##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290289_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72507403
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.28148074755898	37.0	37.0	37.0	37.0	37.0
2	36.29894041302238	37.0	37.0	37.0	37.0	37.0
3	36.38181821241067	37.0	37.0	37.0	37.0	37.0
4	36.40411699754299	37.0	37.0	37.0	37.0	37.0
5	36.417235478700015	37.0	37.0	37.0	37.0	37.0
6	36.39129125890773	37.0	37.0	37.0	37.0	37.0
7	36.343217767708495	37.0	37.0	37.0	37.0	37.0
8	36.40191174685983	37.0	37.0	37.0	37.0	37.0
9	36.38178191266897	37.0	37.0	37.0	37.0	37.0
10-11	36.40175565934971	37.0	37.0	37.0	37.0	37.0
12-13	36.37865109442687	37.0	37.0	37.0	37.0	37.0
14-15	36.36921960920321	37.0	37.0	37.0	37.0	37.0
16-17	36.36739019600523	37.0	37.0	37.0	37.0	37.0
18-19	36.36100713054086	37.0	37.0	37.0	37.0	37.0
20-21	36.35487012271009	37.0	37.0	37.0	37.0	37.0
22-23	36.339474970576454	37.0	37.0	37.0	37.0	37.0
24-25	36.30930199637684	37.0	37.0	37.0	37.0	37.0
26-27	36.2644247650133	37.0	37.0	37.0	37.0	37.0
28-29	36.25025326724224	37.0	37.0	37.0	37.0	37.0
30-31	36.2407483136584	37.0	37.0	37.0	37.0	37.0
32-33	36.233588424067534	37.0	37.0	37.0	37.0	37.0
34-35	36.22030809047181	37.0	37.0	37.0	37.0	37.0
36-37	36.21069142605403	37.0	37.0	37.0	37.0	37.0
38-39	36.20203621286897	37.0	37.0	37.0	37.0	37.0
40-41	36.19795064543561	37.0	37.0	37.0	37.0	37.0
42-43	36.18202423260469	37.0	37.0	37.0	37.0	37.0
44-45	36.182849657995995	37.0	37.0	37.0	37.0	37.0
46-47	36.177489676173664	37.0	37.0	37.0	37.0	37.0
48-49	36.15049296930043	37.0	37.0	37.0	37.0	37.0
50-51	36.144676951384724	37.0	37.0	37.0	37.0	37.0
52-53	36.14283755964057	37.0	37.0	37.0	37.0	37.0
54-55	36.13323294681298	37.0	37.0	37.0	37.0	37.0
56-57	36.11922552591359	37.0	37.0	37.0	37.0	37.0
58-59	36.10866180844032	37.0	37.0	37.0	37.0	37.0
60-61	36.106400623964426	37.0	37.0	37.0	37.0	37.0
62-63	36.09422578752305	37.0	37.0	37.0	37.0	37.0
64-65	36.07831944493501	37.0	37.0	37.0	37.0	37.0
66-67	36.07322280757505	37.0	37.0	37.0	37.0	37.0
68-69	36.058007820666944	37.0	37.0	37.0	37.0	37.0
70-71	36.03652622260306	37.0	37.0	37.0	37.0	37.0
72-73	36.01499189712627	37.0	37.0	37.0	37.0	37.0
74-75	36.008703457443886	37.0	37.0	37.0	37.0	37.0
76-77	35.99690391430261	37.0	37.0	37.0	37.0	37.0
78-79	35.98428749455593	37.0	37.0	37.0	37.0	37.0
80-81	35.95651007887603	37.0	37.0	37.0	37.0	37.0
82-83	35.9684442580357	37.0	37.0	37.0	37.0	37.0
84-85	35.95204675764842	37.0	37.0	37.0	37.0	37.0
86-87	35.963729872201114	37.0	37.0	37.0	37.0	37.0
88-89	35.93889175294763	37.0	37.0	37.0	37.0	37.0
90-91	35.923172938539544	37.0	37.0	37.0	37.0	37.0
92-93	35.90930699118724	37.0	37.0	37.0	37.0	37.0
94-95	35.88175495919117	37.0	37.0	37.0	37.0	37.0
96-97	35.880164229300576	37.0	37.0	37.0	37.0	37.0
98-99	35.858837145765094	37.0	37.0	37.0	37.0	37.0
100-101	35.81178751577864	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9952.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	13.0
15	297.0
16	1300.0
17	3732.0
18	6686.0
19	9539.0
20	12067.0
21	15435.0
22	21102.0
23	34312.0
24	57929.0
25	98298.0
26	153348.0
27	239068.0
28	350284.0
29	503090.0
30	669521.0
31	888094.0
32	1131292.0
33	1557238.0
34	2367795.0
35	5416854.0
36	4.240094E7
37	1.6559215E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.133877647081423	15.4722869086796	14.970229173764912	49.42360627047407
2	21.87632429489658	20.06280617815268	33.66434655983269	24.396522967118052
3	23.76684268583173	21.72054454307552	23.335302129357803	31.177310641734945
4	24.235779327410825	26.68324610038008	20.54713648549018	28.53383808671891
5	26.148502452411105	30.819228086005644	22.888275113866452	20.143994347716802
6	20.703409282624296	35.00994124607222	22.64729693737784	21.639352533925642
7	18.635434506518028	20.65168884351534	39.91648892593479	20.79638772403184
8	19.861181050351686	23.683838208325422	29.488821613879914	26.966159127442978
9	20.77478811081444	22.13227591310905	31.639940369210517	25.45299560686599
10-11	23.061386137985636	30.953963684557433	23.158014363553377	22.826635813903554
12-13	21.973517339837013	25.815287609303432	27.37668279654712	24.83451225431244
14-15	22.229062315208864	26.44966115331876	26.79617224840784	24.525104283064536
16-17	23.073821429155192	26.58151952803302	26.078191445229553	24.26646759758223
18-19	22.839547523885628	26.9370948707302	25.805032138607352	24.418325466776817
20-21	23.048057595865544	26.85293613044965	26.049891067856674	24.049115205828137
22-23	22.766132413086964	26.932850317327546	26.30437201740852	23.99664525217697
24-25	22.543243805675463	26.95587941658943	26.42974104784631	24.0711357298888
26-27	22.750678582066154	26.99570965999851	26.349199171983457	23.904412585951878
28-29	22.71789752942327	26.889317617474518	26.231730348609904	24.16105450449231
30-31	22.67600921803527	26.883847295829682	26.258817405413826	24.18132608072122
32-33	22.940336383784583	26.93911646261928	26.15725288143887	23.963294272157274
34-35	22.948051161493595	26.818357708162438	26.063423101008638	24.170168029335333
36-37	22.747638135581763	26.78355905647369	26.22015062188015	24.2486521860644
38-39	23.090033902743766	26.929943172074754	26.123869617123972	23.8561533080575
40-41	23.09300626991387	26.786909726947034	26.061498802201783	24.058585200937312
42-43	22.889284597778158	26.690978669870873	26.29735057119116	24.122386161159813
44-45	23.01830141760213	26.737117032936187	26.319972476940755	23.924609072520926
46-47	22.964656888852414	26.725374470182846	26.195853402688922	24.114115238275822
48-49	22.834376138979636	26.66414137592403	26.219521389574503	24.281961095521837
50-51	23.15963984224101	26.744468962879047	26.08006104941218	24.015830145467767
52-53	23.298102878342267	26.75138846054064	25.940838488567604	24.009670172549495
54-55	22.888022839015218	26.680063310978415	26.261290040239736	24.17062380976663
56-57	23.072798929120573	26.809351022862206	26.22060160891847	23.897248439098753
58-59	23.146623149016264	26.643732417140708	26.182007686786662	24.02763674705637
60-61	23.041841132633227	26.592307572906627	26.185528882388752	24.180322412071394
62-63	23.150901862131708	26.63478148153583	26.263286905203902	23.951029751128562
64-65	23.192166867526055	26.70169377295612	26.116895195444034	23.989244164073792
66-67	23.122797830396095	26.66018913803157	26.195293728252985	24.02171930331935
68-69	23.323186503741518	26.736039979428522	26.11330637110047	23.827467145729493
70-71	23.399001607602496	26.7149920741776	26.007649459444576	23.87835685877533
72-73	23.188514218638236	26.694122707834083	26.137320695611006	23.980042377916675
74-75	23.240034123711787	26.699946863528034	26.258254598143115	23.801764414617065
76-77	23.278792595309366	26.631592816807498	26.15346110255828	23.936153485324862
78-79	23.091746908821154	26.63876101507413	26.257206224126246	24.012285851978472
80-81	23.373595428491537	26.723708553732	26.14544577537411	23.757250242402357
82-83	23.378567766948898	26.72594990998996	26.024828384495493	23.87065393856565
84-85	23.240543452233904	26.701369620852404	26.117571546880587	23.940515380033105
86-87	23.353958021851636	26.6859643723371	26.174922093133258	23.785155512678006
88-89	23.476078110455294	26.501998345003443	26.135019525762626	23.88690401877864
90-91	23.248172540922564	26.565029206627983	26.26939995897733	23.917398293472118
92-93	23.39649211449595	26.627759983105005	26.2051679687705	23.770579933628554
94-95	23.479271865992533	26.579840510810282	26.13261038262597	23.80827724057121
96-97	23.367696349462634	26.455329456442694	26.237521671445798	23.939452522648878
98-99	23.560004517497838	25.60851717706291	26.710310031495744	24.121168273943507
100-101	24.276802260790028	12.071013957367674	33.088853215198014	30.563330566644286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9981.0
1	4998.5
2	18.5
3	18.0
4	23.5
5	36.0
6	45.5
7	61.5
8	78.5
9	94.0
10	127.0
11	194.5
12	283.5
13	421.5
14	679.5
15	1047.0
16	1509.5
17	2166.0
18	2938.0
19	3907.0
20	5297.5
21	7124.5
22	9619.0
23	13214.0
24	18572.5
25	25544.0
26	35416.5
27	50699.5
28	73174.0
29	100941.0
30	140696.0
31	198810.0
32	283812.0
33	393035.0
34	532887.0
35	718377.0
36	956709.0
37	1250968.0
38	1610896.0
39	2032210.5
40	2464578.5
41	2892042.5
42	3331031.0
43	3717741.0
44	4011214.5
45	4217069.0
46	4327188.5
47	4340656.5
48	4194276.5
49	3920186.5
50	3597672.0
51	3260058.5
52	2940265.0
53	2603592.0
54	2262398.5
55	1968582.0
56	1695219.0
57	1404334.0
58	1123156.0
59	904441.0
60	763219.5
61	661297.5
62	578621.0
63	510079.0
64	446979.0
65	397233.0
66	353610.5
67	308436.5
68	250203.5
69	185909.0
70	138733.5
71	105595.5
72	76616.0
73	55622.5
74	42819.0
75	31673.5
76	19769.0
77	11170.0
78	6519.0
79	3743.0
80	2681.5
81	2107.5
82	1497.0
83	1063.0
84	861.5
85	732.0
86	636.0
87	542.5
88	461.5
89	390.0
90	323.0
91	322.5
92	347.5
93	372.5
94	447.0
95	702.5
96	1475.5
97	3994.5
98	12994.0
99	15410.5
100	5659.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050277624755088804
2	0.02531603566052421
3	0.01561219893643136
4	0.013726874206210365
5	0.013755836766074768
6	0.013725495036693012
7	0.013725495036693012
8	0.013725495036693012
9	0.013731011714762422
10-11	0.013726184621451688
12-13	0.01374411382517727
14-15	0.013809624377251521
16-17	0.013733770053797126
18-19	0.01392340586243311
20-21	0.013759974274626826
22-23	0.013999260185887501
24-25	0.015142591715772803
26-27	0.016205241828892977
28-29	0.01750235075996309
30-31	0.018522246618045333
32-33	0.019575932129302715
34-35	0.020730986600085512
36-37	1.2760107462176368E-4
38-39	2.7589779106985893E-5
40-41	2.8969635724626394E-5
42-43	3.4626074845612713E-4
44-45	8.415227464460581E-5
46-47	1.724457360001602E-4
48-49	1.4761575040742639E-4
50-51	6.415183871068877E-5
52-53	1.0071332975065207E-4
54-55	3.449150804585478E-5
56-57	6.898431665705313E-7
58-59	1.3797139539778335E-6
60-61	0.0
62-63	3.449448484239208E-6
64-65	2.7596260309341935E-6
66-67	2.759702816710688E-6
68-69	0.0
70-71	5.5198070617679245E-6
72-73	4.830068229060796E-6
74-75	0.0
76-77	1.3801882302088548E-6
78-79	0.0
80-81	0.0
82-83	6.350884490444238E-5
84-85	8.2851615056566E-6
86-87	9.668026822227796E-6
88-89	2.4590449177295436E-4
90-91	4.97495500550503E-5
92-93	3.4565364597677956E-6
94-95	6.06582148078502E-4
96-97	3.3944562682361966E-5
98-99	2.258183862967306E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	15624.0
36-37	918.0
38-39	953.0
40-41	924.0
42-43	1079.0
44-45	1069.0
46-47	1086.0
48-49	1117.0
50-51	1346.0
52-53	1331.0
54-55	1378.0
56-57	1395.0
58-59	1632.0
60-61	1742.0
62-63	1759.0
64-65	1943.0
66-67	2199.0
68-69	2779.0
70-71	3426.0
72-73	3924.0
74-75	4572.0
76-77	5519.0
78-79	7014.0
80-81	9057.0
82-83	11504.0
84-85	14436.0
86-87	16957.0
88-89	21246.0
90-91	33986.0
92-93	34462.0
94-95	59055.0
96-97	560297.0
98-99	6714135.0
100-101	6.4967539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.581532647906837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.01140896301513	14.357060307098346
2	17.597075205032358	9.35514458140479
3	8.892624985664732	7.091388041461173
4	5.125248214015095	5.449478109178738
5	3.191291941451499	4.241471546534751
6	2.1094448264582786	3.3643365914075343
7	1.5640179855395095	2.9101796601172367
8	1.1063740747019943	2.3527294869991024
9	0.8755430644883603	2.094594889801118
>10	4.935748485642716	24.00186803976527
>50	0.3561518253282234	6.551214865156631
>100	0.20851005398275194	10.54268837771792
>500	0.017755483519920325	3.22789423032257
>1k	0.00851812531011245	3.905387013058962
>5k	2.5015744299261404E-4	0.43364267249029015
>10k+	3.660840629160206E-5	0.12092158748552993
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.516678069410375E-6
2	0.0	0.0	0.0	0.0	8.275017104115561E-6
3	6.895847586762968E-6	0.0	0.0	0.0	1.6550034208231123E-5
4	3.447923793381484E-5	0.0	1.7929203725583716E-5	0.0	2.0687542760288906E-5
5	3.9995916003225215E-5	0.0	3.1720898899109655E-5	4.137508552057781E-6	2.0687542760288906E-5
6	4.4133424555283E-5	0.0	6.068345876351412E-5	4.137508552057781E-6	2.0687542760288906E-5
7	5.5166780694103744E-5	6.895847586762968E-6	8.275017104115562E-5	4.137508552057781E-6	2.0687542760288906E-5
8	6.206262828086671E-5	8.275017104115561E-6	8.412934055850821E-5	5.516678069410375E-6	2.20667122776415E-5
9	6.206262828086671E-5	9.654186621468156E-6	8.688767959321339E-5	8.275017104115561E-6	2.620422082969928E-5
10-11	7.516473869571635E-5	1.103335613882075E-5	1.0274812904276822E-4	8.275017104115561E-6	2.6893805588375577E-5
12-13	8.481892531718451E-5	1.2412525656173343E-5	1.4205446028731715E-4	8.964601862791858E-6	3.585840745116743E-5
14-15	9.930020524938674E-5	1.310211041484964E-5	1.6963785063436903E-4	9.654186621468156E-6	3.9995916003225215E-5
16-17	1.055064680774734E-4	1.4481279932202233E-5	2.2066712277641498E-4	9.654186621468156E-6	4.137508552057781E-5
18-19	1.1791899373364675E-4	1.517086469087853E-5	2.3514840270861723E-4	1.1722940897497046E-5	4.2064670279254107E-5
20-21	1.268835955964386E-4	1.517086469087853E-5	2.606630387796402E-4	1.2412525656173343E-5	4.6891763589988185E-5
22-23	1.310211041484964E-4	1.5860449449554824E-5	2.8824642912669206E-4	1.2412525656173343E-5	5.102927214204597E-5
24-25	1.5791490973687198E-4	1.6550034208231123E-5	2.951422767134551E-4	1.6550034208231123E-5	5.102927214204597E-5
26-27	1.7584411346245566E-4	1.7929203725583716E-5	3.0686521761095207E-4	2.20667122776415E-5	5.7235534970132636E-5
28-29	2.0480667332686017E-4	1.7929203725583716E-5	3.289319298885936E-4	2.413546655367039E-5	5.9993874004837816E-5
30-31	2.344588179499409E-4	1.9997958001612607E-5	3.3237985368197504E-4	2.4825051312346686E-5	6.41313825568956E-5
32-33	2.544567759515535E-4	2.4825051312346686E-5	3.4272362506211954E-4	2.4825051312346686E-5	6.757930635027708E-5
34-35	2.71696394918461E-4	2.8962559864404465E-5	4.247842113445988E-4	2.4825051312346686E-5	7.033764538498227E-5
36-37	2.944526919547787E-4	2.8962559864404465E-5	4.365071522420959E-4	2.4825051312346686E-5	7.930224724777413E-5
38-39	3.2548400609521213E-4	3.1031314140433354E-5	4.509884321742981E-4	2.4825051312346686E-5	9.585228145600526E-5
40-41	3.4755071837285357E-4	3.3100068416462245E-5	4.730551444519396E-4	2.4825051312346686E-5	1.0343771380144453E-4
42-43	3.8065078678931584E-4	3.9995916003225215E-5	4.965010262469337E-4	2.620422082969928E-5	1.2757318035511492E-4
44-45	4.116821009297492E-4	3.9995916003225215E-5	5.013281195576677E-4	2.620422082969928E-5	1.455023840806986E-4
46-47	4.316800589313618E-4	3.9995916003225215E-5	5.330490184567774E-4	2.896255986440447E-5	1.5101906215010902E-4
48-49	4.751238987279685E-4	4.068550076190152E-5	5.613219935625056E-4	3.447923793381484E-5	1.6205241828892974E-4
50-51	5.061552128684018E-4	4.137508552057781E-5	5.826991210814708E-4	3.585840745116743E-5	1.703274353930453E-4
52-53	5.3442818797413E-4	4.137508552057781E-5	6.199366980499908E-4	4.4133424555283E-5	1.758441134624557E-4
54-55	5.640803325972108E-4	4.27542550379304E-5	6.544159359838056E-4	4.4133424555283E-5	1.8136079153186608E-4
56-57	5.820095363227945E-4	4.27542550379304E-5	6.92343097711002E-4	4.4133424555283E-5	1.8963580863598162E-4
58-59	6.233846218433723E-4	4.27542550379304E-5	7.109618861952621E-4	4.4133424555283E-5	1.9722124098142088E-4
60-61	6.792409872961524E-4	4.4133424555283E-5	7.87505794408331E-4	4.965010262469337E-5	2.0273791905083127E-4
62-63	7.261327508861406E-4	4.551259407263559E-5	8.585330245519895E-4	4.965010262469337E-5	2.0411708856818387E-4
64-65	7.654390821306895E-4	4.551259407263559E-5	8.888747539337466E-4	4.965010262469337E-5	2.1101293615494683E-4
66-67	8.240537866181747E-4	4.551259407263559E-5	8.94391432003157E-4	5.171885690072226E-5	2.248046313284728E-4
68-69	8.87495584416394E-4	4.965010262469337E-5	9.033560338659488E-4	5.3787611176751154E-5	2.3101089415655942E-4
70-71	9.426623651104978E-4	4.965010262469337E-5	9.088727119353592E-4	5.3787611176751154E-5	2.385963265019987E-4
72-73	0.0010123104257368037	5.102927214204596E-5	9.350769327650585E-4	5.5166780694103744E-5	2.5445677595155357E-4
74-75	0.0010743730540176705	5.240844165939856E-5	9.419727803518215E-4	6.206262828086672E-5	2.620422082969928E-4
76-77	0.0011164377242969246	5.5166780694103744E-5	9.516269669732895E-4	6.48209673155719E-5	2.654901320903743E-4
78-79	0.001159191979334855	5.7235534970132636E-5	9.571436450427E-4	6.48209673155719E-5	2.7100681015978465E-4
80-81	0.0012005670648554327	5.792511972880893E-5	9.585228145600526E-4	6.48209673155719E-5	2.847985053333106E-4
82-83	0.0012350463027892477	5.792511972880893E-5	9.736936792509311E-4	6.48209673155719E-5	2.9307352243742614E-4
84-85	0.001294350592035409	5.792511972880893E-5	9.77831187802989E-4	6.48209673155719E-5	2.972110309894839E-4
86-87	0.0013343465080386343	5.792511972880893E-5	9.930020524938675E-4	6.620013683292449E-5	3.1031314140433355E-4
88-89	0.001347448618453484	6.068345876351412E-5	0.0010040354086326881	6.964806062630599E-5	3.247944213365358E-4
90	0.001347448618453484	6.206262828086671E-5	0.001008172917184746	7.171681490233486E-5	3.268631756125647E-4
>>END_MODULE
