##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290308_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74571019
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04863260618713	37.0	37.0	37.0	37.0	37.0
2	36.02634621366781	37.0	37.0	37.0	37.0	37.0
3	36.12594335072718	37.0	37.0	37.0	37.0	37.0
4	36.10482463703493	37.0	37.0	37.0	37.0	37.0
5	36.06902385228235	37.0	37.0	37.0	37.0	37.0
6	35.986874458561445	37.0	37.0	37.0	37.0	37.0
7	35.94051724035044	37.0	37.0	37.0	37.0	37.0
8	36.01389112035602	37.0	37.0	37.0	37.0	37.0
9	35.95895707151326	37.0	37.0	37.0	37.0	37.0
10-11	35.992443324396575	37.0	37.0	37.0	37.0	37.0
12-13	35.97228493685999	37.0	37.0	37.0	37.0	37.0
14-15	35.96396676569486	37.0	37.0	37.0	37.0	37.0
16-17	35.944610439076875	37.0	37.0	37.0	37.0	37.0
18-19	35.95209634992382	37.0	37.0	37.0	37.0	37.0
20-21	35.94012555333326	37.0	37.0	37.0	37.0	37.0
22-23	35.90969297335202	37.0	37.0	37.0	37.0	37.0
24-25	35.89718896693634	37.0	37.0	37.0	37.0	37.0
26-27	35.86400406436715	37.0	37.0	37.0	37.0	37.0
28-29	35.843416119873595	37.0	37.0	37.0	37.0	37.0
30-31	35.823622384722945	37.0	37.0	37.0	37.0	37.0
32-33	35.803806784509675	37.0	37.0	37.0	37.0	37.0
34-35	35.78527004572648	37.0	37.0	37.0	37.0	37.0
36-37	35.769979102693604	37.0	37.0	37.0	37.0	37.0
38-39	35.75977079131676	37.0	37.0	37.0	37.0	37.0
40-41	35.76495420045013	37.0	37.0	37.0	37.0	37.0
42-43	35.7202789226596	37.0	37.0	37.0	37.0	37.0
44-45	35.70371129739411	37.0	37.0	37.0	37.0	37.0
46-47	35.71320909807868	37.0	37.0	37.0	37.0	37.0
48-49	35.69131261056559	37.0	37.0	37.0	37.0	37.0
50-51	35.67072714567331	37.0	37.0	37.0	37.0	37.0
52-53	35.65859492186782	37.0	37.0	37.0	37.0	37.0
54-55	35.6550783079329	37.0	37.0	37.0	37.0	37.0
56-57	35.62042060114544	37.0	37.0	37.0	37.0	37.0
58-59	35.63908362721737	37.0	37.0	37.0	37.0	37.0
60-61	35.60712972644134	37.0	37.0	37.0	37.0	37.0
62-63	35.590128792028715	37.0	37.0	37.0	37.0	37.0
64-65	35.577231264783954	37.0	37.0	37.0	37.0	37.0
66-67	35.55896089227587	37.0	37.0	37.0	37.0	37.0
68-69	35.523300775922365	37.0	37.0	37.0	37.0	37.0
70-71	35.53390650762012	37.0	37.0	37.0	37.0	37.0
72-73	35.51224374772197	37.0	37.0	37.0	37.0	37.0
74-75	35.52639701119981	37.0	37.0	37.0	37.0	37.0
76-77	35.500700362019536	37.0	37.0	37.0	37.0	37.0
78-79	35.45167481535536	37.0	37.0	37.0	37.0	37.0
80-81	35.44786521820832	37.0	37.0	37.0	37.0	37.0
82-83	35.41472370037059	37.0	37.0	37.0	37.0	37.0
84-85	35.3695998295027	37.0	37.0	37.0	37.0	37.0
86-87	35.3287690009946	37.0	37.0	37.0	37.0	37.0
88-89	35.358364984195994	37.0	37.0	37.0	37.0	37.0
90-91	35.29162839854298	37.0	37.0	37.0	37.0	37.0
92-93	35.34253118289685	37.0	37.0	37.0	37.0	37.0
94-95	35.29162386323078	37.0	37.0	37.0	37.0	37.0
96-97	35.27025304373362	37.0	37.0	37.0	37.0	37.0
98-99	35.267218093857394	37.0	37.0	37.0	37.0	37.0
100-101	35.24874549046329	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16120.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	60.0
12	2221.0
13	14606.0
14	26869.0
15	27320.0
16	24912.0
17	27158.0
18	31148.0
19	36518.0
20	46124.0
21	65167.0
22	91995.0
23	127320.0
24	161934.0
25	203068.0
26	244738.0
27	316734.0
28	405416.0
29	545272.0
30	729437.0
31	1042633.0
32	1529380.0
33	2534409.0
34	4654253.0
35	1.1645584E7
36	4.2368955E7
37	7651668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.95332728940882	17.193173329156558	11.556614999516976	44.29688438191764
2	22.549123771201433	19.678007259756793	30.53206458764558	27.240804381396195
3	23.716572575132837	19.321914299516678	22.914296136595755	34.04721698875473
4	24.770837367296362	23.972465449291068	21.256578880656342	30.000118302756228
5	25.840541068831698	27.675202788209706	23.71039084196479	22.77386530099381
6	22.245086977465455	32.57880937632584	23.28942203133142	21.88668161487728
7	19.38995550794857	22.854504915774136	36.91580525435689	20.8397343219204
8	19.969774025064833	24.51214729471636	29.409976355660422	26.108102324558384
9	20.63376199151613	22.63508861128197	31.955840539891028	24.775308857310876
10-11	22.413690448206932	30.26457520126351	24.786295317981303	22.535439032548254
12-13	21.95655290525812	26.020658352196563	27.741241195588245	24.281547546957068
14-15	21.98372157704804	26.420737832776304	27.294766374003537	24.300774216172115
16-17	22.848325754337523	26.38623474554624	26.778554881857104	23.986884618259133
18-19	22.618937600974316	26.841696415457882	26.088957324707916	24.45040865885989
20-21	22.630258658585262	26.640488092114555	26.446671757280377	24.282581492019805
22-23	22.52237173284093	26.645519960872104	26.634449380857678	24.19765892542929
24-25	22.68242990006225	26.3387992955465	26.746703096235468	24.232067708155782
26-27	22.890211227819282	26.648384289042493	26.47845043677352	23.982954046364704
28-29	22.607989642543497	26.481744586527878	26.622021148575225	24.2882446223534
30-31	22.642710088291945	26.073466826338677	26.765851791628176	24.517971293741205
32-33	22.949555984277076	26.25290005103046	26.554056371697293	24.243487592995173
34-35	22.870718721306773	26.42792919934372	26.39212507201321	24.309227007336297
36-37	22.445382780004223	26.401259321087572	26.66870910265089	24.484648796257318
38-39	22.775864582366246	26.51294296723811	26.79483039408479	23.916362056310852
40-41	23.13810662203326	26.404796880419468	26.089098433467072	24.3679980640802
42-43	22.80174733522457	26.591034087083283	26.3591858961214	24.24803268157075
44-45	23.02837211167164	26.365001043470905	26.604308086220968	24.00231875863649
46-47	22.848735681556033	26.04864024846199	26.690551638689275	24.412072431292703
48-49	22.64274180157189	25.86714245059038	26.83302941435984	24.657086333477896
50-51	22.871955797600847	25.811951831949102	26.64338492640839	24.672707444041656
52-53	23.114752039596294	26.244149849507757	26.32719670856649	24.313901402329456
54-55	22.722942131207233	26.197014821398103	26.60324348014421	24.476799567250456
56-57	22.839054408333784	26.3816210826772	26.432018202860164	24.347306306128853
58-59	22.754632293523233	26.29317646983998	26.433769819827795	24.51842141680899
60-61	22.912082908198812	26.143403592466584	26.362469949627677	24.582043549706928
62-63	22.967728235370288	25.955339036526855	26.46169097082327	24.61524175727959
64-65	23.021216121814593	26.187481812074658	26.201663126839808	24.589638939270937
66-67	23.06554261899429	26.073067777636588	26.30028808041565	24.561101522953468
68-69	22.92233737921746	26.208676236585305	26.49337780345545	24.375608580741783
70-71	23.466960328952077	26.119035998623126	26.272066895178526	24.14193677724627
72-73	23.16710538255887	25.943950135124304	26.318751479601282	24.570193002715545
74-75	23.37878151822027	26.10457340872003	26.26762986440905	24.249015208650654
76-77	23.299088390438293	26.12563349225195	26.28833369635903	24.286944420950732
78-79	22.98715297202597	26.287425007842145	26.018122643283014	24.70729937684887
80-81	23.16540936482028	26.175704905894325	26.042246611842764	24.616639117442634
82-83	23.23986845888343	26.29778308527279	25.747846441792337	24.71450201405144
84-85	23.187070257757586	26.34619294375831	25.94280097654741	24.52393582193669
86-87	23.1960477531965	26.268635250666584	25.932527750395533	24.60278924574138
88-89	23.310499579923256	26.022796824373124	25.882485727130838	24.78421786857279
90-91	23.232913304477528	25.96896254265866	26.34476947241578	24.453354680448037
92-93	23.662661749369583	25.8185183049741	26.20370623343541	24.315113712220914
94-95	23.389488691697544	25.919356288441985	26.238288926220367	24.452866093640104
96-97	23.38242352368211	25.73187200416833	26.338533316897607	24.547171155251952
98-99	23.62530439387946	24.870274437469657	26.71657928736366	24.787841881287214
100-101	24.58834946769747	11.450732624550392	32.41289895543434	31.54801895231779
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16315.0
1	8242.0
2	265.0
3	545.0
4	910.0
5	1363.0
6	1761.5
7	2051.0
8	2362.0
9	2732.0
10	3097.5
11	3397.0
12	3693.5
13	3992.5
14	4284.5
15	4708.0
16	5258.0
17	5778.0
18	6558.0
19	7614.5
20	8825.0
21	11380.5
22	15429.0
23	20054.5
24	25481.5
25	32430.5
26	38852.5
27	49686.0
28	66502.0
29	85363.0
30	114371.0
31	153974.5
32	219492.0
33	318049.5
34	431347.0
35	562046.5
36	761494.5
37	1049304.5
38	1400037.0
39	1888383.0
40	2378331.5
41	2794732.5
42	3281061.5
43	3776161.5
44	4163302.5
45	4489650.0
46	4609108.5
47	4529662.0
48	4385157.5
49	4212275.0
50	3997572.0
51	3747904.0
52	3336567.5
53	2728868.0
54	2244128.0
55	1941576.0
56	1695157.0
57	1427796.0
58	1211808.0
59	996583.0
60	811528.0
61	713359.5
62	657439.5
63	598496.0
64	503284.0
65	406108.5
66	346201.5
67	308082.0
68	254245.0
69	194326.0
70	143615.0
71	102752.0
72	77702.0
73	63035.0
74	55253.0
75	42116.5
76	25616.5
77	20153.5
78	16021.5
79	12752.5
80	11268.5
81	8787.5
82	5801.0
83	3994.0
84	3701.5
85	3490.0
86	3296.5
87	3022.5
88	2718.0
89	2457.0
90	2186.5
91	1947.5
92	1775.0
93	1568.5
94	1413.0
95	1363.5
96	1326.5
97	1339.5
98	1500.5
99	1980.0
100	10768.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.068399226246325
2	0.03684407209186722
3	0.022350505898276647
4	0.022184221460082233
5	0.02214265035053363
6	0.023022348668723436
7	0.0216290459970783
8	0.02226065866151031
9	0.021619658972341523
10-11	0.021654525064220997
12-13	0.02164044452711582
14-15	0.021631728004145954
16-17	0.022430966110306202
18-19	0.02168268613843134
20-21	0.021667264597792342
22-23	0.023552045064584675
24-25	0.02515655579280739
26-27	0.027544883086551356
28-29	0.029789723002175954
30-31	0.031671150960133725
32-33	0.03399175757541948
34-35	0.03559090428950689
36-37	1.824438667174576E-4
38-39	5.272180935507992E-4
40-41	6.412599303393167E-4
42-43	0.0010504507761986046
44-45	8.358161915157828E-4
46-47	5.708619145536033E-4
48-49	5.527618831344252E-4
50-51	9.81441208377786E-4
52-53	3.57566059239304E-4
54-55	3.153094004687604E-5
56-57	8.184829113313712E-5
58-59	2.079802700523171E-5
60-61	4.293890908307274E-5
62-63	7.380313407155835E-6
64-65	1.7444839116782824E-5
66-67	1.0735598225174799E-5
68-69	1.7445891550580978E-5
70-71	2.080153647932237E-5
72-73	6.710400043719599E-7
74-75	9.394894128703191E-6
76-77	2.8856826613166736E-5
78-79	5.1004718117626854E-5
80-81	2.6845681365775655E-4
82-83	0.0015376533243729286
84-85	1.5169176319632428E-4
86-87	0.0012001681524184556
88-89	4.94726713332754E-4
90-91	0.0021549483885161687
92-93	9.23228531921872E-4
94-95	0.0010475654829993721
96-97	0.0017642052305861283
98-99	0.0015595142228107517
100-101	3.0877264776610965E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	27268.0
36-37	1201.0
38-39	1445.0
40-41	1328.0
42-43	1466.0
44-45	1511.0
46-47	1564.0
48-49	1576.0
50-51	1558.0
52-53	1710.0
54-55	1823.0
56-57	1813.0
58-59	1789.0
60-61	1926.0
62-63	1910.0
64-65	2139.0
66-67	2330.0
68-69	2319.0
70-71	2542.0
72-73	2571.0
74-75	2745.0
76-77	2837.0
78-79	2954.0
80-81	3257.0
82-83	3371.0
84-85	3544.0
86-87	3901.0
88-89	4688.0
90-91	12590.0
92-93	8089.0
94-95	29733.0
96-97	540054.0
98-99	6705886.0
100-101	6.7185581E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.31417437069845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.92800136021533	12.783303924309635
2	14.528141690236088	5.9025440715533914
3	6.931516153355652	4.22424083377739
4	3.945291354522985	3.2058134607595594
5	2.416651378025734	2.4546138743201733
6	1.6375697022788496	1.995952588575918
7	1.1295693536710725	1.6062388169989918
8	0.846978066100201	1.3764528098333153
9	0.6538182701934875	1.1953600512713043
>10	4.158729719145268	16.182713796207636
>50	0.4182894578230044	5.901576953807788
>100	0.31949436431160266	13.26789774904891
>500	0.04363993761808482	6.142765841435128
>1k	0.03809157530427771	15.365189552378103
>5k	0.0029938526225223973	4.037050067214897
>10k+	0.001223764575629593	4.3582856085080595
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTGAACTTGTTTCAGGAAACATGAGGTCTTGGAACTTGATCTTTCTCT	84681	0.11355752024791294	No Hit
CTTTTATCGTTGAACTTGTTTCAGGAAACATGAGGTCTTGGAACTTGATC	83188	0.11155540197191083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6820070676518446E-6
2	0.0	4.023010601477767E-6	0.0	0.0	2.6820070676518446E-6
3	1.3410035338259223E-6	4.023010601477767E-6	0.0	0.0	4.023010601477767E-6
4	4.023010601477767E-6	5.364014135303689E-6	0.0	0.0	4.023010601477767E-6
5	1.609204240591107E-5	5.364014135303689E-6	0.0	1.3410035338259223E-6	5.364014135303689E-6
6	3.084308127799622E-5	5.364014135303689E-6	0.0	1.3410035338259223E-6	8.046021202955535E-6
7	3.084308127799622E-5	5.364014135303689E-6	6.705017669129612E-6	1.3410035338259223E-6	8.046021202955535E-6
8	4.559412015008136E-5	5.364014135303689E-6	1.3410035338259224E-5	1.3410035338259223E-6	8.046021202955535E-6
9	4.559412015008136E-5	5.364014135303689E-6	1.743304593973699E-5	1.3410035338259223E-6	1.0728028270607378E-5
10-11	6.302716608981836E-5	5.364014135303689E-6	2.3467561841953642E-5	2.6820070676518446E-6	1.3410035338259224E-5
12-13	8.582422616485903E-5	5.364014135303689E-6	2.5479067142692527E-5	4.023010601477767E-6	2.279706007504068E-5
14-15	1.052687774053349E-4	5.364014135303689E-6	2.9502077744170293E-5	4.023010601477767E-6	2.6820070676518448E-5
16-17	1.227018233450719E-4	5.364014135303689E-6	3.5536593646386945E-5	4.023010601477767E-6	3.0172579511083254E-5
18-19	1.367823604502441E-4	6.0345159022166506E-6	4.6935123683907283E-5	4.023010601477767E-6	3.151358304490918E-5
20-21	1.4952189402159035E-4	6.705017669129612E-6	5.3640141353036895E-5	4.023010601477767E-6	3.218408481182214E-5
22-23	1.5957942052528476E-4	6.705017669129612E-6	6.034515902216651E-5	4.693512368390729E-6	3.486609187947398E-5
24-25	1.7365995763045695E-4	6.705017669129612E-6	6.101566078907947E-5	5.364014135303689E-6	3.486609187947398E-5
26-27	1.951160141716717E-4	6.705017669129612E-6	6.168616255599244E-5	5.364014135303689E-6	3.486609187947398E-5
28-29	2.2394759014892902E-4	9.387024736781457E-6	6.503867139055723E-5	5.364014135303689E-6	3.6877597180212866E-5
30-31	2.682007067651845E-4	9.387024736781457E-6	6.906168199203501E-5	5.364014135303689E-6	4.023010601477767E-5
32-33	3.030667986446585E-4	1.0728028270607378E-5	7.24141908265998E-5	6.705017669129612E-6	4.6935123683907283E-5
34-35	3.2921636755426395E-4	1.2069031804433301E-5	7.77782049619035E-5	8.046021202955535E-6	4.8276127217733205E-5
36-37	3.8687951950877863E-4	1.2069031804433301E-5	7.911920849572942E-5	8.046021202955535E-6	5.4981144886862816E-5
38-39	4.4186066439564145E-4	1.2069031804433301E-5	8.180121556338127E-5	9.387024736781457E-6	6.637967492438316E-5
40-41	4.914777951472005E-4	1.2069031804433301E-5	8.716522969868496E-5	9.387024736781457E-6	8.046021202955534E-5
42-43	5.538344594701059E-4	1.4751038872085147E-5	9.185874206707568E-5	1.139853003752034E-5	8.314221909720719E-5
44-45	6.155206220260984E-4	1.542154063899811E-5	9.387024736781457E-5	1.743304593973699E-5	8.314221909720719E-5
46-47	6.886053146196111E-4	1.810354770664995E-5	1.0057526503694418E-4	1.810354770664995E-5	8.716522969868496E-5
48-49	7.61690007213124E-4	1.8774049473562915E-5	1.0728028270607379E-4	2.4138063608866602E-5	9.454074913472753E-5
50-51	8.307516892051589E-4	1.8774049473562915E-5	1.0862128623989972E-4	2.4138063608866602E-5	9.990476327003121E-5
52-53	9.071888906332364E-4	1.9444551240475875E-5	1.139853003752034E-4	2.4138063608866602E-5	1.019162685707701E-4
54-55	9.883196044297047E-4	2.0115053007388836E-5	1.2203132157815894E-4	2.4138063608866602E-5	1.052687774053349E-4
56-57	0.0010654273076246953	2.0115053007388836E-5	1.3141834631494038E-4	2.4138063608866602E-5	1.227018233450719E-4
58-59	0.001156615547924858	2.0115053007388836E-5	1.3812336398407E-4	2.4138063608866602E-5	1.327593498487663E-4
60-61	0.0012672483394654966	2.0785554774301796E-5	1.4415787988628667E-4	2.5479067142692527E-5	1.3611185868333113E-4
62-63	0.001362459590367137	2.1456056541214757E-5	1.5823841699145883E-4	2.5479067142692527E-5	1.367823604502441E-4
64-65	0.0014730923819077757	2.1456056541214757E-5	1.6092042405911069E-4	2.5479067142692527E-5	1.401348692848089E-4
66-67	0.0015964647070197606	2.1456056541214757E-5	1.6092042405911069E-4	2.5479067142692527E-5	1.4616938518702553E-4
68-69	0.0017205075338986584	2.279706007504068E-5	1.6092042405911069E-4	2.5479067142692527E-5	1.5220390108924218E-4
70-71	0.001815718784800299	2.279706007504068E-5	1.6092042405911069E-4	2.6149568909605487E-5	1.6695493996132733E-4
72-73	0.0019370796046115448	2.279706007504068E-5	1.7097795056280512E-4	2.6820070676518448E-5	1.7030744879589215E-4
74-75	0.0020785554774301798	2.279706007504068E-5	1.7164845232971805E-4	2.816107421034437E-5	1.7231895409663102E-4
76-77	0.0021657207071288645	2.279706007504068E-5	1.7298945586354398E-4	2.816107421034437E-5	1.7298945586354398E-4
78-79	0.0022562384456621147	2.279706007504068E-5	1.743304593973699E-4	2.9502077744170293E-5	1.7567146293119584E-4
80-81	0.0023286526364887142	2.279706007504068E-5	1.7567146293119584E-4	2.9502077744170293E-5	1.7701246646502177E-4
82-83	0.002384974784909403	2.279706007504068E-5	1.783534699988477E-4	2.9502077744170293E-5	1.796944735326736E-4
84-85	0.002446660947465395	2.279706007504068E-5	1.8170597883341249E-4	2.9502077744170293E-5	1.796944735326736E-4
86-87	0.002474151519908827	2.279706007504068E-5	1.8237648060032545E-4	2.9502077744170293E-5	1.8237648060032545E-4
88-89	0.0024808565375779566	2.279706007504068E-5	1.8371748413415138E-4	2.9502077744170293E-5	1.8572898943489024E-4
90	0.0024808565375779566	2.5479067142692527E-5	1.8371748413415138E-4	2.9502077744170293E-5	1.917635053371069E-4
>>END_MODULE
