678	37.0	37.0	37.0	37.0	37.0
68-69	36.010908767719386	37.0	37.0	37.0	37.0	37.0
70-71	35.9894802488586	37.0	37.0	37.0	37.0	37.0
72-73	35.965914583853895	37.0	37.0	37.0	37.0	37.0
74-75	35.961811450521125	37.0	37.0	37.0	37.0	37.0
76-77	35.948443361482916	37.0	37.0	37.0	37.0	37.0
78-79	35.93533037149546	37.0	37.0	37.0	37.0	37.0
80-81	35.90495685090618	37.0	37.0	37.0	37.0	37.0
82-83	35.91960658726512	37.0	37.0	37.0	37.0	37.0
84-85	35.90119058274208	37.0	37.0	37.0	37.0	37.0
86-87	35.91560250900214	37.0	37.0	37.0	37.0	37.0
88-89	35.88982960326925	37.0	37.0	37.0	37.0	37.0
90-91	35.87183651271128	37.0	37.0	37.0	37.0	37.0
92-93	35.859485748516065	37.0	37.0	37.0	37.0	37.0
94-95	35.8295822038271	37.0	37.0	37.0	37.0	37.0
96-97	35.8293423635808	37.0	37.0	37.0	37.0	37.0
98-99	35.807987886793896	37.0	37.0	37.0	37.0	37.0
100-101	35.78381277728208	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102748.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	12.0
15	76.0
16	354.0
17	953.0
18	1794.0
19	2733.0
20	4572.0
21	7901.0
22	15276.0
23	31741.0
24	60759.0
25	109943.0
26	176592.0
27	287502.0
28	434128.0
29	642431.0
30	881053.0
31	1212743.0
32	1618717.0
33	2347236.0
34	3764730.0
35	8810045.0
36	5.6724553E7
37	1.7952896E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.439906181378497	15.941302796290174	10.850227496386099	48.76856352594523
2	21.027821139559506	18.999300657061365	34.422705303924	25.550172899455127
3	22.80266077878451	19.92817255195471	23.799361191652515	33.46980547760827
4	24.38233957842621	25.633180661205117	20.92365133713311	29.06082842323557
5	25.696528733340074	29.583133831048876	23.10651802975703	21.61381940585402
6	21.155259590617568	33.79360338780803	23.266698840822805	21.7844381807516
7	18.588801170477165	21.971636999589677	38.385497185203036	21.05406464473013
8	19.30410983146785	23.9261565152072	30.11868040192056	26.651053251404388
9	20.363498349015877	22.194690980235933	32.11249828318263	25.32931238756555
10-11	22.432344132268742	30.788114182667087	23.995812627258427	22.783729057805747
12-13	21.696958149221505	25.95920813784003	27.600490072844707	24.74334364009376
14-15	21.804187528076433	26.540385702386445	27.061004608625645	24.594422160911485
16-17	22.63315704676434	26.52931681685866	26.34113574379905	24.496390392577947
18-19	22.536764726760723	26.833855159151526	25.984001104149623	24.645379009938125
20-21	22.64148073186566	26.65939734926254	26.365468126877907	24.333653791993893
22-23	22.520619545989632	26.802404416169175	26.361419228688142	24.31555680915305
24-25	22.390653652764833	26.756369598579948	26.4471766454817	24.405800103173515
26-27	22.471555526514333	26.90096686131823	26.480471062653077	24.14700654951436
28-29	22.353623065429076	26.822051636965828	26.33382229170588	24.49050300589922
30-31	22.433698078214327	26.538586992401385	26.42110667730494	24.606608252079347
32-33	22.66295541061384	26.722004374447632	26.278064390432053	24.336975824506474
34-35	22.656267334023	26.728145443925033	26.123344610417842	24.492242611634126
36-37	22.478362909106657	26.60310356491255	26.3169528034916	24.601580722489192
38-39	22.67509954347389	26.723855139148238	26.382142272420882	24.218903044956992
40-41	22.816809577738486	26.59742640002359	26.15141948552666	24.43434453671126
42-43	22.666798199424747	26.48187948060521	26.392021436214126	24.459300883755922
44-45	22.7552493533915	26.471011527268846	26.44836701539022	24.325372103949434
46-47	22.803789245858543	26.464626002369542	26.286015618432558	24.445569133339358
48-49	22.659138399913957	26.35007655376418	26.355637568736036	24.635147477585832
50-51	22.794936300439588	26.417333246835362	26.301709183165478	24.486021269559572
52-53	23.052271080421125	26.5640950751889	25.973072066962555	24.41056177742742
54-55	22.718558057229757	26.46208084573221	26.2509773798073	24.56838371723073
56-57	22.747757741814006	26.547494369763875	26.335883166798524	24.368864721623595
58-59	22.858363075552784	26.446110289157907	26.247544018511963	24.447982616777338
60-61	22.872759292953923	26.283502053745732	26.21419766287063	24.629540990429714
62-63	22.95283532508291	26.190786477142282	26.35974008428985	24.496638113484963
64-65	22.995569205613407	26.407674578305766	26.068323045341852	24.528433170738975
66-67	23.03401828567005	26.33037629538717	26.13701509753337	24.49859032140941
68-69	22.99385936057153	26.419172371498977	26.23943675464503	24.347531513284462
70-71	23.25888289286202	26.38879197951603	26.02737635377177	24.32494877385018
72-73	23.064225018678243	26.298727707869883	26.09966482044859	24.537382453003286
74-75	23.05802988745864	26.35574183091758	26.16197887664921	24.42424940497457
76-77	23.17826488122741	26.35502008895909	25.993722822430936	24.472992207382564
78-79	22.956507914799047	26.28775843512304	26.112806456722787	24.642927193355128
80-81	23.11715976016287	26.414359163312916	26.07189870213333	24.39658237439088
82-83	23.208307048964652	26.419722128936623	25.87188088767403	24.500089934424693
84-85	23.161002259999773	26.366802922924197	25.940821238384405	24.53137357869163
86-87	23.113527257330425	26.372422053294684	25.985359066156715	24.528691623218172
88-89	23.314529590287318	26.18348848493402	25.925422142255332	24.576559782523326
90-91	23.184849060935846	26.17087388320009	26.113043939179576	24.53123311668449
92-93	23.30045175241376	26.231100464290126	26.142229031583824	24.32621875171229
94-95	23.31475148602146	26.23525222071815	26.04303659709312	24.40695969616727
96-97	23.166983557011644	26.077946276520002	26.13429088710219	24.620779279366165
98-99	23.326023825090424	25.355641595201224	26.55927987518235	24.759054704526
100-101	24.1617166878409	11.924369138684558	32.659228906241964	31.254685267232585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102859.0
1	51471.0
2	93.0
3	124.5
4	157.0
5	177.5
6	213.0
7	236.5
8	245.0
9	250.0
10	311.0
11	400.0
12	491.5
13	655.5
14	903.0
15	1282.0
16	1875.5
17	2709.5
18	3914.5
19	5493.5
20	7295.0
21	9938.0
22	14059.0
23	19830.0
24	27599.0
25	37605.0
26	50874.0
27	72142.5
28	101670.0
29	137463.0
30	188722.0
31	258371.5
32	358452.5
33	490526.0
34	655130.5
35	875603.0
36	1171858.0
37	1547551.5
38	2008543.0
39	2552364.5
40	3081769.5
41	3618293.5
42	4222897.0
43	4757922.0
44	5192316.5
45	5511918.5
46	5667515.0
47	5697914.0
48	5524267.5
49	5193049.0
50	4813571.0
51	4399397.5
52	3951057.0
53	3436190.5
54	2931340.5
55	2551256.5
56	2229729.5
57	1882333.5
58	1548762.0
59	1265101.5
60	1050234.5
61	909494.0
62	821892.0
63	735391.5
64	645476.5
65	563541.5
66	495255.5
67	429388.0
68	348927.0
69	272476.5
70	215365.0
71	166929.0
72	127072.0
73	101222.0
74	88365.5
75	66518.0
76	39190.0
77	31490.0
78	23778.0
79	17026.5
80	13902.5
81	8524.5
82	3385.0
83	530.0
84	356.5
85	268.0
86	219.5
87	182.5
88	130.0
89	109.0
90	87.5
91	67.0
92	59.0
93	59.5
94	82.0
95	123.0
96	252.5
97	665.5
98	1908.0
99	2249.5
100	899.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14393303586206113
2	0.11791180196792593
3	0.10971674158810564
4	0.10794137278386307
5	0.10796133255148471
6	0.107938221241607
7	0.107938221241607
8	0.107938221241607
9	0.10793927175569236
10-11	0.10793874649864968
12-13	0.10795923152331402
14-15	0.10803171699520321
16-17	0.10794557484020445
18-19	0.10812626326288477
20-21	0.10797288820642358
22-23	0.10859636831607918
24-25	0.11139913989579467
26-27	0.1137244528237183
28-29	0.11603663432557505
30-31	0.11782145775658578
32-33	0.11963779661015703
34-35	0.12148617614333146
36-37	1.225357430977667E-4
38-39	2.4192018275870727E-5
40-41	3.0503561898293247E-5
42-43	3.5184906518699745E-4
44-45	8.046934222461009E-5
46-47	1.7198674618590463E-4
48-49	1.5884080713956817E-4
50-51	6.574655052964554E-5
52-53	1.109822110295815E-4
54-55	4.1553401649299743E-5
56-57	5.260034845942443E-7
58-59	5.260145105100434E-7
60-61	0.0
62-63	6.838480184811926E-6
64-65	6.312586732969028E-6
66-67	7.364857740644609E-6
68-69	0.0
70-71	1.0521714200834113E-6
72-73	7.365392054531763E-6
74-75	0.0
76-77	2.6306430891667048E-6
78-79	0.0
80-81	1.0523224675303402E-6
82-83	4.893457409033113E-5
84-85	8.945325167542389E-6
86-87	1.0524292245030025E-5
88-89	2.6259128638756135E-4
90-91	5.631057837421287E-5
92-93	6.842579814074789E-6
94-95	5.706288157413287E-4
96-97	2.7931454503141507E-5
98-99	2.0913977720800715E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	116835.0
36-37	1534.0
38-39	1817.0
40-41	1654.0
42-43	1933.0
44-45	1867.0
46-47	1701.0
48-49	1679.0
50-51	1707.0
52-53	1886.0
54-55	1972.0
56-57	2013.0
58-59	1942.0
60-61	2064.0
62-63	2142.0
64-65	2197.0
66-67	2268.0
68-69	2159.0
70-71	2363.0
72-73	2650.0
74-75	2641.0
76-77	2807.0
78-79	2992.0
80-81	3078.0
82-83	3079.0
84-85	3327.0
86-87	3613.0
88-89	4489.0
90-91	14855.0
92-93	9183.0
94-95	33444.0
96-97	682160.0
98-99	8529205.0
100-101	8.5742233E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.253351361734868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.10111253302149	12.039310662580851
2	16.916578723860916	7.52901140361051
3	8.712843923503275	5.816699315690249
4	4.914521579374921	4.374583019226332
5	3.1982024051416396	3.5585360923781213
6	2.172327139612505	2.900493546625773
7	1.5092210736639216	2.350965878434459
8	1.1974669471034185	2.1318122174365084
9	0.9330505602908363	1.8687151760774008
>10	5.545833717764205	23.019624372001363
>50	0.45431353020687837	6.993895399053778
>100	0.29499354911018666	12.748111104872928
>500	0.02953280102908519	4.524960542255671
>1k	0.018856649430115422	7.876601880341948
>5k	8.802514269356714E-4	1.2749222077588669
>10k+	2.646154596309687E-4	0.991757181655617
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	102748	0.107938221241607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.202056341402539E-6	0.0	1.0505140853506347E-6	0.0	1.0505140853506347E-5
2	5.252570426753174E-6	3.151542256051904E-6	1.0505140853506347E-6	0.0	2.2060795792363327E-5
3	7.353598597454443E-6	3.151542256051904E-6	1.0505140853506347E-6	0.0	2.521233804841523E-5
4	1.4707197194908885E-5	3.151542256051904E-6	6.303084512103808E-6	0.0	2.7313366219116502E-5
5	3.151542256051904E-5	3.151542256051904E-6	9.454626768155712E-6	0.0	2.941439438981777E-5
6	4.307107749937602E-5	3.151542256051904E-6	2.2060795792363327E-5	0.0	2.941439438981777E-5
7	4.4121591584726654E-5	5.252570426753174E-6	2.6262852133765866E-5	0.0	3.0464908475168406E-5
8	5.14751901821811E-5	5.252570426753174E-6	2.6262852133765866E-5	3.151542256051904E-6	3.2565936645869674E-5
9	5.4626732438233004E-5	5.252570426753174E-6	2.6262852133765866E-5	3.151542256051904E-6	3.991953524332412E-5
10-11	6.408135920638871E-5	7.87885564012976E-6	3.624273594459689E-5	4.727313384077856E-6	4.25458204567007E-5
12-13	7.301072893186911E-5	1.1030397896181665E-5	5.567724652358364E-5	5.252570426753174E-6	4.832364792612919E-5
14-15	9.559678176690776E-5	1.1555654938856981E-5	7.196021484651847E-5	5.252570426753174E-6	5.094993313950578E-5
16-17	1.0977872191914132E-4	1.3656683109558251E-5	9.191998246818053E-5	5.777827469428491E-6	5.567724652358364E-5
18-19	1.1713232051659576E-4	1.575771128025952E-5	1.034756374070375E-4	6.303084512103808E-6	5.882878877963554E-5
20-21	1.2343540502869957E-4	1.575771128025952E-5	1.1713232051659577E-4	7.87885564012976E-6	6.775815850511594E-5
22-23	1.30789003626154E-4	1.575771128025952E-5	1.397183733516344E-4	1.1555654938856981E-5	7.353598597454442E-5
24-25	1.4602145786373822E-4	1.785873945096079E-5	1.4707197194908884E-4	1.4707197194908885E-5	7.353598597454442E-5
26-27	1.6755699661342625E-4	2.3111309877713963E-5	1.612539121013224E-4	1.5232454237584201E-5	7.87885564012976E-5
28-29	1.9329459170451676E-4	2.3111309877713963E-5	1.7753688042425726E-4	1.995976762166206E-5	8.19400986573495E-5
30-31	2.242847572223605E-4	2.3111309877713963E-5	1.8909253536311422E-4	2.1010281707012695E-5	8.404112682805078E-5
32-33	2.4792132414274976E-4	2.521233804841523E-5	1.9224407761916614E-4	2.1535538749688012E-5	8.981895429747925E-5
34-35	2.694568628924378E-4	2.7313366219116502E-5	2.3426464103319154E-4	2.2586052835038645E-5	9.297049655353118E-5
36-37	3.0149754249563215E-4	2.8363880304467135E-5	2.4161823963064598E-4	2.3111309877713963E-5	1.0662717966308943E-4
38-39	3.419423347816316E-4	2.8363880304467135E-5	2.4949709527077575E-4	2.363656692038928E-5	1.1660706347392045E-4
40-41	3.634778735313196E-4	2.8889137347142452E-5	2.694568628924378E-4	2.41618239630646E-5	1.2816271841277743E-4
42-43	3.8238712706763103E-4	3.5192221859246264E-5	2.8626508825804796E-4	2.521233804841523E-5	1.2973848954080339E-4
44-45	4.0970049328674753E-4	3.93942782006488E-5	2.883661164287492E-4	2.521233804841523E-5	1.3446580292488124E-4
46-47	4.391148876765653E-4	4.4121591584726654E-5	2.9519445798352836E-4	2.521233804841523E-5	1.3866785926628376E-4
48-49	4.984689334988761E-4	4.517210567007729E-5	3.0412382770900876E-4	2.573759509109055E-5	1.4969825716246545E-4
50-51	5.315601271874211E-4	4.569736271275261E-5	3.156794826478657E-4	2.6788109176441184E-5	1.6808225365610156E-4
52-53	5.683281201746933E-4	4.6222619755427925E-5	3.309119368854499E-4	2.8889137347142456E-5	1.7123379591215345E-4
54-55	6.14025482887446E-4	4.6222619755427925E-5	3.4666964816570944E-4	3.099016551784372E-5	1.7438533816820534E-4
56-57	6.55520789258796E-4	4.674787679810324E-5	3.687304439580728E-4	3.151542256051904E-5	1.8383996493636105E-4
58-59	7.080464935263278E-4	4.727313384077856E-5	3.776598136835532E-4	3.151542256051904E-5	1.974966480459193E-4
60-61	7.674005393486386E-4	5.042467609683046E-5	4.17054091884202E-4	3.151542256051904E-5	1.9907241917394526E-4
62-63	8.267545851709495E-4	5.252570426753173E-5	4.68529282066383E-4	3.151542256051904E-5	2.0169870438732185E-4
64-65	8.714014337983515E-4	5.725301765160959E-5	4.958426482854995E-4	3.151542256051904E-5	2.090523029847763E-4
66-67	9.412606204741687E-4	5.9879302864986176E-5	4.974184194135255E-4	3.151542256051904E-5	2.1430487341152947E-4
68-69	9.927358106563498E-4	5.9879302864986176E-5	5.026709898402787E-4	3.2040679603194356E-5	2.2638578539306177E-4
70-71	0.001041584715625154	6.0404559907661494E-5	5.073983032243565E-4	3.2565936645869674E-5	2.3899195441726937E-4
72-73	0.0011214237861118025	6.092981695033681E-5	5.389137257848756E-4	3.2565936645869674E-5	2.53173894569503E-4
74-75	0.0011975860572997236	6.303084512103808E-5	5.399642398702262E-4	3.2565936645869674E-5	2.610527502096327E-4
76-77	0.00124958650452458	6.565713033441467E-5	5.436410391689534E-4	3.2565936645869674E-5	2.6735583472173655E-4
78-79	0.0012884555256825535	6.723290146244062E-5	5.525704088944338E-4	3.309119368854499E-5	2.726084051484897E-4
80-81	0.001342557001078111	6.828341554779126E-5	5.567724652358364E-4	3.361645073122031E-5	2.8416406008734666E-4
82-83	0.0013845775644921363	6.828341554779126E-5	5.59398750449213E-4	3.361645073122031E-5	2.915176586848011E-4
84-85	0.0014339517265036163	6.828341554779126E-5	5.630755497479402E-4	3.361645073122031E-5	3.035985706663334E-4
86-87	0.0014507599518692265	6.828341554779126E-5	5.69378634260044E-4	3.414170777389563E-5	3.1095216926378785E-4
88-89	0.001454962008210629	6.828341554779126E-5	5.709544053880699E-4	3.5192221859246264E-5	3.225078242026448E-4
90	0.001454962008210629	6.933392963314189E-5	5.725301765160959E-4	3.571747890192158E-5	3.309119368854499E-4
>>END_MODULE
