##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290294_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89782506
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.39644986073345	37.0	37.0	37.0	37.0	37.0
2	36.40979879755194	37.0	37.0	37.0	37.0	37.0
3	36.47045758557909	37.0	37.0	37.0	37.0	37.0
4	36.44017382406323	37.0	37.0	37.0	37.0	37.0
5	36.41550812805337	37.0	37.0	37.0	37.0	37.0
6	36.34835095825906	37.0	37.0	37.0	37.0	37.0
7	36.32725566827016	37.0	37.0	37.0	37.0	37.0
8	36.38834352652175	37.0	37.0	37.0	37.0	37.0
9	36.34785875769607	37.0	37.0	37.0	37.0	37.0
10-11	36.36653128171762	37.0	37.0	37.0	37.0	37.0
12-13	36.35577239289801	37.0	37.0	37.0	37.0	37.0
14-15	36.34514156354691	37.0	37.0	37.0	37.0	37.0
16-17	36.33051207659541	37.0	37.0	37.0	37.0	37.0
18-19	36.333974126318104	37.0	37.0	37.0	37.0	37.0
20-21	36.32845534518718	37.0	37.0	37.0	37.0	37.0
22-23	36.30025222285508	37.0	37.0	37.0	37.0	37.0
24-25	36.28571593891576	37.0	37.0	37.0	37.0	37.0
26-27	36.26174268292311	37.0	37.0	37.0	37.0	37.0
28-29	36.24972946288668	37.0	37.0	37.0	37.0	37.0
30-31	36.23232563813713	37.0	37.0	37.0	37.0	37.0
32-33	36.212447573027205	37.0	37.0	37.0	37.0	37.0
34-35	36.19958334087934	37.0	37.0	37.0	37.0	37.0
36-37	36.18443578674315	37.0	37.0	37.0	37.0	37.0
38-39	36.17241914289919	37.0	37.0	37.0	37.0	37.0
40-41	36.172355162962745	37.0	37.0	37.0	37.0	37.0
42-43	36.143943683934154	37.0	37.0	37.0	37.0	37.0
44-45	36.134886086666896	37.0	37.0	37.0	37.0	37.0
46-47	36.13367029922085	37.0	37.0	37.0	37.0	37.0
48-49	36.11267971717667	37.0	37.0	37.0	37.0	37.0
50-51	36.09652217768865	37.0	37.0	37.0	37.0	37.0
52-53	36.085875481856405	37.0	37.0	37.0	37.0	37.0
54-55	36.07980483069567	37.0	37.0	37.0	37.0	37.0
56-57	36.05258193740882	37.0	37.0	37.0	37.0	37.0
58-59	36.06985142423976	37.0	37.0	37.0	37.0	37.0
60-61	36.04231742215353	37.0	37.0	37.0	37.0	37.0
62-63	36.03180456301523	37.0	37.0	37.0	37.0	37.0
64-65	36.021926762266034	37.0	37.0	37.0	37.0	37.0
66-67	36.00455504122624	37.0	37.0	37.0	37.0	37.0
68-69	35.978973398652656	37.0	37.0	37.0	37.0	37.0
70-71	35.980467348066995	37.0	37.0	37.0	37.0	37.0
72-73	35.96060570602159	37.0	37.0	37.0	37.0	37.0
74-75	35.96869824083504	37.0	37.0	37.0	37.0	37.0
76-77	35.94844316717657	37.0	37.0	37.0	37.0	37.0
78-79	35.911397489935	37.0	37.0	37.0	37.0	37.0
80-81	35.90979024606219	37.0	37.0	37.0	37.0	37.0
82-83	35.883505095711115	37.0	37.0	37.0	37.0	37.0
84-85	35.8443670228813	37.0	37.0	37.0	37.0	37.0
86-87	35.813541797984534	37.0	37.0	37.0	37.0	37.0
88-89	35.832290819256286	37.0	37.0	37.0	37.0	37.0
90-91	35.78431798982875	37.0	37.0	37.0	37.0	37.0
92-93	35.81509294334208	37.0	37.0	37.0	37.0	37.0
94-95	35.77216731126978	37.0	37.0	37.0	37.0	37.0
96-97	35.74916782628013	37.0	37.0	37.0	37.0	37.0
98-99	35.74449261430239	37.0	37.0	37.0	37.0	37.0
100-101	35.70950560502545	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6982.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	32.0
12	678.0
13	4950.0
14	11751.0
15	17293.0
16	22013.0
17	28209.0
18	32756.0
19	39114.0
20	45965.0
21	59821.0
22	77309.0
23	100929.0
24	123986.0
25	148822.0
26	174873.0
27	221102.0
28	280065.0
29	374220.0
30	500154.0
31	717085.0
32	1048695.0
33	1758696.0
34	3366475.0
35	9623357.0
36	5.4707301E7
37	1.6289873E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.297915877795468	16.593614101160018	17.166192781164614	45.9422772398799
2	23.266513093962658	21.3958312957505	30.13789580767195	25.199759802614885
3	25.2154337014376	21.828286289804932	22.094211590586603	30.862068418170864
4	24.753843685623412	25.560450498411214	20.69623038680705	28.98947542915833
5	26.61802507078389	29.31634709278523	23.398376485929855	20.66725135050103
6	21.893775315479548	34.01229846586975	22.383245978540543	21.710680240110168
7	19.403169331896	21.358166771398267	38.84736989642855	20.39129400027718
8	20.77246697470305	24.375967788677844	28.56599561750522	26.285569619113886
9	21.199790085317353	22.84694064877663	31.0410362308006	24.912233035105416
10-11	23.261955980679268	30.586619033471585	23.37141514619224	22.780009839656902
12-13	22.34758121817666	25.88152585191365	27.29117824694735	24.479714682962335
14-15	22.530803543837425	26.37872446868345	26.85338790040689	24.237084087072237
16-17	23.204019015038558	26.505425677542398	26.194455104614207	24.096100202804838
18-19	22.980477672172825	26.97804328759678	25.928646293126288	24.1128327471041
20-21	23.20794992725351	26.9619673250808	26.058659814430584	23.771422933235108
22-23	22.923907026676307	26.963490729595257	26.363597994545547	23.749004249182885
24-25	22.719127395990817	26.954009902038933	26.48085021896816	23.84601248300208
26-27	23.041061588785226	26.94858566621351	26.27727074996871	23.733081995032553
28-29	22.975176641347055	26.90170520305261	26.13910248484221	23.98401567075813
30-31	22.809441373591753	26.99631782103372	26.258741908410776	23.935498896963754
32-33	23.139551547140606	26.973542426112513	26.131766151750263	23.755139874996622
34-35	23.116949344778327	26.775644392875886	26.1479526255807	23.959453636765087
36-37	22.943870973861678	26.760158963573605	26.27780068043363	24.01816938213109
38-39	23.32246188477443	26.924972088360555	26.034383570507842	23.718182456357173
40-41	23.2929321813026	26.77246967479444	26.00080798293557	23.933790160967384
42-43	23.067758789384204	26.74985565769369	26.329330087041647	23.85305546588046
44-45	23.226968139238043	26.73718024899634	26.302417729891083	23.733433881874532
46-47	23.190087367439975	26.615662498039484	26.22759338100783	23.966656753512712
48-49	23.019697707482674	26.64479608967827	26.22482668748879	24.110679515350263
50-51	23.339363076472434	26.7978490885058	26.000401302012733	23.862386533009033
52-53	23.4469175221238	26.76391085582644	25.970876122902386	23.818295499147375
54-55	23.074318317302296	26.684618814131987	26.323848871871657	23.917213996694056
56-57	23.271586483134865	26.80046542104429	26.214919150628603	23.713028945192246
58-59	23.350028263900544	26.590393941941027	26.16628584512784	23.89329194903059
60-61	23.235457018028825	26.585265974602834	26.165913132641393	24.01336387472695
62-63	23.36645011692144	26.65657501795654	26.22592671306558	23.75104815205644
64-65	23.403184456550306	26.664351510761815	26.14029716627982	23.792166866408067
66-67	23.32877863924851	26.591432673474408	26.29207339130501	23.78771529597207
68-69	23.59027847868993	26.674862472119052	26.06995032964964	23.66490871954138
70-71	23.608783231066806	26.68788171818332	25.94939334906541	23.753941701684465
72-73	23.30067743007114	26.800338277226615	26.12078966778895	23.778194624913297
74-75	23.40106025370709	26.76011398244924	26.235405897936264	23.6034198659074
76-77	23.450690195060666	26.57864128033752	26.198454916086177	23.77221360851563
78-79	23.31308261514944	26.631129209969735	26.26457290111061	23.79121527377022
80-81	23.5879194562673	26.709914668467356	26.062896813668036	23.63926906159731
82-83	23.564947903827935	26.718354974763713	25.990554757571616	23.726142363836733
84-85	23.396401538590116	26.761393939537687	26.130056878834147	23.712147643038055
86-87	23.56673659316022	26.647327878735926	26.200935587036795	23.584999941067064
88-89	23.695219662773557	26.459307021585012	26.11439628034338	23.73107703529805
90-91	23.48343602525426	26.555540775396224	26.226090800354797	23.734932398994715
92-93	23.61892049093205	26.65480317873053	26.131579618627466	23.594696711709954
94-95	23.632453091948914	26.55591211544278	26.14441334060183	23.66722145200647
96-97	23.517680985012664	26.433942238810044	26.314594953655877	23.73378182252141
98-99	23.790432320144422	25.535800101166846	26.716860392023	23.95690718666573
100-101	24.54121402731758	12.017572233753219	33.07441285265028	30.366800886278916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7737.0
1	4054.5
2	417.0
3	676.5
4	1108.5
5	1487.0
6	1712.0
7	1861.5
8	2052.0
9	2210.0
10	2344.0
11	2440.5
12	2602.5
13	2862.5
14	3103.0
15	3530.0
16	4073.5
17	4647.5
18	5444.0
19	6686.5
20	8317.0
21	10404.0
22	13731.0
23	18883.0
24	26077.5
25	35129.0
26	47750.5
27	67692.5
28	96069.0
29	130229.5
30	180234.0
31	249205.5
32	345536.0
33	469731.0
34	627965.5
35	840106.0
36	1122777.0
37	1476107.0
38	1918463.5
39	2439480.0
40	2986131.5
41	3547455.0
42	4106682.5
43	4593064.5
44	4983309.0
45	5259434.5
46	5404590.0
47	5426413.5
48	5247176.5
49	4916591.5
50	4526795.5
51	4106878.5
52	3695904.0
53	3270656.0
54	2828162.5
55	2436379.5
56	2095203.0
57	1741453.5
58	1394392.0
59	1126243.0
60	950095.5
61	820056.0
62	712721.0
63	620891.0
64	538940.0
65	470759.5
66	415881.0
67	364703.5
68	295493.0
69	219859.0
70	167808.0
71	132308.5
72	99980.5
73	76513.0
74	61783.5
75	47263.5
76	30968.5
77	19512.5
78	13780.0
79	10168.5
80	8547.0
81	7116.5
82	5239.0
83	3976.5
84	3707.0
85	3519.0
86	3301.5
87	3162.0
88	3031.0
89	2881.0
90	2808.5
91	2803.5
92	2826.0
93	2814.5
94	2901.0
95	2977.0
96	2686.0
97	2222.0
98	1838.5
99	1819.5
100	8594.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05553420395728317
2	0.02375629835950447
3	0.00854398071713436
4	0.008396958757199315
5	0.008343496226313843
6	0.009335894455875402
7	0.007787708665650299
8	0.008479380158981083
9	0.007779912046562835
10-11	0.007816667536546596
12-13	0.007802188101098448
14-15	0.007796062186101155
16-17	0.008658145496629377
18-19	0.007845626407442893
20-21	0.007844512604716112
22-23	0.009511875286706745
24-25	0.009208920945022408
26-27	0.010262578324556902
28-29	0.011367470629523306
30-31	0.012479045750850393
32-33	0.01413582730693661
34-35	0.015330937632772247
36-37	2.133281620655941E-4
38-39	5.725974436498529E-4
40-41	6.890221739868329E-4
42-43	0.0011613870004343254
44-45	9.358101368235189E-4
46-47	6.612067846010388E-4
48-49	6.445085840967502E-4
50-51	0.001070676879304896
52-53	3.8828387446317733E-4
54-55	3.7325317652659126E-5
56-57	9.916552599849058E-5
58-59	2.395646555310404E-5
60-61	5.738625988967921E-5
62-63	2.785860161709933E-6
64-65	1.8387516440947087E-5
66-67	1.2816204961118392E-5
68-69	2.7863107926622887E-5
70-71	2.8422660703005422E-5
72-73	0.0
74-75	9.476295114662168E-6
76-77	3.400781933427375E-5
78-79	6.189357953253597E-5
80-81	2.872267925241302E-4
82-83	0.0016847989433744375
84-85	1.9142348238626036E-4
86-87	0.0013707094018013122
88-89	5.910275427214315E-4
90-91	0.0023488945041492087
92-93	0.001042347390942376
94-95	0.0011374635319306762
96-97	0.0019340346141347544
98-99	0.0017754577801604146
100-101	3.7584308741912896E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	14381.0
36-37	1356.0
38-39	1507.0
40-41	1508.0
42-43	1572.0
44-45	1648.0
46-47	1748.0
48-49	1894.0
50-51	2299.0
52-53	2572.0
54-55	2395.0
56-57	2756.0
58-59	3213.0
60-61	3752.0
62-63	4027.0
64-65	4459.0
66-67	5387.0
68-69	6862.0
70-71	8640.0
72-73	10167.0
74-75	11715.0
76-77	14319.0
78-79	18305.0
80-81	23980.0
82-83	30895.0
84-85	38257.0
86-87	44718.0
88-89	54893.0
90-91	76935.0
92-93	83618.0
94-95	119799.0
96-97	746248.0
98-99	8411137.0
100-101	8.0025544E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.241010940425028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.89774965354725	11.364331346602558
2	17.13753813927284	7.965874227735823
3	9.89484681595585	6.898987293103791
4	6.070917811166748	5.643770690709901
5	4.049330778895153	4.705527046685101
6	2.7731005775713102	3.8669796517340274
7	1.9303993530357837	3.140510273830583
8	1.3754080003137619	2.55726979062722
9	1.0777985436527306	2.2544214970146546
>10	6.077928955623301	25.70146090640198
>50	0.42900521634200345	6.866832648928714
>100	0.2559149590140458	11.193315507573805
>500	0.020228019536805877	3.2157740677101017
>1k	0.009470833553691767	3.883877568580781
>5k	2.6609528721125905E-4	0.4086061472887082
>10k+	9.624723154449796E-5	0.33246133547216494
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2276054535612985E-6	0.0	2.2276054535612985E-6	0.0	2.5617462715954933E-5
2	2.2276054535612985E-6	0.0	7.796619087464545E-6	0.0	4.9007319978348566E-5
3	1.1138027267806493E-5	0.0	7.796619087464545E-6	0.0	6.125914997293571E-5
4	2.895887089629688E-5	0.0	1.7820843628490388E-5	0.0	7.685238814786479E-5
5	3.898309543732272E-5	0.0	2.5617462715954933E-5	3.3414081803419475E-6	8.130759905498739E-5
6	6.237295269971636E-5	0.0	4.455210907122597E-5	3.3414081803419475E-6	8.130759905498739E-5
7	6.3486755426497E-5	1.0024224541025843E-5	7.128337451396155E-5	6.682816360683895E-6	8.242140178176804E-5
8	7.128337451396155E-5	1.0024224541025843E-5	7.907999360142609E-5	6.682816360683895E-6	8.353520450854869E-5
9	7.796619087464544E-5	1.0024224541025843E-5	8.242140178176804E-5	6.682816360683895E-6	9.467323177635518E-5
10-11	8.910421814245194E-5	1.447943544814844E-5	1.0246985086381972E-4	6.682816360683895E-6	1.1249407540484557E-4
12-13	1.0191294950042941E-4	1.6707040901709738E-5	1.225182999458714E-4	6.682816360683895E-6	1.4869266402521667E-4
14-15	1.2251829994587143E-4	1.6707040901709738E-5	1.5648928311268122E-4	7.796619087464545E-6	1.6372900083675543E-4
16-17	1.3755463675741016E-4	1.8934646355271035E-5	1.965861812767846E-4	7.796619087464545E-6	1.6929801447065868E-4
18-19	1.5481857902251026E-4	2.0048449082051685E-5	2.0883801127137173E-4	7.796619087464545E-6	1.720825212876103E-4
20-21	1.6929801447065868E-4	2.1162251808832336E-5	2.3612617807749765E-4	7.796619087464545E-6	1.7263942265100064E-4
22-23	1.826636471920265E-4	2.2276054535612986E-5	2.628574435202332E-4	7.796619087464545E-6	1.815498444652458E-4
24-25	2.0493970172763944E-4	2.3389857262393633E-5	2.695402598809171E-4	7.796619087464545E-6	1.8266364719202646E-4
26-27	2.3167096717037505E-4	2.3389857262393633E-5	2.812351885121139E-4	7.796619087464545E-6	1.837774499188071E-4
28-29	2.695402598809171E-4	2.3389857262393633E-5	2.918163144165301E-4	7.796619087464545E-6	1.876757594625394E-4
30-31	3.1687687576909473E-4	2.3389857262393633E-5	2.979422294138237E-4	1.0024224541025843E-5	1.9825688536695557E-4
32-33	3.547461684796368E-4	2.4503659989174283E-5	3.1631997440570436E-4	1.1138027267806493E-5	2.0382589900085882E-4
34-35	4.004120802776434E-4	3.174337771324851E-5	3.731239134715175E-4	1.336563272136779E-5	2.1997603853917823E-4
36-37	4.66683342521092E-4	3.508478589359045E-5	3.9205855982678853E-4	1.336563272136779E-5	2.4169519171140087E-4
38-39	5.474340402126892E-4	3.5641687256980776E-5	4.0653799527493696E-4	1.559323817492909E-5	2.628574435202332E-4
40-41	6.220588229069925E-4	3.5641687256980776E-5	4.605574275237985E-4	1.559323817492909E-5	2.706540626076978E-4
42-43	6.883300851504412E-4	3.5641687256980776E-5	4.828334820594115E-4	1.6707040901709738E-5	2.767799776049913E-4
44-45	7.640686705715254E-4	4.0653799527493696E-5	4.956422134173889E-4	1.8934646355271035E-5	2.8401969532906553E-4
46-47	8.403641573559999E-4	4.121070089088402E-5	5.201458734065632E-4	2.0048449082051685E-5	2.951577225968721E-4
48-49	9.205579536842066E-4	4.3995207707835647E-5	5.591289688438859E-4	2.1162251808832336E-5	3.2021828394943665E-4
50-51	0.0010007517500124133	4.455210907122597E-5	5.674824892947408E-4	2.2276054535612986E-5	3.720101107447368E-4
52-53	0.0011026646995128428	4.455210907122597E-5	6.070224860954538E-4	2.2276054535612986E-5	3.9317236255356915E-4
54-55	0.001206248353103443	4.51090104346163E-5	6.393227651720927E-4	2.2276054535612986E-5	3.9651377073391113E-4
56-57	0.001290340458975382	5.234872815869051E-5	6.938990987843444E-4	2.283295589900331E-5	4.0765179800171763E-4
58-59	0.0014006069289266665	5.2905629522080836E-5	7.217441669538608E-4	2.4503659989174283E-5	4.199036279963047E-4
60-61	0.0015309218479600023	5.4019432248861486E-5	8.09734582369532E-4	2.4503659989174283E-5	4.210174307230854E-4
62-63	0.0016423021206380672	5.736084042920344E-5	9.083061236896195E-4	2.673126544273558E-5	4.210174307230854E-4
64-65	0.001744771971501887	6.237295269971636E-5	9.617686545750905E-4	2.673126544273558E-5	4.321554579908919E-4
66-67	0.0018800990028057358	6.460055815327765E-5	9.690083722991647E-4	2.8401969532906556E-5	4.3549686617123386E-4
68-69	0.0020165398368363653	6.682816360683895E-5	9.795894982035811E-4	3.0072673623077528E-5	4.3995207707835647E-4
70-71	0.002151309966776824	7.128337451396155E-5	9.82930906383923E-4	3.0072673623077528E-5	4.527608084363339E-4
72-73	0.0022983319267118694	7.128337451396155E-5	0.0010157880868239522	3.118647634985818E-5	4.644557370675307E-4
74-75	0.0024548212098245512	7.128337451396155E-5	0.001020800199094465	3.118647634985818E-5	4.728092575183856E-4
76-77	0.002584022326131106	7.406788133091318E-5	0.0010285968181819297	3.118647634985818E-5	5.123492543190986E-4
78-79	0.002695402598809171	7.852309223803577E-5	0.0010347227331792231	3.118647634985818E-5	5.156906624994406E-4
80-81	0.0027895189292221362	8.242140178176804E-5	0.0010430762536300779	3.118647634985818E-5	5.246010843136858E-4
82-83	0.002862473007826268	8.353520450854869E-5	0.0010558849849880555	3.118647634985818E-5	5.429788293055664E-4
84-85	0.0030022552500372397	8.464900723532934E-5	0.00106368160407552	3.174337771324851E-5	5.491047443028601E-4
86-87	0.0030679696109172983	8.576280996210999E-5	0.0010742627299799362	3.2300279076638825E-5	5.641410811143988E-4
88-89	0.0030807783422752756	8.687661268889064E-5	0.0010776041381602782	3.4527884530200126E-5	5.825188261062795E-4
90	0.0030807783422752756	8.799041541567129E-5	0.001085957658611133	3.4527884530200126E-5	5.858602342866215E-4
>>END_MODULE
