##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290307_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80980784
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.06224321068564	37.0	37.0	37.0	37.0	37.0
2	36.08300161677862	37.0	37.0	37.0	37.0	37.0
3	36.15814096094698	37.0	37.0	37.0	37.0	37.0
4	36.12757850059836	37.0	37.0	37.0	37.0	37.0
5	36.108312732561345	37.0	37.0	37.0	37.0	37.0
6	36.020851786270676	37.0	37.0	37.0	37.0	37.0
7	35.97104368611694	37.0	37.0	37.0	37.0	37.0
8	36.0403622296371	37.0	37.0	37.0	37.0	37.0
9	35.99223607170807	37.0	37.0	37.0	37.0	37.0
10-11	36.020714747587526	37.0	37.0	37.0	37.0	37.0
12-13	35.99471395584414	37.0	37.0	37.0	37.0	37.0
14-15	35.98474810394525	37.0	37.0	37.0	37.0	37.0
16-17	35.965744120234746	37.0	37.0	37.0	37.0	37.0
18-19	35.9732490735086	37.0	37.0	37.0	37.0	37.0
20-21	35.963713311296175	37.0	37.0	37.0	37.0	37.0
22-23	35.93488644787632	37.0	37.0	37.0	37.0	37.0
24-25	35.918910262711215	37.0	37.0	37.0	37.0	37.0
26-27	35.889349515312176	37.0	37.0	37.0	37.0	37.0
28-29	35.87188163675965	37.0	37.0	37.0	37.0	37.0
30-31	35.853874864930916	37.0	37.0	37.0	37.0	37.0
32-33	35.834079977541336	37.0	37.0	37.0	37.0	37.0
34-35	35.81533694462627	37.0	37.0	37.0	37.0	37.0
36-37	35.80128456995365	37.0	37.0	37.0	37.0	37.0
38-39	35.78763531215026	37.0	37.0	37.0	37.0	37.0
40-41	35.791264359207474	37.0	37.0	37.0	37.0	37.0
42-43	35.752163244028466	37.0	37.0	37.0	37.0	37.0
44-45	35.73939401780891	37.0	37.0	37.0	37.0	37.0
46-47	35.74415831026951	37.0	37.0	37.0	37.0	37.0
48-49	35.72113861739072	37.0	37.0	37.0	37.0	37.0
50-51	35.70359250609394	37.0	37.0	37.0	37.0	37.0
52-53	35.69120075171553	37.0	37.0	37.0	37.0	37.0
54-55	35.68748039240465	37.0	37.0	37.0	37.0	37.0
56-57	35.65665374867429	37.0	37.0	37.0	37.0	37.0
58-59	35.677503476055605	37.0	37.0	37.0	37.0	37.0
60-61	35.642765042406836	37.0	37.0	37.0	37.0	37.0
62-63	35.62936852242037	37.0	37.0	37.0	37.0	37.0
64-65	35.618025915164196	37.0	37.0	37.0	37.0	37.0
66-67	35.59840485031749	37.0	37.0	37.0	37.0	37.0
68-69	35.56359591391522	37.0	37.0	37.0	37.0	37.0
70-71	35.57133436960113	37.0	37.0	37.0	37.0	37.0
72-73	35.551034717058656	37.0	37.0	37.0	37.0	37.0
74-75	35.56325190927774	37.0	37.0	37.0	37.0	37.0
76-77	35.53761370551139	37.0	37.0	37.0	37.0	37.0
78-79	35.48878977777855	37.0	37.0	37.0	37.0	37.0
80-81	35.48499034916084	37.0	37.0	37.0	37.0	37.0
82-83	35.45380246591694	37.0	37.0	37.0	37.0	37.0
84-85	35.41023254906219	37.0	37.0	37.0	37.0	37.0
86-87	35.37199364901693	37.0	37.0	37.0	37.0	37.0
88-89	35.39920864034377	37.0	37.0	37.0	37.0	37.0
90-91	35.33684433078628	37.0	37.0	37.0	37.0	37.0
92-93	35.38221501548455	37.0	37.0	37.0	37.0	37.0
94-95	35.33365334771787	37.0	37.0	37.0	37.0	37.0
96-97	35.31129872205996	37.0	37.0	37.0	37.0	37.0
98-99	35.307234848885614	37.0	37.0	37.0	37.0	37.0
100-101	35.28945026096761	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12015.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	68.0
12	2634.0
13	19953.0
14	41520.0
15	44839.0
16	40523.0
17	39794.0
18	40297.0
19	44076.0
20	55013.0
21	80599.0
22	113966.0
23	153112.0
24	183902.0
25	219387.0
26	257899.0
27	326298.0
28	414414.0
29	552124.0
30	731571.0
31	1038334.0
32	1512340.0
33	2510209.0
34	4664479.0
35	1.2099403E7
36	4.7038681E7
37	8743334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.756816785519067	16.66590552891697	13.057855587802589	46.51942209776137
2	22.622523602207703	19.55529087470239	30.64885408773767	27.17333143535224
3	24.362318961758103	19.55378447852782	22.930312021451684	33.15358453826239
4	24.79517883519797	23.823823582747806	21.061781486701765	30.31921609535246
5	26.33069952243317	28.050932096260716	23.65483927214329	21.96352910916282
6	22.16090813393839	32.918841280938786	22.722651238737228	22.197599346385598
7	19.521657518180007	22.04626438355581	37.59776748478256	20.83431061348163
8	20.341137442215775	24.266809784502556	29.036143737585025	26.35590903569665
9	21.037018606519755	22.297983806571146	31.491917581209616	25.173080005699482
10-11	22.923157095513567	30.038997949387742	24.099904377299563	22.93794057779913
12-13	22.334010751036267	25.740650223426314	27.34965734250594	24.575681683031476
14-15	22.417853918310975	26.241779414216904	26.915244723679443	24.42512194379268
16-17	23.07493834454176	26.150003899684187	26.40555826341227	24.369499492361776
18-19	22.849673252823393	26.623494890952827	25.91756670767592	24.609265148547863
20-21	22.93411405283179	26.511050460399648	26.143694447574422	24.41114103919414
22-23	22.75730591489788	26.5602314247179	26.32904186341296	24.35342079697126
24-25	22.748616930467698	26.360134033068434	26.4570651980899	24.434183838373972
26-27	22.94242386766588	26.603080828339515	26.2131631867048	24.241332117289797
28-29	22.79418658215848	26.408003193079992	26.26663513571606	24.53117508904547
30-31	22.796848339907527	26.11986360901878	26.410124396050925	24.67316365502277
32-33	23.034024923818404	26.33663833864086	26.243679988619707	24.385656748921033
34-35	22.970830420574178	26.35540262331378	26.160566024470487	24.51320093164156
36-37	22.665644287777564	26.34303692615178	26.337200013589612	24.654118772481045
38-39	23.085023536694315	26.45811962530722	26.274184040800314	24.18267279719815
40-41	23.17632311643616	26.354379770199948	25.937709485306293	24.531587628057597
42-43	22.929298332658227	26.47832942886475	26.164830553820984	24.427541684656042
44-45	23.126700700881283	26.425698779437173	26.286771895088208	24.160828624593332
46-47	23.048022915671638	26.16567503164266	26.27457948033741	24.51172257234829
48-49	22.845970273919335	26.042207363871363	26.399917815620526	24.71190454658878
50-51	23.06607790240257	26.09641780647981	26.236264833328963	24.601239457788655
52-53	23.31074700068669	26.284914674140413	25.965326210434565	24.439012114738333
54-55	22.854401692967052	26.293600525286674	26.27208893947703	24.579908842269248
56-57	23.04283075286897	26.484467014769024	26.158544735132498	24.31415749722951
58-59	23.047751291199432	26.301753131333832	26.118649615477736	24.531845961988996
60-61	23.046800413728043	26.20639128200214	26.080217979351538	24.66659032491828
62-63	23.200588126214235	26.126997531400235	26.148059756350445	24.524354586035077
64-65	23.21798389122072	26.284625591847643	25.90315461159148	24.59423590534016
66-67	23.203069436021682	26.1709596809548	26.02854723526618	24.59742364775734
68-69	23.207490404271333	26.32013833013241	26.155226532940425	24.317144732655834
70-71	23.53022507293391	26.239136279774783	25.97952449906463	24.25111414822667
72-73	23.237955142070284	26.197059572646385	25.95534132209855	24.60964396318478
74-75	23.410236683014766	26.269957301630527	26.017541942758353	24.302264072596355
76-77	23.39798928267812	26.241864478795907	25.96589843947011	24.394247799055858
78-79	23.211432358077168	26.30077930379943	25.879461239171853	24.60832709895155
80-81	23.459762825910317	26.262155068893712	25.87314812258211	24.404933982613862
82-83	23.466549940276547	26.362822568112342	25.613156857409052	24.557470634202055
84-85	23.298077641729883	26.383021268414176	25.792056839363198	24.526844250492747
86-87	23.402531896497123	26.35989570926211	25.766800222197183	24.470772172043585
88-89	23.543741452618676	26.100742991691263	25.69890604398017	24.65660951170989
90-91	23.415454417888736	26.084918679931956	26.00034073261171	24.499286169567593
92-93	23.761927653912366	26.047222278435733	25.924976290316593	24.265873777335308
94-95	23.575298350041848	26.1114970185356	25.90418917575445	24.409015455668104
96-97	23.547002032156936	25.92075634976212	25.98793146339082	24.544310154690123
98-99	23.798693593122596	25.091144852690984	26.420099734056922	24.690061820129493
100-101	24.712574374702182	11.648619118541992	32.241599687709375	31.397206819046453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12234.0
1	6276.0
2	557.0
3	1161.5
4	1998.0
5	2876.0
6	3655.0
7	4315.0
8	4930.5
9	5554.0
10	6215.0
11	6900.5
12	7433.0
13	8002.5
14	8648.0
15	9165.5
16	9763.0
17	10487.0
18	11309.5
19	12441.5
20	13747.0
21	15490.0
22	18386.0
23	22615.5
24	27907.5
25	34528.5
26	41424.0
27	52331.5
28	68622.5
29	87322.0
30	116243.0
31	156527.5
32	224021.5
33	322086.0
34	441779.5
35	589455.0
36	811109.5
37	1122608.0
38	1502409.5
39	2007725.0
40	2508249.0
41	2959427.5
42	3461870.5
43	3980562.0
44	4402479.5
45	4707356.0
46	4863573.5
47	4858860.5
48	4748978.5
49	4559619.0
50	4269359.0
51	3949690.5
52	3551594.0
53	3016114.5
54	2549764.0
55	2226347.0
56	1967575.0
57	1678823.0
58	1392230.0
59	1122467.0
60	933462.0
61	830831.5
62	767440.5
63	709456.0
64	609023.0
65	509476.0
66	446493.0
67	387700.0
68	315054.5
69	244342.0
70	182378.5
71	134284.0
72	102632.0
73	81474.5
74	70309.5
75	52989.0
76	31958.5
77	24166.5
78	18365.5
79	13586.5
80	11483.0
81	8699.0
82	5413.5
83	3466.5
84	3067.5
85	2736.5
86	2425.5
87	2180.5
88	2048.0
89	1873.0
90	1693.0
91	1613.5
92	1601.5
93	1555.0
94	1552.0
95	1546.0
96	1560.0
97	1685.5
98	1914.0
99	2684.0
100	13876.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060890988657259726
2	0.030360288929778698
3	0.015560481607587303
4	0.015395010253296633
5	0.015349320401738763
6	0.01626064771119035
7	0.014841792591190523
8	0.015483920234706544
9	0.014836853147778861
10-11	0.014867107238675289
12-13	0.014856610921425508
14-15	0.014849819186734474
16-17	0.01566914936264386
18-19	0.014899831051277547
20-21	0.014884395290616106
22-23	0.01662184451066811
24-25	0.017347942692182382
26-27	0.01911688086398373
28-29	0.020930274026490038
30-31	0.022531271122294888
32-33	0.02444345315303443
34-35	0.02579871293910911
36-37	1.8589645700745465E-4
38-39	5.008780899353106E-4
40-41	6.31204206196602E-4
42-43	0.0010944362007279742
44-45	8.523428013054038E-4
46-47	5.824460039201149E-4
48-49	5.423092070817405E-4
50-51	9.697521398152315E-4
52-53	3.5702490479727075E-4
54-55	2.5943614346245505E-5
56-57	8.956938449747423E-5
58-59	1.914978831607788E-5
60-61	4.633135196997758E-5
62-63	2.4710769554461304E-6
64-65	1.6062479685210488E-5
66-67	6.795871799486493E-6
68-69	1.9152654085527093E-5
70-71	3.3981720207070514E-5
72-73	0.0
74-75	8.032663826456795E-6
76-77	3.274988142534677E-5
78-79	5.376151093426636E-5
80-81	2.688203423657566E-4
82-83	0.001566034353824932
84-85	1.7490578820236603E-4
86-87	0.0012621168705017347
88-89	5.22309769512487E-4
90-91	0.0022081171561189336
92-93	9.589695688350397E-4
94-95	0.001046907775773008
96-97	0.0017900081780650354
98-99	0.0016551899062323262
100-101	3.415765911932367E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	21463.0
36-37	1145.0
38-39	1446.0
40-41	1426.0
42-43	1592.0
44-45	1624.0
46-47	1553.0
48-49	1561.0
50-51	1786.0
52-53	1915.0
54-55	1965.0
56-57	1929.0
58-59	2086.0
60-61	2314.0
62-63	2427.0
64-65	2433.0
66-67	2691.0
68-69	2800.0
70-71	3155.0
72-73	3088.0
74-75	3242.0
76-77	3348.0
78-79	3668.0
80-81	4104.0
82-83	4195.0
84-85	4718.0
86-87	5039.0
88-89	6035.0
90-91	15068.0
92-93	10232.0
94-95	34566.0
96-97	596046.0
98-99	7275834.0
100-101	7.295429E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.107744576670083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.291325177089035	11.318915885431842
2	15.21282916175925	6.117913661463461
3	8.179569564311658	4.934180866388503
4	5.129060059463746	4.125353183763892
5	3.2909095764416842	3.308638459400346
6	2.23257600897511	2.6935240881883806
7	1.5942395972736507	2.2439593831194364
8	1.14787389928104	1.8464924138375578
9	0.8808490025469194	1.5940698078445268
>10	5.190912182628563	19.381201012631983
>50	0.44710919066772603	6.230995830014834
>100	0.3283272219830629	13.279808037700292
>500	0.039366954724799515	5.475600501859852
>1k	0.03205010892312089	12.371344809854776
>5k	0.002393533871000664	3.207504748294717
>10k+	6.087600596764695E-4	1.8704973102056162
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2348608529154274E-6	1.2348608529154274E-6	7.409165117492565E-6	0.0	2.4697217058308547E-6
2	2.4697217058308547E-6	1.2348608529154274E-6	7.409165117492565E-6	0.0	2.4697217058308547E-6
3	2.4697217058308547E-6	3.7045825587462823E-6	7.409165117492565E-6	0.0	2.4697217058308547E-6
4	6.1743042645771375E-6	3.7045825587462823E-6	7.409165117492565E-6	0.0	2.4697217058308547E-6
5	1.7288051940815985E-5	3.7045825587462823E-6	7.409165117492565E-6	0.0	3.7045825587462823E-6
6	3.210638217580111E-5	3.7045825587462823E-6	1.481833023498513E-5	0.0	3.7045825587462823E-6
7	3.210638217580111E-5	4.9394434116617095E-6	1.7288051940815985E-5	0.0	1.2348608529154275E-5
8	3.704582558746282E-5	6.1743042645771375E-6	1.7288051940815985E-5	0.0	1.3583469382069701E-5
9	4.1985268999124535E-5	6.1743042645771375E-6	1.7288051940815985E-5	1.2348608529154274E-6	1.3583469382069701E-5
10-11	6.174304264577138E-5	1.1113747676238847E-5	1.975777364664684E-5	1.2348608529154274E-6	2.161006492601998E-5
12-13	9.199713354219935E-5	1.2348608529154275E-5	2.4697217058308546E-5	1.2348608529154274E-6	2.346235620539312E-5
14-15	1.1978150273279646E-4	1.3583469382069701E-5	3.395867345517425E-5	1.2348608529154274E-6	2.346235620539312E-5
16-17	1.4139156765881643E-4	1.3583469382069701E-5	3.828068644037825E-5	1.2348608529154274E-6	2.346235620539312E-5
18-19	1.5250531533505529E-4	1.3583469382069701E-5	4.0132977719751394E-5	1.2348608529154274E-6	2.346235620539312E-5
20-21	1.6423649343775184E-4	1.3583469382069701E-5	5.001186454307481E-5	1.2348608529154274E-6	3.210638217580112E-5
22-23	1.7658510196690613E-4	1.3583469382069701E-5	6.174304264577138E-5	1.2348608529154274E-6	3.334124302871654E-5
24-25	2.0807405371624953E-4	1.3583469382069701E-5	6.421276435160224E-5	2.4697217058308547E-6	3.58109647345474E-5
26-27	2.463547401566278E-4	1.7288051940815982E-5	6.915220776326394E-5	2.4697217058308547E-6	3.828068644037825E-5
28-29	2.914271612880409E-4	1.9757773646646838E-5	7.100449904263708E-5	2.4697217058308547E-6	4.939443411661709E-5
30-31	3.3217756943425E-4	2.1610064926019982E-5	7.59439424542988E-5	3.7045825587462823E-6	5.495130795473652E-5
32-33	3.6613624288942426E-4	2.2227495352477694E-5	8.08833858659605E-5	6.7917346910348515E-6	5.803846008702509E-5
34-35	3.957729033593945E-4	2.2227495352477694E-5	1.0866775505655761E-4	7.409165117492565E-6	5.803846008702509E-5
36-37	4.4763705918184245E-4	2.2227495352477694E-5	1.1607692017405017E-4	7.409165117492565E-6	5.927332093994052E-5
38-39	5.211112799303104E-4	2.2227495352477694E-5	1.2039893315925418E-4	8.644025970407991E-6	6.668248605743308E-5
40-41	5.742102966056737E-4	2.2227495352477694E-5	1.3768698510007016E-4	9.878886823323419E-6	7.038706861617936E-5
42-43	6.316313262662411E-4	2.2227495352477694E-5	1.5559246746734386E-4	1.4200899808527414E-5	7.038706861617936E-5
44-45	6.865826342209776E-4	2.2227495352477694E-5	1.5867961959963243E-4	1.6053191087900556E-5	7.285679032201022E-5
46-47	7.619091462488187E-4	2.4697217058308546E-5	1.6917593684941356E-4	2.0992634499562264E-5	8.458796842470678E-5
48-49	8.551411406439335E-4	2.7166938764139402E-5	1.9634287561355298E-4	2.2227495352477694E-5	8.952741183636849E-5
50-51	9.607217435682026E-4	2.7166938764139402E-5	2.0313461030458782E-4	2.2844925778935405E-5	9.570171610094563E-5
52-53	0.0010625977639337255	2.7166938764139402E-5	2.2104009267186152E-4	2.346235620539312E-5	9.817143780677649E-5
54-55	0.0011620040625934173	2.7166938764139402E-5	2.377107141862198E-4	2.346235620539312E-5	1.0619803335072676E-4
56-57	0.0012706718176499748	2.8401799617054832E-5	2.506767531418318E-4	2.469721705830855E-5	1.2286865486508503E-4
58-59	0.0014028019289119256	2.8401799617054832E-5	2.556161965534935E-4	2.469721705830855E-5	1.4756587192339357E-4
60-61	0.001511469683968483	2.8401799617054832E-5	2.704345267884786E-4	2.469721705830855E-5	1.518878849085976E-4
62-63	0.0016250768824367025	2.8401799617054832E-5	2.957491742732449E-4	2.469721705830855E-5	1.518878849085976E-4
64-65	0.0017559721328457378	2.9019230043512547E-5	3.130372262140609E-4	2.469721705830855E-5	1.6361906301129415E-4
66-67	0.0018646398879022955	2.963666046997026E-5	3.142720870669763E-4	2.5314647484766265E-5	1.6855850642295584E-4
68-69	0.001998622290443619	2.963666046997026E-5	3.1921153047863803E-4	2.5932077911223976E-5	1.7411538026107525E-4
70-71	0.0021505101753522167	2.963666046997026E-5	3.216812521844688E-4	2.5932077911223976E-5	1.8337683665794097E-4
72-73	0.0023246255556132922	2.963666046997026E-5	3.340298607136231E-4	2.5932077911223976E-5	1.9696030604001067E-4
74-75	0.0025246730137855914	2.963666046997026E-5	3.3464729114008083E-4	2.5932077911223976E-5	2.0436947115750324E-4
76-77	0.0026648297205914924	2.963666046997026E-5	3.3526472156653856E-4	2.5932077911223976E-5	2.1363092755436895E-4
78-79	0.0027685580322363884	3.0254090896427973E-5	3.358821519929963E-4	2.6549508337681688E-5	2.1795294053957294E-4
80-81	0.002879695508998777	3.087152132288569E-5	3.469958996692351E-4	2.7784369190597117E-5	2.216575230983192E-4
82-83	0.002944525703776837	3.087152132288569E-5	3.488481909486083E-4	3.087152132288568E-5	2.2474467523060778E-4
84-85	0.0030402274198777822	3.087152132288569E-5	3.5193534308089686E-4	3.210638217580111E-5	2.2968411864226953E-4
86-87	0.0030791255367446186	3.087152132288569E-5	3.5502249521318543E-4	3.210638217580111E-5	2.4079786631850836E-4
88-89	0.003082212688876907	3.087152132288569E-5	3.5687478649255855E-4	3.395867345517425E-5	2.506767531418318E-4
90	0.003082212688876907	3.087152132288569E-5	3.5810964734547395E-4	3.457610388163197E-5	2.605556399651552E-4
>>END_MODULE
