##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290289_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72507403
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.40654585298	37.0	37.0	37.0	37.0	37.0
2	36.388029798833095	37.0	37.0	37.0	37.0	37.0
3	36.44392798070564	37.0	37.0	37.0	37.0	37.0
4	36.42234840489322	37.0	37.0	37.0	37.0	37.0
5	36.39865236933117	37.0	37.0	37.0	37.0	37.0
6	36.32894003940535	37.0	37.0	37.0	37.0	37.0
7	36.311355462558765	37.0	37.0	37.0	37.0	37.0
8	36.376813619983054	37.0	37.0	37.0	37.0	37.0
9	36.3377833433091	37.0	37.0	37.0	37.0	37.0
10-11	36.35878231633809	37.0	37.0	37.0	37.0	37.0
12-13	36.34540581325192	37.0	37.0	37.0	37.0	37.0
14-15	36.33824493755486	37.0	37.0	37.0	37.0	37.0
16-17	36.32409889235724	37.0	37.0	37.0	37.0	37.0
18-19	36.32989067337028	37.0	37.0	37.0	37.0	37.0
20-21	36.325403097115476	37.0	37.0	37.0	37.0	37.0
22-23	36.29796185777058	37.0	37.0	37.0	37.0	37.0
24-25	36.2852463078839	37.0	37.0	37.0	37.0	37.0
26-27	36.26214472196722	37.0	37.0	37.0	37.0	37.0
28-29	36.25083058760221	37.0	37.0	37.0	37.0	37.0
30-31	36.23431710138618	37.0	37.0	37.0	37.0	37.0
32-33	36.214183860646614	37.0	37.0	37.0	37.0	37.0
34-35	36.202581631561124	37.0	37.0	37.0	37.0	37.0
36-37	36.18741262867184	37.0	37.0	37.0	37.0	37.0
38-39	36.17506091161459	37.0	37.0	37.0	37.0	37.0
40-41	36.17425206581154	37.0	37.0	37.0	37.0	37.0
42-43	36.14573805459155	37.0	37.0	37.0	37.0	37.0
44-45	36.13735425774499	37.0	37.0	37.0	37.0	37.0
46-47	36.1364964378823	37.0	37.0	37.0	37.0	37.0
48-49	36.11511009877592	37.0	37.0	37.0	37.0	37.0
50-51	36.09904600387996	37.0	37.0	37.0	37.0	37.0
52-53	36.08873355591311	37.0	37.0	37.0	37.0	37.0
54-55	36.08324717434685	37.0	37.0	37.0	37.0	37.0
56-57	36.0555849205393	37.0	37.0	37.0	37.0	37.0
58-59	36.072674630286144	37.0	37.0	37.0	37.0	37.0
60-61	36.044559067560016	37.0	37.0	37.0	37.0	37.0
62-63	36.03382521256191	37.0	37.0	37.0	37.0	37.0
64-65	36.02375371665559	37.0	37.0	37.0	37.0	37.0
66-67	36.006707537014535	37.0	37.0	37.0	37.0	37.0
68-69	35.97868330926112	37.0	37.0	37.0	37.0	37.0
70-71	35.980956984241786	37.0	37.0	37.0	37.0	37.0
72-73	35.960299266043236	37.0	37.0	37.0	37.0	37.0
74-75	35.969467437243935	37.0	37.0	37.0	37.0	37.0
76-77	35.9488438199339	37.0	37.0	37.0	37.0	37.0
78-79	35.911729704745035	37.0	37.0	37.0	37.0	37.0
80-81	35.90815294209226	37.0	37.0	37.0	37.0	37.0
82-83	35.88115632593119	37.0	37.0	37.0	37.0	37.0
84-85	35.842133785363686	37.0	37.0	37.0	37.0	37.0
86-87	35.8090640462406	37.0	37.0	37.0	37.0	37.0
88-89	35.82685377380223	37.0	37.0	37.0	37.0	37.0
90-91	35.775810045354035	37.0	37.0	37.0	37.0	37.0
92-93	35.80798320367609	37.0	37.0	37.0	37.0	37.0
94-95	35.76408947587623	37.0	37.0	37.0	37.0	37.0
96-97	35.74028430254525	37.0	37.0	37.0	37.0	37.0
98-99	35.73527601088096	37.0	37.0	37.0	37.0	37.0
100-101	35.70115249782464	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6170.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	21.0
12	421.0
13	2720.0
14	6150.0
15	8879.0
16	10654.0
17	13685.0
18	16962.0
19	21404.0
20	27207.0
21	37779.0
22	53093.0
23	73812.0
24	94292.0
25	115594.0
26	139086.0
27	177663.0
28	230484.0
29	311722.0
30	418856.0
31	604655.0
32	889105.0
33	1491650.0
34	2851103.0
35	8108765.0
36	4.4239937E7
37	1.2555534E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.137094386890666	15.502272795140875	15.221092673773157	49.1395401441953
2	22.250000334529236	20.246032252177603	33.47925981495333	24.024707598339837
3	24.06248913373055	21.890655919299466	23.341106082334367	30.705748864635616
4	24.35127414438542	26.664526920457853	20.61788413122557	28.366314803931157
5	26.24893570272034	30.82271955558063	22.96492636159363	19.963418380105402
6	20.77723902079194	35.06685083162506	22.60103900374371	21.55487114383929
7	18.750703953574305	20.558767071951745	39.980640328446846	20.709888646027107
8	19.950397221260125	23.659169887241347	29.500969348231948	26.88946354326658
9	20.87879859584729	22.11726584338961	31.66235618813148	25.341579372631617
10-11	23.1362038904426	30.950724395486308	23.212636406859467	22.700435307211627
12-13	22.01808886864555	25.843924620989984	27.475888249738702	24.66209826062576
14-15	22.28943243408683	26.496072797011493	26.871980274869312	24.342514494032365
16-17	23.1143687539754	26.6006336635903	26.16776423206893	24.11723335036537
18-19	22.878784670898828	26.968542652095962	25.878810808402648	24.273861868602562
20-21	23.10092411517022	26.907167820920126	26.110876068993655	23.881031994916
22-23	22.795929548344155	26.96907704713738	26.39059508588919	23.844398318629278
24-25	22.5653134720452	26.999162001907166	26.513015522102606	23.922509003945024
26-27	22.7827589866382	27.045203627872127	26.417053927567192	23.75498345792248
28-29	22.745952452994914	26.935162025641368	26.278139552648323	24.0407459687154
30-31	22.695562276582933	26.938290486938964	26.326396419350225	24.039750817127878
32-33	22.969206630877306	26.994361610599398	26.193565025942185	23.842866732581115
34-35	22.9744415397535	26.85366909733756	26.123373973871022	24.048515389037924
36-37	22.777395609023667	26.841419882627708	26.277515227816885	24.103669280531737
38-39	23.10595732625195	26.981589758273984	26.163528838602247	23.74892407687182
40-41	23.11431620534571	26.81036559907355	26.11413371735865	23.961184478222094
42-43	22.91049082727723	26.72939579614926	26.36657001350492	23.993543363068593
44-45	23.036825848636237	26.78720286640554	26.371046569323152	23.804924715635075
46-47	22.989651723899225	26.749465180791198	26.265151578484026	23.99573151682555
48-49	22.851482944523227	26.699941901901152	26.282958986362136	24.165616167213486
50-51	23.165668276778906	26.80557929192549	26.115623719236215	23.913128712059386
52-53	23.325570371256173	26.776802183640648	25.997067103680283	23.9005603414229
54-55	22.904546988808768	26.711649511960662	26.329469825949577	24.054333673281
56-57	23.095310594280942	26.847215539439556	26.29601304107284	23.76146082520666
58-59	23.181926599750426	26.670959271755194	26.2455736002345	23.90154052825988
60-61	23.07230648497951	26.62687240508106	26.241142369581794	24.059678740357636
62-63	23.18416072618242	26.66693801878414	26.33309212889603	23.81580912613741
64-65	23.23341478313399	26.712399949460842	26.189792665963584	23.86439260144158
66-67	23.15486207534085	26.691999227124448	26.268842549870598	23.884296147664102
68-69	23.35626876791385	26.77185151392214	26.169417490401198	23.702462227762812
70-71	23.431541402962875	26.7354033710235	26.062844814018078	23.770210411995546
72-73	23.18818315324205	26.744359207420242	26.217527485108437	23.849930154229266
74-75	23.26422452778122	26.737135566634585	26.323836617260945	23.674803288323247
76-77	23.30145734203357	26.67462510593237	26.205945153432914	23.817972398601153
78-79	23.134620806228607	26.68741184961112	26.312357629696255	23.865609714464018
80-81	23.40481927308343	26.761759349106534	26.19040092165014	23.643020456159892
82-83	23.41187920539837	26.73941503628306	26.088152826527516	23.76055293179105
84-85	23.267731249709577	26.72321275870181	26.191171181509315	23.8178848100793
86-87	23.382200714763457	26.724028719599264	26.228743477753063	23.665027087884212
88-89	23.509453524052702	26.528832152441538	26.187578870434663	23.774135453071096
90-91	23.27939728413168	26.58513463081384	26.333965704074608	23.801502380979876
92-93	23.44582188506829	26.647328541504745	26.25333907250283	23.653510500924135
94-95	23.518618384586393	26.597615980698453	26.191798160872754	23.6919674738424
96-97	23.3974076145383	26.488200839390714	26.301825066617234	23.812566479453753
98-99	23.60898507537203	25.620155776532705	26.77104132074806	23.999817827347204
100-101	24.353132987450106	12.065721251798363	33.16285481841968	30.418290942331854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6272.0
1	3171.0
2	144.0
3	325.0
4	526.0
5	710.5
6	858.5
7	977.5
8	1091.5
9	1151.0
10	1238.5
11	1362.0
12	1497.0
13	1673.0
14	1863.5
15	2181.0
16	2598.0
17	3145.5
18	3883.0
19	4872.0
20	6258.5
21	7979.5
22	10414.5
23	14295.0
24	19960.5
25	27480.5
26	38038.0
27	54353.5
28	77742.0
29	106849.5
30	149355.0
31	209547.5
32	295876.0
33	408440.0
34	553047.5
35	742930.5
36	984143.5
37	1280715.5
38	1645319.5
39	2068921.0
40	2500449.0
41	2924392.0
42	3356915.5
43	3734164.5
44	4016308.0
45	4212651.5
46	4311136.0
47	4315457.0
48	4166472.5
49	3890554.5
50	3566756.5
51	3232658.0
52	2913981.0
53	2578858.5
54	2239466.0
55	1943112.0
56	1672699.5
57	1385098.5
58	1106494.0
59	893009.5
60	753758.5
61	651232.0
62	570859.0
63	506104.5
64	444609.5
65	394132.5
66	351234.5
67	307597.0
68	250061.0
69	187001.0
70	139980.5
71	106735.5
72	78175.0
73	57330.5
74	44197.0
75	32817.5
76	21030.5
77	12201.5
78	7329.0
79	4619.0
80	3387.0
81	2737.5
82	2214.5
83	1841.0
84	1691.5
85	1627.0
86	1560.5
87	1475.0
88	1451.0
89	1454.0
90	1424.0
91	1389.0
92	1446.0
93	1498.5
94	1509.0
95	1507.0
96	1375.5
97	1101.0
98	890.0
99	936.0
100	5349.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05611427015252498
2	0.024102366485253927
3	0.0091990606807418
4	0.00909286462790565
5	0.008982531066517443
6	0.00990657464314368
7	0.00851361343061756
8	0.009138377221978286
9	0.008512234261100208
10-11	0.008539817651447259
12-13	0.008530853049584468
14-15	0.008530163464825791
16-17	0.009361802683789406
18-19	0.008575676058898427
20-21	0.008568090626552989
22-23	0.01019137314847699
24-25	0.009982428966598072
26-27	0.011095418767101616
28-29	0.012265644102575291
30-31	0.013410354801977943
32-33	0.015036395662936653
34-35	0.01605767069053625
36-37	2.1449744799764562E-4
38-39	5.683230995172095E-4
40-41	6.855840030767134E-4
42-43	0.0011697868742808458
44-45	9.628827993679184E-4
46-47	6.242289005579151E-4
48-49	5.952695166198388E-4
50-51	0.001027083714710302
52-53	3.800771740148783E-4
54-55	2.0694301828130484E-5
56-57	1.03473753954741E-4
58-59	1.931554643763653E-5
60-61	4.829013230261403E-5
62-63	4.829147620192258E-6
64-65	1.6557545771024126E-5
66-67	6.89919568624754E-6
68-69	1.8628498464784045E-5
70-71	2.4839115839252145E-5
72-73	0.0
74-75	9.660740731499142E-6
76-77	3.588528649839697E-5
78-79	5.728333441458503E-5
80-81	2.8575777453722633E-4
82-83	0.0016057125702980755
84-85	1.8366036431753644E-4
86-87	0.001294874595608074
88-89	5.622900806724624E-4
90-91	0.0022872152359159903
92-93	0.0010487747528211782
94-95	0.0011011879459236205
96-97	0.0018968655626738205
98-99	0.0017220425318674735
100-101	3.6345052768874353E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	12112.0
36-37	1044.0
38-39	1053.0
40-41	1062.0
42-43	1202.0
44-45	1183.0
46-47	1274.0
48-49	1287.0
50-51	1522.0
52-53	1542.0
54-55	1592.0
56-57	1600.0
58-59	1860.0
60-61	1987.0
62-63	2062.0
64-65	2202.0
66-67	2485.0
68-69	3096.0
70-71	3698.0
72-73	4186.0
74-75	4860.0
76-77	5860.0
78-79	7400.0
80-81	9452.0
82-83	11923.0
84-85	14849.0
86-87	17399.0
88-89	21763.0
90-91	34506.0
92-93	34889.0
94-95	59207.0
96-97	561227.0
98-99	6723273.0
100-101	6.4952746E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.209672092427933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.34827425283082	15.060083933012983
2	17.325068205938766	9.42818849725085
3	8.38430210726387	6.844023331875074
4	5.037701275768137	5.482967992530275
5	3.129858426532624	4.258121074083757
6	2.048247320316474	3.3439282770003285
7	1.4162050666999741	2.6974132836378932
8	1.1237303411367254	2.4461067282113986
9	0.8396607813804415	2.056220507721027
>10	4.773579673656504	23.793433133505417
>50	0.3462351254780044	6.5229474988672695
>100	0.2013742893897031	10.428069558760548
>500	0.017262331083362115	3.232015388739061
>1k	0.00822753489789657	3.861230695566175
>5k	2.376240233502722E-4	0.4167190103874847
>10k+	3.564360350254083E-5	0.12853108885033296
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3791695173525937E-6	0.0	0.0	0.0	5.516678069410375E-6
2	2.7583390347051874E-6	0.0	2.7583390347051874E-6	5.516678069410375E-6	9.654186621468156E-6
3	8.275017104115561E-6	0.0	2.7583390347051874E-6	5.516678069410375E-6	1.3791695173525936E-5
4	3.034172938175706E-5	0.0	1.3791695173525936E-5	5.516678069410375E-6	2.3445881794994092E-5
5	3.8616746485872625E-5	0.0	2.4825051312346686E-5	5.516678069410375E-6	2.4825051312346686E-5
6	4.27542550379304E-5	0.0	4.137508552057781E-5	5.516678069410375E-6	2.4825051312346686E-5
7	4.6891763589988185E-5	2.7583390347051874E-6	6.48209673155719E-5	5.516678069410375E-6	3.1720898899109655E-5
8	5.654595021145634E-5	4.137508552057781E-6	6.48209673155719E-5	5.516678069410375E-6	3.447923793381484E-5
9	5.792511972880893E-5	4.137508552057781E-6	6.75793063502771E-5	5.516678069410375E-6	3.8616746485872625E-5
10-11	6.895847586762968E-5	4.137508552057781E-6	7.585432345439265E-5	6.895847586762968E-6	4.551259407263559E-5
12-13	8.343975579983191E-5	6.895847586762968E-6	1.0343771380144453E-4	6.895847586762968E-6	5.171885690072226E-5
14-15	1.061960528361497E-4	9.654186621468156E-6	1.4895030787408011E-4	9.654186621468156E-6	5.6545950211456334E-5
16-17	1.1516065469894157E-4	9.654186621468156E-6	2.0480667332686015E-4	1.103335613882075E-5	5.8614704487485226E-5
18-19	1.2412525656173344E-4	1.103335613882075E-5	2.1652961422435722E-4	1.103335613882075E-5	6.137304352219042E-5
20-21	1.3171068890717268E-4	1.2412525656173343E-5	2.4204425029538017E-4	1.103335613882075E-5	6.275221303954301E-5
22-23	1.4067529076996455E-4	1.310211041484964E-5	2.785922425052239E-4	1.103335613882075E-5	6.75793063502771E-5
24-25	1.703274353930453E-4	1.3791695173525936E-5	2.8066099678125277E-4	1.6550034208231123E-5	6.75793063502771E-5
26-27	2.0687542760288905E-4	1.3791695173525936E-5	2.9307352243742614E-4	1.6550034208231123E-5	7.171681490233486E-5
28-29	2.592838692622876E-4	1.517086469087853E-5	3.3100068416462244E-4	1.7929203725583716E-5	7.585432345439265E-5
30-31	3.172089889910965E-4	1.6550034208231123E-5	3.3789653175138546E-4	2.0687542760288902E-5	7.999183200645043E-5
32-33	3.5996324402902695E-4	1.7929203725583716E-5	3.489298878902062E-4	2.7583390347051872E-5	8.75772643518897E-5
34-35	4.109925161710729E-4	1.9308373242936313E-5	4.2754255037930403E-4	3.103131414043336E-5	9.240435766262378E-5
36-37	4.7236555969326334E-4	1.9308373242936313E-5	4.358175674834196E-4	3.241048365778595E-5	1.0067937476673935E-4
38-39	5.502886374236849E-4	1.9308373242936313E-5	4.516780169329744E-4	3.3100068416462245E-5	1.082648071121786E-4
40-41	6.137304352219041E-4	1.9308373242936313E-5	4.937426872122286E-4	3.3100068416462245E-5	1.1309190042291268E-4
42-43	6.620013683292449E-4	1.9997958001612607E-5	5.240844165939856E-4	3.378965317513854E-5	1.1929816325099936E-4
44-45	7.213056575754064E-4	2.0687542760288906E-5	5.289115099047196E-4	3.8616746485872625E-5	1.2550442607908602E-4
46-47	8.026766590992095E-4	2.0687542760288906E-5	5.509782221823612E-4	3.8616746485872625E-5	1.296419346311438E-4
48-49	8.743934740015444E-4	2.0687542760288906E-5	5.764928582533841E-4	4.4133424555283E-5	1.3722736697658307E-4
50-51	9.578332298013762E-4	2.0687542760288906E-5	5.861470448748523E-4	4.482300931395929E-5	1.4895030787408011E-4
52-53	0.001066787621672231	2.0687542760288906E-5	6.10972096187199E-4	4.8960517866017076E-5	1.5722532497819568E-4
54-55	0.0011812586916124966	2.20667122776415E-5	6.495888426730716E-4	5.171885690072226E-5	1.6067324877157713E-4
56-57	0.0012736630492751204	2.2756297036317794E-5	6.875160044002679E-4	5.240844165939856E-5	1.6963785063436903E-4
58-59	0.0013688257459724491	2.620422082969928E-5	7.006181148151176E-4	5.240844165939856E-5	1.751545287037794E-4
60-61	0.0014591613493590441	2.6893805588375577E-5	7.923328877190651E-4	5.5166780694103744E-5	1.76533698221132E-4
62-63	0.0015646678174365174	2.8962559864404465E-5	8.654288721387525E-4	5.5856365452780046E-5	1.76533698221132E-4
64-65	0.0016722430397900197	3.034172938175706E-5	9.226644071088851E-4	5.7235534970132636E-5	1.8067120677318975E-4
66-67	0.0018315371190442443	3.172089889910965E-5	9.254227461435903E-4	5.8614704487485226E-5	1.8618788484260016E-4
68-69	0.0019549727908473014	3.585840745116743E-5	9.323185937303533E-4	5.930428924616153E-5	1.95152486705392E-4
70-71	0.0020901314035478556	3.585840745116743E-5	9.343873480063822E-4	6.55105520742482E-5	2.1308169043097574E-4
72-73	0.0022597692541822248	3.585840745116743E-5	9.723145097335785E-4	6.688972159160079E-5	2.3101089415655942E-4
74-75	0.0024500946475768826	3.6547992209843733E-5	9.771416030443126E-4	6.964806062630599E-5	2.39285911260675E-4
76-77	0.0025990449554509627	4.27542550379304E-5	9.861062049071044E-4	7.171681490233486E-5	2.5169843691684836E-4
78-79	0.002725238966288725	4.27542550379304E-5	9.930020524938675E-4	7.171681490233486E-5	2.57904699744935E-4
80-81	0.0028445371295397247	4.6891763589988185E-5	9.99208315321954E-4	7.309598441968747E-5	2.627317930556691E-4
82-83	0.002932114393891614	4.758134834866448E-5	0.0010123104257368037	8.137100152380303E-5	2.8066099678125277E-4
84-85	0.00302589792107159	4.8270933107340775E-5	0.0010261021209103296	8.343975579983191E-5	2.9307352243742614E-4
86-87	0.0030672730065921684	4.896051786601707E-5	0.0010357563075317979	8.412934055850821E-5	3.013485395415417E-4
88-89	0.003078306362730989	5.171885690072226E-5	0.0010495480027053238	8.75772643518897E-5	3.075548023696284E-4
90	0.003078306362730989	5.240844165939856E-5	0.0010536855112573814	8.8266849110566E-5	3.1031314140433355E-4
>>END_MODULE
