##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290297_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74837690
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.88989892659701	37.0	37.0	37.0	37.0	37.0
2	36.02407837548166	37.0	37.0	37.0	37.0	37.0
3	36.0831042486747	37.0	37.0	37.0	37.0	37.0
4	36.087194473800565	37.0	37.0	37.0	37.0	37.0
5	36.05433223553533	37.0	37.0	37.0	37.0	37.0
6	35.99336994501033	37.0	37.0	37.0	37.0	37.0
7	35.94723627893913	37.0	37.0	37.0	37.0	37.0
8	36.00456884759538	37.0	37.0	37.0	37.0	37.0
9	35.9505139990291	37.0	37.0	37.0	37.0	37.0
10-11	35.98650307084572	37.0	37.0	37.0	37.0	37.0
12-13	35.96412039174378	37.0	37.0	37.0	37.0	37.0
14-15	35.95433007753179	37.0	37.0	37.0	37.0	37.0
16-17	35.93559312159421	37.0	37.0	37.0	37.0	37.0
18-19	35.942140631278164	37.0	37.0	37.0	37.0	37.0
20-21	35.93203075883288	37.0	37.0	37.0	37.0	37.0
22-23	35.90263332553423	37.0	37.0	37.0	37.0	37.0
24-25	35.88847875983345	37.0	37.0	37.0	37.0	37.0
26-27	35.855621772932864	37.0	37.0	37.0	37.0	37.0
28-29	35.836362212676526	37.0	37.0	37.0	37.0	37.0
30-31	35.81703324220724	37.0	37.0	37.0	37.0	37.0
32-33	35.790059627708985	37.0	37.0	37.0	37.0	37.0
34-35	35.772336425670005	37.0	37.0	37.0	37.0	37.0
36-37	35.76460559171716	37.0	37.0	37.0	37.0	37.0
38-39	35.749129783241564	37.0	37.0	37.0	37.0	37.0
40-41	35.750002543488	37.0	37.0	37.0	37.0	37.0
42-43	35.71277598668405	37.0	37.0	37.0	37.0	37.0
44-45	35.70075980665189	37.0	37.0	37.0	37.0	37.0
46-47	35.702607733144404	37.0	37.0	37.0	37.0	37.0
48-49	35.67409609599238	37.0	37.0	37.0	37.0	37.0
50-51	35.650700758275654	37.0	37.0	37.0	37.0	37.0
52-53	35.634993801771415	37.0	37.0	37.0	37.0	37.0
54-55	35.628597846328475	37.0	37.0	37.0	37.0	37.0
56-57	35.595767325722974	37.0	37.0	37.0	37.0	37.0
58-59	35.61792533580822	37.0	37.0	37.0	37.0	37.0
60-61	35.57805076870981	37.0	37.0	37.0	37.0	37.0
62-63	35.566423971484355	37.0	37.0	37.0	37.0	37.0
64-65	35.55394330808953	37.0	37.0	37.0	37.0	37.0
66-67	35.5312899713694	37.0	37.0	37.0	37.0	37.0
68-69	35.49517586496947	37.0	37.0	37.0	37.0	37.0
70-71	35.49582461441513	37.0	37.0	37.0	37.0	37.0
72-73	35.47406733813272	37.0	37.0	37.0	37.0	37.0
74-75	35.489105909984694	37.0	37.0	37.0	37.0	37.0
76-77	35.45979579700395	37.0	37.0	37.0	37.0	37.0
78-79	35.409914869659154	37.0	37.0	37.0	37.0	37.0
80-81	35.403040908578895	37.0	37.0	37.0	37.0	37.0
82-83	35.36610248979693	37.0	37.0	37.0	37.0	37.0
84-85	35.31946606003348	37.0	37.0	37.0	37.0	37.0
86-87	35.27948676162827	37.0	37.0	37.0	37.0	37.0
88-89	35.307810403314136	37.0	37.0	37.0	37.0	37.0
90-91	35.2465974936699	37.0	37.0	37.0	31.0	37.0
92-93	35.29108181913634	37.0	37.0	37.0	37.0	37.0
94-95	35.238360690003816	37.0	37.0	37.0	31.0	37.0
96-97	35.21004696745702	37.0	37.0	37.0	31.0	37.0
98-99	35.205058237862175	37.0	37.0	37.0	25.0	37.0
100-101	35.18377615741149	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25083.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	33.0
12	990.0
13	8713.0
14	20442.0
15	26095.0
16	29030.0
17	32987.0
18	32807.0
19	32848.0
20	40086.0
21	61327.0
22	94451.0
23	138908.0
24	181088.0
25	221993.0
26	263618.0
27	336358.0
28	427981.0
29	569255.0
30	755515.0
31	1077271.0
32	1566062.0
33	2581727.0
34	4724758.0
35	1.1795209E7
36	4.2246575E7
37	7546480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.107473819470396	17.10419544277396	11.110481102101149	45.677849635654496
2	22.256715501010508	19.751846785671955	31.764277734832746	26.227159978484792
3	23.50697168493351	19.844152366696463	23.16381971865386	33.485056229716164
4	24.860534402039924	24.91649053335261	20.877186878280522	29.34578818632694
5	26.094160529957293	28.721254176974863	23.094689200294653	22.08989609277319
6	22.106936640795695	32.99062895585832	23.08601320379641	21.816421199549577
7	19.37439730233652	22.656458385288154	37.05488459676835	20.91425971560697
8	19.773967355073	24.398793222177893	29.61124451048128	26.21599491226782
9	20.68218823977339	22.754260157706575	31.56527524251728	24.99827636000275
10-11	22.530571127728223	30.734397056366802	24.0221076716748	22.71292414423018
12-13	22.0413673414947	26.208078396857022	27.21241602232437	24.53813823932391
14-15	22.101647822211852	26.737525861538963	26.829034521548422	24.331791794700763
16-17	22.85028666123848	26.64085445224976	26.119307092961215	24.38955179355055
18-19	22.73643420017738	26.926536466904583	25.878306640074783	24.458722692843253
20-21	22.86858222522861	26.797369650722082	26.19367567810192	24.14037244594739
22-23	22.738389711919233	26.821417663464164	26.191863846117062	24.248328778499538
24-25	22.561976829399537	26.821818935540016	26.28399124713911	24.332212987921338
26-27	22.662233702138067	26.931047539350438	26.27982751608389	24.126891242427597
28-29	22.581289268317615	26.876116765810785	26.13656268488551	24.406031280986088
30-31	22.57320556814969	26.693386693443244	26.217787515662287	24.515620222744783
32-33	22.76554231989674	26.771698181881014	26.15645957588576	24.306299922336486
34-35	22.730176783816155	26.772189536717818	26.006017284401512	24.491616395064515
36-37	22.606765279361337	26.665798531264638	26.138385551588634	24.58905063778539
38-39	22.7045419777304	26.851417325936072	26.151461588751356	24.29257910758217
40-41	22.871835332763688	26.698401374934484	26.02935323467897	24.400410057622864
42-43	22.784308354855153	26.572387062648268	26.261938729500066	24.381365852996513
44-45	22.86393913245803	26.56474372085923	26.306152619761065	24.26516452692168
46-47	22.885719689769772	26.6041466412329	26.124580556515898	24.38555311248142
48-49	22.740096881793207	26.516317202793125	26.19835875548262	24.545227159931045
50-51	22.877125454102814	26.610108711207165	26.099066927192556	24.41369890749747
52-53	23.17617233897014	26.640721858121747	25.84938654236973	24.333719260538388
54-55	22.94841213782444	26.573784331025873	26.05965272271949	24.418150808430187
56-57	22.90700602484491	26.656607672047855	26.12242742061855	24.31395888248869
58-59	23.04703290679917	26.562825086714366	26.024014645106224	24.36612736138024
60-61	23.03745854080929	26.42919235799245	26.019745024422313	24.513604076775948
62-63	23.140061549477767	26.376793320573032	26.109558755237195	24.373586374712005
64-65	23.247245292784662	26.48052325195288	25.895901732612536	24.376329722649924
66-67	23.208933818392588	26.487770830281736	25.925704970152346	24.377590381173327
68-69	23.20192325429401	26.543084588979166	25.944646999333848	24.310345157392977
70-71	23.415617847470273	26.513440776211105	25.745525225972717	24.325416150345898
72-73	23.145909758985077	26.579476624294056	25.865706103958487	24.408907512762383
74-75	23.18325301683949	26.592385574290105	25.86058052033288	24.363780888537523
76-77	23.336488929863442	26.527111098594126	25.712205917173304	24.424194054369135
78-79	23.133009636090303	26.46623247390229	25.90252334864583	24.49823454136158
80-81	23.24249169456976	26.524630840943015	25.84096974136756	24.39190772311966
82-83	23.375106478126078	26.478296560876547	25.711627959009338	24.43496900198804
84-85	23.36774267323211	26.41183929398676	25.802088300433006	24.41832973234812
86-87	23.42276884609889	26.394230896950287	25.82674825612002	24.356252000830803
88-89	23.58728168119616	26.33126438366157	25.683134167649836	24.398319767492442
90-91	23.44886858005286	26.303873033836034	25.82107399608772	24.426184390023387
92-93	23.570975402873806	26.385258850360454	25.784952524942188	24.258813221823548
94-95	23.618440857296214	26.3074409197814	25.72589351087562	24.348224712046765
96-97	23.43261417381333	26.22611374609047	25.789712824375943	24.551559255720253
98-99	23.64440618090463	25.419459356372215	26.267519393084843	24.668615069638307
100-101	24.50267496442214	12.005301388244014	32.39206620183182	31.09995744550203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25193.0
1	12696.5
2	370.0
3	780.0
4	1242.0
5	1714.0
6	2110.5
7	2446.5
8	2859.0
9	3199.5
10	3562.0
11	4008.0
12	4466.5
13	4931.0
14	5456.5
15	6150.5
16	6935.0
17	7901.5
18	9056.5
19	10662.5
20	12674.5
21	15195.5
22	18554.5
23	23341.5
24	29914.0
25	38160.5
26	49346.0
27	66110.0
28	89858.5
29	119617.0
30	160595.5
31	216810.0
32	295686.0
33	399878.5
34	536859.0
35	727521.0
36	966941.5
37	1245564.0
38	1595641.5
39	2012627.0
40	2434604.0
41	2854310.5
42	3295346.5
43	3675293.0
44	3985081.5
45	4205658.0
46	4325847.5
47	4359155.0
48	4233419.5
49	3962005.5
50	3646202.5
51	3337433.5
52	3018421.0
53	2674816.0
54	2303122.0
55	1991623.0
56	1718335.0
57	1452960.0
58	1235084.5
59	1066313.5
60	923103.0
61	807707.5
62	718895.5
63	634149.0
64	572891.0
65	514335.0
66	448047.5
67	390430.0
68	323622.0
69	253232.0
70	204340.0
71	160410.5
72	122532.0
73	97863.5
74	85880.0
75	66843.5
76	42025.0
77	34490.0
78	25666.5
79	17260.5
80	15609.0
81	11676.5
82	5377.0
83	1570.5
84	1254.5
85	1118.5
86	1029.5
87	941.5
88	842.0
89	781.5
90	748.5
91	691.5
92	671.5
93	683.0
94	683.0
95	695.5
96	732.0
97	796.5
98	944.5
99	1338.5
100	7597.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07912857812687699
2	0.04896463266036138
3	0.034268828981760396
4	0.034091111043112104
5	0.03403766203900735
6	0.034977028286148334
7	0.0335352414004227
8	0.03417529322457708
9	0.033517870474088655
10-11	0.033549939876551506
12-13	0.03353791385062794
14-15	0.03353724573807663
16-17	0.034330295336480854
18-19	0.03358802229197614
20-21	0.03356062967737246
22-23	0.03566518421399698
24-25	0.037843231131265545
26-27	0.04104215402693483
28-29	0.04402995335639034
30-31	0.04676186557869437
32-33	0.050031608404802454
34-35	0.05277688287813266
36-37	1.9720182640978334E-4
38-39	5.073899948910039E-4
40-41	6.223894988892787E-4
42-43	0.0010556194877225037
44-45	8.390404743831884E-4
46-47	6.077376475334119E-4
48-49	5.836862969505841E-4
50-51	9.79526662495109E-4
52-53	3.7644443417710267E-4
54-55	3.3433093449327625E-5
56-57	7.489274389480704E-5
58-59	1.9392563393286667E-5
60-61	4.213024790835524E-5
62-63	6.68760144506227E-6
64-65	1.605086776809594E-5
66-67	8.694559392836177E-6
68-69	1.80586471420676E-5
70-71	3.076787451309003E-5
72-73	0.0
74-75	8.696061208711837E-6
76-77	3.8799673758963835E-5
78-79	4.950553702372269E-5
80-81	2.57577177950184E-4
82-83	0.0015294920286000023
84-85	1.666082266565615E-4
86-87	0.0012285630223019721
88-89	5.326819944170644E-4
90-91	0.002195855756945846
92-93	9.559022478831251E-4
94-95	0.0010491081007815955
96-97	0.0017694958461017987
98-99	0.0016465298251689532
100-101	3.344659149645282E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	40781.0
36-37	1857.0
38-39	2131.0
40-41	2133.0
42-43	2386.0
44-45	2289.0
46-47	2282.0
48-49	2167.0
50-51	2449.0
52-53	2389.0
54-55	2587.0
56-57	2666.0
58-59	2691.0
60-61	2963.0
62-63	2855.0
64-65	2952.0
66-67	2946.0
68-69	3041.0
70-71	3354.0
72-73	3393.0
74-75	3587.0
76-77	3652.0
78-79	4195.0
80-81	4207.0
82-83	4338.0
84-85	4743.0
86-87	4988.0
88-89	6216.0
90-91	14533.0
92-93	10489.0
94-95	30728.0
96-97	527972.0
98-99	6779310.0
100-101	6.734842E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.016396591170615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.62491444573809	19.42399182937142
2	15.2079428529771	9.43391173727593
3	7.1441705091766945	6.647592774827091
4	3.9915027866718633	4.952081337047088
5	2.4195880952999445	3.75234519755491
6	1.6137928938313562	3.0032424246651743
7	1.1428298164821038	2.481252397695656
8	0.864515790222436	2.145133168709467
9	0.6831567695653856	1.9070155168904657
>10	3.8349142425908265	21.79781843499705
>50	0.28020754841634227	5.985748848351617
>100	0.16935084953678856	10.047853196789843
>500	0.015019021945814472	3.1858294132716236
>1k	0.0077794629550683725	4.384347984545112
>5k	2.460270235797055E-4	0.505626139716836
>10k+	6.888756660231755E-5	0.34620959829064274
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.672450205237495E-6	0.0	0.0	0.0	2.672450205237495E-6
2	5.34490041047499E-6	5.34490041047499E-6	0.0	1.3362251026187474E-6	6.681125513093737E-6
3	6.681125513093737E-6	5.34490041047499E-6	1.3362251026187474E-6	2.672450205237495E-6	1.7370926334043714E-5
4	1.6034701231424966E-5	5.34490041047499E-6	1.068980082094998E-5	6.681125513093737E-6	1.870715143666246E-5
5	2.0043376539281208E-5	5.34490041047499E-6	1.7370926334043714E-5	6.681125513093737E-6	2.8060727154993695E-5
6	3.875052797594367E-5	5.34490041047499E-6	1.870715143666246E-5	9.35357571833123E-6	2.9396952257612442E-5
7	3.875052797594367E-5	5.34490041047499E-6	2.8060727154993695E-5	1.068980082094998E-5	3.206940246284993E-5
8	4.275920328379992E-5	5.34490041047499E-6	2.8060727154993695E-5	1.2026025923568727E-5	3.206940246284993E-5
9	4.8104103694274906E-5	5.34490041047499E-6	3.3405627565468684E-5	1.4698476128806221E-5	3.607807777070618E-5
10-11	5.411711665605927E-5	8.017350615712483E-6	3.875052797594367E-5	1.4698476128806221E-5	3.674619032201555E-5
12-13	6.547503002831862E-5	1.068980082094998E-5	5.144466645082177E-5	1.8707151436662464E-5	4.944032879689366E-5
14-15	8.485029401629046E-5	1.068980082094998E-5	7.08199304387936E-5	2.472016439844683E-5	6.814748023355611E-5
16-17	1.1224290861997478E-4	1.26941384748781E-5	9.487198228593107E-5	2.6724502052374948E-5	7.816916850319672E-5
18-19	1.2827760985139973E-4	1.5366588680115595E-5	1.1090668351735602E-4	2.8060727154993695E-5	8.150973125974359E-5
20-21	1.423079734288966E-4	1.6034701231424966E-5	1.2493704709485288E-4	3.073317736023119E-5	8.685463167021858E-5
22-23	1.623513499681778E-4	1.6034701231424966E-5	1.423079734288966E-4	3.273751501415931E-5	8.685463167021858E-5
24-25	2.1245979131638082E-4	2.137960164189996E-5	1.4832098639068096E-4	3.674619032201555E-5	8.685463167021858E-5
26-27	2.659087954211307E-4	2.8060727154993695E-5	1.570064495577028E-4	3.9418640527253044E-5	8.819085677283732E-5
28-29	3.4875475178349304E-4	2.8060727154993695E-5	1.7704982609698404E-4	4.142297818118117E-5	9.286764463200295E-5
30-31	4.209109073249054E-4	2.8728839706303067E-5	1.7905416375091213E-4	4.275920328379992E-5	9.62082073885498E-5
32-33	4.903946126610802E-4	3.0065064808921817E-5	1.8306283905876838E-4	4.944032879689366E-5	1.0088499524771542E-4
34-35	5.772492443312988E-4	4.008675307856242E-5	1.9976565284150272E-4	5.3449004104749895E-5	1.089023458634279E-4
36-37	6.955051659130579E-4	4.409542838641866E-5	2.1980902938078395E-4	5.3449004104749895E-5	1.349587353644935E-4
38-39	8.124248623921984E-4	4.8104103694274906E-5	2.3918429336875575E-4	5.411711665605927E-5	1.4832098639068096E-4
40-41	9.533966107184762E-4	5.07765538995124E-5	2.53882769497562E-4	5.678956686129676E-5	1.550021119037747E-4
42-43	0.0010843466707751133	6.280257982308112E-5	2.9263329747350567E-4	5.745767941260613E-5	1.6636002527603403E-4
44-45	0.00122665464420401	6.614314257962799E-5	2.993144229865994E-4	5.9462017066534256E-5	1.9442075243102775E-4
46-47	0.0013950190071339722	6.681125513093737E-5	3.226983622824275E-4	6.013012961784363E-5	1.9976565284150272E-4
48-49	0.001555366019448222	6.681125513093737E-5	3.4073740116778055E-4	6.480691747700924E-5	2.1446412897030895E-4
50-51	0.001725734720032112	6.948370533617487E-5	3.4942286433480246E-4	6.681125513093737E-5	2.411886310226839E-4
52-53	0.0018707151436662462	7.34923806440311E-5	3.734749161819399E-4	6.814748023355611E-5	2.525465443949433E-4
54-55	0.0020397476191475177	7.34923806440311E-5	3.86169054656818E-4	7.148804299010298E-5	2.5989578245934634E-4
56-57	0.002214124995039264	7.34923806440311E-5	4.0420809354217106E-4	7.34923806440311E-5	2.69917470728987E-4
58-59	0.0024112181976755297	7.816916850319672E-5	4.1489789436312106E-4	7.549671829795922E-5	2.8060727154993693E-4
60-61	0.002600962162247392	7.950539360581545E-5	4.563208725443022E-4	7.683294340057797E-5	2.839478343064838E-4
62-63	0.002796719139781038	8.217784381105295E-5	4.977438507254834E-4	7.883728105450609E-5	2.872883970630307E-4
64-65	0.0030131876064052755	8.485029401629046E-5	5.191234523673834E-4	8.418218146498108E-5	2.9864631043529006E-4
66-67	0.0032356690859912966	8.618651911890921E-5	5.211277900213114E-4	8.618651911890921E-5	3.079998861536213E-4
68-69	0.003462159240885174	8.685463167021858E-5	5.358262661501177E-4	8.88589693241467E-5	3.173534618719525E-4
70-71	0.0036719465819963176	8.685463167021858E-5	5.358262661501177E-4	8.952708187545607E-5	3.267070375902837E-4
72-73	0.0038810658105561512	8.819085677283732E-5	5.425073916632114E-4	9.086330697807482E-5	3.4073740116778055E-4
74-75	0.00412826745454062	9.019519442676544E-5	5.518609673815426E-4	9.353575718331231E-5	3.467504141295649E-4
76-77	0.004334046120343907	9.353575718331231E-5	5.57873980343327E-4	9.487198228593106E-5	3.634532279122993E-4
78-79	0.004499738033068632	9.754443249116856E-5	5.638869933051113E-4	9.487198228593106E-5	3.728068036306305E-4
80-81	0.004632692430779197	1.0021688269640604E-4	5.699000062668958E-4	9.620820738854981E-5	3.768154789384867E-4
82-83	0.004722887625205962	1.0021688269640604E-4	5.845984823957019E-4	9.954877014509668E-5	3.888415048620555E-4
84-85	0.004855173910365218	1.0222122035033417E-4	5.95288283216652E-4	1.0689800820949979E-4	3.981950805803867E-4
86-87	0.004909959139572587	1.0422555800426229E-4	5.979607334218894E-4	1.1090668351735602E-4	4.0754865629871794E-4
88-89	0.00491730837763699	1.0957045841473728E-4	5.992969585245082E-4	1.1224290861997478E-4	4.229152449788335E-4
90	0.00491730837763699	1.1491535882521227E-4	5.999650710758176E-4	1.1224290861997478E-4	4.2491958263276165E-4
>>END_MODULE
