##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290304_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72267237
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.092430100793806	37.0	37.0	37.0	37.0	37.0
2	36.140106961609725	37.0	37.0	37.0	37.0	37.0
3	36.217046640374534	37.0	37.0	37.0	37.0	37.0
4	36.187341699531146	37.0	37.0	37.0	37.0	37.0
5	36.17060897180834	37.0	37.0	37.0	37.0	37.0
6	36.080620835137225	37.0	37.0	37.0	37.0	37.0
7	36.039401603246574	37.0	37.0	37.0	37.0	37.0
8	36.12100721382222	37.0	37.0	37.0	37.0	37.0
9	36.072628153751054	37.0	37.0	37.0	37.0	37.0
10-11	36.10406504125791	37.0	37.0	37.0	37.0	37.0
12-13	36.08446316413065	37.0	37.0	37.0	37.0	37.0
14-15	36.07719462140223	37.0	37.0	37.0	37.0	37.0
16-17	36.05963782730478	37.0	37.0	37.0	37.0	37.0
18-19	36.07012453651715	37.0	37.0	37.0	37.0	37.0
20-21	36.06345643185445	37.0	37.0	37.0	37.0	37.0
22-23	36.03408352252349	37.0	37.0	37.0	37.0	37.0
24-25	36.02316516542621	37.0	37.0	37.0	37.0	37.0
26-27	35.994834498792315	37.0	37.0	37.0	37.0	37.0
28-29	35.98065518265213	37.0	37.0	37.0	37.0	37.0
30-31	35.96229314121972	37.0	37.0	37.0	37.0	37.0
32-33	35.94427592409545	37.0	37.0	37.0	37.0	37.0
34-35	35.92712308898706	37.0	37.0	37.0	37.0	37.0
36-37	35.91477552883771	37.0	37.0	37.0	37.0	37.0
38-39	35.90415951432418	37.0	37.0	37.0	37.0	37.0
40-41	35.909114024394235	37.0	37.0	37.0	37.0	37.0
42-43	35.871249520294384	37.0	37.0	37.0	37.0	37.0
44-45	35.85859034254422	37.0	37.0	37.0	37.0	37.0
46-47	35.86368401258791	37.0	37.0	37.0	37.0	37.0
48-49	35.843437680067126	37.0	37.0	37.0	37.0	37.0
50-51	35.82779318017823	37.0	37.0	37.0	37.0	37.0
52-53	35.816714218302906	37.0	37.0	37.0	37.0	37.0
54-55	35.81305858226497	37.0	37.0	37.0	37.0	37.0
56-57	35.779976693329175	37.0	37.0	37.0	37.0	37.0
58-59	35.80066906669648	37.0	37.0	37.0	37.0	37.0
60-61	35.766976774290335	37.0	37.0	37.0	37.0	37.0
62-63	35.75615541311252	37.0	37.0	37.0	37.0	37.0
64-65	35.743652144640905	37.0	37.0	37.0	37.0	37.0
66-67	35.72588196290681	37.0	37.0	37.0	37.0	37.0
68-69	35.68996579362445	37.0	37.0	37.0	37.0	37.0
70-71	35.701046828573936	37.0	37.0	37.0	37.0	37.0
72-73	35.6785329404845	37.0	37.0	37.0	37.0	37.0
74-75	35.69015581175839	37.0	37.0	37.0	37.0	37.0
76-77	35.66663466576376	37.0	37.0	37.0	37.0	37.0
78-79	35.61864197614308	37.0	37.0	37.0	37.0	37.0
80-81	35.61648134331398	37.0	37.0	37.0	37.0	37.0
82-83	35.58330160927686	37.0	37.0	37.0	37.0	37.0
84-85	35.53921189055444	37.0	37.0	37.0	37.0	37.0
86-87	35.500893811179495	37.0	37.0	37.0	37.0	37.0
88-89	35.52693362592723	37.0	37.0	37.0	37.0	37.0
90-91	35.46393896127458	37.0	37.0	37.0	37.0	37.0
92-93	35.50899084570892	37.0	37.0	37.0	37.0	37.0
94-95	35.46083792699039	37.0	37.0	37.0	37.0	37.0
96-97	35.437753798128035	37.0	37.0	37.0	37.0	37.0
98-99	35.43341526599609	37.0	37.0	37.0	37.0	37.0
100-101	35.40836241100308	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14489.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	33.0
12	919.0
13	7031.0
14	15041.0
15	17059.0
16	15431.0
17	15321.0
18	16265.0
19	19535.0
20	26847.0
21	43304.0
22	65640.0
23	94674.0
24	123769.0
25	157534.0
26	196897.0
27	258237.0
28	335396.0
29	453653.0
30	611107.0
31	875147.0
32	1287260.0
33	2146121.0
34	4032937.0
35	1.0697899E7
36	4.2861107E7
37	7878584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.77179320251829	16.051475828815423	11.762567458500243	46.41416351016605
2	21.969712710259632	19.564153597728417	33.2303269234914	25.235806768520554
3	23.401513357399683	20.270281323894377	23.881313287087636	32.446892031618304
4	24.817447753137483	25.687627315100116	20.99296065598107	28.50196427578134
5	25.930147138863656	29.54388243572781	23.186761279309927	21.339209146098607
6	21.1800817246309	34.09918889472666	23.34887904370138	21.371850336941062
7	18.701913847169173	21.757087990809595	38.74421286644926	20.79678529557198
8	19.785930327366458	23.95364015616893	29.765166827766564	26.495262688698045
9	20.845769077184443	22.066633368740522	31.93245328191531	25.155144272159724
10-11	22.786472006044672	30.62109645334751	24.16367961661459	22.428751923993232
12-13	22.050753277036918	25.804117846973913	27.864468269563808	24.28066060642536
14-15	22.137624656871125	26.402924727999228	27.263531584464847	24.195919030664804
16-17	23.00721559190782	26.432373371996455	26.649800823909093	23.91061021218664
18-19	22.721525128323748	26.905323075087	26.130098071559278	24.243053725029974
20-21	22.69980209876427	26.720097144595567	26.431456056033998	24.148644700606166
22-23	22.541832300975685	26.851596480256372	26.640119272619167	23.96645194614878
24-25	22.605584656980405	26.5769781170217	26.73553555979216	24.081901666205734
26-27	22.755839821966735	26.84509324599996	26.532569761668785	23.866497170364525
28-29	22.593650823859974	26.61879630887201	26.622251788771422	24.165301078496594
30-31	22.635630182304673	26.323942421401075	26.734332468355877	24.306094927938375
32-33	22.826384705590534	26.59491402583654	26.537241490617475	24.041459777955446
34-35	22.78400887812759	26.681327110316694	26.406554354598722	24.128109656956997
36-37	22.50912441488983	26.591687260263953	26.607813362117067	24.291374962729154
38-39	22.893709976790177	26.735871676122496	26.632687692637695	23.737730654449628
40-41	23.0064127607767	26.62611604823904	26.206300253238858	24.1611709377454
42-43	22.76144846362629	26.717605321785847	26.38456215264989	24.13638406193797
44-45	22.936964358346327	26.58026270810165	26.60045571095968	23.88231722259234
46-47	22.830156787369578	26.33691693059534	26.640467721495376	24.192458560539713
48-49	22.61049701729597	26.1706896674884	26.77167217760015	24.447141137615482
50-51	22.87786392516975	26.16677574009911	26.616055929509635	24.339304405221508
52-53	23.101102590571948	26.494032936079144	26.272332958959126	24.132531514389786
54-55	22.6125335696264	26.486878764198885	26.62347963692438	24.277108029250336
56-57	22.752828699677103	26.686320550233056	26.492360483517796	24.06849026657205
58-59	22.76805563602827	26.589265418104013	26.447811353415307	24.194867592452415
60-61	22.81468304489741	26.428212332720495	26.44496898534384	24.31213563703825
62-63	22.94798505456559	26.250834320136807	26.54292113285002	24.25825949244759
64-65	22.957738763336035	26.478892469289363	26.273002808084733	24.29036595928987
66-67	23.02496280070658	26.38440936926231	26.35255116136868	24.238076668662426
68-69	22.99459064225966	26.495588659876883	26.559361290879067	23.950459406984386
70-71	23.359696068183354	26.472040097580685	26.306078269396576	23.862185564839383
72-73	23.07906989856998	26.34069954511965	26.32092876103788	24.259301795272492
74-75	23.205358338432642	26.51144273812041	26.338584132778937	23.94461479066801
76-77	23.155123224382464	26.53303951556516	26.316580585771064	23.99525667428131
78-79	22.94575473208686	26.5832150892236	26.178738009770306	24.292292168919232
80-81	23.180402748028506	26.49295462006031	26.237615031453043	24.089027600458138
82-83	23.219409585168886	26.604613784592978	25.94508661049733	24.230890019740805
84-85	23.095793313495424	26.643654597671834	26.073259420540495	24.187292668292244
86-87	23.06324510889199	26.60134201163405	26.1476579838922	24.187754895581758
88-89	23.21190431960812	26.35376369149886	26.09787284758362	24.3364591413094
90-91	23.113187908955112	26.35650853026266	26.41196265368243	24.118340907099803
92-93	23.490876759974327	26.243799241268405	26.29692153642975	23.968402462327518
94-95	23.29001098810907	26.332890080383336	26.350191778032922	24.02690715347467
96-97	23.313759387362616	26.09197003085414	26.394924472695518	24.19934610908772
98-99	23.497572809161035	25.29507122762827	26.817062360655086	24.390293602555612
100-101	24.406990936196397	11.789557428564363	32.768655628212414	31.03479600702682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14597.0
1	7374.0
2	259.0
3	510.0
4	836.5
5	1233.0
6	1617.5
7	1868.5
8	2130.5
9	2385.0
10	2633.0
11	2996.5
12	3233.5
13	3429.5
14	3699.0
15	4060.0
16	4489.0
17	4942.5
18	5700.0
19	6611.5
20	7595.0
21	9280.5
22	12647.5
23	17466.0
24	22655.5
25	29334.0
26	37545.0
27	51548.5
28	69186.5
29	89411.5
30	122170.5
31	163568.0
32	235439.0
33	339462.5
34	455359.5
35	592040.5
36	803778.5
37	1103610.0
38	1461568.5
39	1961144.5
40	2461519.5
41	2881156.0
42	3319288.0
43	3777854.5
44	4145323.5
45	4378474.5
46	4471923.0
47	4430954.0
48	4270011.0
49	4073431.0
50	3806850.0
51	3494057.5
52	3100071.5
53	2546471.0
54	2099522.0
55	1810528.0
56	1576733.0
57	1336805.0
58	1114543.5
59	882001.5
60	712068.5
61	632953.0
62	595958.5
63	549550.0
64	450803.0
65	357816.5
66	308644.0
67	275457.0
68	223634.5
69	167722.0
70	118952.0
71	84662.0
72	64733.0
73	50411.0
74	41550.0
75	30286.5
76	18538.5
77	14272.0
78	11174.0
79	8907.5
80	7496.5
81	5233.5
82	2993.5
83	1663.0
84	1458.0
85	1320.0
86	1229.0
87	1149.0
88	1054.0
89	967.0
90	896.5
91	848.5
92	787.5
93	731.0
94	744.5
95	742.0
96	755.5
97	831.5
98	968.0
99	1375.5
100	7493.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06644919882574174
2	0.0356703826936126
3	0.020808876365371487
4	0.02062898848616559
5	0.020587475898656536
6	0.021558870446368388
7	0.020061649790208528
8	0.02070924615534976
9	0.020049196013955814
10-11	0.020083789836880025
12-13	0.020069260431251856
14-15	0.020065801048959435
16-17	0.020882215269970816
18-19	0.020114924277511815
20-21	0.020105929883551518
22-23	0.02207362653148065
24-25	0.02319930952943448
26-27	0.02527632265780412
28-29	0.02722464676489569
30-31	0.028845021430665738
32-33	0.030825863731306066
34-35	0.03206847385074373
36-37	2.0901599057456923E-4
38-39	5.481558514502073E-4
40-41	6.900514370708769E-4
42-43	0.0011406453429433628
44-45	9.233295773984203E-4
46-47	6.146396448745027E-4
48-49	5.786571261888116E-4
50-51	0.0010355096899826578
52-53	3.7793991569364904E-4
54-55	2.4919581395887796E-5
56-57	9.552696178235534E-5
58-59	2.0767146706185164E-5
60-61	4.4304110252218245E-5
62-63	3.461334666141015E-6
64-65	9.691954047815185E-6
66-67	9.692182782438202E-6
68-69	1.5923251147941792E-5
70-71	2.007764273686047E-5
72-73	0.0
74-75	3.4618524116077437E-6
76-77	3.3927177805449586E-5
78-79	6.231721797382282E-5
80-81	2.7766795313945446E-4
82-83	0.001553197617376851
84-85	1.6412070555754465E-4
86-87	0.001304711508548582
88-89	5.512724097861574E-4
90-91	0.0022668735595691154
92-93	0.0010369847719167234
94-95	0.001088358740332624
96-97	0.0018622878307958985
98-99	0.0016671210430243286
100-101	3.4710362047097134E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	23731.0
36-37	1002.0
38-39	1225.0
40-41	1135.0
42-43	1297.0
44-45	1117.0
46-47	1187.0
48-49	1264.0
50-51	1271.0
52-53	1311.0
54-55	1418.0
56-57	1468.0
58-59	1370.0
60-61	1543.0
62-63	1620.0
64-65	1665.0
66-67	1747.0
68-69	1752.0
70-71	1955.0
72-73	1978.0
74-75	2137.0
76-77	2237.0
78-79	2580.0
80-81	2672.0
82-83	2844.0
84-85	3065.0
86-87	3149.0
88-89	3919.0
90-91	10885.0
92-93	7383.0
94-95	24861.0
96-97	527116.0
98-99	6553004.0
100-101	6.5070329E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.847386244872585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.27113778062438	14.455801230264754
2	14.935912211148997	6.8249311041526015
3	6.86282669166934	4.703929564685715
4	3.8225168382509342	3.493380745241929
5	2.360159257732875	2.6961735080417384
6	1.5772887976351482	2.1622155827568545
7	1.1048995053150092	1.7670846031790466
8	0.846267000154658	1.5467991215058494
9	0.647150223499271	1.3307122003269116
>10	3.8769888107088923	16.6307739785449
>50	0.3594242268223775	5.731421991003236
>100	0.2664593783617938	12.290976696044527
>500	0.03494876434439338	5.548357544195094
>1k	0.030567310262775753	13.979292810353336
>5k	0.0026410864475751145	4.004453673849986
>10k+	8.121170213473868E-4	2.833695645853738
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7675058339368918E-6	0.0	0.0	0.0	1.3837529169684459E-6
2	4.151258750905338E-6	2.7675058339368918E-6	1.3837529169684459E-6	0.0	1.3837529169684459E-6
3	4.151258750905338E-6	2.7675058339368918E-6	1.3837529169684459E-6	0.0	2.7675058339368918E-6
4	1.1070023335747567E-5	2.7675058339368918E-6	2.7675058339368918E-6	1.3837529169684459E-6	8.302517501810675E-6
5	2.0756293754526687E-5	2.7675058339368918E-6	4.151258750905338E-6	1.3837529169684459E-6	1.1070023335747567E-5
6	2.2140046671495134E-5	2.7675058339368918E-6	1.1070023335747567E-5	1.3837529169684459E-6	1.1070023335747567E-5
7	2.2140046671495134E-5	2.7675058339368918E-6	1.3837529169684459E-5	1.3837529169684459E-6	1.1070023335747567E-5
8	2.4907552505432024E-5	2.7675058339368918E-6	1.3837529169684459E-5	1.3837529169684459E-6	1.2453776252716012E-5
9	2.9058811256337364E-5	2.7675058339368918E-6	1.3837529169684459E-5	1.3837529169684459E-6	1.2453776252716012E-5
10-11	4.5663846259958715E-5	2.7675058339368918E-6	1.729691146210557E-5	1.3837529169684459E-6	1.660503500362135E-5
12-13	5.8809498971158945E-5	2.7675058339368918E-6	2.0756293754526687E-5	1.3837529169684459E-6	2.0756293754526687E-5
14-15	7.195515168235918E-5	2.7675058339368918E-6	2.905881125633736E-5	2.7675058339368918E-6	2.4215676046947804E-5
16-17	9.132769251991743E-5	2.7675058339368918E-6	4.22044639675376E-5	3.4593822924211147E-6	2.629130542240047E-5
18-19	9.824645710475966E-5	2.7675058339368918E-6	5.465824022025361E-5	4.151258750905338E-6	3.113444063179003E-5
20-21	1.08624603982023E-4	2.7675058339368918E-6	6.296075772206429E-5	5.5350116678737835E-6	3.666945229966382E-5
22-23	1.2107838023473901E-4	4.151258750905338E-6	7.264702814084341E-5	5.5350116678737835E-6	3.736132875814804E-5
24-25	1.4252655044774993E-4	4.151258750905338E-6	7.333890459932764E-5	5.5350116678737835E-6	3.736132875814804E-5
26-27	1.722772381625715E-4	4.151258750905338E-6	7.403078105781186E-5	6.9187645848422294E-6	3.736132875814804E-5
28-29	2.1378982567162488E-4	4.151258750905338E-6	8.302517501810675E-5	8.302517501810675E-6	4.843135209389561E-5
30-31	2.5806991901461515E-4	6.9187645848422294E-6	8.579268085204364E-5	8.302517501810675E-6	5.3274487303285165E-5
32-33	2.88512483187921E-4	6.9187645848422294E-6	8.579268085204364E-5	9.68627041877912E-6	5.811762251267472E-5
34-35	3.237981825706163E-4	6.9187645848422294E-6	1.0101396293869654E-4	1.0378146877263344E-5	6.711201647296963E-5
36-37	3.860670638341964E-4	6.9187645848422294E-6	1.1070023335747567E-4	1.1070023335747567E-5	7.403078105781186E-5
38-39	4.6217347426746094E-4	7.610641043326452E-6	1.1208398627444412E-4	1.1070023335747567E-5	8.302517501810675E-5
40-41	5.258261084480094E-4	8.302517501810675E-6	1.3422403294593925E-4	1.1070023335747567E-5	9.478707481233855E-5
42-43	5.977812601303687E-4	8.302517501810675E-6	1.4391030336471838E-4	1.2453776252716014E-5	9.686270418779122E-5
44-45	6.621257707694014E-4	8.994393960294897E-6	1.4391030336471838E-4	1.5221282086652904E-5	9.686270418779122E-5
46-47	7.596803514156768E-4	9.68627041877912E-6	1.5913158545137127E-4	1.5221282086652904E-5	9.755458064627544E-5
48-49	8.593105614374049E-4	9.68627041877912E-6	1.632828442022766E-4	1.5221282086652904E-5	1.0378146877263344E-4
50-51	9.333413424952167E-4	1.2453776252716012E-5	1.6674222649469772E-4	1.5221282086652904E-5	1.0793272752353878E-4
52-53	9.9284271792486E-4	1.2453776252716012E-5	1.7642849691347685E-4	1.5221282086652904E-5	1.1415961564989679E-4
54-55	0.0010814029046108404	1.3145652711200235E-5	1.805797556643822E-4	1.5221282086652904E-5	1.238458860686759E-4
56-57	0.0011844924969249895	1.5221282086652904E-5	1.8749852024922442E-4	1.660503500362135E-5	1.3145652711200236E-4
58-59	0.0012758201894449072	1.5221282086652904E-5	1.9372540837558243E-4	1.660503500362135E-5	1.473696856571395E-4
60-61	0.0013740666465496668	1.5221282086652904E-5	2.0617918462829845E-4	1.660503500362135E-5	1.508290679495606E-4
62-63	0.0014916856444919845	1.5221282086652904E-5	2.3108673713373047E-4	1.7988787920589797E-5	1.5290469732501328E-4
64-65	0.001616915283477629	1.5913158545137127E-5	2.504592779712887E-4	1.7988787920589797E-5	1.6259096774379238E-4
66-67	0.0017338424049614626	1.660503500362135E-5	2.539186602637098E-4	1.7988787920589797E-5	1.6535847357772928E-4
68-69	0.001868758314365886	1.660503500362135E-5	2.6152930130703627E-4	1.7988787920589797E-5	1.7435286753802418E-4
70-71	0.0019808423006403304	1.660503500362135E-5	2.622211777655205E-4	1.7988787920589797E-5	1.895741496246771E-4
72-73	0.0020908506575393216	1.937254083755824E-5	2.649886835994574E-4	1.7988787920589797E-5	1.9787666712648777E-4
74-75	0.0022416797254888824	1.937254083755824E-5	2.691399423503627E-4	1.937254083755824E-5	2.0341167879436153E-4
76-77	0.0023828225230196638	2.0064417296042464E-5	2.6983181880884695E-4	1.937254083755824E-5	2.0410355525284576E-4
78-79	0.0024589289334529287	2.2140046671495134E-5	2.6983181880884695E-4	1.937254083755824E-5	2.0756293754526687E-4
80-81	0.002528808455759835	2.352379958846358E-5	2.705236952673312E-4	1.937254083755824E-5	2.0894669046223532E-4
82-83	0.002573088549102825	2.352379958846358E-5	2.7536683047672074E-4	1.937254083755824E-5	2.2070859025646712E-4
84-85	0.0026353574303664053	2.352379958846358E-5	2.795180892276261E-4	1.937254083755824E-5	2.2831923129979358E-4
86-87	0.0026616487357888057	2.352379958846358E-5	2.829774715200472E-4	1.937254083755824E-5	2.3592987234312003E-4
88-89	0.002669259376832132	2.4907552505432024E-5	2.864368538124683E-4	2.0064417296042464E-5	2.449242663034149E-4
90	0.002669259376832132	2.4907552505432024E-5	2.9058811256337365E-4	2.0756293754526687E-5	2.4630801922038336E-4
>>END_MODULE
