##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290288_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83113885
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.102067674973924	37.0	37.0	37.0	37.0	37.0
2	36.10569496300167	37.0	37.0	37.0	37.0	37.0
3	36.21295076027309	37.0	37.0	37.0	37.0	37.0
4	36.18315003564086	37.0	37.0	37.0	37.0	37.0
5	36.14373446747195	37.0	37.0	37.0	37.0	37.0
6	36.06023310064257	37.0	37.0	37.0	37.0	37.0
7	36.0147760268937	37.0	37.0	37.0	37.0	37.0
8	36.09455862880191	37.0	37.0	37.0	37.0	37.0
9	36.037627683990465	37.0	37.0	37.0	37.0	37.0
10-11	36.0741345444266	37.0	37.0	37.0	37.0	37.0
12-13	36.05717071822596	37.0	37.0	37.0	37.0	37.0
14-15	36.04916387917615	37.0	37.0	37.0	37.0	37.0
16-17	36.03107314138907	37.0	37.0	37.0	37.0	37.0
18-19	36.03554963168909	37.0	37.0	37.0	37.0	37.0
20-21	36.025656597570915	37.0	37.0	37.0	37.0	37.0
22-23	35.995650756789914	37.0	37.0	37.0	37.0	37.0
24-25	35.98301481154442	37.0	37.0	37.0	37.0	37.0
26-27	35.95476969943109	37.0	37.0	37.0	37.0	37.0
28-29	35.93557651648699	37.0	37.0	37.0	37.0	37.0
30-31	35.914325019219106	37.0	37.0	37.0	37.0	37.0
32-33	35.8887443536059	37.0	37.0	37.0	37.0	37.0
34-35	35.872649413512555	37.0	37.0	37.0	37.0	37.0
36-37	35.8634683602959	37.0	37.0	37.0	37.0	37.0
38-39	35.84739346962836	37.0	37.0	37.0	37.0	37.0
40-41	35.84847733234672	37.0	37.0	37.0	37.0	37.0
42-43	35.81194079464579	37.0	37.0	37.0	37.0	37.0
44-45	35.79814653214324	37.0	37.0	37.0	37.0	37.0
46-47	35.80033629887009	37.0	37.0	37.0	37.0	37.0
48-49	35.77296528663214	37.0	37.0	37.0	37.0	37.0
50-51	35.75263635711015	37.0	37.0	37.0	37.0	37.0
52-53	35.737357721375744	37.0	37.0	37.0	37.0	37.0
54-55	35.73016187660892	37.0	37.0	37.0	37.0	37.0
56-57	35.69833898871589	37.0	37.0	37.0	37.0	37.0
58-59	35.721398455587426	37.0	37.0	37.0	37.0	37.0
60-61	35.68488000936188	37.0	37.0	37.0	37.0	37.0
62-63	35.670603649759144	37.0	37.0	37.0	37.0	37.0
64-65	35.657606396730316	37.0	37.0	37.0	37.0	37.0
66-67	35.63752315504749	37.0	37.0	37.0	37.0	37.0
68-69	35.601856960927584	37.0	37.0	37.0	37.0	37.0
70-71	35.60340350567134	37.0	37.0	37.0	37.0	37.0
72-73	35.581529199495805	37.0	37.0	37.0	37.0	37.0
74-75	35.59241392711103	37.0	37.0	37.0	37.0	37.0
76-77	35.56481271666938	37.0	37.0	37.0	37.0	37.0
78-79	35.51838823243182	37.0	37.0	37.0	37.0	37.0
80-81	35.51307418644723	37.0	37.0	37.0	37.0	37.0
82-83	35.478652170333845	37.0	37.0	37.0	37.0	37.0
84-85	35.4329938746789	37.0	37.0	37.0	37.0	37.0
86-87	35.392121799686635	37.0	37.0	37.0	37.0	37.0
88-89	35.41584504846125	37.0	37.0	37.0	37.0	37.0
90-91	35.35693971753109	37.0	37.0	37.0	37.0	37.0
92-93	35.39561664718278	37.0	37.0	37.0	37.0	37.0
94-95	35.343885636781614	37.0	37.0	37.0	37.0	37.0
96-97	35.31554108442313	37.0	37.0	37.0	37.0	37.0
98-99	35.30746217604394	37.0	37.0	37.0	37.0	37.0
100-101	35.28282200682463	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25532.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	33.0
12	1389.0
13	10374.0
14	20962.0
15	22216.0
16	20541.0
17	21962.0
18	24200.0
19	29842.0
20	39864.0
21	60545.0
22	87243.0
23	122505.0
24	157358.0
25	200910.0
26	250469.0
27	330930.0
28	429288.0
29	579867.0
30	779971.0
31	1119937.0
32	1631930.0
33	2698342.0
34	4933950.0
35	1.2380704E7
36	4.7761889E7
37	9371132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.458702939434662	16.178540955721296	11.490561327419147	46.872194777424895
2	21.68543899877108	19.210361813570156	32.88133435077311	26.222864836885652
3	23.64334891666583	19.62551860109748	22.916417055929035	33.81471542630766
4	24.920725761581686	24.650796963218575	20.82569558285649	29.60278169234325
5	26.09549666711936	28.54287274905208	23.243861884844726	22.117768698983838
6	22.02887092870207	32.849601254028464	23.268744372848502	21.85278344442096
7	19.275156048070716	22.175661737563438	37.46836845540985	21.080813758955998
8	19.579346109659802	24.123568825429633	29.99148551137812	26.30559955353245
9	20.574082641259782	22.482683523229387	31.901337168191002	25.04189666731983
10-11	22.51722838399876	30.58672590098433	24.204414962242407	22.691630752774508
12-13	21.96845360967554	26.096703455967134	27.377929658439953	24.55691327591737
14-15	22.031526131168604	26.581083702680168	26.996799057600658	24.39059110855057
16-17	22.83867913057688	26.49248869769983	26.24896024675637	24.419871924966923
18-19	22.69271573365583	26.764284824268696	25.969834212155433	24.57316522992004
20-21	22.830043531335097	26.63603688598378	26.294441894914733	24.239477687766385
22-23	22.68211206461427	26.677493891775335	26.367437652160252	24.272956391450144
24-25	22.52224273163302	26.637527514929488	26.439221911988	24.40100784144949
26-27	22.616001964890984	26.77467938122977	26.413663401830412	24.19565525204883
28-29	22.525127843413863	26.710954421724082	26.285811352049798	24.47810638281226
30-31	22.539791341584483	26.535162343974466	26.338912503937035	24.586133810504016
32-33	22.751013295379092	26.62317750074992	26.239502317651368	24.386306886219618
34-35	22.771250383019783	26.622940668663013	26.078934907973235	24.52687404034397
36-37	22.60509823242983	26.511975581708853	26.25177803163942	24.6311481542219
38-39	22.734536001837217	26.716800479368896	26.259011174764986	24.2896523440289
40-41	22.901489371371337	26.577940618362845	26.12130098712574	24.39926902314008
42-43	22.798492014989318	26.460209553066587	26.30238192367319	24.438916508270903
44-45	22.880498467244067	26.470039036312908	26.352342224523255	24.297120271919766
46-47	22.855934525067575	26.500701268604203	26.18548826974079	24.457875936587428
48-49	22.752722310689638	26.35292885338554	26.276337090803086	24.618011745121738
50-51	22.900382626917587	26.436282820218576	26.20054859548115	24.462785957382685
52-53	23.166235554743466	26.490459131112836	25.950761462297113	24.392543851846586
54-55	22.922438308106937	26.425771569978163	26.17018887266813	24.481601249246765
56-57	22.907801707086183	26.5573789283923	26.213595120552213	24.321224243969304
58-59	23.009950516845294	26.462530566101705	26.090976721686843	24.436542195366158
60-61	22.956983124694787	26.321555632444714	26.129359562022884	24.592101680837608
62-63	23.053658379564943	26.272085651702852	26.25569541915429	24.418560549577904
64-65	23.153191111731342	26.41613793609577	26.020825144002263	24.409845808170626
66-67	23.155199162486365	26.35548797790004	26.0505647725945	24.438748087019096
68-69	23.152193040186408	26.448109655182822	26.070073450464776	24.329623854165995
70-71	23.380372909621343	26.389402409949174	25.898810332232493	24.33141434819699
72-73	23.162686218186764	26.397628909454628	25.994945804784606	24.444739067574
74-75	23.19484370430514	26.459763179086526	26.001948752080605	24.34344436452773
76-77	23.31006072837894	26.41734231947483	25.85316225196614	24.419434700180094
78-79	23.10686103888097	26.370460737180355	26.00749570443786	24.515182519500815
80-81	23.249838267670203	26.433852181842678	25.941425996439442	24.37488355404768
82-83	23.28859190902565	26.42731565200222	25.83651481726771	24.447577621704415
84-85	23.29944015271997	26.334925073945843	25.934269123693852	24.431365649640334
86-87	23.35165794417472	26.33963926491783	25.95094231130827	24.35776047959918
88-89	23.464028314428404	26.257645750196968	25.83911127909282	24.43921465628181
90-91	23.329863496883927	26.2068233807777	25.97710392370044	24.48620919863793
92-93	23.440907816479957	26.30072437839668	25.958917691504684	24.29945011361868
94-95	23.525571155859073	26.2346767973963	25.871265332077588	24.36848671466704
96-97	23.365705583819935	26.150727270632874	25.9239779246544	24.559589220892793
98-99	23.566606071847048	25.368787971703554	26.389189480362024	24.675416476087374
100-101	24.384850141237738	11.944816852129179	32.56471207067643	31.105620935956658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25667.0
1	12903.0
2	254.5
3	577.5
4	1005.5
5	1400.0
6	1758.5
7	2065.0
8	2342.5
9	2716.5
10	3042.5
11	3327.0
12	3641.5
13	3965.0
14	4372.0
15	4862.5
16	5455.0
17	6217.0
18	7137.5
19	8274.5
20	9900.0
21	12093.5
22	15163.5
23	19676.0
24	26110.5
25	34260.0
26	44890.5
27	61585.0
28	85980.5
29	116023.0
30	157307.5
31	215023.0
32	296556.5
33	405069.5
34	548500.0
35	749138.5
36	1007251.5
37	1316572.5
38	1715928.5
39	2206216.5
40	2712080.0
41	3194808.5
42	3701039.5
43	4153868.5
44	4510584.5
45	4758153.5
46	4889952.5
47	4939119.5
48	4803462.0
49	4485047.0
50	4125470.5
51	3771585.0
52	3402626.0
53	3009749.0
54	2596591.0
55	2254417.0
56	1942377.5
57	1613559.0
58	1339741.0
59	1134651.5
60	972375.0
61	843928.0
62	744538.0
63	664987.0
64	605890.0
65	548290.0
66	485839.0
67	426105.5
68	350580.0
69	272417.0
70	214927.0
71	163748.5
72	122849.5
73	95807.0
74	80046.0
75	59716.0
76	36128.5
77	26569.0
78	19009.5
79	13122.0
80	11176.0
81	8467.5
82	5022.5
83	3100.5
84	2721.0
85	2479.5
86	2342.0
87	2203.0
88	2034.5
89	1874.5
90	1711.5
91	1574.0
92	1456.5
93	1393.0
94	1332.5
95	1254.0
96	1237.5
97	1265.0
98	1440.0
99	1986.5
100	10258.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07766211385738978
2	0.04656021072772618
3	0.03149894870153164
4	0.03134013047278442
5	0.03128358155800321
6	0.03221603706769332
7	0.03073613993618515
8	0.03140269522956363
9	0.03072170191538995
10-11	0.030757796967377956
12-13	0.030742757362382953
14-15	0.03073132726258675
16-17	0.03159821009449865
18-19	0.030784266672169158
20-21	0.030772836572372956
22-23	0.0329012414712656
24-25	0.03488105507280763
26-27	0.037795128936639166
28-29	0.04058888596051069
30-31	0.04314020455186278
32-33	0.046207682386643335
34-35	0.04872832018380563
36-37	2.0825390894392757E-4
38-39	5.375006596120217E-4
40-41	6.873928160781445E-4
42-43	0.0011581247771219264
44-45	9.095516387769903E-4
46-47	6.441071062230138E-4
48-49	5.941603894253609E-4
50-51	0.0010529032914286653
52-53	3.9010960183445426E-4
54-55	4.033664166441073E-5
56-57	8.428811295339238E-5
58-59	1.8062310926739604E-5
60-61	4.7565661400130407E-5
62-63	4.816937440669255E-6
64-65	1.746200615196111E-5
66-67	8.430245376803756E-6
68-69	1.3248009089194078E-5
70-71	1.987272662782171E-5
72-73	6.022276061905529E-7
74-75	8.431514759387665E-6
76-77	3.55342986837885E-5
78-79	4.8786489380419E-5
80-81	2.7887922187227934E-4
82-83	0.0016010720653258606
84-85	1.7469294191480164E-4
86-87	0.001334965268131509
88-89	5.771526380640985E-4
90-91	0.0022768739312617964
92-93	0.0010413367731756324
94-95	0.0011445434316804126
96-97	0.0018621029271419284
98-99	0.0017488652578656382
100-101	3.542827161255012E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	41755.0
36-37	1934.0
38-39	2157.0
40-41	2167.0
42-43	2417.0
44-45	2212.0
46-47	2304.0
48-49	2255.0
50-51	2426.0
52-53	2604.0
54-55	2576.0
56-57	2545.0
58-59	2752.0
60-61	2780.0
62-63	2824.0
64-65	3021.0
66-67	3065.0
68-69	2938.0
70-71	3240.0
72-73	3234.0
74-75	3430.0
76-77	3560.0
78-79	3819.0
80-81	4012.0
82-83	3961.0
84-85	4388.0
86-87	4565.0
88-89	5718.0
90-91	15776.0
92-93	10536.0
94-95	33886.0
96-97	592948.0
98-99	7536953.0
100-101	7.4799127E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.398227959103473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.90399119096661	16.13864978351126
2	15.791832769527206	8.653364682230933
3	7.66879907216694	6.303345154553732
4	4.377174685934445	4.797073194481962
5	2.841397166032199	3.8924623638650373
6	1.8991748747297679	3.1220415691228776
7	1.4052766145642843	2.6951462322000608
8	1.02947221728138	2.2564571589311155
9	0.7803069894208586	1.924112589681098
>10	4.709044666851861	23.699510155582768
>50	0.3501849358860497	6.6333624893933685
>100	0.21656985686807226	11.327318106071063
>500	0.017343646783588327	3.2685836144571407
>1k	0.009039555398014052	4.3807984689237305
>5k	3.063745242913376E-4	0.5438907519410594
>10k+	8.53830641467662E-5	0.3638836850530231
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.203168399600139E-6	1.203168399600139E-6	3.609505198800417E-6	0.0	2.406336799200278E-6
2	1.203168399600139E-6	3.609505198800417E-6	4.812673598400556E-6	0.0	1.082851559640125E-5
3	2.406336799200278E-6	3.609505198800417E-6	7.219010397600834E-6	0.0	2.0453862793202364E-5
4	1.3234852395601528E-5	3.609505198800417E-6	9.625347196801112E-6	0.0	2.526653639160292E-5
5	2.526653639160292E-5	3.609505198800417E-6	1.3234852395601528E-5	2.406336799200278E-6	2.6469704791203057E-5
6	3.368871518880389E-5	3.609505198800417E-6	2.6469704791203057E-5	2.406336799200278E-6	2.6469704791203057E-5
7	3.489188358840403E-5	4.812673598400556E-6	3.0079209990003475E-5	2.406336799200278E-6	2.6469704791203057E-5
8	4.090772558640473E-5	4.812673598400556E-6	3.368871518880389E-5	2.406336799200278E-6	2.6469704791203057E-5
9	4.090772558640473E-5	7.219010397600834E-6	3.609505198800417E-5	3.609505198800417E-6	3.729822038760431E-5
10-11	5.71504989810066E-5	7.219010397600834E-6	4.93299043836057E-5	3.609505198800417E-6	4.331406238560501E-5
12-13	7.158851977620827E-5	7.219010397600834E-6	5.835366738060674E-5	9.625347196801112E-6	5.354099378220619E-5
14-15	8.602654057140993E-5	7.219010397600834E-6	6.858059877720794E-5	1.203168399600139E-5	6.136158837960709E-5
16-17	1.1129307696301286E-4	8.422178797200973E-6	9.324555096901078E-5	1.203168399600139E-5	6.376792517880737E-5
18-19	1.2332476095901424E-4	9.625347196801112E-6	9.86598087672114E-5	1.3234852395601528E-5	6.557267777820757E-5
20-21	1.3836436595401598E-4	9.625347196801112E-6	1.1369941376221314E-4	1.3234852395601528E-5	6.978376717680806E-5
22-23	1.582166445474183E-4	1.082851559640125E-5	1.317469397562152E-4	1.3836436595401597E-5	7.339327237560848E-5
24-25	1.9250694393602223E-4	1.082851559640125E-5	1.3415327655541548E-4	2.0453862793202364E-5	7.339327237560848E-5
26-27	2.189766487272253E-4	1.7445941794202016E-5	1.3836436595401598E-4	2.286019959240264E-5	7.579960917480875E-5
28-29	2.544701165154294E-4	2.1055446993002434E-5	1.515992183496175E-4	2.7672873190803197E-5	8.362020377220965E-5
30-31	3.044016050988352E-4	2.2860199592402638E-5	1.6423248654541897E-4	2.7672873190803197E-5	8.903446157041029E-5
32-33	3.5252834108284075E-4	3.0680794189803546E-5	1.6784199174421942E-4	2.7672873190803197E-5	8.903446157041029E-5
34-35	3.8621705627164463E-4	3.489188358840403E-5	2.0213229113282336E-4	2.8274457390603264E-5	9.264396676921071E-5
36-37	4.5058656565025206E-4	3.609505198800417E-5	2.0874971733062414E-4	2.8876041590403335E-5	1.1008990856341272E-4
38-39	5.179639960278598E-4	3.669663618780424E-5	2.286019959240264E-4	3.0079209990003475E-5	1.2573109775821452E-4
40-41	5.853414264054676E-4	3.729822038760431E-5	2.50860611316629E-4	3.1282378389603616E-5	1.3956753435361612E-4
42-43	6.545236093824756E-4	4.211089398600487E-5	2.7311922670923157E-4	3.1883962589403686E-5	1.4077070275321627E-4
44-45	7.200962871606832E-4	4.812673598400556E-5	2.8154140550643253E-4	3.54934677882041E-5	1.5400555514881779E-4
46-47	8.001069857340924E-4	4.993148858340577E-5	3.098158628970358E-4	3.8501388787204446E-5	1.6182614974621869E-4
48-49	8.891414473045027E-4	5.053307278320584E-5	3.3147289408983827E-4	4.391564658540508E-5	1.774673389410205E-4
50-51	9.793790772745132E-4	5.053307278320584E-5	3.477156674844402E-4	4.752515178420549E-5	1.9431169653542246E-4
52-53	0.0010696167072445236	5.2939409582406114E-5	3.6816953027764255E-4	4.872832018380563E-5	2.0092912273322322E-4
54-55	0.0011556432478159336	5.3540993782206184E-5	3.8381071947244435E-4	5.4142577982006254E-5	2.051402121318237E-4
56-57	0.0012428729567869436	5.4744162181806325E-5	4.0065507706684626E-4	5.5345746381606395E-5	2.1657031192802502E-4
58-59	0.0013613850441475573	5.6548914781206536E-5	4.0727250326464705E-4	5.5345746381606395E-5	2.2980516432362656E-4
60-61	0.0014991478259017733	5.6548914781206536E-5	4.421643868530511E-4	5.71504989810066E-5	2.3702417472122738E-4
62-63	0.0016134488238637866	5.895525158040681E-5	4.818689440398557E-4	5.895525158040681E-5	2.3943051152042766E-4
64-65	0.0017379767532224007	6.196317257940716E-5	5.131513224294593E-4	6.136158837960709E-5	2.5146219551642904E-4
66-67	0.0018570904247828147	6.497109357840751E-5	5.173624118280598E-4	6.136158837960709E-5	2.568764533146297E-4
68-69	0.0019894389487388297	6.497109357840751E-5	5.36613106221662E-4	6.196317257940716E-5	2.652986321118306E-4
70-71	0.0021193811358956446	6.797901457740785E-5	5.378162746212621E-4	6.256475677920723E-5	2.7372081090903163E-4
72-73	0.0022463154020534596	7.158851977620827E-5	5.534574638160639E-4	6.436950937860744E-5	2.809398213066324E-4
74-75	0.002407539967599878	7.219010397600834E-5	5.570669690148644E-4	6.677584617780771E-5	2.881588317042333E-4
76-77	0.002527856807559892	7.219010397600834E-5	5.612780584134649E-4	6.737743037760778E-5	2.9778417890103444E-4
78-79	0.0026241102795279032	7.219010397600834E-5	5.636843952126652E-4	6.737743037760778E-5	3.194412100938369E-4
80-81	0.0027149494936977137	7.339327237560848E-5	5.654891478120653E-4	6.797901457740785E-5	3.260586362916377E-4
82-83	0.0027769126662771206	7.339327237560848E-5	5.721065740098661E-4	7.339327237560848E-5	3.344808150888386E-4
84-85	0.002873166138245132	7.459644077520862E-5	5.781224160078668E-4	7.459644077520862E-5	3.489188358840403E-4
86-87	0.002919488121629737	7.88075301738091E-5	5.847398422056675E-4	7.459644077520862E-5	3.765917090748435E-4
88-89	0.002926105547827538	8.121386697300938E-5	5.88349347404468E-4	8.181545117280946E-5	4.042645822656467E-4
90	0.002926105547827538	8.181545117280946E-5	5.907556842036682E-4	8.422178797200974E-5	4.06670919064847E-4
>>END_MODULE
