##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290305_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89819288
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3148624936773	37.0	37.0	37.0	37.0	37.0
2	36.31831749768491	37.0	37.0	37.0	37.0	37.0
3	36.394659674879634	37.0	37.0	37.0	37.0	37.0
4	36.41545312628174	37.0	37.0	37.0	37.0	37.0
5	36.42957817701694	37.0	37.0	37.0	37.0	37.0
6	36.40390393653532	37.0	37.0	37.0	37.0	37.0
7	36.358350981361596	37.0	37.0	37.0	37.0	37.0
8	36.41440357443047	37.0	37.0	37.0	37.0	37.0
9	36.39018539091514	37.0	37.0	37.0	37.0	37.0
10-11	36.412259068453096	37.0	37.0	37.0	37.0	37.0
12-13	36.39048749751835	37.0	37.0	37.0	37.0	37.0
14-15	36.38202705971128	37.0	37.0	37.0	37.0	37.0
16-17	36.37907926301976	37.0	37.0	37.0	37.0	37.0
18-19	36.374218831483056	37.0	37.0	37.0	37.0	37.0
20-21	36.368313474050254	37.0	37.0	37.0	37.0	37.0
22-23	36.3540533075702	37.0	37.0	37.0	37.0	37.0
24-25	36.32541105758932	37.0	37.0	37.0	37.0	37.0
26-27	36.28205215788395	37.0	37.0	37.0	37.0	37.0
28-29	36.26882427524921	37.0	37.0	37.0	37.0	37.0
30-31	36.26015652673622	37.0	37.0	37.0	37.0	37.0
32-33	36.25425936353447	37.0	37.0	37.0	37.0	37.0
34-35	36.2414474049271	37.0	37.0	37.0	37.0	37.0
36-37	36.237123758560216	37.0	37.0	37.0	37.0	37.0
38-39	36.23068985983289	37.0	37.0	37.0	37.0	37.0
40-41	36.22868816155399	37.0	37.0	37.0	37.0	37.0
42-43	36.211282377171024	37.0	37.0	37.0	37.0	37.0
44-45	36.21172529620584	37.0	37.0	37.0	37.0	37.0
46-47	36.20757066741314	37.0	37.0	37.0	37.0	37.0
48-49	36.18270636431244	37.0	37.0	37.0	37.0	37.0
50-51	36.17677235303623	37.0	37.0	37.0	37.0	37.0
52-53	36.17548324532974	37.0	37.0	37.0	37.0	37.0
54-55	36.16592054725275	37.0	37.0	37.0	37.0	37.0
56-57	36.15265620460043	37.0	37.0	37.0	37.0	37.0
58-59	36.141579115214554	37.0	37.0	37.0	37.0	37.0
60-61	36.13964546923897	37.0	37.0	37.0	37.0	37.0
62-63	36.12886371557744	37.0	37.0	37.0	37.0	37.0
64-65	36.11305460765425	37.0	37.0	37.0	37.0	37.0
66-67	36.10950820180592	37.0	37.0	37.0	37.0	37.0
68-69	36.09564315253391	37.0	37.0	37.0	37.0	37.0
70-71	36.076138815562004	37.0	37.0	37.0	37.0	37.0
72-73	36.05394737428942	37.0	37.0	37.0	37.0	37.0
74-75	36.04960920355113	37.0	37.0	37.0	37.0	37.0
76-77	36.03785831859177	37.0	37.0	37.0	37.0	37.0
78-79	36.02607553795107	37.0	37.0	37.0	37.0	37.0
80-81	36.00025799874738	37.0	37.0	37.0	37.0	37.0
82-83	36.01091215191647	37.0	37.0	37.0	37.0	37.0
84-85	35.99487809623794	37.0	37.0	37.0	37.0	37.0
86-87	36.00648301030414	37.0	37.0	37.0	37.0	37.0
88-89	35.98349256929221	37.0	37.0	37.0	37.0	37.0
90-91	35.96616600771899	37.0	37.0	37.0	37.0	37.0
92-93	35.95528039304223	37.0	37.0	37.0	37.0	37.0
94-95	35.92859270504488	37.0	37.0	37.0	37.0	37.0
96-97	35.92752738916141	37.0	37.0	37.0	37.0	37.0
98-99	35.9070128364954	37.0	37.0	37.0	37.0	37.0
100-101	35.863596032358856	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25404.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.0
14	22.0
15	280.0
16	1211.0
17	3179.0
18	5472.0
19	7672.0
20	9888.0
21	13259.0
22	19894.0
23	35356.0
24	64395.0
25	113271.0
26	180929.0
27	283613.0
28	421159.0
29	604301.0
30	804399.0
31	1063761.0
32	1353054.0
33	1856882.0
34	2818445.0
35	6460252.0
36	5.2456382E7
37	2.1216806E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.1076336734674	15.468365916555793	17.9757150015026	45.448285408474206
2	22.95431869891943	20.674865305376073	30.754079498477658	25.61673649722684
3	25.106427616040666	21.62102209958011	22.778814683659387	30.493735600719834
4	24.955274234725128	26.190408522273458	20.46585357487615	28.38846366812527
5	26.440842852227792	30.209914338692133	22.89667982443255	20.45256298464753
6	21.1358448421721	34.94461048148892	22.308350087629574	21.611194588709402
7	18.76399176585345	20.74847102058755	39.829184802831335	20.658352410727662
8	20.683167018368422	24.205973760974633	28.349023191824514	26.76183602883243
9	21.24705890318897	22.46825123341922	30.91851068428025	25.36617917911156
10-11	23.21215774562107	30.843106196408655	23.20204291419224	22.742693143778034
12-13	22.158038612570707	25.654708975332458	27.488781917464163	24.698470494632673
14-15	22.395836973038143	26.309815552683357	26.923155800376968	24.371191673901528
16-17	23.06744157548427	26.44860532332625	26.346789917238954	24.137163183950523
18-19	22.861061347958326	26.923539864681565	26.023659170404205	24.191739616955896
20-21	23.064874882645093	26.79671531654203	26.154115685729863	23.984294115083017
22-23	22.81326467325428	26.896288327161795	26.423459860413534	23.86698713917039
24-25	22.6908698097202	26.823759668945435	26.524662940130966	23.960707581203394
26-27	22.91712216470845	26.943980445257466	26.283928144727298	23.85496924530679
28-29	22.810081694244314	26.796244260309976	26.2930211064764	24.100652938969315
30-31	22.730051280529786	26.79717459480799	26.380026360166692	24.09274776449553
32-33	22.985521958127396	26.889558249167877	26.227497659128634	23.89742213357609
34-35	22.98274847114879	26.770712256911068	26.201156070033687	24.045383201906457
36-37	22.791988188273493	26.712457770513904	26.406248820129314	24.089305221083293
38-39	23.215271873453048	26.817607523815155	26.207175904160035	23.75994469857176
40-41	23.16207970719623	26.7353638726557	26.065550493692708	24.03700592645536
42-43	22.90530799948162	26.749089467491842	26.362832480991784	23.98277005203475
44-45	23.093652053525744	26.74523689581727	26.346753492206854	23.814357558450133
46-47	23.053495705561453	26.605399194520967	26.31151084576312	24.02959425415446
48-49	22.917578386197274	26.548912224911497	26.348265938760168	24.18524345013106
50-51	23.197511180257987	26.622536524180994	26.172044289866424	24.007908005694592
52-53	23.291286720149756	26.712910274154062	26.043623901860286	23.9521791038359
54-55	22.83053598749367	26.643628089095717	26.409829978741605	24.11600594466901
56-57	23.107955161066254	26.72240784570286	26.3165702782394	23.853066714991485
58-59	23.1482782945496	26.551127455465224	26.307634486592875	23.992959763392303
60-61	23.095635229223987	26.53158645471716	26.32521119606163	24.04756711999722
62-63	23.184477282688558	26.58118620618485	26.341816847989147	23.892519663137442
64-65	23.186296770337016	26.646933398954314	26.214516113670083	23.952253717038587
66-67	23.130949831454306	26.60834998559804	26.31321399314267	23.94748618980498
68-69	23.343684901178744	26.68789730507939	26.215723881346086	23.752693912395777
70-71	23.43996818980979	26.662853924003333	26.10460638736039	23.79257149882649
72-73	23.20227104872852	26.614884555860442	26.249414697330316	23.933429698080726
74-75	23.27356311393513	26.665337355466306	26.35802267508672	23.70307685551185
76-77	23.3439602451731	26.525958023328656	26.28095585844772	23.849125873050525
78-79	23.102603506686144	26.633345870821497	26.2980353914669	23.966015231025462
80-81	23.439500439121556	26.717609711997813	26.134075714033184	23.708814134847444
82-83	23.417700032456672	26.726891355088426	26.042423658481223	23.812984953973682
84-85	23.18407160212137	26.75117629758157	26.2032483096013	23.861503790695757
86-87	23.35224496775386	26.66225347366229	26.203583366105054	23.78191819247879
88-89	23.487014220249055	26.41835150901648	26.189424284671308	23.905209986063152
90-91	23.270036699863823	26.52510680207652	26.382549655343396	23.82230684271626
92-93	23.456478449750254	26.584492143883715	26.274223650282224	23.684805756083808
94-95	23.438246147434125	26.53300967072296	26.30082499240342	23.72791918943949
96-97	23.32702220577993	26.39350743918742	26.426673930204675	23.852796424827975
98-99	23.59025723517819	25.539062641234157	26.786430752720758	24.084249370866896
100-101	24.264841125527074	12.037818681836002	33.160667365787546	30.53667282684937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25581.0
1	12827.5
2	65.5
3	52.0
4	59.5
5	70.0
6	77.5
7	86.0
8	97.0
9	128.0
10	176.0
11	255.0
12	387.0
13	557.5
14	826.5
15	1228.5
16	1795.0
17	2557.0
18	3552.0
19	4842.0
20	6318.0
21	8375.5
22	11911.5
23	17061.0
24	23016.5
25	31156.0
26	43374.0
27	61847.0
28	85634.5
29	115241.0
30	161820.0
31	223631.5
32	313461.5
33	435414.0
34	587174.0
35	784908.5
36	1061449.0
37	1429349.5
38	1886036.5
39	2448097.0
40	3008605.0
41	3550054.0
42	4122251.5
43	4653437.5
44	5074872.5
45	5397934.5
46	5569882.0
47	5551575.5
48	5330256.5
49	4998231.5
50	4624647.5
51	4210926.0
52	3754645.0
53	3219807.0
54	2750152.5
55	2400488.0
56	2075925.0
57	1722251.5
58	1376907.5
59	1071191.5
60	882806.0
61	784334.0
62	712160.5
63	633163.5
64	525005.5
65	441582.5
66	388257.0
67	341643.0
68	278121.5
69	205314.0
70	147924.0
71	110462.0
72	82303.0
73	62000.0
74	48944.5
75	36700.5
76	23367.5
77	14330.5
78	9469.0
79	5786.0
80	4369.0
81	3336.0
82	1958.5
83	1039.5
84	743.0
85	581.0
86	465.0
87	386.5
88	341.0
89	303.5
90	282.5
91	269.0
92	253.5
93	245.0
94	267.0
95	411.0
96	764.5
97	1931.5
98	6505.5
99	7326.5
100	2373.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06469991167153319
2	0.040090498156698816
3	0.03010377904576576
4	0.028287910721358646
5	0.028303497573928665
6	0.02828345733491007
7	0.02828345733491007
8	0.02828345733491007
9	0.028285684028134358
10-11	0.02828345733491007
12-13	0.028303497573928665
14-15	0.02837976181686054
16-17	0.02829180743450115
18-19	0.028509466697175332
20-21	0.028312404346825817
22-23	0.028529506936193928
24-25	0.029612236516504117
26-27	0.030576951355927025
28-29	0.031738171872393375
30-31	0.032580975257786506
32-33	0.03348278601362326
34-35	0.03446475772553441
36-37	1.2195462660854045E-4
38-39	1.670630060140065E-5
40-41	2.394596596122148E-5
42-43	3.77570841854503E-4
44-45	6.961191873219477E-5
46-47	1.6428614556668046E-4
48-49	1.5482071468861809E-4
50-51	6.90577219748811E-5
52-53	9.801887917126997E-5
54-55	3.1188294516233464E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.126750151435162E-6
64-65	6.12690214620425E-6
66-67	6.127070529995555E-6
68-69	0.0
70-71	2.7852465401639626E-6
72-73	7.242034578085695E-6
74-75	0.0
76-77	4.4572387005098855E-6
78-79	0.0
80-81	2.2290858127320154E-6
82-83	6.521092546297583E-5
84-85	1.1149448241122694E-5
86-87	6.133719695221714E-6
88-89	2.7610010861164717E-4
90-91	5.579926068657954E-5
92-93	1.0049542626053465E-5
94-95	6.334981238483805E-4
96-97	3.413455814842965E-5
98-99	2.3374225419502072E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	31552.0
36-37	1012.0
38-39	988.0
40-41	1022.0
42-43	981.0
44-45	1121.0
46-47	1053.0
48-49	1256.0
50-51	1336.0
52-53	1354.0
54-55	1425.0
56-57	1509.0
58-59	1764.0
60-61	2057.0
62-63	2213.0
64-65	2429.0
66-67	2784.0
68-69	3668.0
70-71	4722.0
72-73	5322.0
74-75	6210.0
76-77	7717.0
78-79	9860.0
80-81	13120.0
82-83	17178.0
84-85	21273.0
86-87	25530.0
88-89	31938.0
90-91	47854.0
92-93	50855.0
94-95	79759.0
96-97	711280.0
98-99	8310425.0
100-101	8.0416721E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.941315392708596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.02525329410525	12.852930057764548
2	16.817749410532375	7.716425868451247
3	8.177908088244777	5.628359061150181
4	4.677895448891768	4.292682994685688
5	2.9095853289846825	3.3374857347117706
6	1.988389323386376	2.7369759954780783
7	1.3890500981573404	2.230664547867027
8	1.1058341325207162	2.0295431684944054
9	0.8513555229480579	1.7578094006948182
>10	5.262802831797822	22.703268902204872
>50	0.4488140680719942	7.135236038417641
>100	0.29489669150912706	13.116004322773886
>500	0.03019541285808602	4.764633205581912
>1k	0.019445625631827546	8.285237096163685
>5k	7.159048262556127E-4	1.057094791360687
>10k+	1.0881753359085314E-4	0.35564881419970557
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.2266932242883065E-6	0.0	0.0
2	2.2266932242883065E-6	0.0	7.793426285009074E-6	0.0	1.1133466121441532E-6
3	5.566733060720766E-6	0.0	7.793426285009074E-6	0.0	4.453386448576613E-6
4	4.564721109791028E-5	0.0	2.004023901859476E-5	1.1133466121441532E-6	1.2246812733585687E-5
5	5.678067721935182E-5	0.0	3.228705175218045E-5	1.1133466121441532E-6	1.3360159345729839E-5
6	7.793426285009072E-5	0.0	4.119382464933367E-5	2.2266932242883065E-6	1.3360159345729839E-5
7	8.016095607437904E-5	0.0	6.123406366792844E-5	2.2266932242883065E-6	1.4473505957873993E-5
8	8.906772897153226E-5	0.0	6.234741028007259E-5	2.2266932242883065E-6	1.4473505957873993E-5
9	9.018107558367641E-5	1.1133466121441532E-6	6.45741035043609E-5	2.2266932242883065E-6	2.1153585630738913E-5
10-11	1.024278883172621E-4	3.3400398364324597E-6	7.62642429318745E-5	2.2266932242883065E-6	2.171025893681099E-5
12-13	1.1745806758120818E-4	3.3400398364324597E-6	8.684103574724395E-5	3.3400398364324597E-6	2.8390338609675907E-5
14-15	1.4417838627266784E-4	4.453386448576613E-6	1.0910796799012702E-4	4.453386448576613E-6	3.1173705140036294E-5
16-17	1.553118523941094E-4	7.236752978936996E-6	1.3415826676337045E-4	4.453386448576613E-6	3.228705175218045E-5
18-19	1.6366195198519055E-4	1.002011950929738E-5	1.3972499982409123E-4	4.453386448576613E-6	3.785378481290121E-5
20-21	1.6588864520947883E-4	1.1133466121441532E-5	1.503017926394607E-4	5.566733060720766E-6	4.2307171261477825E-5
22-23	1.7590876471877621E-4	1.1133466121441532E-5	1.6366195198519052E-4	5.566733060720766E-6	4.3977191179694054E-5
24-25	1.9817569696165926E-4	1.948356571252268E-5	1.6811533843376716E-4	6.123406366792843E-6	4.453386448576613E-5
26-27	2.1320587622560537E-4	2.171025893681099E-5	1.7535209141270416E-4	6.6800796728649195E-6	4.7873904322198594E-5
28-29	2.521730076506507E-4	2.2266932242883065E-5	1.9594900373737099E-4	6.6800796728649195E-6	5.789402383149597E-5
30-31	2.861300793210474E-4	2.2266932242883065E-5	1.981756969616593E-4	6.6800796728649195E-6	6.401743019828881E-5
32-33	2.9893356536070517E-4	2.4493625467171373E-5	2.0151573679809173E-4	7.793426285009074E-6	6.45741035043609E-5
34-35	3.117370514003629E-4	2.4493625467171373E-5	2.1765926267418198E-4	7.793426285009074E-6	6.568745011650505E-5
36-37	3.3456065694931805E-4	2.4493625467171373E-5	2.2266932242883064E-4	7.793426285009074E-6	7.904760946223489E-5
38-39	3.551575692739849E-4	2.8947011915747986E-5	2.3213276863205597E-4	7.793426285009074E-6	9.29644421140368E-5
40-41	3.7686782821079587E-4	2.8947011915747986E-5	2.5773974071137146E-4	7.793426285009074E-6	1.0131454170511795E-4
42-43	4.019181269840393E-4	2.9503685221820062E-5	2.7109990005710135E-4	9.463446203225302E-6	1.0354123492940625E-4
44-45	4.247417325329945E-4	3.3957071670396675E-5	2.816766928724708E-4	1.002011950929738E-5	1.0354123492940625E-4
46-47	4.475653380819496E-4	3.5627091588612904E-5	2.9448017891212856E-4	1.1133466121441532E-5	1.0465458154155041E-4
48-49	4.837491029766346E-4	3.8410458118973285E-5	3.072836649517863E-4	1.1133466121441532E-5	1.074379480719108E-4
50-51	5.238295810138241E-4	4.008047803718952E-5	3.0839701156393045E-4	1.1133466121441532E-5	1.1467470105084779E-4
52-53	5.73930178560311E-4	4.008047803718952E-5	3.1340707131857916E-4	1.2246812733585687E-5	1.169013942751361E-4
54-55	6.10113943454996E-4	4.0637151343261596E-5	3.2955059719466933E-4	1.2246812733585687E-5	1.169013942751361E-4
56-57	6.501944214921855E-4	4.2307171261477825E-5	3.495908362132641E-4	1.2246812733585687E-5	1.3026155362086595E-4
58-59	7.019650389568886E-4	4.342051787362198E-5	3.6016762902863354E-4	1.2246812733585687E-5	1.45291732884812E-4
60-61	7.353654373212133E-4	4.564721109791028E-5	4.002481070658231E-4	1.2246812733585687E-5	1.497451193333886E-4
62-63	7.793426285009073E-4	4.7873904322198594E-5	4.4812201138802173E-4	1.2246812733585687E-5	1.5419850578196522E-4
64-65	8.194231065380968E-4	4.7873904322198594E-5	4.6983227032483267E-4	1.2246812733585687E-5	1.59765238842686E-4
66-67	8.890072697971063E-4	5.01005975464869E-5	4.7762569660984177E-4	1.2246812733585687E-5	1.6533197190340677E-4
68-69	9.279744012221518E-4	5.399731068899143E-5	4.831924296705625E-4	1.2246812733585687E-5	1.7256872488234377E-4
70-71	9.713949190957738E-4	5.789402383149597E-5	4.831924296705625E-4	1.2246812733585687E-5	1.81475497779497E-4
72-73	0.0010220521899483325	5.789402383149597E-5	4.932125491798599E-4	1.2246812733585687E-5	1.881555774523619E-4
74-75	0.0010788328671676845	5.95640437497122E-5	4.94325895792004E-4	1.2246812733585687E-5	1.981756969616593E-4
76-77	0.0011278201181020272	6.17907369740005E-5	4.965525890162923E-4	1.2246812733585687E-5	2.070824698588125E-4
78-79	0.0011578804766299196	6.45741035043609E-5	4.982226089345086E-4	1.2246812733585687E-5	2.1598924275596573E-4
80-81	0.001191280874994244	6.45741035043609E-5	4.998926288527248E-4	1.2246812733585687E-5	2.2433934234704686E-4
82-83	0.001221341233522136	6.568745011650505E-5	5.060160352195176E-4	1.5030179263946071E-5	2.2767938218347936E-4
84-85	0.0012686584645382627	6.90274899529375E-5	5.126961148923826E-4	1.5586852570018147E-5	2.287927287956235E-4
86-87	0.001293152090005434	7.236752978936996E-5	5.188195212591754E-4	1.67001991821623E-5	2.3491613516241633E-4
88-89	0.0012959354565357944	7.459422301365827E-5	5.199328678713196E-4	1.67001991821623E-5	2.4159621483528125E-4
90	0.0012959354565357944	7.904760946223489E-5	5.254996009320404E-4	1.67001991821623E-5	2.4604960128385786E-4
>>END_MODULE
