##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR21290294_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89782506
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.28459883933291	37.0	37.0	37.0	37.0	37.0
2	36.28998647019276	37.0	37.0	37.0	37.0	37.0
3	36.37440663551984	37.0	37.0	37.0	37.0	37.0
4	36.39609548211987	37.0	37.0	37.0	37.0	37.0
5	36.40963570341866	37.0	37.0	37.0	37.0	37.0
6	36.386770681139154	37.0	37.0	37.0	37.0	37.0
7	36.34312636584236	37.0	37.0	37.0	37.0	37.0
8	36.39489449091563	37.0	37.0	37.0	37.0	37.0
9	36.37365883950711	37.0	37.0	37.0	37.0	37.0
10-11	36.39678728726953	37.0	37.0	37.0	37.0	37.0
12-13	36.3722249911358	37.0	37.0	37.0	37.0	37.0
14-15	36.36822720787054	37.0	37.0	37.0	37.0	37.0
16-17	36.36524575288642	37.0	37.0	37.0	37.0	37.0
18-19	36.35902702470791	37.0	37.0	37.0	37.0	37.0
20-21	36.35235311876904	37.0	37.0	37.0	37.0	37.0
22-23	36.336700954860845	37.0	37.0	37.0	37.0	37.0
24-25	36.306412621184805	37.0	37.0	37.0	37.0	37.0
26-27	36.2624177142037	37.0	37.0	37.0	37.0	37.0
28-29	36.24958533681384	37.0	37.0	37.0	37.0	37.0
30-31	36.2392486739009	37.0	37.0	37.0	37.0	37.0
32-33	36.23179950557406	37.0	37.0	37.0	37.0	37.0
34-35	36.2186085393963	37.0	37.0	37.0	37.0	37.0
36-37	36.21425185165627	37.0	37.0	37.0	37.0	37.0
38-39	36.206253524315315	37.0	37.0	37.0	37.0	37.0
40-41	36.203569472378135	37.0	37.0	37.0	37.0	37.0
42-43	36.18758504862773	37.0	37.0	37.0	37.0	37.0
44-45	36.187488660998994	37.0	37.0	37.0	37.0	37.0
46-47	36.181299131142964	37.0	37.0	37.0	37.0	37.0
48-49	36.15366276178716	37.0	37.0	37.0	37.0	37.0
50-51	36.14889749415451	37.0	37.0	37.0	37.0	37.0
52-53	36.14814935161832	37.0	37.0	37.0	37.0	37.0
54-55	36.13672390134104	37.0	37.0	37.0	37.0	37.0
56-57	36.12217204089914	37.0	37.0	37.0	37.0	37.0
58-59	36.10989954600288	37.0	37.0	37.0	37.0	37.0
60-61	36.107621253293544	37.0	37.0	37.0	37.0	37.0
62-63	36.097385811307376	37.0	37.0	37.0	37.0	37.0
64-65	36.08129710455466	37.0	37.0	37.0	37.0	37.0
66-67	36.07525486451743	37.0	37.0	37.0	37.0	37.0
68-69	36.05859330428781	37.0	37.0	37.0	37.0	37.0
70-71	36.03660189825469	37.0	37.0	37.0	37.0	37.0
72-73	36.01591516028992	37.0	37.0	37.0	37.0	37.0
74-75	36.012413723755564	37.0	37.0	37.0	37.0	37.0
76-77	35.9986273702132	37.0	37.0	37.0	37.0	37.0
78-79	35.98429508791999	37.0	37.0	37.0	37.0	37.0
80-81	35.956685912527156	37.0	37.0	37.0	37.0	37.0
82-83	35.96824477377047	37.0	37.0	37.0	37.0	37.0
84-85	35.952103529981905	37.0	37.0	37.0	37.0	37.0
86-87	35.96398040335171	37.0	37.0	37.0	37.0	37.0
88-89	35.93894113939729	37.0	37.0	37.0	37.0	37.0
90-91	35.921353388057376	37.0	37.0	37.0	37.0	37.0
92-93	35.908112755760314	37.0	37.0	37.0	37.0	37.0
94-95	35.88138443986779	37.0	37.0	37.0	37.0	37.0
96-97	35.88057192042438	37.0	37.0	37.0	37.0	37.0
98-99	35.858036543219036	37.0	37.0	37.0	37.0	37.0
100-101	35.81017158102283	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28723.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	36.0
15	437.0
16	2030.0
17	5414.0
18	9483.0
19	13459.0
20	16800.0
21	21315.0
22	28030.0
23	43767.0
24	72135.0
25	120708.0
26	188487.0
27	295129.0
28	432533.0
29	622983.0
30	827377.0
31	1092530.0
32	1389547.0
33	1906830.0
34	2899227.0
35	6633161.0
36	5.2558616E7
37	2.0573748E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.41072445236883	16.464071393541342	16.68266610944194	46.44253804464789
2	22.756607320756892	20.98064482887363	30.40345891329787	25.859288937071607
3	24.87705088356081	21.362539758091643	22.030920255187144	31.729489103160404
4	24.607446882151823	25.38523699119648	20.60469054132396	29.402625585327733
5	26.420693811218054	29.23615271259078	23.289613564010114	21.05353991218105
6	21.765474776701055	33.84881838351036	22.502677129497705	21.883029710290874
7	19.165547595915818	21.572990410888863	38.78142495676199	20.480037036433327
8	20.52409980312473	24.495214870218895	28.64438705608654	26.33629827056983
9	20.957266413099635	22.949799741584144	31.05217818066071	25.040755664655507
10-11	23.0438498620164	30.658309967837234	23.394219494904185	22.903620675242177
12-13	22.207048980258627	25.90778297455775	27.19590947510418	24.689258570079446
14-15	22.36568507835507	26.3793173630763	26.78513294279159	24.46986461577704
16-17	23.04033562933704	26.546939112031776	26.123644938611218	24.289080320019966
18-19	22.82716070551786	26.984655733376854	25.86350446665704	24.324679094448246
20-21	23.054235790327578	26.96023268463032	26.01988988583631	23.965641639205792
22-23	22.78489690428378	26.969033467692167	26.297477371914148	23.94859225610991
24-25	22.600882931810386	26.939242776677673	26.39762375163604	24.062250539875897
26-27	22.886903660320122	26.938573101345938	26.24549621133461	23.92902702699932
28-29	22.823864080523983	26.89958441704069	26.115852747067226	24.160698755368102
30-31	22.69148914449048	26.969416416728514	26.20551304891512	24.13358138986589
32-33	23.01249704236714	26.95735071550968	26.093681530183016	23.93647071194016
34-35	22.994054484501657	26.776575209006936	26.084631594741374	24.144738711750023
36-37	22.82752792467809	26.73833667177647	26.21148281072458	24.222652592820857
38-39	23.17912554569866	26.932771712667254	25.99345204510103	23.894650696533066
40-41	23.159255274465064	26.789915106798023	25.94501622867328	24.10581339006363
42-43	22.94785910914621	26.748862473323754	26.262181673075407	24.04109674445463
44-45	23.10903116994289	26.7276405839999	26.24995426432472	23.913373981732487
46-47	23.057771183493642	26.63482240758382	26.157175793523912	24.150230615398623
48-49	22.9049368890362	26.629200393874925	26.162147547265636	24.303715169823235
50-51	23.20345972092411	26.787378175320026	25.961858892257833	24.047303211498033
52-53	23.312171826290886	26.785683908366753	25.906346600537283	23.995797664805078
54-55	22.952461450025833	26.69149615270605	26.238654154054753	24.117388243213362
56-57	23.145365670105512	26.78635963325066	26.162117516597093	23.90615718004674
58-59	23.21139107340416	26.599509849235687	26.109506196684627	24.079592880675524
60-61	23.099182325272483	26.603190520297893	26.096950185699193	24.20067696873043
62-63	23.210736843412235	26.658565496337083	26.1881314930799	23.94256616717078
64-65	23.25455401617101	26.688324436123956	26.069128330286933	23.9879932174181
66-67	23.199002964959583	26.584792498084674	26.21237738537372	24.003827151582026
68-69	23.43132654728485	26.664463031389317	26.036962047709828	23.867248373616004
70-71	23.46847505190019	26.69647959410049	25.902632991241347	23.932412362757972
72-73	23.20086945591146	26.767444196744194	26.05867913166726	23.973007215677086
74-75	23.290791961032742	26.72517106113264	26.19911443807631	23.78492253975831
76-77	23.329232670722945	26.564661882687297	26.139484552146445	23.966620894443313
78-79	23.173884465798924	26.59429120698016	26.218122757299184	24.013701569921732
80-81	23.450497461970045	26.69295769028952	26.030804253227036	23.825740594513395
82-83	23.423458670661855	26.72957718041571	25.936428027385787	23.91053612153665
84-85	23.265456515110184	26.7430267685009	26.073932158547166	23.917584557841753
86-87	23.42360373955582	26.645665349403462	26.145513679655284	23.785217231385428
88-89	23.547409684117017	26.471351799822752	26.075553516294185	23.905684999766045
90-91	23.344253309760287	26.562168615855807	26.172492728836477	23.92108534554743
92-93	23.46388535533459	26.647284371985663	26.107605460410436	23.781224812269308
94-95	23.474584871273958	26.573177531326404	26.0967963931228	23.85544120427684
96-97	23.381923652343804	26.431792883748845	26.263954505310632	23.922328958596715
98-99	23.63381964852101	25.548169485780537	26.66762312378471	24.150387741913747
100-101	24.33951630122899	12.04181687089238	33.02332672332959	30.59534010454904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29168.0
1	14654.0
2	113.5
3	89.5
4	99.5
5	94.0
6	88.0
7	95.0
8	102.0
9	115.0
10	150.0
11	222.0
12	326.0
13	485.5
14	755.0
15	1096.0
16	1688.5
17	2469.5
18	3471.5
19	4802.0
20	6427.5
21	8644.5
22	12090.5
23	17215.5
24	24070.0
25	32680.0
26	45016.0
27	64389.0
28	92547.5
29	126508.0
30	174974.5
31	243377.5
32	340134.0
33	463425.0
34	619745.0
35	831101.5
36	1112785.0
37	1465711.5
38	1902127.5
39	2412211.5
40	2954549.5
41	3513878.0
42	4076808.5
43	4574966.0
44	4972443.5
45	5254813.5
46	5409700.0
47	5434351.0
48	5262105.5
49	4937896.0
50	4549326.5
51	4131626.5
52	3717648.5
53	3292075.0
54	2854151.5
55	2463951.0
56	2119380.0
57	1760911.0
58	1407235.0
59	1135310.5
60	960689.0
61	829012.0
62	718155.5
63	623906.5
64	539961.5
65	471584.5
66	415905.5
67	363001.0
68	292688.0
69	217093.0
70	164828.0
71	129424.5
72	96732.5
73	73344.5
74	59053.5
75	44729.0
76	28637.5
77	17441.0
78	11485.5
79	7242.5
80	5904.0
81	4608.5
82	2780.0
83	1610.0
84	1290.5
85	1079.5
86	892.5
87	774.0
88	659.0
89	582.0
90	518.5
91	463.5
92	465.5
93	522.0
94	697.5
95	1083.0
96	2260.5
97	6398.5
98	24092.0
99	27967.0
100	9185.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06869155556874298
2	0.04337593339174561
3	0.03378831951961778
4	0.03199843853768127
5	0.03202628360585079
6	0.03199175572132059
7	0.03199175572132059
8	0.03199175572132059
9	0.031993983326774146
10-11	0.03199175572132059
12-13	0.03201458867721959
14-15	0.032083644446279994
16-17	0.032000666143134834
18-19	0.03220783345031604
20-21	0.03201960078949011
22-23	0.03224069063075607
24-25	0.03334168462617873
26-27	0.03435858651572947
28-29	0.035590452331548866
30-31	0.03653662774794903
32-33	0.03763595103928153
34-35	0.03892462079416674
36-37	1.1811041607959662E-4
38-39	1.8385366064254546E-5
40-41	2.1728483114703844E-5
42-43	3.922327092595211E-4
44-45	7.911631797514554E-5
46-47	1.682642071654655E-4
48-49	1.537805442427183E-4
50-51	6.351927294814961E-5
52-53	1.0419628297423709E-4
54-55	3.064670618089512E-5
56-57	0.0
58-59	5.57241526944762E-7
60-61	0.0
62-63	6.130100903634274E-6
64-65	3.901135832974229E-6
66-67	8.359973456972809E-6
68-69	0.0
70-71	5.574072593744907E-7
72-73	6.132067793465714E-6
74-75	0.0
76-77	2.787985833017269E-6
78-79	0.0
80-81	5.578081646626498E-7
82-83	6.695563341739372E-5
84-85	8.372486989657568E-6
86-87	5.025690015093655E-6
88-89	2.994632384040641E-4
90-91	5.5906192986165564E-5
92-93	6.155205188717108E-6
94-95	6.211599857474869E-4
96-97	2.8625740662273576E-5
98-99	2.5175940774697912E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	35696.0
36-37	1164.0
38-39	1365.0
40-41	1293.0
42-43	1367.0
44-45	1445.0
46-47	1514.0
48-49	1603.0
50-51	1999.0
52-53	2218.0
54-55	2065.0
56-57	2409.0
58-59	2870.0
60-61	3383.0
62-63	3668.0
64-65	4070.0
66-67	5009.0
68-69	6423.0
70-71	8184.0
72-73	9739.0
74-75	11266.0
76-77	13875.0
78-79	17817.0
80-81	23533.0
82-83	30353.0
84-85	37651.0
86-87	44216.0
88-89	54030.0
90-91	76413.0
92-93	82579.0
94-95	118290.0
96-97	743052.0
98-99	8392860.0
100-101	8.0039087E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.438860208537502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.728703918949826	10.485388549634536
2	17.69865984742128	7.942755083894835
3	10.295005287821668	6.930245534987545
4	6.448120107649618	5.7875386281363825
5	4.157682425684253	4.664682737071103
6	2.911300870625075	3.919576395656974
7	2.0304741703451694	3.189306824579499
8	1.5011484154188415	2.6947247556680773
9	1.1326855438074208	2.28745553199534
>10	6.341962387860191	25.8653378032419
>50	0.4571361895697022	7.043156615151619
>100	0.2653608568684066	11.2207614371922
>500	0.02154156203684726	3.290169961390194
>1k	0.00985391987538357	3.940544170934112
>5k	2.527956586812335E-4	0.37751186440153967
>10k+	1.1170040732426598E-4	0.36084410606405337
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1138027267806493E-6	0.0	6.682816360683895E-6	0.0	1.1138027267806493E-5
2	1.1138027267806493E-6	4.455210907122597E-6	1.0024224541025843E-5	0.0	2.673126544273558E-5
3	4.455210907122597E-6	4.455210907122597E-6	1.0024224541025843E-5	0.0	4.7893517251567916E-5
4	2.673126544273558E-5	4.455210907122597E-6	2.3389857262393633E-5	1.1138027267806493E-6	5.457633361225181E-5
5	3.5641687256980776E-5	4.455210907122597E-6	3.3414081803419475E-5	1.1138027267806493E-6	5.791774179259376E-5
6	5.791774179259376E-5	4.455210907122597E-6	5.234872815869051E-5	1.1138027267806493E-6	5.791774179259376E-5
7	5.791774179259376E-5	6.682816360683895E-6	8.687661268889064E-5	3.3414081803419475E-6	5.791774179259376E-5
8	7.23971772407422E-5	6.682816360683895E-6	8.799041541567129E-5	3.3414081803419475E-6	6.237295269971636E-5
9	7.796619087464544E-5	6.682816360683895E-6	9.690083722991648E-5	4.455210907122597E-6	7.351097996752285E-5
10-11	8.85473167790616E-5	1.0024224541025843E-5	1.241890040360424E-4	4.455210907122597E-6	8.576280996210999E-5
12-13	1.0803886449772297E-4	1.2251829994587142E-5	1.470219599350457E-4	5.5690136339032465E-6	1.2753041221638434E-4
14-15	1.2196139858248109E-4	1.2251829994587142E-5	1.848912526455878E-4	8.910421814245194E-6	1.447943544814844E-4
16-17	1.264166094896037E-4	1.336563272136779E-5	2.3000026308020406E-4	1.0024224541025843E-5	1.5314787493233927E-4
18-19	1.3477012994045855E-4	1.559323817492909E-5	2.461504026185235E-4	1.2251829994587142E-5	1.5593238174929088E-4
20-21	1.4590815720826506E-4	1.6150139538319416E-5	2.756661748782107E-4	1.336563272136779E-5	1.5593238174929088E-4
22-23	1.5815998720285218E-4	1.6707040901709738E-5	3.185475798592657E-4	1.447943544814844E-5	1.6038759265641348E-4
24-25	1.782084362849039E-4	1.6707040901709738E-5	3.2244588940299795E-4	1.559323817492909E-5	1.6038759265641348E-4
26-27	1.9491547718661361E-4	1.8934646355271035E-5	3.319132125806335E-4	1.7820843628490388E-5	1.609444940198038E-4
28-29	2.2498815080969115E-4	2.060535044544201E-5	3.508478589359045E-4	1.7820843628490388E-5	1.6818421174387803E-4
30-31	2.506056135256461E-4	2.1162251808832336E-5	3.6142898484032063E-4	2.3389857262393633E-5	1.7542392946795227E-4
32-33	2.712109639710881E-4	2.1162251808832336E-5	3.803636311955917E-4	2.4503659989174283E-5	1.837774499188071E-4
34-35	2.9125941305313974E-4	2.2276054535612986E-5	4.3883827435157584E-4	2.4503659989174283E-5	1.9268787173305231E-4
36-37	3.1854757985926566E-4	2.2276054535612986E-5	4.505332029827726E-4	2.4503659989174283E-5	2.0828110990798142E-4
38-39	3.525185630260755E-4	2.283295589900331E-5	4.8171967933263076E-4	2.4503659989174283E-5	2.3055716444359437E-4
40-41	3.786929271054208E-4	2.5060561352564605E-5	5.156906624994406E-4	2.4503659989174283E-5	2.4058138898462022E-4
42-43	4.098794034552789E-4	2.673126544273558E-5	5.357391115814922E-4	2.4503659989174283E-5	2.4559350125513316E-4
44-45	4.304847539007209E-4	2.8401969532906556E-5	5.491047443028601E-4	2.5617462715954933E-5	2.639712462470139E-4
46-47	4.7615066569872753E-4	3.0072673623077528E-5	5.741653056554247E-4	2.673126544273558E-5	2.728816680612591E-4
48-49	5.162475638628309E-4	3.0072673623077528E-5	5.986689656445989E-4	2.673126544273558E-5	2.990560321406043E-4
50-51	5.663686865679602E-4	3.0072673623077528E-5	6.042379792785021E-4	2.784506816951623E-5	3.469495493921722E-4
52-53	6.153760065463087E-4	3.0072673623077528E-5	6.265140338141152E-4	2.784506816951623E-5	3.61985886203711E-4
54-55	6.621557210710959E-4	3.2300279076638825E-5	6.604850169809251E-4	2.784506816951623E-5	3.675548998376142E-4
56-57	6.900007892406122E-4	3.4527884530200126E-5	6.994681124182476E-4	2.895887089629688E-5	3.7312391347151745E-4
58-59	7.356667010386188E-4	3.61985886203711E-5	7.172889560467381E-4	3.4527884530200126E-5	3.8203433528576265E-4
60-61	7.796619087464545E-4	4.009689816410337E-5	7.924706401044319E-4	3.4527884530200126E-5	3.8537574346610463E-4
62-63	8.214295110007287E-4	4.677971452478727E-5	8.904852800611291E-4	4.121070089088402E-5	3.85932644829495E-4
64-65	8.609695078014418E-4	5.123492543190987E-5	9.417202054930389E-4	4.1767602254274346E-5	3.9762757346069175E-4
66-67	8.966111950584226E-4	5.4019432248861486E-5	9.539720354876261E-4	4.232450361766467E-5	4.04867291184766E-4
68-69	9.445047123099906E-4	5.736084042920344E-5	9.81260202293752E-4	4.2881404981054996E-5	4.0765179800171763E-4
70-71	9.968534404686809E-4	5.791774179259376E-5	9.9518273637851E-4	4.343830634444532E-5	4.1600531845257247E-4
72-73	0.0010698075190728137	5.791774179259376E-5	0.0010441900563568586	4.455210907122597E-5	4.304847539007209E-4
74-75	0.0011416477949501656	5.903154451937441E-5	0.0010480883659005908	4.455210907122597E-5	4.388382743515758E-4
76-77	0.0012157156762810787	5.958844588276474E-5	0.001051429774080933	4.455210907122597E-5	4.66683342521092E-4
78-79	0.0012691782071665497	6.404365678988733E-5	0.0010558849849880555	4.51090104346163E-5	4.7670756706211784E-4
80-81	0.0013304373571394854	6.57143608800583E-5	0.0010592263931683975	4.5665911798006615E-5	4.900731997834857E-4
82-83	0.0013783308743910536	7.23971772407422E-5	0.001082616250430791	4.733661588817759E-5	5.056664379584148E-4
84-85	0.0014373624189104278	7.23971772407422E-5	0.0010898559681548651	4.9007319978348566E-5	5.1457685977266E-4
86-87	0.001454626361175528	7.46247826943035E-5	0.0011043354036030135	4.9007319978348566E-5	5.25157985677076E-4
88-89	0.0014590815720826504	7.963689496481642E-5	0.0011087906145101365	5.123492543190987E-5	5.535599552099826E-4
90	0.0014590815720826504	8.130759905498739E-5	0.0011126889240538685	5.123492543190987E-5	5.635841797510085E-4
>>END_MODULE
