##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290298_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71746246
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03077317801408	37.0	37.0	37.0	37.0	37.0
2	36.16276245310451	37.0	37.0	37.0	37.0	37.0
3	36.293852141058366	37.0	37.0	37.0	37.0	37.0
4	36.32852032425502	37.0	37.0	37.0	37.0	37.0
5	36.346792778537846	37.0	37.0	37.0	37.0	37.0
6	36.311043674675325	37.0	37.0	37.0	37.0	37.0
7	36.27654283124444	37.0	37.0	37.0	37.0	37.0
8	36.33581899183966	37.0	37.0	37.0	37.0	37.0
9	36.32098395782268	37.0	37.0	37.0	37.0	37.0
10-11	36.33948715588548	37.0	37.0	37.0	37.0	37.0
12-13	36.32176397075884	37.0	37.0	37.0	37.0	37.0
14-15	36.31665835868263	37.0	37.0	37.0	37.0	37.0
16-17	36.31671505851331	37.0	37.0	37.0	37.0	37.0
18-19	36.310551669839285	37.0	37.0	37.0	37.0	37.0
20-21	36.30403566341297	37.0	37.0	37.0	37.0	37.0
22-23	36.29122410111883	37.0	37.0	37.0	37.0	37.0
24-25	36.25936558408924	37.0	37.0	37.0	37.0	37.0
26-27	36.21446575058436	37.0	37.0	37.0	37.0	37.0
28-29	36.20089005771814	37.0	37.0	37.0	37.0	37.0
30-31	36.19084703748821	37.0	37.0	37.0	37.0	37.0
32-33	36.184140512383046	37.0	37.0	37.0	37.0	37.0
34-35	36.17075129617235	37.0	37.0	37.0	37.0	37.0
36-37	36.172805937576754	37.0	37.0	37.0	37.0	37.0
38-39	36.16514165804665	37.0	37.0	37.0	37.0	37.0
40-41	36.16397126037039	37.0	37.0	37.0	37.0	37.0
42-43	36.14904721336481	37.0	37.0	37.0	37.0	37.0
44-45	36.14739308196576	37.0	37.0	37.0	37.0	37.0
46-47	36.140049029817156	37.0	37.0	37.0	37.0	37.0
48-49	36.11109712564913	37.0	37.0	37.0	37.0	37.0
50-51	36.10604302885183	37.0	37.0	37.0	37.0	37.0
52-53	36.10675445104347	37.0	37.0	37.0	37.0	37.0
54-55	36.09628642065012	37.0	37.0	37.0	37.0	37.0
56-57	36.080000136730405	37.0	37.0	37.0	37.0	37.0
58-59	36.06782951624264	37.0	37.0	37.0	37.0	37.0
60-61	36.06479849224737	37.0	37.0	37.0	37.0	37.0
62-63	36.05553888260937	37.0	37.0	37.0	37.0	37.0
64-65	36.03931440864894	37.0	37.0	37.0	37.0	37.0
66-67	36.03351750427605	37.0	37.0	37.0	37.0	37.0
68-69	36.01559014834786	37.0	37.0	37.0	37.0	37.0
70-71	35.99201020246722	37.0	37.0	37.0	37.0	37.0
72-73	35.970484058820475	37.0	37.0	37.0	37.0	37.0
74-75	35.96769102278749	37.0	37.0	37.0	37.0	37.0
76-77	35.95285365059876	37.0	37.0	37.0	37.0	37.0
78-79	35.93708975903907	37.0	37.0	37.0	37.0	37.0
80-81	35.906303974686914	37.0	37.0	37.0	37.0	37.0
82-83	35.92142738081027	37.0	37.0	37.0	37.0	37.0
84-85	35.905944584466354	37.0	37.0	37.0	37.0	37.0
86-87	35.91947790613054	37.0	37.0	37.0	37.0	37.0
88-89	35.891232923968104	37.0	37.0	37.0	37.0	37.0
90-91	35.87316656326139	37.0	37.0	37.0	37.0	37.0
92-93	35.85993388806075	37.0	37.0	37.0	37.0	37.0
94-95	35.83038044381571	37.0	37.0	37.0	37.0	37.0
96-97	35.83129443858516	37.0	37.0	37.0	37.0	37.0
98-99	35.80652446175	37.0	37.0	37.0	37.0	37.0
100-101	35.776697707421064	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35138.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.0
15	56.0
16	321.0
17	975.0
18	1804.0
19	2816.0
20	4227.0
21	6892.0
22	13058.0
23	25513.0
24	47773.0
25	83604.0
26	133924.0
27	214311.0
28	323815.0
29	479675.0
30	658303.0
31	908460.0
32	1204535.0
33	1742119.0
34	2780702.0
35	6500261.0
36	4.2819693E7
37	1.3758265E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.993022606917638	16.549237077107072	11.836950894177274	45.620789421798015
2	21.902866537728556	19.87503599337539	32.03686474734446	26.185232721551593
3	23.363776800994387	20.20537905684396	23.324289839208188	33.10655430295347
4	24.62655576316015	25.62270129754515	20.63658255008415	29.114160389210554
5	25.687752816565983	29.486769437204437	22.80765969177094	22.017818054458637
6	21.36243802006239	33.68905972000879	23.048747203850205	21.89975505607862
7	18.726803105594183	22.391637011102937	37.76244399961021	21.119115883692665
8	19.6036059016129	24.375388259235933	29.41143232649536	26.60957351265581
9	20.523793064912425	22.797693288958616	31.335499134541305	25.343014511587647
10-11	22.441543505366003	30.983077963319154	23.716426191602004	22.858952339712836
12-13	21.862007763038946	26.19956965653235	27.192167844829363	24.74625473559934
14-15	21.93521402010322	26.739356806706045	26.7947576088878	24.530671564302942
16-17	22.645594904281456	26.692496093021354	26.154410662499426	24.507498340197763
18-19	22.519672461538047	26.98463104684789	25.92131322171884	24.574383269895222
20-21	22.639360520042352	26.87948267968204	26.147742923836077	24.33341387643953
22-23	22.541974833946206	26.915064648628817	26.155369229899712	24.387591287525268
24-25	22.38147125178885	26.885490680247777	26.246165621981238	24.48687244598214
26-27	22.48788192337667	26.991302767244534	26.236778469497086	24.28403683988171
28-29	22.42637325115097	26.89816342380468	26.137629461029306	24.537833864015045
30-31	22.415634282415006	26.753469865769276	26.21658670537211	24.614309146443617
32-33	22.61621975531437	26.852412740708942	26.12518698530338	24.406180518673317
34-35	22.63967932327186	26.822248983202723	25.950328010447997	24.587743683077417
36-37	22.471061432081093	26.730534750723773	26.110392843849787	24.688010973345346
38-39	22.613220552145723	26.90568678625695	26.093402619640095	24.38769004195723
40-41	22.75753924331386	26.76630016827467	25.977675353355366	24.4984852350561
42-43	22.667143984257113	26.636542758527632	26.19545056229922	24.500862694916034
44-45	22.764789194739002	26.648614723906896	26.208066058162444	24.378530023191658
46-47	22.777119371207043	26.655176267201103	26.04914372309294	24.51856063849892
48-49	22.615562056669095	26.55747323456393	26.15896620043002	24.667998508336957
50-51	22.78935204873827	26.63199996423438	26.079776197632203	24.498871789395142
52-53	23.028721607101982	26.688111577555446	25.847459985526854	24.435706829815718
54-55	22.78271401791186	26.605233992885346	26.067337087905422	24.54471490129737
56-57	22.79182643059265	26.698638017202825	26.09964392005294	24.409891632151588
58-59	22.904224998810893	26.593007047501814	26.012055158869863	24.490712794817433
60-61	22.83175291109135	26.49152819203725	26.056931054532374	24.61978784233903
62-63	22.920221399287854	26.445912533341158	26.135992817202087	24.4978732501689
64-65	23.033958635026988	26.546737294210487	25.922900760461935	24.496403310300582
66-67	22.997610809158807	26.513509687058452	25.984588730219905	24.50429077356284
68-69	23.028750171336775	26.577522663334737	25.975442724181054	24.418284441147435
70-71	23.22920574362213	26.53406168243614	25.814638276003627	24.4220942979381
72-73	23.012923047625193	26.54634328423342	25.93796245329504	24.502771214846348
74-75	23.036269576820985	26.55718726448406	25.96164668489141	24.444896473803546
76-77	23.16073822404348	26.502874850153823	25.82539626190358	24.51099066389911
78-79	22.953658881852043	26.443904544797135	25.99924509129321	24.603191482057603
80-81	23.102055666194165	26.52562956462768	25.916087868048116	24.456226901130044
82-83	23.203236676080266	26.520589891337465	25.778431425897168	24.4977420066851
84-85	23.141658559177635	26.459171472402076	25.893684231152957	24.505485737267335
86-87	23.1990585860424	26.46738497759789	25.895918394022775	24.43763804233693
88-89	23.335880991585665	26.371099226319792	25.806290147566806	24.48672963452773
90-91	23.183832926957823	26.35155735975393	25.94837038891196	24.516239324376286
92-93	23.29833735428321	26.41324131990436	25.91410302781309	24.374318297999338
94-95	23.346224493012553	26.358348220078465	25.84155425054814	24.45387303636085
96-97	23.1854613309096	26.26681281552244	25.91477450735763	24.63295134621033
98-99	23.38567375947564	25.44143881290652	26.395338180403638	24.777549247214207
100-101	24.156873900330353	12.035628623370362	32.58862292089864	31.21887455540063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35242.0
1	17643.5
2	47.0
3	49.5
4	71.5
5	89.5
6	91.0
7	103.0
8	124.0
9	152.5
10	184.0
11	232.5
12	332.5
13	517.0
14	769.0
15	1123.5
16	1644.5
17	2322.5
18	3279.5
19	4569.0
20	6122.5
21	8149.0
22	11187.0
23	15718.0
24	22060.5
25	30104.5
26	41113.0
27	58521.5
28	83454.5
29	114293.0
30	156239.0
31	213489.0
32	295159.5
33	400375.0
34	533418.0
35	716587.0
36	946312.0
37	1216795.0
38	1555715.0
39	1954451.0
40	2359379.5
41	2768332.5
42	3196425.0
43	3565027.5
44	3861915.0
45	4068343.0
46	4176570.0
47	4199907.0
48	4072335.5
49	3812078.5
50	3499360.5
51	3187577.0
52	2876443.5
53	2544082.5
54	2193612.5
55	1898333.5
56	1633780.5
57	1373840.0
58	1155458.0
59	987256.0
60	858142.0
61	757748.5
62	675567.5
63	595830.0
64	535543.5
65	482850.0
66	426489.0
67	371393.5
68	302003.5
69	232281.5
70	185723.5
71	145230.0
72	110180.0
73	86084.5
74	72676.5
75	55943.0
76	35753.0
77	29625.5
78	21539.5
79	13696.0
80	12512.0
81	8914.5
82	3691.0
83	598.0
84	384.0
85	297.5
86	212.0
87	159.5
88	139.0
89	124.5
90	95.5
91	63.0
92	58.0
93	63.5
94	65.0
95	90.5
96	188.0
97	508.0
98	1535.0
99	1797.0
100	679.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08504974601737351
2	0.05864975848353097
3	0.050873741881909754
4	0.04897538471908342
5	0.04900883594662222
6	0.04897538471908342
7	0.04897538471908342
8	0.04897538471908342
9	0.04897817232137833
10-11	0.04897538471908342
12-13	0.04899768553744262
14-15	0.04904855927932452
16-17	0.04898165682424695
18-19	0.04917121378030008
20-21	0.04901092664834338
22-23	0.049617927048057675
24-25	0.05215464513641592
26-27	0.054375667264876824
28-29	0.05691656675667742
30-31	0.05893479081818441
32-33	0.06098228470378785
34-35	0.06307298642496222
36-37	1.1715539830945597E-4
38-39	3.2776244341573686E-5
40-41	2.9987395948792074E-5
42-43	3.542767818880765E-4
44-45	7.671522319523549E-5
46-47	1.8342313488242703E-4
48-49	1.6878090438513643E-4
50-51	5.92838452902549E-5
52-53	1.0950331493387028E-4
54-55	3.8361955921010616E-5
56-57	6.975080353623183E-7
58-59	0.0
60-61	0.0
62-63	4.882954090681884E-6
64-65	3.4879222078385735E-6
66-67	8.371253542269777E-6
68-69	0.0
70-71	2.092941110309026E-6
72-73	1.1162732158230193E-5
74-75	0.0
76-77	2.093164709937921E-6
78-79	0.0
80-81	6.977785855882932E-7
82-83	6.001149890099755E-5
84-85	6.978385664642875E-6
86-87	6.978729732032899E-6
88-89	2.707891532920088E-4
90-91	4.676369216128705E-5
92-93	8.377075162078255E-6
94-95	5.836787190480708E-4
96-97	2.9356320935574206E-5
98-99	2.4894821158805557E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	46245.0
36-37	1374.0
38-39	1506.0
40-41	1390.0
42-43	1608.0
44-45	1606.0
46-47	1570.0
48-49	1521.0
50-51	1681.0
52-53	1662.0
54-55	1875.0
56-57	1827.0
58-59	1897.0
60-61	2065.0
62-63	2000.0
64-65	2038.0
66-67	2112.0
68-69	2193.0
70-71	2410.0
72-73	2553.0
74-75	2628.0
76-77	2764.0
78-79	2992.0
80-81	3078.0
82-83	3009.0
84-85	3441.0
86-87	3691.0
88-89	4634.0
90-91	12258.0
92-93	8545.0
94-95	27549.0
96-97	505261.0
98-99	6492955.0
100-101	6.4592308E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.5602970057132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.26176633722846	16.354130536629945
2	17.038855680864106	9.732695577659248
3	8.328594323507724	7.136013825584328
4	4.58990174717231	5.243558285051323
5	2.8365426450730773	4.05062502063292
6	1.8550451233911838	3.1788383809831253
7	1.359922694018691	2.718785723218841
8	0.9980616607572514	2.2803949968993975
9	0.7563982834531577	1.944266366703044
>10	4.430620617475482	23.144331981100255
>50	0.3257255189353573	6.414579077301637
>100	0.19534621441380348	10.631711023477763
>500	0.01540700363362808	3.0009564323920475
>1k	0.007587512183705782	3.6469433367799597
>5k	1.7971031378567577E-4	0.3260808230352621
>10k+	4.492757844641894E-5	0.19608861255073004
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3938011474495821E-6	1.3938011474495821E-6	1.254421032704624E-5	0.0	1.9513216064294153E-5
2	2.7876022948991643E-6	1.3938011474495821E-6	1.6725613769394988E-5	1.3938011474495821E-6	3.066362524389081E-5
3	5.575204589798329E-6	1.3938011474495821E-6	1.811941491684457E-5	2.7876022948991643E-6	4.5995437865836216E-5
4	1.1150409179596657E-5	1.3938011474495821E-6	2.6482221801542063E-5	2.7876022948991643E-6	4.878304016073538E-5
5	1.6725613769394988E-5	5.575204589798329E-6	3.484502868623956E-5	4.181403442348747E-6	5.017684130818496E-5
6	2.7876022948991644E-5	5.575204589798329E-6	5.017684130818496E-5	4.181403442348747E-6	5.1570642455634545E-5
7	2.7876022948991644E-5	5.575204589798329E-6	6.27210516352312E-5	4.181403442348747E-6	5.1570642455634545E-5
8	3.763263098113872E-5	5.575204589798329E-6	6.690245507757995E-5	4.181403442348747E-6	5.7145847045432875E-5
9	3.763263098113872E-5	5.575204589798329E-6	7.108385851992869E-5	5.575204589798329E-6	6.132725048778162E-5
10-11	4.390473614466184E-5	5.575204589798329E-6	9.129397515794763E-5	5.575204589798329E-6	6.96900573724791E-5
12-13	5.226754302935933E-5	9.756608032147076E-6	1.1498859466459052E-4	5.575204589798329E-6	8.711257171559888E-5
14-15	6.829625622502953E-5	1.0453508605871867E-5	1.338049101551599E-4	5.575204589798329E-6	1.0244438433754429E-4
16-17	8.711257171559888E-5	1.1150409179596657E-5	1.5819643023552758E-4	5.575204589798329E-6	1.0732268835361783E-4
18-19	9.965678204264513E-5	1.1150409179596657E-5	1.721344417100234E-4	8.362806884697494E-6	1.1150409179596657E-4
20-21	1.1080719122224179E-4	1.1150409179596657E-5	1.9164765777431757E-4	9.756608032147076E-6	1.1150409179596657E-4
22-23	1.3032040728653594E-4	1.184730975332145E-5	2.2370508416565794E-4	1.324111090077103E-5	1.1707929638576491E-4
24-25	1.5959023138297716E-4	1.324111090077103E-5	2.271895870342819E-4	1.811941491684457E-5	1.1916999810693927E-4
26-27	1.7980034802099612E-4	1.6725613769394988E-5	2.3415859277152982E-4	2.0907017211743734E-5	1.2683590441791198E-4
28-29	2.1116087383861172E-4	1.811941491684457E-5	2.4252139965622732E-4	2.0907017211743734E-5	1.3519871130260948E-4
30-31	2.348554933452546E-4	2.0210116638018943E-5	2.529749082620992E-4	2.0907017211743734E-5	1.414708164661326E-4
32-33	2.690036214577694E-4	2.36946195066429E-5	2.66912919736595E-4	2.0907017211743734E-5	1.4495531933475655E-4
34-35	3.0803005358635767E-4	2.9269824096441228E-5	2.989703461279354E-4	2.0907017211743734E-5	1.498336233508301E-4
36-37	3.449657839937716E-4	3.066362524389081E-5	3.129083576024312E-4	2.1603917785468524E-5	1.73528242857473E-4
38-39	3.860829178435343E-4	3.13605258176156E-5	3.289370707981014E-4	2.2300818359193314E-5	1.895569560531432E-4
40-41	4.1953414538232423E-4	3.275432696506518E-5	3.63782099484341E-4	2.2300818359193314E-5	1.9652596179039108E-4
42-43	4.6692338439561E-4	3.4148128112514763E-5	3.9723332702313094E-4	2.2300818359193314E-5	2.0558566924881337E-4
44-45	4.975870096395009E-4	3.90264321285883E-5	4.0768683562900283E-4	2.2997718932918105E-5	2.3067408990290586E-4
46-47	5.540359561112089E-4	4.042023327603789E-5	4.4253186431524234E-4	2.508842065409248E-5	2.4530900195112647E-4
48-49	6.132725048778162E-4	4.042023327603789E-5	4.529853729211142E-4	2.508842065409248E-5	2.6203461572052146E-4
50-51	6.634493461860011E-4	4.181403442348747E-5	4.711047878379588E-4	2.5785321227817273E-5	2.9339514153813706E-4
52-53	7.226858949526084E-4	4.320783557093705E-5	4.975870096395009E-4	2.8572923522716434E-5	3.0175794842283456E-4
54-55	7.763472391294174E-4	4.460163671838663E-5	5.205847285724189E-4	3.4148128112514763E-5	3.108176558812569E-4
56-57	8.327961856011254E-4	5.087374188190975E-5	5.477638509476857E-4	3.623882983368914E-5	3.1848356219222956E-4
58-59	9.122428510057515E-4	5.366134417680892E-5	5.623987629959065E-4	3.763263098113872E-5	3.254525679294775E-4
60-61	9.714793997723588E-4	5.7145847045432875E-5	5.97243791682146E-4	4.1117133849762674E-5	3.30330871945551E-4
62-63	0.0010397756559973883	5.993344934033204E-5	6.439361301217069E-4	4.3904736144661835E-5	3.30330871945551E-4
64-65	0.0010920431990267478	6.341795220895599E-5	6.669338490546251E-4	4.73892390132858E-5	3.4287508227259724E-4
66-67	0.0011540673500882541	6.411485278268078E-5	6.725090536444234E-4	5.017684130818496E-5	3.540254914521939E-4
68-69	0.0012098193959862373	6.411485278268078E-5	6.857501645451945E-4	5.226754302935933E-5	3.5960069604199223E-4
70-71	0.0012592993367206975	6.829625622502953E-5	6.864470651189192E-4	5.2964443603084126E-5	3.853860172698095E-4
72-73	0.0013324738969618007	6.899315679875431E-5	7.066571817569381E-4	5.923654876660724E-5	3.895674207121582E-4
74-75	0.0014216771703985738	6.969005737247911E-5	7.094447840518374E-4	6.690245507757995E-5	4.0002092931803014E-4
76-77	0.0014823075203126306	7.108385851992869E-5	7.171106903628101E-4	6.829625622502953E-5	4.2023104595604905E-4
78-79	0.001540847168505513	7.247765966737828E-5	7.198982926577092E-4	6.829625622502953E-5	4.467132677575911E-4
80-81	0.0015896302086662486	7.526526196227744E-5	7.296549006898563E-4	6.829625622502953E-5	4.6483268267443563E-4
82-83	0.0016195969333364147	7.80528642571766E-5	7.338363041322051E-4	7.178075909365349E-5	4.766799924277571E-4
84-85	0.0016711675757920491	7.944666540462619E-5	7.345332047059298E-4	7.805286425717661E-5	4.857396998861794E-4
86-87	0.001698346698167316	8.084046655207577E-5	7.421991110169025E-4	8.223426769952535E-5	4.94102506770877E-4
88-89	0.0017032250021833896	8.153736712580056E-5	7.484712161804257E-4	8.502186999442452E-5	5.129188222614462E-4
90	0.0017032250021833896	8.362806884697493E-5	7.526526196227745E-4	8.502186999442452E-5	5.17100225703795E-4
>>END_MODULE
