##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290301_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88528711
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.094291974950366	37.0	37.0	37.0	37.0	37.0
2	36.207690881210276	37.0	37.0	37.0	37.0	37.0
3	36.324304292649195	37.0	37.0	37.0	37.0	37.0
4	36.35472917932805	37.0	37.0	37.0	37.0	37.0
5	36.37081138569837	37.0	37.0	37.0	37.0	37.0
6	36.33638867734107	37.0	37.0	37.0	37.0	37.0
7	36.29986599488611	37.0	37.0	37.0	37.0	37.0
8	36.35941243965475	37.0	37.0	37.0	37.0	37.0
9	36.34360530788707	37.0	37.0	37.0	37.0	37.0
10-11	36.364873131384456	37.0	37.0	37.0	37.0	37.0
12-13	36.34548244467267	37.0	37.0	37.0	37.0	37.0
14-15	36.340190703781964	37.0	37.0	37.0	37.0	37.0
16-17	36.3394120637315	37.0	37.0	37.0	37.0	37.0
18-19	36.33470087461231	37.0	37.0	37.0	37.0	37.0
20-21	36.329109163240844	37.0	37.0	37.0	37.0	37.0
22-23	36.315442286288345	37.0	37.0	37.0	37.0	37.0
24-25	36.28545515589852	37.0	37.0	37.0	37.0	37.0
26-27	36.24190588294005	37.0	37.0	37.0	37.0	37.0
28-29	36.22849423392147	37.0	37.0	37.0	37.0	37.0
30-31	36.21795631927816	37.0	37.0	37.0	37.0	37.0
32-33	36.2121735738364	37.0	37.0	37.0	37.0	37.0
34-35	36.199221860352175	37.0	37.0	37.0	37.0	37.0
36-37	36.194486708299706	37.0	37.0	37.0	37.0	37.0
38-39	36.18608288952127	37.0	37.0	37.0	37.0	37.0
40-41	36.18395227068268	37.0	37.0	37.0	37.0	37.0
42-43	36.167697106334145	37.0	37.0	37.0	37.0	37.0
44-45	36.16722748402639	37.0	37.0	37.0	37.0	37.0
46-47	36.16325297576122	37.0	37.0	37.0	37.0	37.0
48-49	36.13619366659816	37.0	37.0	37.0	37.0	37.0
50-51	36.12883260791068	37.0	37.0	37.0	37.0	37.0
52-53	36.1277720683138	37.0	37.0	37.0	37.0	37.0
54-55	36.11774590235214	37.0	37.0	37.0	37.0	37.0
56-57	36.10349244590519	37.0	37.0	37.0	37.0	37.0
58-59	36.092990592454576	37.0	37.0	37.0	37.0	37.0
60-61	36.08868823352422	37.0	37.0	37.0	37.0	37.0
62-63	36.077783130912906	37.0	37.0	37.0	37.0	37.0
64-65	36.06077187361057	37.0	37.0	37.0	37.0	37.0
66-67	36.056997790594096	37.0	37.0	37.0	37.0	37.0
68-69	36.0417576207219	37.0	37.0	37.0	37.0	37.0
70-71	36.019956776817416	37.0	37.0	37.0	37.0	37.0
72-73	35.99527206528082	37.0	37.0	37.0	37.0	37.0
74-75	35.99136898047311	37.0	37.0	37.0	37.0	37.0
76-77	35.97891622403641	37.0	37.0	37.0	37.0	37.0
78-79	35.9661288022682	37.0	37.0	37.0	37.0	37.0
80-81	35.937287743992485	37.0	37.0	37.0	37.0	37.0
82-83	35.949162319783895	37.0	37.0	37.0	37.0	37.0
84-85	35.93121321118058	37.0	37.0	37.0	37.0	37.0
86-87	35.94485794302035	37.0	37.0	37.0	37.0	37.0
88-89	35.91980703201554	37.0	37.0	37.0	37.0	37.0
90-91	35.90315555536795	37.0	37.0	37.0	37.0	37.0
92-93	35.89022534410249	37.0	37.0	37.0	37.0	37.0
94-95	35.86027613638025	37.0	37.0	37.0	37.0	37.0
96-97	35.85913216675456	37.0	37.0	37.0	37.0	37.0
98-99	35.83663122840876	37.0	37.0	37.0	37.0	37.0
100-101	35.809565771803804	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26165.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	16.0
15	197.0
16	1065.0
17	2813.0
18	4474.0
19	6430.0
20	8710.0
21	13005.0
22	20282.0
23	35766.0
24	63181.0
25	108246.0
26	169929.0
27	267808.0
28	400279.0
29	586567.0
30	798363.0
31	1088969.0
32	1434733.0
33	2046587.0
34	3227244.0
35	7497180.0
36	5.2492158E7
37	1.8228544E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.25151259653799	15.859978466652638	12.50655969311979	46.38194924368958
2	21.888668329932965	19.264043875118023	32.57883818744971	26.2684496074993
3	23.920091425920546	20.05913699832864	23.213109112591244	32.80766246315957
4	24.99866642253056	25.290731469925525	20.591506153862582	29.119095953681335
5	26.14038345035901	29.226809968964496	22.84292789339132	21.789878687285167
6	21.64184406627127	33.69129742324023	22.740642964101845	21.926215546386654
7	18.88856282168425	22.135635510417973	38.10699185987259	20.868809808025183
8	19.870687109950484	24.169761172746377	29.512476398136616	26.447075319166522
9	20.821685975245117	22.43332776475524	31.499376656343614	25.245609603656032
10-11	22.683539778432362	30.86131308427345	23.754393729581448	22.70075340771274
12-13	22.049437897145776	26.009490203220796	27.312445037941597	24.628626861691824
14-15	22.14470548132055	26.58283298895213	26.846969716135405	24.425491813591915
16-17	22.8684425656935	26.508861432563137	26.253374401911913	24.369321599831455
18-19	22.78132778359049	26.825709244654146	25.915709772210704	24.477253199544656
20-21	22.904712157455226	26.66390011637861	26.19388072099824	24.23750700516792
22-23	22.79367099007102	26.751811736486193	26.2507058154128	24.203811458029985
24-25	22.649561113661864	26.71485374308457	26.310741005253202	24.32484413800036
26-27	22.701756624444812	26.874636150747595	26.27232930312571	24.151277921681885
28-29	22.586345854567103	26.78653669606021	26.202687740482688	24.42442970889
30-31	22.613745089085157	26.557208449462305	26.301697314364077	24.52734914708846
32-33	22.87120333039875	26.654294399393603	26.17626429847753	24.29823797173012
34-35	22.815921016140535	26.704793096178243	26.027763080626805	24.451522807054417
36-37	22.636783125854343	26.625626098102817	26.221410298602834	24.51618047744001
38-39	22.839841961801746	26.72783906166995	26.219278621596647	24.213040354931653
40-41	22.983970854747582	26.593438150556103	26.0482529325909	24.37433806210542
42-43	22.816765356639287	26.549550041744226	26.284403854010385	24.3492807476061
44-45	22.928572409330794	26.57225247387267	26.28084271268225	24.218332404114285
46-47	22.95802839258438	26.515002361291856	26.183178939666863	24.343790306456903
48-49	22.81767932636417	26.40099702048403	26.263588174797214	24.517735478354584
50-51	22.964352702427114	26.486485081424266	26.158373439370852	24.390788776777768
52-53	23.207176979168292	26.61021300551794	25.899521776280064	24.283088239033695
54-55	22.878773417267077	26.52407119110269	26.1556653100219	24.44149008160834
56-57	22.920272641981697	26.570796401039765	26.195081287340482	24.313849669638056
58-59	23.020803264266483	26.494642203377616	26.14642513747324	24.33812939488266
60-61	22.986889707003208	26.39494434037028	26.147351192834158	24.470814759792354
62-63	23.070240717006655	26.312439148208437	26.21603196655612	24.401288168228792
64-65	23.12165645705381	26.489798384378005	26.004078645527525	24.38446651304066
66-67	23.16342783728435	26.44002739786232	26.04376070815385	24.352784056699477
68-69	23.161451025309944	26.501046349983653	26.08193436128564	24.255568263420763
70-71	23.369958435660397	26.427374796744825	25.95198394845106	24.25068281914372
72-73	23.186106610787334	26.405991614030327	26.026619482341403	24.381282292840943
74-75	23.21374063359707	26.426205544801213	26.07772327373316	24.282330547868558
76-77	23.32474313056632	26.397427108849254	25.934009306796685	24.343820453787743
78-79	23.103685369461967	26.398225731801595	26.02336211981062	24.474726778925813
80-81	23.217405656640643	26.48807992624623	25.99327578218936	24.301238634923767
82-83	23.321182343076206	26.467321335225787	25.857461808370065	24.35403451332795
84-85	23.271090085885618	26.416322692690443	25.93826065803464	24.3743265633893
86-87	23.29072862376564	26.417707338989544	25.950940124738576	24.340623912506242
88-89	23.417454976298895	26.2648262858218	25.906472356468147	24.41124638141116
90-91	23.29968329567062	26.257682638104917	26.0573995179043	24.385234548320163
92-93	23.411320144354324	26.345908242473858	26.021936317528965	24.22083529564285
94-95	23.394008688840533	26.325983568468086	25.999407591656738	24.280600151034648
96-97	23.285817343795305	26.21510946801269	26.02520797376228	24.47386521442972
98-99	23.491730051900014	25.407233605119185	26.508785590377983	24.59225075260282
100-101	24.282189847367313	11.993477347751954	32.718286075298714	31.006046729582014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26666.0
1	13424.5
2	167.0
3	143.5
4	136.5
5	143.5
6	147.0
7	149.5
8	163.5
9	211.0
10	269.5
11	345.5
12	452.5
13	634.0
14	916.5
15	1347.5
16	2032.5
17	2871.0
18	4072.5
19	5644.0
20	7391.0
21	9837.5
22	13375.0
23	18845.5
24	26555.5
25	35695.0
26	48635.5
27	68276.5
28	95367.5
29	130190.5
30	179001.5
31	245998.5
32	340360.0
33	460793.5
34	615465.0
35	827143.5
36	1101362.5
37	1434614.0
38	1850297.5
39	2351536.0
40	2841121.0
41	3331430.5
42	3878887.0
43	4363275.5
44	4765282.5
45	5061451.5
46	5218206.0
47	5263319.0
48	5113838.5
49	4809792.0
50	4463135.0
51	4091523.0
52	3680522.0
53	3228879.0
54	2773744.0
55	2406024.5
56	2091954.0
57	1764758.0
58	1463793.0
59	1213876.5
60	1022259.5
61	886922.5
62	793179.0
63	702484.5
64	613705.0
65	540597.5
66	480540.5
67	420630.0
68	339486.0
69	259316.5
70	205748.5
71	162037.5
72	124468.0
73	96655.5
74	81823.5
75	64228.5
76	40803.5
77	34101.5
78	26307.5
79	18498.5
80	16254.5
81	11009.5
82	4759.0
83	1127.5
84	791.5
85	628.5
86	529.5
87	418.5
88	336.0
89	269.5
90	225.5
91	190.0
92	179.5
93	193.0
94	218.5
95	259.0
96	475.5
97	1181.5
98	3386.0
99	3960.5
100	1558.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06606896151464355
2	0.03994749228868813
3	0.03136835461209867
4	0.02955651302773402
5	0.02959378907030511
6	0.02955538345068641
7	0.02955538345068641
8	0.02955538345068641
9	0.02955651302773402
10-11	0.02955651302773402
12-13	0.02957289189492435
14-15	0.02965309186530458
16-17	0.029567244009686307
18-19	0.029766614358589273
20-21	0.02958701160801946
22-23	0.02989312698792147
24-25	0.031249749022099732
26-27	0.032536337279326255
28-29	0.034042063483788895
30-31	0.03524054473130192
32-33	0.0365994259195754
34-35	0.03803116482741966
36-37	1.2712736055168165E-4
38-39	3.3336136017175544E-5
40-41	3.107655521222115E-5
42-43	3.667088858399754E-4
44-45	8.927727491944591E-5
46-47	1.8816389859866716E-4
48-49	1.4917787058399317E-4
50-51	8.363167709800432E-5
52-53	1.1471352582718809E-4
54-55	4.1817666114914586E-5
56-57	5.65116849154388E-7
58-59	1.1302611189706015E-6
60-61	5.65144385461204E-7
62-63	4.521276428902365E-6
64-65	1.1303509911640915E-6
66-67	7.912706747864753E-6
68-69	0.0
70-71	2.2609343419693036E-6
72-73	1.0174622179254165E-5
74-75	0.0
76-77	4.522428600236441E-6
78-79	0.0
80-81	2.8268028148375238E-6
82-83	5.201627903730329E-5
84-85	1.130863923833872E-5
86-87	4.5237872239619666E-6
88-89	2.804973722853474E-4
90-91	5.0902156451759535E-5
92-93	1.1314079858158549E-5
94-95	5.686300161668586E-4
96-97	3.285531102788911E-5
98-99	2.5940633304245233E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	34480.0
36-37	1337.0
38-39	1425.0
40-41	1301.0
42-43	1404.0
44-45	1652.0
46-47	1720.0
48-49	1660.0
50-51	1932.0
52-53	1959.0
54-55	2058.0
56-57	2116.0
58-59	2078.0
60-61	2405.0
62-63	2435.0
64-65	2731.0
66-67	2945.0
68-69	3184.0
70-71	3655.0
72-73	3540.0
74-75	3693.0
76-77	4031.0
78-79	4588.0
80-81	5225.0
82-83	5563.0
84-85	6473.0
86-87	6924.0
88-89	8001.0
90-91	18672.0
92-93	14419.0
94-95	36080.0
96-97	638749.0
98-99	7985385.0
100-101	7.9714891E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.008857792859258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.20591633626695	4.579887590857974
2	9.494424683296694	2.4702324106003903
3	9.02702002374843	3.52293659346709
4	7.679774322247784	3.9962036815749413
5	6.420701901284185	4.176299898207351
6	5.260545170734474	4.1060210423398384
7	4.084724744675915	3.719632232852362
8	3.2510791362089333	3.383426092501899
9	2.696759450831297	3.157358417767221
>10	15.475612127939293	35.05527597743766
>50	0.8345070153322457	7.460565250453409
>100	0.4924343289466705	12.343267252174021
>500	0.047921249649111035	4.325086538410986
>1k	0.027530097005180984	6.557366216012409
>5k	8.095462711537073E-4	0.6913221209341079
>10k+	2.3986556182332066E-4	0.4551186844081372
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.259154095217765E-6	1.1295770476088824E-5
3	2.259154095217765E-6	0.0	1.2425347523697708E-5	2.259154095217765E-6	3.2757734380657595E-5
4	1.9202809809351002E-5	0.0	2.4850695047395416E-5	2.259154095217765E-6	3.8405619618702005E-5
5	2.259154095217765E-5	0.0	3.049858028543983E-5	2.259154095217765E-6	3.8405619618702005E-5
6	5.3090121237617474E-5	0.0	3.8405619618702005E-5	2.259154095217765E-6	3.8405619618702005E-5
7	5.3090121237617474E-5	0.0	4.970139009479083E-5	2.259154095217765E-6	3.8405619618702005E-5
8	7.003377695175071E-5	0.0	4.970139009479083E-5	2.259154095217765E-6	3.8405619618702005E-5
9	7.681123923740401E-5	0.0	4.970139009479083E-5	2.259154095217765E-6	3.8405619618702005E-5
10-11	8.189433595164398E-5	2.259154095217765E-6	5.817321795185745E-5	1.2425347523697708E-5	4.1794350761528655E-5
12-13	9.601404904675501E-5	4.51830819043553E-6	7.39872966183818E-5	1.4684501618915473E-5	4.4053504856746415E-5
14-15	1.1465207033230157E-4	4.51830819043553E-6	1.0053235723719053E-4	1.4684501618915473E-5	5.196054419000859E-5
16-17	1.2764220637980373E-4	4.51830819043553E-6	1.383731883320881E-4	1.5814078666524356E-5	5.421969828522636E-5
18-19	1.3329009161784815E-4	4.51830819043553E-6	1.4628022766535026E-4	1.5814078666524356E-5	5.421969828522636E-5
20-21	1.4176191947491473E-4	4.51830819043553E-6	1.7056613418894124E-4	1.5814078666524356E-5	6.0432372047075216E-5
22-23	1.4684501618915472E-4	4.51830819043553E-6	2.0840696528383882E-4	1.5814078666524356E-5	7.568166218979513E-5
24-25	1.6265909485567909E-4	4.51830819043553E-6	2.191379472361232E-4	1.5814078666524356E-5	7.568166218979513E-5
26-27	1.8525063580785673E-4	6.777462285653295E-6	2.4059991114069198E-4	1.5814078666524356E-5	7.568166218979513E-5
28-29	2.067125997124255E-4	6.777462285653295E-6	2.49636527521563E-4	1.9202809809351002E-5	7.907039333262178E-5
30-31	2.315632947598209E-4	6.777462285653295E-6	2.49636527521563E-4	2.259154095217765E-5	8.132954742783954E-5
32-33	2.451182193311275E-4	6.777462285653295E-6	2.637562406166741E-4	2.259154095217765E-5	9.262531790392838E-5
34-35	2.654506061880874E-4	6.777462285653295E-6	2.914308782830917E-4	2.259154095217765E-5	1.0222672280860387E-4
36-37	2.987731290925494E-4	6.777462285653295E-6	3.0724495694961604E-4	2.259154095217765E-5	1.1295770476088825E-4
38-39	3.5355761590158023E-4	6.777462285653295E-6	3.17411150378096E-4	2.259154095217765E-5	1.2877178342741261E-4
40-41	3.817970420918023E-4	7.342250809457736E-6	3.298364979017937E-4	2.259154095217765E-5	1.366788227606748E-4
42-43	4.1681393056767764E-4	1.5814078666524356E-5	3.41697056901687E-4	2.259154095217765E-5	1.3893797685589254E-4
44-45	4.586082813292063E-4	1.5814078666524356E-5	3.6033507818723353E-4	2.259154095217765E-5	1.6039994076046132E-4
46-47	5.049209402811705E-4	2.767463766641762E-5	3.8066746504419343E-4	2.259154095217765E-5	1.6039994076046132E-4
48-49	5.608350041378101E-4	3.4452099952070913E-5	3.8066746504419343E-4	2.259154095217765E-5	1.7000134566513684E-4
50-51	6.043237204707522E-4	3.953519666631089E-5	3.930928125678911E-4	2.6545060618808737E-5	1.7508444237937678E-4
52-53	6.57978630232174E-4	3.953519666631089E-5	4.0834210271061103E-4	2.710984914261318E-5	1.7621401942698566E-4
54-55	7.099391744221827E-4	3.953519666631089E-5	4.247209699009398E-4	2.8239426190222062E-5	1.8525063580785673E-4
56-57	7.64158872707409E-4	3.953519666631089E-5	4.450533567578997E-4	2.9369003237830945E-5	2.067125997124255E-4
58-59	8.059532234689377E-4	3.953519666631089E-5	4.501364534721397E-4	2.9369003237830945E-5	2.0953654233144773E-4
60-61	8.641264414207951E-4	4.348871633294198E-5	5.009674206145395E-4	3.106336880924427E-5	2.1010133085525216E-4
62-63	9.217348708488481E-4	4.7442235999573065E-5	5.642237352806368E-4	3.2757734380657595E-5	2.1010133085525216E-4
64-65	9.799080888007055E-4	4.7442235999573065E-5	5.794730254233567E-4	3.2757734380657595E-5	2.1574921609329655E-4
66-67	0.0010250911707050609	4.7442235999573065E-5	5.8116739099477E-4	4.066477371391977E-5	2.1574921609329655E-4
68-69	0.0010691446755618072	4.970139009479083E-5	5.902040073756411E-4	4.461829338055086E-5	2.1857315871231874E-4
70-71	0.0011244939508946424	5.19605441900086E-5	5.981110467089034E-4	5.083096714239971E-5	2.259154095217765E-4
72-73	0.0011911389967035665	5.19605441900086E-5	6.223969532324942E-4	5.3090121237617474E-5	2.3382244885503868E-4
74-75	0.001263431927750535	5.3090121237617474E-5	6.223969532324942E-4	5.3090121237617474E-5	2.3551681442645202E-4
76-77	0.0013204755686547836	6.890419990414183E-5	6.235265302801031E-4	5.3090121237617474E-5	2.451182193311275E-4
78-79	0.0013752600554638144	6.890419990414183E-5	6.235265302801031E-4	5.3090121237617474E-5	2.490717389977586E-4
80-81	0.0014097121554158855	6.890419990414183E-5	6.246561073277121E-4	5.3090121237617474E-5	2.586731439024341E-4
82-83	0.0014435994668441517	6.890419990414183E-5	6.25785684375321E-4	5.3090121237617474E-5	2.6940412585471846E-4
84-85	0.0014769219897486138	6.890419990414183E-5	6.269152614229298E-4	5.3654909761421916E-5	2.767463766641762E-4
86-87	0.0015057262044626404	6.890419990414183E-5	6.404701859942364E-4	5.421969828522636E-5	2.9538439794972275E-4
88-89	0.0015136332437959026	6.890419990414183E-5	6.478124368036941E-4	5.421969828522636E-5	3.2136467004472704E-4
90	0.0015136332437959026	6.890419990414183E-5	6.506363794227163E-4	5.421969828522636E-5	3.3096607494940256E-4
>>END_MODULE
