##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290303_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89049905
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.083491049204376	37.0	37.0	37.0	37.0	37.0
2	36.16755202602406	37.0	37.0	37.0	37.0	37.0
3	36.284409781234466	37.0	37.0	37.0	37.0	37.0
4	36.315984671741084	37.0	37.0	37.0	37.0	37.0
5	36.33435739207133	37.0	37.0	37.0	37.0	37.0
6	36.30152671134236	37.0	37.0	37.0	37.0	37.0
7	36.26719827494482	37.0	37.0	37.0	37.0	37.0
8	36.328032769939504	37.0	37.0	37.0	37.0	37.0
9	36.30812151905159	37.0	37.0	37.0	37.0	37.0
10-11	36.32695980978306	37.0	37.0	37.0	37.0	37.0
12-13	36.306784937052996	37.0	37.0	37.0	37.0	37.0
14-15	36.30240502783243	37.0	37.0	37.0	37.0	37.0
16-17	36.30281578065693	37.0	37.0	37.0	37.0	37.0
18-19	36.30004448629114	37.0	37.0	37.0	37.0	37.0
20-21	36.2939573658164	37.0	37.0	37.0	37.0	37.0
22-23	36.28048331999905	37.0	37.0	37.0	37.0	37.0
24-25	36.2503992059284	37.0	37.0	37.0	37.0	37.0
26-27	36.20759382056612	37.0	37.0	37.0	37.0	37.0
28-29	36.194237074143985	37.0	37.0	37.0	37.0	37.0
30-31	36.186635729706836	37.0	37.0	37.0	37.0	37.0
32-33	36.18295288467742	37.0	37.0	37.0	37.0	37.0
34-35	36.17027945734473	37.0	37.0	37.0	37.0	37.0
36-37	36.205488368773224	37.0	37.0	37.0	37.0	37.0
38-39	36.202009243907725	37.0	37.0	37.0	37.0	37.0
40-41	36.20203380254996	37.0	37.0	37.0	37.0	37.0
42-43	36.185302633746915	37.0	37.0	37.0	37.0	37.0
44-45	36.18438847052843	37.0	37.0	37.0	37.0	37.0
46-47	36.18192159077589	37.0	37.0	37.0	37.0	37.0
48-49	36.15676258172872	37.0	37.0	37.0	37.0	37.0
50-51	36.15080726917925	37.0	37.0	37.0	37.0	37.0
52-53	36.15439192107615	37.0	37.0	37.0	37.0	37.0
54-55	36.14518162222011	37.0	37.0	37.0	37.0	37.0
56-57	36.131284996937794	37.0	37.0	37.0	37.0	37.0
58-59	36.11840008167053	37.0	37.0	37.0	37.0	37.0
60-61	36.114448390867196	37.0	37.0	37.0	37.0	37.0
62-63	36.10570632767565	37.0	37.0	37.0	37.0	37.0
64-65	36.092154434696205	37.0	37.0	37.0	37.0	37.0
66-67	36.09145790913775	37.0	37.0	37.0	37.0	37.0
68-69	36.073969398312926	37.0	37.0	37.0	37.0	37.0
70-71	36.05711791785815	37.0	37.0	37.0	37.0	37.0
72-73	36.035936299056225	37.0	37.0	37.0	37.0	37.0
74-75	36.03312509457635	37.0	37.0	37.0	37.0	37.0
76-77	36.02238600111407	37.0	37.0	37.0	37.0	37.0
78-79	36.00855301983577	37.0	37.0	37.0	37.0	37.0
80-81	35.98080764753537	37.0	37.0	37.0	37.0	37.0
82-83	35.99253779352316	37.0	37.0	37.0	37.0	37.0
84-85	35.97825458347187	37.0	37.0	37.0	37.0	37.0
86-87	35.992011133617936	37.0	37.0	37.0	37.0	37.0
88-89	35.96848277063351	37.0	37.0	37.0	37.0	37.0
90-91	35.94938286722437	37.0	37.0	37.0	37.0	37.0
92-93	35.939060549294496	37.0	37.0	37.0	37.0	37.0
94-95	35.913769858771325	37.0	37.0	37.0	37.0	37.0
96-97	35.91736251398126	37.0	37.0	37.0	37.0	37.0
98-99	35.898890746025835	37.0	37.0	37.0	37.0	37.0
100-101	35.86804587610889	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125498.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	5.0
15	97.0
16	424.0
17	1166.0
18	1905.0
19	2734.0
20	3981.0
21	6725.0
22	12385.0
23	25515.0
24	49753.0
25	91541.0
26	152632.0
27	252129.0
28	386464.0
29	573365.0
30	785104.0
31	1070520.0
32	1410822.0
33	2019045.0
34	3184488.0
35	7473012.0
36	5.3222865E7
37	1.819773E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.245394357509017	16.006665064875577	12.779053018377617	43.96888755923779
2	22.24954704534828	19.602318985658698	31.40335404515513	26.744779923837893
3	23.926665250008142	20.014990745237355	23.298539957057613	32.75980404769689
4	25.160678327604703	25.132663521725817	20.767616701677866	28.939041448991613
5	26.024273072822705	28.95713123988874	23.011057867121306	22.007537820167254
6	21.547356509220243	33.68529069864925	23.055395803763975	21.711956988366534
7	18.841677516050233	22.036573153644984	38.13554472170953	20.98620460859525
8	20.11808299154584	24.317279956671513	29.107738666168448	26.456898385614195
9	20.890483561745324	22.38447389537747	31.409751140980376	25.31529140189683
10-11	22.787765374558703	30.581865792839817	24.026174546501892	22.604194286099588
12-13	22.152043000531368	25.792069115076693	27.586575109407974	24.469312774983965
14-15	22.14544865566731	26.35184638195017	27.138336317405642	24.36436864497687
16-17	22.951612351379723	26.386218697742652	26.559236457013945	24.102932493863676
18-19	22.751479397405518	26.840037932075933	26.029763682024377	24.378718988494168
20-21	22.734565721653418	26.633597145313786	26.300125602788153	24.33171153024464
22-23	22.63940853047039	26.742128554127444	26.41710664721965	24.20135626818252
24-25	22.653600766868923	26.511197190318477	26.54442346122333	24.29077858158927
26-27	22.821891845062954	26.715370231667656	26.374772272773416	24.087965650495978
28-29	22.66725641781732	26.529940329869277	26.435528413207276	24.367274839106123
30-31	22.694617403690692	26.244039389996388	26.581203407829822	24.4801397984831
32-33	22.882155310430626	26.47625895129889	26.401926907545292	24.239658830725187
34-35	22.82804048015632	26.567246338352962	26.281013638607725	24.323699542882988
36-37	22.58005504933836	26.479868575829958	26.508018173039854	24.432058201791833
38-39	22.96498751989038	26.5838152649894	26.45136654865224	23.99983066646798
40-41	23.06420401255698	26.479788430259354	26.07701583808887	24.378991719094795
42-43	22.837620717696357	26.582054859706165	26.27416765763061	24.306156764966865
44-45	22.999943133136103	26.470186703047062	26.434691027459383	24.09517913635745
46-47	22.932720450468818	26.204368119875234	26.468039265978454	24.3948721636775
48-49	22.72396859480759	26.034128275691476	26.62709793827165	24.614805191229284
50-51	22.97029658980884	26.03126327255254	26.462445762501396	24.535994375137225
52-53	23.152000648071343	26.373280005874218	26.16262869867238	24.31209064738206
54-55	22.726521288909414	26.33688070970639	26.47830404792213	24.458293953462068
56-57	22.833372160390734	26.519949376963663	26.364271071251704	24.2824073913939
58-59	22.864855802345012	26.428830868354357	26.31221927647494	24.394094052825686
60-61	22.88823137150951	26.27291670555757	26.338218547987662	24.500633374945263
62-63	22.99691651484255	26.122064142779568	26.372557552994685	24.508461789383198
64-65	23.03671109246304	26.343204652164932	26.12286414357578	24.497220111796246
66-67	23.08864193015291	26.227155119610522	26.22065448186798	24.463548468368582
68-69	23.019302557170974	26.350359386151347	26.443667114726317	24.186670941951366
70-71	23.395847065312473	26.3126891725237	26.218081359422946	24.073382402740883
72-73	23.170761768257286	26.19746037570606	26.1917450926138	24.440032763422852
74-75	23.264561683778094	26.369085431384555	26.202979107360928	24.163373777476423
76-77	23.22718583737999	26.352817998050377	26.200818437793288	24.219177726776348
78-79	23.01007077454594	26.41959536257345	26.051382747350367	24.518951115530243
80-81	23.28508070648923	26.36273000480494	26.06783421244647	24.284355076259363
82-83	23.30997047601985	26.455656133166805	25.843593789952312	24.39077960086103
84-85	23.16180501361112	26.505883855402146	25.974985451967232	24.357325679019507
86-87	23.164765258396386	26.43421267527315	26.023186888902483	24.37783517742798
88-89	23.314398065639296	26.18471833392602	25.963534906367077	24.537348694067603
90-91	23.17718170345659	26.190478977352644	26.283867609196214	24.348471709994556
92-93	23.5302732895102	26.09673777037821	26.158814933200564	24.214174006911026
94-95	23.34019015480251	26.17871340236672	26.215385401011815	24.265711041818953
96-97	23.33159936820772	25.97091053490011	26.284986471347082	24.41250362554509
98-99	23.52698207101711	25.160113336787145	26.697163757061794	24.615740835133952
100-101	24.40641038375068	11.71097616824125	32.668909804207686	31.213703643800383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125629.0
1	62841.0
2	57.0
3	60.5
4	68.5
5	76.5
6	85.0
7	94.0
8	103.5
9	114.0
10	137.5
11	190.0
12	245.5
13	329.0
14	493.0
15	736.5
16	1069.5
17	1551.0
18	2250.5
19	3345.0
20	4425.0
21	6216.5
22	10568.5
23	16824.5
24	23396.0
25	32367.0
26	41625.5
27	56611.5
28	77815.5
29	101851.0
30	142008.5
31	191750.5
32	277811.5
33	402934.0
34	538158.5
35	697525.0
36	954747.5
37	1317974.5
38	1735816.5
39	2312360.0
40	2892159.0
41	3387028.5
42	3923618.0
43	4504941.0
44	4967531.0
45	5287981.0
46	5445544.5
47	5417914.0
48	5257807.5
49	5045421.5
50	4742318.0
51	4360291.0
52	3861461.5
53	3202257.5
54	2679567.5
55	2332883.0
56	2047041.5
57	1741429.5
58	1453517.5
59	1161626.0
60	950169.0
61	848640.0
62	801582.5
63	741358.0
64	604571.0
65	481750.0
66	422763.0
67	379285.0
68	306984.5
69	228019.0
70	163099.5
71	117662.0
72	91016.0
73	71000.0
74	59215.0
75	43714.0
76	26252.5
77	21478.5
78	16650.0
79	12192.5
80	10220.5
81	6618.0
82	2725.5
83	444.5
84	294.0
85	234.5
86	200.5
87	155.0
88	140.0
89	123.0
90	92.0
91	69.0
92	65.5
93	80.0
94	84.0
95	93.0
96	172.5
97	451.5
98	1371.0
99	1558.0
100	561.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17714448993516613
2	0.15140162137174656
3	0.14266270132461117
4	0.14092996505723393
5	0.14095803920284922
6	0.14092996505723393
7	0.14092996505723393
8	0.14092996505723393
9	0.14093333395470775
10-11	0.14093108802305854
12-13	0.14094961695916464
14-15	0.14100295783583372
16-17	0.14094063323256772
18-19	0.14114782042720875
20-21	0.14096084661741076
22-23	0.14138027435290357
24-25	0.14351334793675524
26-27	0.14528707245673086
28-29	0.14715905648636007
30-31	0.1484583279454369
32-33	0.14973626305384602
34-35	0.15102486633758902
36-37	1.4789510401974393E-4
38-39	3.3740729586930305E-5
40-41	3.092946321095561E-5
42-43	3.4472756343746354E-4
44-45	9.616519358180002E-5
46-47	2.20452344816621E-4
48-49	1.4903263004197845E-4
50-51	7.479865116998336E-5
52-53	1.1473063260873591E-4
54-55	4.780530270252994E-5
56-57	0.0
58-59	1.6873086760692204E-6
60-61	5.624474997442552E-7
62-63	7.311967771005908E-6
64-65	2.8123596298576996E-6
66-67	7.874787680958894E-6
68-69	0.0
70-71	0.0
72-73	9.000445004502135E-6
74-75	0.0
76-77	1.687683843620295E-6
78-79	0.0
80-81	0.0
82-83	5.288643441071383E-5
84-85	5.063805553512685E-6
86-87	9.565398752243248E-6
88-89	2.5602733791265145E-4
90-91	4.445601901605651E-5
92-93	8.442425891681988E-6
94-95	5.921612630290886E-4
96-97	2.5358874280574372E-5
98-99	1.7774377023422895E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	135278.0
36-37	1038.0
38-39	1312.0
40-41	1167.0
42-43	1271.0
44-45	1424.0
46-47	1368.0
48-49	1339.0
50-51	1398.0
52-53	1626.0
54-55	1670.0
56-57	1624.0
58-59	1745.0
60-61	1795.0
62-63	1992.0
64-65	2031.0
66-67	2113.0
68-69	2146.0
70-71	2434.0
72-73	2534.0
74-75	2719.0
76-77	2891.0
78-79	3037.0
80-81	3446.0
82-83	3520.0
84-85	4021.0
86-87	4140.0
88-89	4798.0
90-91	14101.0
92-93	9640.0
94-95	30662.0
96-97	639339.0
98-99	8056175.0
100-101	8.0104111E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.960621175853785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.67143553966323	10.745256207593714
2	16.818535889318714	6.377797754597466
3	7.991550147146563	4.545742648436536
4	4.574668427577396	3.469542202417348
5	2.8770141762869192	2.727498795706864
6	1.9659883942435725	2.236581670762648
7	1.3958650248285873	1.852652756387901
8	1.0608278800622302	1.6091164453315199
9	0.8027186361598887	1.369803957392309
>10	4.960570718256772	17.667543842638047
>50	0.4518930587318166	5.975041890325525
>100	0.3419330280732132	13.031211155870597
>500	0.04373480868262278	5.76012467482574
>1k	0.03856731610349592	14.742529257498157
>5k	0.0036079027413076495	4.552815962667123
>10k+	0.0010890521237650868	3.3367407775485294
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	125498	0.14092996505723393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2459316492252293E-6	0.0	3.368897473837844E-6	1.1229658246126147E-6	5.614829123063073E-6
2	2.2459316492252293E-6	0.0	4.491863298450459E-6	1.1229658246126147E-6	7.860760772288304E-6
3	3.368897473837844E-6	0.0	4.491863298450459E-6	1.1229658246126147E-6	8.983726596900917E-6
4	1.3475589895351376E-5	0.0	1.1229658246126147E-5	1.1229658246126147E-6	1.0106692421513533E-5
5	2.1336350667639678E-5	0.0	1.5721521544576607E-5	1.1229658246126147E-6	1.4598555719963992E-5
6	3.81808380368289E-5	0.0	2.0213384843027066E-5	1.1229658246126147E-6	1.4598555719963992E-5
7	4.042676968605413E-5	0.0	2.358228231686491E-5	1.1229658246126147E-6	1.4598555719963992E-5
8	4.379566715989197E-5	0.0	2.4705248141477524E-5	1.1229658246126147E-6	2.2459316492252293E-5
9	4.491863298450459E-5	0.0	2.6951179790702752E-5	1.1229658246126147E-6	2.358228231686491E-5
10-11	7.804612481057671E-5	0.0	4.267270133527936E-5	1.1229658246126147E-6	2.8635628527621675E-5
12-13	8.42224368459461E-5	0.0	6.288608617830641E-5	1.1229658246126147E-6	3.368897473837844E-5
14-15	9.432912926745964E-5	0.0	7.186981277520734E-5	1.1229658246126147E-6	4.042676968605413E-5
16-17	1.072432362505047E-4	0.0	9.09602317936218E-5	2.8074145615315367E-6	4.2111218422973045E-5
18-19	1.1622696284740563E-4	0.0	9.545209509207226E-5	5.614829123063073E-6	4.4357150072198276E-5
20-21	1.1959586032124345E-4	0.0	1.072432362505047E-4	5.614829123063073E-6	5.109494501987397E-5
22-23	1.3026403565506332E-4	0.0	1.2857958691814438E-4	5.614829123063073E-6	5.502532540601812E-5
24-25	1.471085230242525E-4	5.614829123063073E-7	1.302640356550633E-4	5.614829123063073E-6	5.502532540601812E-5
26-27	1.7181377116573006E-4	2.8074145615315367E-6	1.4093221098888313E-4	5.614829123063073E-6	5.8394222879855966E-5
28-29	2.0550274590410848E-4	3.368897473837844E-6	1.527233521473156E-4	5.614829123063073E-6	6.232460326600011E-5
30-31	2.3133095987019862E-4	3.368897473837844E-6	1.5833818127037867E-4	6.737794947675688E-6	6.513201782753166E-5
32-33	2.453680326778563E-4	3.368897473837844E-6	1.5833818127037867E-4	6.737794947675688E-6	6.85009153013695E-5
34-35	2.6501993460857707E-4	4.491863298450459E-6	1.7967453193801835E-4	8.42224368459461E-6	7.186981277520734E-5
36-37	2.857948023639104E-4	4.491863298450459E-6	1.8753529271030666E-4	1.0106692421513533E-5	7.411574442443257E-5
38-39	3.172378454530637E-4	4.491863298450459E-6	2.0044939969335172E-4	1.0106692421513533E-5	8.815281723209025E-5
40-41	3.3913567903300963E-4	4.491863298450459E-6	2.0831016046564003E-4	1.0106692421513533E-5	9.432912926745964E-5
42-43	3.7001723920985656E-4	5.614829123063073E-6	2.2403168201021664E-4	1.066817533381984E-5	9.769802674129748E-5
44-45	4.0146028229900977E-4	5.614829123063073E-6	2.2683909657174816E-4	1.5721521544576607E-5	9.769802674129748E-5
46-47	4.3683370577430713E-4	5.614829123063073E-6	2.3582282316864908E-4	2.0213384843027066E-5	1.0050544130282902E-4
48-49	4.699611976003793E-4	5.614829123063073E-6	2.4256061811632476E-4	2.0213384843027066E-5	1.0780471916281101E-4
50-51	4.946664457418568E-4	5.614829123063073E-6	2.4817544723938783E-4	2.0213384843027066E-5	1.1734992867201824E-4
52-53	5.233020742694784E-4	5.614829123063073E-6	2.6670438334549596E-4	2.0213384843027066E-5	1.1903437740893715E-4
54-55	5.586754977447757E-4	5.614829123063073E-6	2.762495928547032E-4	2.0213384843027066E-5	1.2128030905816238E-4
56-57	5.962948528692984E-4	5.614829123063073E-6	2.846718365392978E-4	2.0213384843027066E-5	1.319484843919822E-4
58-59	6.48512763713785E-4	5.614829123063073E-6	2.8916369983774824E-4	2.0213384843027066E-5	1.5103890341039666E-4
60-61	6.838861871890824E-4	6.17631203536938E-6	3.1443043089153213E-4	2.0213384843027066E-5	1.532848350596219E-4
62-63	7.220670252259113E-4	6.737794947675688E-6	3.374512302960907E-4	2.0213384843027066E-5	1.5609224962115346E-4
64-65	7.591248974381276E-4	6.737794947675688E-6	3.604720297006493E-4	2.0213384843027066E-5	1.6507597621805435E-4
66-67	8.017975987734069E-4	8.42224368459461E-6	3.627179613498745E-4	2.0213384843027066E-5	1.7012932242881112E-4
68-69	8.321176760379475E-4	1.0106692421513533E-5	3.6889427338524395E-4	2.0213384843027066E-5	1.8079749776263096E-4
70-71	8.753518602855331E-4	1.0106692421513533E-5	3.7507058542061327E-4	2.1336350667639678E-5	1.925886389210634E-4
72-73	9.258853223931008E-4	1.0106692421513533E-5	3.885461753159647E-4	2.1336350667639678E-5	2.0101088260565805E-4
74-75	9.842795452729568E-4	1.0106692421513533E-5	3.952839702636404E-4	2.1336350667639678E-5	2.0494126299180218E-4
76-77	0.0010387433877666686	1.066817533381984E-5	3.96406936088253E-4	2.1336350667639678E-5	2.0943312629025264E-4
78-79	0.001077485708715804	1.2352624070738762E-5	3.986528677374782E-4	2.1336350667639678E-5	2.2347019909791033E-4
80-81	0.001111174683454182	1.3475589895351376E-5	4.0202176521131604E-4	2.1336350667639678E-5	2.296465111332797E-4
82-83	0.0011403717948941102	1.4598555719963992E-5	4.0314473103592865E-4	2.1336350667639678E-5	2.3413837443173017E-4
84-85	0.0011746222525447951	1.7405970281495527E-5	4.0707511142207286E-4	2.2459316492252293E-5	2.3582282316864908E-4
86-87	0.0011858519107909211	2.0213384843027066E-5	4.1605883801897373E-4	2.2459316492252293E-5	2.3975320355479324E-4
88-89	0.0011880978424401464	2.1336350667639678E-5	4.1662032093128006E-4	2.30207994045586E-5	2.436835839409374E-4
90	0.0011880978424401464	2.5828213966090137E-5	4.188662525805053E-4	2.358228231686491E-5	2.459295155901626E-4
>>END_MODULE
