##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290309_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77072011
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08903723817457	37.0	37.0	37.0	37.0	37.0
2	36.14113849968181	37.0	37.0	37.0	37.0	37.0
3	36.241291965250525	37.0	37.0	37.0	37.0	37.0
4	36.2157081900977	37.0	37.0	37.0	37.0	37.0
5	36.16631191834348	37.0	37.0	37.0	37.0	37.0
6	36.09460067157194	37.0	37.0	37.0	37.0	37.0
7	36.05428158608707	37.0	37.0	37.0	37.0	37.0
8	36.1223750993081	37.0	37.0	37.0	37.0	37.0
9	36.071221730544956	37.0	37.0	37.0	37.0	37.0
10-11	36.093099121288006	37.0	37.0	37.0	37.0	37.0
12-13	36.07732731925212	37.0	37.0	37.0	37.0	37.0
14-15	36.064321417537684	37.0	37.0	37.0	37.0	37.0
16-17	36.050330716036456	37.0	37.0	37.0	37.0	37.0
18-19	36.05604633827448	37.0	37.0	37.0	37.0	37.0
20-21	36.04462137363978	37.0	37.0	37.0	37.0	37.0
22-23	36.01745738800042	37.0	37.0	37.0	37.0	37.0
24-25	36.00305637542013	37.0	37.0	37.0	37.0	37.0
26-27	35.972391053867796	37.0	37.0	37.0	37.0	37.0
28-29	35.95576903397525	37.0	37.0	37.0	37.0	37.0
30-31	35.937241335249446	37.0	37.0	37.0	37.0	37.0
32-33	35.91790314125838	37.0	37.0	37.0	37.0	37.0
34-35	35.8984329732878	37.0	37.0	37.0	37.0	37.0
36-37	35.88523627734223	37.0	37.0	37.0	37.0	37.0
38-39	35.87535605969441	37.0	37.0	37.0	37.0	37.0
40-41	35.880556534872106	37.0	37.0	37.0	37.0	37.0
42-43	35.843086238440435	37.0	37.0	37.0	37.0	37.0
44-45	35.82843057980629	37.0	37.0	37.0	37.0	37.0
46-47	35.83202636081538	37.0	37.0	37.0	37.0	37.0
48-49	35.81098290534952	37.0	37.0	37.0	37.0	37.0
50-51	35.793784675273194	37.0	37.0	37.0	37.0	37.0
52-53	35.78270395471425	37.0	37.0	37.0	37.0	37.0
54-55	35.77875439877195	37.0	37.0	37.0	37.0	37.0
56-57	35.74681831284467	37.0	37.0	37.0	37.0	37.0
58-59	35.767473708076345	37.0	37.0	37.0	37.0	37.0
60-61	35.73515317855303	37.0	37.0	37.0	37.0	37.0
62-63	35.72269367999709	37.0	37.0	37.0	37.0	37.0
64-65	35.71188501979035	37.0	37.0	37.0	37.0	37.0
66-67	35.693795145991885	37.0	37.0	37.0	37.0	37.0
68-69	35.66122272165074	37.0	37.0	37.0	37.0	37.0
70-71	35.67029728173905	37.0	37.0	37.0	37.0	37.0
72-73	35.64808704301997	37.0	37.0	37.0	37.0	37.0
74-75	35.66152354370405	37.0	37.0	37.0	37.0	37.0
76-77	35.636640010388646	37.0	37.0	37.0	37.0	37.0
78-79	35.590827678609216	37.0	37.0	37.0	37.0	37.0
80-81	35.589232107619935	37.0	37.0	37.0	37.0	37.0
82-83	35.55720354228639	37.0	37.0	37.0	37.0	37.0
84-85	35.514962689832274	37.0	37.0	37.0	37.0	37.0
86-87	35.48194193429545	37.0	37.0	37.0	37.0	37.0
88-89	35.512319012153654	37.0	37.0	37.0	37.0	37.0
90-91	35.45658021987153	37.0	37.0	37.0	37.0	37.0
92-93	35.502736600560766	37.0	37.0	37.0	37.0	37.0
94-95	35.45753904544115	37.0	37.0	37.0	37.0	37.0
96-97	35.43733723878028	37.0	37.0	37.0	37.0	37.0
98-99	35.43477367886969	37.0	37.0	37.0	37.0	37.0
100-101	35.42119102287184	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10499.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	42.0
12	2117.0
13	16044.0
14	33730.0
15	36818.0
16	36273.0
17	39261.0
18	40844.0
19	40303.0
20	44815.0
21	62748.0
22	87419.0
23	117040.0
24	143841.0
25	175551.0
26	211590.0
27	272258.0
28	351644.0
29	472573.0
30	632791.0
31	909182.0
32	1335212.0
33	2232988.0
34	4169503.0
35	1.0911395E7
36	4.5404981E7
37	9280549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.785351391794983	16.4187605446464	13.58955969117796	42.20632837238066
2	23.535198737494472	19.786545569522566	29.316833998002785	27.361421694980177
3	25.038294698946455	19.793473286403625	22.587139394261374	32.58109262038854
4	25.78945432840829	24.358472492649856	20.465697883585225	29.386375295356633
5	26.552102932771753	28.207092414234346	22.867702864431173	22.37310178856272
6	22.496449082528365	33.007826617382015	22.462046183377847	22.033678116711776
7	19.665730872948632	22.336807904554256	37.105359188509944	20.892102033987168
8	20.71946786993147	24.37104476532354	28.568413891388317	26.341073473356673
9	21.4888996727705	22.53117872901326	30.83477521178147	25.145146386434774
10-11	23.14281916019928	30.24545614612446	23.892227251345	22.71949744233126
12-13	22.575699802142434	25.699985113188045	27.217837816177354	24.506477268492166
14-15	22.58674157031007	26.24131644787473	26.825719859518944	24.346222122296254
16-17	23.33917311206526	26.200875779295647	26.272663850005245	24.18728725863385
18-19	23.149276852640448	26.65309196273853	25.778982005430677	24.41864917919035
20-21	23.15740607706014	26.450172143091166	26.038224393118263	24.354197386730426
22-23	23.04715871205063	26.49862851924966	26.150415426828765	24.303797341870943
24-25	23.03895110370724	26.311561256321745	26.287808328196533	24.36167931177448
26-27	23.25227088853575	26.50694682285834	26.014913619756253	24.225868668849657
28-29	23.046837652304667	26.38962564684515	26.11297083083464	24.45056587001554
30-31	23.02531413922071	26.105701607316696	26.27216145987322	24.596822793589375
32-33	23.220511392833227	26.2959591453845	26.142815851538487	24.34071361024378
34-35	23.15314701030585	26.38150440415547	26.032559963133572	24.43278862240511
36-37	22.910931535397914	26.30057163007172	26.28226290264495	24.506233931885422
38-39	23.29211292885783	26.4040268883177	26.13115939160606	24.172700791218404
40-41	23.439825255756393	26.28251522920036	25.727021638645446	24.550637876397793
42-43	23.23869135253693	26.34696486845824	26.048372243974978	24.365971535029857
44-45	23.411852096096155	26.278972508444664	26.183821999376818	24.125353396082367
46-47	23.28656687755335	26.07531793554739	26.18258657717779	24.455528609721462
48-49	23.091842802545177	25.88726814886211	26.321090313055052	24.699798735537666
50-51	23.318611900659626	25.914098676414454	26.13956753025774	24.627721892668184
52-53	23.550831203822046	26.19442505116253	25.87442335236468	24.38032039265075
54-55	23.16144002131114	26.19446506304795	26.132252409764376	24.511842505876533
56-57	23.245595378726176	26.3340142851782	26.044663511263693	24.375726824831936
58-59	23.27643108890322	26.21438180590312	26.019301024690566	24.48988608050309
60-61	23.3604772451119	26.05167978915915	26.009828093390514	24.57801487233844
62-63	23.4319233159869	25.93910886589387	26.014776295067715	24.614191523051513
64-65	23.478884575416494	26.132253832957623	25.76029937993744	24.628562211688436
66-67	23.51079559170688	26.11412385019454	25.841591194967616	24.53348936313096
68-69	23.459651234808014	26.164598421706987	25.99514095258737	24.380609390897632
70-71	23.82781816709002	26.138398207680485	25.773274494582342	24.260509130647154
72-73	23.495221518451178	26.125655469867194	25.82148252728421	24.557640484397417
74-75	23.59902666913256	26.21659396088208	25.831274259907428	24.353105110077937
76-77	23.604600542544027	26.159875115495844	25.81749597563604	24.418028366324087
78-79	23.44930884651569	26.273134341080862	25.653394065479553	24.624162746923897
80-81	23.667784776618824	26.216598925527617	25.632223381079182	24.483392916774374
82-83	23.692342898913033	26.28801625334122	25.47104515845815	24.548595689287602
84-85	23.566283754509524	26.307499869752178	25.6215119749305	24.5047044008078
86-87	23.607561664784914	26.224990136699244	25.62631635131376	24.541131847202085
88-89	23.813639330865563	25.98790891277144	25.54477649510835	24.653675261254644
90-91	23.686396074303957	26.00860778671575	25.841213807859543	24.463782331120747
92-93	24.03012550529359	25.904062798664874	25.748144329967214	24.317667366074325
94-95	23.819086752652964	25.97067239798367	25.776292568539557	24.43394828082381
96-97	23.752318468661027	25.765405842344624	25.89832929784539	24.583946391148963
98-99	24.020388359596513	24.98668580219505	26.246859957286357	24.74606588092208
100-101	24.969781935618883	11.580651428282515	32.10332358565625	31.346243050442357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10771.0
1	5493.0
2	368.5
3	786.5
4	1319.5
5	1845.5
6	2384.5
7	2803.5
8	3262.0
9	3715.5
10	4070.0
11	4476.0
12	4904.5
13	5365.5
14	5735.5
15	6031.5
16	6396.0
17	6944.5
18	7635.5
19	8514.0
20	9578.0
21	10963.5
22	13822.0
23	18392.0
24	23556.5
25	30110.5
26	37399.5
27	49389.0
28	66193.5
29	85852.0
30	118267.5
31	159114.0
32	225207.5
33	323340.0
34	435947.5
35	573853.5
36	779850.5
37	1063196.0
38	1405775.0
39	1860817.0
40	2312903.0
41	2722918.5
42	3196127.5
43	3664719.5
44	4050457.5
45	4361461.5
46	4517327.5
47	4520358.0
48	4420171.0
49	4251815.5
50	4054396.0
51	3841240.0
52	3464954.0
53	2917644.0
54	2471558.5
55	2184380.5
56	1949433.0
57	1676559.5
58	1410716.5
59	1146819.5
60	947541.5
61	846871.5
62	794428.5
63	734092.5
64	615352.0
65	504200.5
66	445701.5
67	394802.0
68	317871.5
69	240100.5
70	176949.0
71	130706.0
72	102199.5
73	81990.0
74	71484.5
75	56769.0
76	37309.5
77	30017.5
78	25004.0
79	21169.5
80	19353.0
81	15064.0
82	9662.0
83	6159.5
84	5527.0
85	5137.0
86	4786.0
87	4325.0
88	3852.5
89	3399.0
90	2979.0
91	2574.0
92	2247.5
93	1972.0
94	1733.0
95	1615.0
96	1528.0
97	1497.0
98	1645.5
99	2168.5
100	10929.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06039806071752818
2	0.02943351251078683
3	0.014381355638949139
4	0.014188029945137931
5	0.014164675163335234
6	0.015109246338466503
7	0.01363919257277457
8	0.014274961632959078
9	0.013622325230361514
10-11	0.01365800614700452
12-13	0.013643085036408353
14-15	0.01364568001216421
16-17	0.014476720997976813
18-19	0.013674224745478615
20-21	0.013674224745478615
22-23	0.015401181111000204
24-25	0.015619159074492036
26-27	0.01710997264623081
28-29	0.018492445980162628
30-31	0.019841833373207298
32-33	0.021703079734094393
34-35	0.022866926360595417
36-37	2.0050985047792268E-4
38-39	5.321050100152545E-4
40-41	6.391857337014793E-4
42-43	0.001095393064247561
44-45	8.55304408776895E-4
46-47	5.808128343879823E-4
48-49	5.95100163307296E-4
50-51	0.001021488032638191
52-53	3.595395106210373E-4
54-55	2.5311059618176046E-5
56-57	8.761707434036821E-5
58-59	1.817282617302348E-5
60-61	5.062545543438562E-5
62-63	3.2453005111601438E-6
64-65	2.012136023173212E-5
66-67	7.140020514706942E-6
68-69	1.6876873426259953E-5
70-71	2.5316047812848613E-5
72-73	0.0
74-75	1.1685048145416996E-5
76-77	4.219736041747757E-5
78-79	5.713079401279925E-5
80-81	2.648891341912318E-4
82-83	0.0015900454971164602
84-85	1.610239711710993E-4
86-87	0.0012921471284172827
88-89	5.461048535604575E-4
90-91	0.002264451651538231
92-93	9.703647172946641E-4
94-95	0.0010802551987606232
96-97	0.0018365058712130227
98-99	0.0016687528502487188
100-101	3.22632407075045E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	18178.0
36-37	1090.0
38-39	1311.0
40-41	1149.0
42-43	1373.0
44-45	1380.0
46-47	1354.0
48-49	1340.0
50-51	1463.0
52-53	1560.0
54-55	1634.0
56-57	1645.0
58-59	1728.0
60-61	1859.0
62-63	1907.0
64-65	1893.0
66-67	2141.0
68-69	2170.0
70-71	2314.0
72-73	2384.0
74-75	2435.0
76-77	2719.0
78-79	2865.0
80-81	2990.0
82-83	3122.0
84-85	3507.0
86-87	3624.0
88-89	4488.0
90-91	12558.0
92-93	8318.0
94-95	27608.0
96-97	539958.0
98-99	6917535.0
100-101	6.9490411E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.970400727394445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.36164116138676	13.481398456062484
2	15.25589607944043	6.703563006415841
3	7.322596095922663	4.82641111776825
4	4.014448148502369	3.5279613808777492
5	2.5534622868994017	2.8050294842734456
6	1.7237205752301161	2.272249906791631
7	1.1553349994638062	1.776822103882271
8	0.8681396615835262	1.5258701001867738
9	0.7161466965813682	1.4160626913143177
>10	4.245669376698342	17.640119605011108
>50	0.4079446347329812	6.204919877032152
>100	0.301112426914529	13.339518024751046
>500	0.039202708470473704	6.003514758837195
>1k	0.03219901883733157	13.752374185366614
>5k	0.0019491253992419111	2.8571942967828523
>10k+	5.370039365258327E-4	1.8669910046461378
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2974878779275657E-6	0.0	5.189951511710263E-6	0.0	2.5949757558551314E-6
2	2.5949757558551314E-6	0.0	5.189951511710263E-6	0.0	5.189951511710263E-6
3	2.5949757558551314E-6	0.0	6.487439389637828E-6	0.0	7.784927267565393E-6
4	1.0379903023420526E-5	0.0	1.1677390901348092E-5	0.0	1.1677390901348092E-5
5	1.5569854535130787E-5	0.0	1.4272366657203222E-5	0.0	1.1677390901348092E-5
6	2.984222119233401E-5	0.0	1.6867342413058353E-5	0.0	1.2974878779275656E-5
7	3.113970907026157E-5	0.0	3.632966058197184E-5	0.0	2.2057293924768617E-5
8	3.762714845989941E-5	0.0	3.762714845989941E-5	1.2974878779275657E-6	2.5949757558551312E-5
9	3.892463633782697E-5	0.0	4.670956360539237E-5	1.2974878779275657E-6	2.8544733314406445E-5
10-11	4.800705148331993E-5	0.0	6.0981930262595584E-5	1.2974878779275657E-6	3.373468482611671E-5
12-13	5.5143234811921545E-5	0.0	7.849801661461773E-5	1.2974878779275657E-6	4.087086815471832E-5
14-15	6.746936965223342E-5	0.0	9.796033478353121E-5	1.2974878779275657E-6	4.4114587849537235E-5
16-17	8.174173630943663E-5	1.2974878779275657E-6	1.1677390901348092E-4	1.2974878779275657E-6	4.4114587849537235E-5
18-19	9.212163933285716E-5	2.5949757558551314E-6	1.2391009234208252E-4	1.2974878779275657E-6	4.4114587849537235E-5
20-21	1.0185279841731391E-4	2.5949757558551314E-6	1.3039753173172035E-4	1.9462318168913483E-6	5.2548259056066406E-5
22-23	1.1482767719658957E-4	2.5949757558551314E-6	1.5569854535130788E-4	2.5949757558551314E-6	5.644072268984911E-5
24-25	1.3558748324343062E-4	3.892463633782697E-6	1.6023975292405438E-4	2.5949757558551314E-6	5.708946662881289E-5
26-27	1.6932216806954734E-4	5.189951511710263E-6	1.7061965594747489E-4	5.189951511710263E-6	5.968444238466802E-5
28-29	2.186267074307948E-4	5.189951511710263E-6	1.8294579078778677E-4	5.189951511710263E-6	6.487439389637829E-5
30-31	2.620925513413683E-4	5.838695450674046E-6	1.9008197411638837E-4	5.189951511710263E-6	7.006434540808855E-5
32-33	2.95827236167485E-4	9.08241514549296E-6	1.9397443775017107E-4	6.487439389637828E-6	7.136183328601611E-5
34-35	3.3345438462738437E-4	9.08241514549296E-6	2.1668047561390348E-4	6.487439389637828E-6	7.849801661461773E-5
36-37	3.7692022853795786E-4	9.08241514549296E-6	2.2187042712561373E-4	6.487439389637828E-6	8.952666357700203E-5
38-39	4.197373285095675E-4	9.08241514549296E-6	2.4327897711141856E-4	6.487439389637828E-6	1.0120405447835012E-4
40-41	4.6644689211495984E-4	1.1028646962384309E-5	2.620925513413683E-4	6.487439389637828E-6	1.1093521356280686E-4
42-43	5.365112375230485E-4	1.2974878779275656E-5	2.8155486951028175E-4	6.487439389637828E-6	1.1547642113555334E-4
44-45	5.955469359687527E-4	1.362362271823944E-5	2.860960770830282E-4	7.13618332860161E-6	1.1547642113555334E-4
46-47	6.532851465365294E-4	1.4272366657203222E-5	2.964759801064488E-4	8.433671206529176E-6	1.2001762870829983E-4
48-49	7.285394434563281E-4	1.4272366657203222E-5	3.0361216343505036E-4	9.08241514549296E-6	1.2391009234208252E-4
50-51	7.940625812916702E-4	1.4272366657203222E-5	3.0555839525194174E-4	9.08241514549296E-6	1.2585632415897387E-4
52-53	8.615319509439036E-4	1.5569854535130787E-5	3.1918201797018116E-4	9.731159084456743E-6	1.2780255597586522E-4
54-55	9.53004846337797E-4	1.6867342413058353E-5	3.282644331156741E-4	1.0379903023420526E-5	1.3039753173172037E-4
56-57	0.0010250154235627769	1.6867342413058353E-5	3.3799559220013084E-4	1.0379903023420526E-5	1.401286908161771E-4
58-59	0.001126219478041127	1.7516086352022138E-5	3.516192149183703E-4	1.0379903023420526E-5	1.5245482565648898E-4
60-61	0.001201473774960926	1.816483029098592E-5	3.827589239886319E-4	1.2974878779275658E-5	1.5894226504612678E-4
62-63	0.0013182476839744067	1.88135742299497E-5	4.164936088147486E-4	1.4272366657203222E-5	1.621859847409457E-4
64-65	0.0014317778732930687	1.9462318168913485E-5	4.307659754719518E-4	1.4272366657203222E-5	1.660784483747284E-4
66-67	0.0015375231353441654	1.9462318168913485E-5	4.320634633498794E-4	1.4272366657203222E-5	1.6672719231369218E-4
68-69	0.001642619653456298	1.9462318168913485E-5	4.385509027395172E-4	1.4272366657203222E-5	1.7580960745918514E-4
70-71	0.001744472451873612	1.9462318168913485E-5	4.385509027395172E-4	1.4272366657203222E-5	1.9721815744499E-4
72-73	0.0018443790184740345	1.9462318168913485E-5	4.6709563605392367E-4	1.4272366657203222E-5	2.0694931652944675E-4
74-75	0.0019721815744499	1.9462318168913485E-5	4.6709563605392367E-4	1.5569854535130787E-5	2.1019303622426565E-4
76-77	0.002082468044073743	1.9462318168913485E-5	4.6969061180977877E-4	1.5569854535130787E-5	2.1343675591908455E-4
78-79	0.0021765359152234915	1.9462318168913485E-5	4.7358307544356147E-4	1.6218598474094568E-5	2.186267074307948E-4
80-81	0.0022569801636550006	1.9462318168913485E-5	4.800705148331993E-4	1.816483029098592E-5	2.225191710645775E-4
82-83	0.0023023922393824656	2.075980604684105E-5	4.83962978466982E-4	1.816483029098592E-5	2.2900661045421536E-4
84-85	0.002384133975691902	2.2706037863732402E-5	4.923966496735111E-4	1.816483029098592E-5	2.3225033014903426E-4
86-87	0.0024250048438466203	2.465226968062375E-5	4.98884089063149E-4	1.816483029098592E-5	2.3679153772178073E-4
88-89	0.002431492283236258	2.465226968062375E-5	4.995328330021128E-4	1.816483029098592E-5	2.426302331724548E-4
90	0.002431492283236258	2.724724543647888E-5	4.995328330021128E-4	1.816483029098592E-5	2.426302331724548E-4
>>END_MODULE
